建立液相色谱—串联质谱法(LC-MS/MS)检测乙氧磺隆和双环磺草酮在水稻中残留分析方法。样品经乙腈溶液涡旋提取,NaCl盐析,0.25 g MgSO4、0.05 g PSA和0.05 g C18净化后,采用电喷雾离子源正离子扫描,多反应监测(MRM)模式下,外标法定量。...建立液相色谱—串联质谱法(LC-MS/MS)检测乙氧磺隆和双环磺草酮在水稻中残留分析方法。样品经乙腈溶液涡旋提取,NaCl盐析,0.25 g MgSO4、0.05 g PSA和0.05 g C18净化后,采用电喷雾离子源正离子扫描,多反应监测(MRM)模式下,外标法定量。结果表明:在0.001~0.5μg/mL范围内线性关系良好,相关系数(R2)>0.99。当添加浓度为0.01~1.0 mg/kg时,在糙米中的平均回收率为84%~107%,相对标准偏差为1%~8%。当添加浓度为0.05~2.5 mg/kg时,在0.001~0.5μg/mL范围内线性关系良好,相关系数(R2)>0.99。在稻壳中的平均回收率为86%~101%,相对标准偏差为2%~8%;在秸秆中的平均回收率为87%~99%,相对标准偏差为6%~11%。乙氧磺隆和双环磺草酮在糙米中的定量限为0.01 mg/kg,在稻壳和秸秆中的定量限为0.05 mg/kg。展开更多
[Objective] The nematicidal activity of plant extracts was investigated. We aimed to explore a kind of botanical pesticide to control Hirschmanniella spp. [Method] The Chuanxiong （root）, agrimony （whole plant）, co...[Objective] The nematicidal activity of plant extracts was investigated. We aimed to explore a kind of botanical pesticide to control Hirschmanniella spp. [Method] The Chuanxiong （root）, agrimony （whole plant）, cocklebur （grain）, basil （leaves）, ragweed （stems and leaves）, green onion （root）, garlic （tuber）, Yuzhu （leaves）, marshpeper smartweed （whole plant）, horseweed （aboveground stems and leaves） were dried, grinded and sieved. The powders were then extracted by ethanol at room temperature for 24 h. The extract solutions were filtered through fil- ter pater. The obtained plant extracts were then diluted to determine their nematici- dal activities. [Result] The Chuanxiong extract had the highest nematicidal activity, followed by cocklebur extract, agrimony extract, basil extract, garlic extract, ragweed extract. The horseweed extract had the lowest nematicidal activity. [Conclusion] The Chuanxiong and other plants were natural, and they had a good environmental compatibility. They could be developed as botanical pesticides against Hirschman- niella spp.展开更多
In order to improve the utilization efficiency of hybrid rice seeds, the effects of seed-coating agents on hybrid rice seeds with dehiscent glumes were investigated. Total six kinds of seed-coating agents were selecte...In order to improve the utilization efficiency of hybrid rice seeds, the effects of seed-coating agents on hybrid rice seeds with dehiscent glumes were investigated. Total six kinds of seed-coating agents were selected, i.e., 20.5% imidacloprid ·prochloraz FS, 30.5% imidacloprid oprochloraz FS, 23.0% thiamethoxam · hymexazol·prochloraz FS, Ruisheng WS, Shileshi FS and Liangdun FS, and their effects of seeds of Longliangyou Huazhan and T You 272 were investigated. The results showed that when the percentage of glume-dehiscent seeds reached 10%, 20.5% imidacloprid .prochloraz significantly improved the germination rate of Longliangyou Huazhan, which was 4.33% higher than that in the control group, and the seedling rate of Longliangyou Huazhan remained unchanged; 30.5% imidacloprid. prochloraz did not improved the germination rate of T You 272, but improved its seedling rate, which was 7.66% higher than that in the control group. When the percentage of glume-dehiscent seeds reached 20%, 20.5% imidacloprid ·prochloraz and 30.5% imidacloprid.prochloraz improved the germination rate of T You 272 by 3.50% and 4.83%, respectively, and its seedling rate did not decline. In conclusion, seed-coating agents have certain repair effect on glume-dehiscent rice seeds; 20.5% imidacloprid ·prochloraz and 30.5% imidacloprid ·prochloraz could improve the efficiency of Longliangyou Huazhan and T You 272 with small amounts of glume-dehiscent seeds.展开更多
