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利用染色体片段置换系定位水稻赖氨酸含量的QTL 被引量:4
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作者 林静 朱镇 +3 位作者 方先文 王艳平 张所兵 王才林 《中国水稻科学》 CAS CSCD 北大核心 2012年第6期737-740,共4页
以籼稻品种9311为受体、粳稻品种日本晴为供体构建95个染色体片段置换系,对水稻控制赖氨酸含量的QTL进行了定位。结果显示,共有7个染色体片段置换系(chromosome segment substitution line,CSSL)的稻米赖氨酸含量与亲本9311差异显著。... 以籼稻品种9311为受体、粳稻品种日本晴为供体构建95个染色体片段置换系,对水稻控制赖氨酸含量的QTL进行了定位。结果显示,共有7个染色体片段置换系(chromosome segment substitution line,CSSL)的稻米赖氨酸含量与亲本9311差异显著。利用代换作图法共鉴定了4个与赖氨酸含量相关的QTL,分别位于水稻第8、9和12染色体上。其中qH-LY8和qHLY9.2来自高赖氨酸含量籼稻品种9311,正向加性效应百分率分别为9.6%和8.5%;而qHLY9.1和qHLY12则来自低赖氨酸含量粳稻品种日本晴,负向加性效应百分率分别为-16.0%和-21.3%。 展开更多
关键词 染色体片段置换系 赖氨酸含量 代换作图 水稻
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利用染色体片段置换系定位水稻米糠油含量的QTL 被引量:2
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作者 林静 张所兵 +2 位作者 张云辉 汪迎节 方先文 《江苏农业学报》 CSCD 北大核心 2015年第2期231-235,共5页
染色体片段置换系由于减少了个体间遗传背景的干扰,已经成为鉴定复杂性状QTL的新型遗传材料。本研究以9311为受体、日本晴为供体的119个染色体片段置换系为试验材料,借助近红外广谱分析技术,对米糠油含量进行了测定,通过差异显著性测验... 染色体片段置换系由于减少了个体间遗传背景的干扰,已经成为鉴定复杂性状QTL的新型遗传材料。本研究以9311为受体、日本晴为供体的119个染色体片段置换系为试验材料,借助近红外广谱分析技术,对米糠油含量进行了测定,通过差异显著性测验及代换作图,鉴定了置换片段上米糠油相关的QTL。以P≤0.001为阈值,共检测到4个米糠油相关的QTLs,其加性效应值的变化范围为0.91%~1.48%,加性效应百分率的变化范围为3.96%~6.46%。 展开更多
关键词 水稻 米糠油含量 染色体片段置换系 QTL定位
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Study on Starch Properties of Rice Enriched with Resistant Starch 被引量:1
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作者 林静 孙宝霞 +3 位作者 方先文 王艳平 张所兵 汪迎节 《Agricultural Science & Technology》 CAS 2014年第3期355-358,378,共5页
This study aimed to compare starch properties among rice germplasms with different contents of resistant starch. Rice germplasms with significant differ- ences in resistant starch content were screened by the rice ger... This study aimed to compare starch properties among rice germplasms with different contents of resistant starch. Rice germplasms with significant differ- ences in resistant starch content were screened by the rice germplasm resource project team in Jiangsu Academy of Agricultural Sciences, to analyze the differences in RVA eigenvalues and starch crystal thermodynamic properties using differential scanning calorimeter (DSC). The result showed that three rice materials with high contents of resistant starch exhibited low breakdown viscosity and high setback vis- cosity; three rice materials with low contents of resistant starch exhibited high breakdown viscosity and low setback viscosity. Significant differences were observed in RVA eigenvalues and starch crystal thermodynamic properties among rice germplasms with different contents of resistant starch, which provided new indices for breeding functional rice cultivars with high resistant starch content. 展开更多
关键词 RICE Resistant starch RVA eigenvalue Starch crystal thermodynamics
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Mapping QTLs Associated with Sheath Blight Resistance Using Chromosome Segment Substitution Lines of Rice(Oryza sativa L.)
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作者 林静 张所兵 +2 位作者 张云辉 汪迎节 方先文 《Agricultural Science & Technology》 CAS 2014年第5期756-759,共4页
In this study, a population of 119 chromosome segment substitution lines (CSSLs) derived from backcross between indica 9311 and japonica Nipponbare was employed to map quantitative trait loci (QTL) associated with... In this study, a population of 119 chromosome segment substitution lines (CSSLs) derived from backcross between indica 9311 and japonica Nipponbare was employed to map quantitative trait loci (QTL) associated with sheath blight resis-tance in rice with toothpick inoculation method. A total of three sheath blight resis-tance-associated QTLs (qsb8-1, qsb8-2 and qsb8-3) were identified, which were lo-cated on adjacent molecular markers RM3262, RM5485 and RM3496 of chromo-some 8; the genetic interval was 81.7cM-91.7cM, 91.7cM-108.1cM and 108.1cM-119.6cM, respectively. The additive effect of qsb8-2 was negative, indicating that sheath blight resistance of susceptible parent harboring qsb8-2 fragment was en-hanced; additive effects of qsb8-1 and qsb8-3 were positive, indicating that sheath blight resistance of susceptible parent harboring qsb8-1 and qsb8-3 fragments was reduced. 展开更多
关键词 RICE Sheath blight resistance Chromosome segment substitution lines(CSSLs) Quantitative trait locus (QTL) mapping
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