[Objective]The study aimed to carry out RAPD analysis of genetic diversity of common and tartary buckwheat varieties.[Method] The genetic diversity of 19 common and tartary buckwheat varieties including the tested var...[Objective]The study aimed to carry out RAPD analysis of genetic diversity of common and tartary buckwheat varieties.[Method] The genetic diversity of 19 common and tartary buckwheat varieties including the tested varieties in Guizhou region during 1999-2010 and their parents were studied using 7 primers by means of random amplified polymorphic DNA(RAPD).[Result]A total of 149 DNA bands were obtained.In which,141 bands were polymorphic,accounting for 94.89%.Polymorphism analysis and cluster analysis showed that all varieties had their own special bands different from each other.The varieties native to Weining were close to each other,and other common buckwheat varieties were obviously different from each other.The interspecific genetic variation was the greatest;the intraspecific genetic variation of common buckwheat varieties was greater than that of tartary buckwheat varieties.[Conclusion]The RAPD fingerprints of the 19 buckwheat varieties were established in present study.展开更多
基金Supported by Special Project of Guizhou Animal and Plant Breeding(QianNongYuZhuanZi[2010]023)Natural Science Foundation of China(31060207)Key Project of Guizhou Agriculture(QianKeHe NY Zi[2010]3094)~~
文摘[Objective]The study aimed to carry out RAPD analysis of genetic diversity of common and tartary buckwheat varieties.[Method] The genetic diversity of 19 common and tartary buckwheat varieties including the tested varieties in Guizhou region during 1999-2010 and their parents were studied using 7 primers by means of random amplified polymorphic DNA(RAPD).[Result]A total of 149 DNA bands were obtained.In which,141 bands were polymorphic,accounting for 94.89%.Polymorphism analysis and cluster analysis showed that all varieties had their own special bands different from each other.The varieties native to Weining were close to each other,and other common buckwheat varieties were obviously different from each other.The interspecific genetic variation was the greatest;the intraspecific genetic variation of common buckwheat varieties was greater than that of tartary buckwheat varieties.[Conclusion]The RAPD fingerprints of the 19 buckwheat varieties were established in present study.