文摘建立液相色谱—串联质谱法(LC-MS/MS)检测乙氧磺隆和双环磺草酮在水稻中残留分析方法。样品经乙腈溶液涡旋提取,NaCl盐析,0.25 g MgSO4、0.05 g PSA和0.05 g C18净化后,采用电喷雾离子源正离子扫描,多反应监测(MRM)模式下,外标法定量。结果表明:在0.001~0.5μg/mL范围内线性关系良好,相关系数(R2)>0.99。当添加浓度为0.01~1.0 mg/kg时,在糙米中的平均回收率为84%~107%,相对标准偏差为1%~8%。当添加浓度为0.05~2.5 mg/kg时,在0.001~0.5μg/mL范围内线性关系良好,相关系数(R2)>0.99。在稻壳中的平均回收率为86%~101%,相对标准偏差为2%~8%;在秸秆中的平均回收率为87%~99%,相对标准偏差为6%~11%。乙氧磺隆和双环磺草酮在糙米中的定量限为0.01 mg/kg,在稻壳和秸秆中的定量限为0.05 mg/kg。
基金Supported by Science and Technology Project of Hunan Provincial Department of Education(11A059)Earmarked Fund for China Agriculture Research System(CARS19-05B)~~
文摘[Objective] The nematicidal activity of plant extracts was investigated. We aimed to explore a kind of botanical pesticide to control Hirschmanniella spp. [Method] The Chuanxiong （root）, agrimony （whole plant）, cocklebur （grain）, basil （leaves）, ragweed （stems and leaves）, green onion （root）, garlic （tuber）, Yuzhu （leaves）, marshpeper smartweed （whole plant）, horseweed （aboveground stems and leaves） were dried, grinded and sieved. The powders were then extracted by ethanol at room temperature for 24 h. The extract solutions were filtered through fil- ter pater. The obtained plant extracts were then diluted to determine their nematici- dal activities. [Result] The Chuanxiong extract had the highest nematicidal activity, followed by cocklebur extract, agrimony extract, basil extract, garlic extract, ragweed extract. The horseweed extract had the lowest nematicidal activity. [Conclusion] The Chuanxiong and other plants were natural, and they had a good environmental compatibility. They could be developed as botanical pesticides against Hirschman- niella spp.
基金Supported by Production-study-research Cooperation Projects of Hunan University of Humanities(2014CXY06,2013CXY04)Industrial Incubation Projects for Universities and Colleges of Hunan Province(13CY030)~~
文摘In order to improve the utilization efficiency of hybrid rice seeds, the effects of seed-coating agents on hybrid rice seeds with dehiscent glumes were investigated. Total six kinds of seed-coating agents were selected, i.e., 20.5% imidacloprid ·prochloraz FS, 30.5% imidacloprid oprochloraz FS, 23.0% thiamethoxam · hymexazol·prochloraz FS, Ruisheng WS, Shileshi FS and Liangdun FS, and their effects of seeds of Longliangyou Huazhan and T You 272 were investigated. The results showed that when the percentage of glume-dehiscent seeds reached 10%, 20.5% imidacloprid .prochloraz significantly improved the germination rate of Longliangyou Huazhan, which was 4.33% higher than that in the control group, and the seedling rate of Longliangyou Huazhan remained unchanged; 30.5% imidacloprid. prochloraz did not improved the germination rate of T You 272, but improved its seedling rate, which was 7.66% higher than that in the control group. When the percentage of glume-dehiscent seeds reached 20%, 20.5% imidacloprid ·prochloraz and 30.5% imidacloprid.prochloraz improved the germination rate of T You 272 by 3.50% and 4.83%, respectively, and its seedling rate did not decline. In conclusion, seed-coating agents have certain repair effect on glume-dehiscent rice seeds; 20.5% imidacloprid ·prochloraz and 30.5% imidacloprid ·prochloraz could improve the efficiency of Longliangyou Huazhan and T You 272 with small amounts of glume-dehiscent seeds.
