基于体外细胞模型的太子参安全性与抗氧化活性系统评价

为科学认识和系统评价太子参提取物在细胞水平上的安全性与抗氧化活性,根据PICOS检索策略检索国内外数据库,采用Stata软件进行Meta循证统计分析,共纳入11篇文献18项研究,总样本量为482。Meta分析显示:太子参试验组体外细胞毒性试验结果较对照组差异不显著[SMD=0.22,95%CI(-0.22,0.66),P=0.219>0.05];太子参提取物可显著提高体外试验细胞存活率[SMD=0.96,95%CI(0.15,1.77),P<0.05]和极显著降低细胞凋亡率[SMD=-2.38,95%CI(-3.21,-1.54),P<0.01],并且极显著提升胞内超氧化物歧化酶(SOD)活力[SMD=1.65,95%CI(0.97,2.32),P<0.01]和极显著降低胞内丙二醛(MDA)含量[SMD=-1.99,95%CI(-2.68,-1.29),P<0.01];对抑制体外红细胞溶血的系统描述结果为阳性,敏感分析结果稳健,发表偏倚弱。基于现有文献显示:太子参提取物在细胞水平上具有良好的安全性和抗氧化活性。

太子参须提取物对小鼠免疫功能的影响

试验旨在探究太子参须提取物对小鼠免疫功能的影响,分别以太子参须提取物低(0.1 g/kg)、中(0.2 g/kg)、高(0.4 g/kg)三个剂量梯度给药量灌胃小鼠,记录小鼠体质量并检测小鼠免疫指标。结果显示:与空白对照组相比,低剂量组小鼠的体质量和脾脏指数均显著提高(P<0.05),中剂量组小鼠的T细胞刺激指数显著升高(P<0.05),高剂量组小鼠的胸腺指数和巨噬细胞吞噬指数均显著提高(P<0.05)。研究表明,太子参须提取物可显著改善小鼠免疫功能。

Effects of Different Extract of Pseudostellaria Heterophylla on Immunological Function in Mice based on Meta-analysis and Network Meta-analysis

To evaluate the effects of different extract of Pseudostellaria heterophylla on immunological function in mice based on Meta-analysis and Network meta-analysis,the article retrieved domestic and foreign databases according to the"PICO"retrieval strategy,and used Stata and ADDIS software for Meta analysis.A total of 6 reports,10 randomised controlled trials(RCTs)were eventually involved.Meta analysis results showed that:compared with the control group,the experimental group of polysaccharide,saponin and crude extract were better than that of the control(P<0.05),which significantly improved the immunological function in mice.Network meta-analysis results showed that the saponin had the best effect on the increase of phagocytic index and the differences were statistically significant[MD=1.50,95%CI(0.71,2.28),P<0.05];The polysaccharide and saponin had better effect on the increase of spleen index than the control and crude extract,and the differences were statistically significant[MD=0.77,95%CI(0.27,1.31),P<0.05],[MD=0.71,95%CI(0.01,1.50),P<0.05];the polysaccharide had the best effect on the increase of thymus index and the differences were statistically significant[MD=0.70,95%CI(0.11,1.34),P<0.05].The rank probability showed that the saponin of Pseudostellaria heterophylla had the maximum probability to increase phagocytic index of mice;the probability for the components of Pseudostellaria heterophylla increasing spleen index of mice was in the order of crude extract>polysaccharide>saponin;the probability for the components of Pseudostellaria heterophylla increasing thymus index of mice was in the order of saponin>polysaccharide>crude extract.Based on the available evidence,the extract of Pseudostellaria heterophylla could improve the immunity of mice,and the clinical effect of polysaccharide and saponin was the best,which provided a more valuable scientific reference for evidencing that the polysaccharide and saponin of Pseudostellaria heterophylla was the effective components for improving immunological function,and also was conducive to the proper further development of Pseudostellaria heterophylla resources.

Optimization of Steam Explosion Process Condition for Extracting Polysaccharides from Pseudostellaria heterophylla by Response Surface Methodology

【Objective】To optimize the steam explosion process condition for extracting polysaccharides from Pseudostellaria heterophylla.【Method】The effects of steam pressure,pressure-maintaining time and material moisture content on the extraction of polysaccharides from Pseudostellaria heterophylla were studied by response surface methodology based on Box-Behnken design.【Result】The findings showed that each factor could significantly affect the test index,and the optimum condition was as follows:steam pressure 1.50 Mpa,pressuremaintaining time 46 s and material moisture content 46%.Under this condition,the verified experimental value of polysaccharides from Pseudostellaria heterophylla was 39.32%,indicating a relative standard deviation of 2.73%from the predictive value.Meanwhile,scanning electron microcopy(SEM)images showed that the surface physical structure of Pseudostellaria heterophylla was irregularly broken and cracked,which means the physical structure of Pseudostellaria heterophylla was changed and destroyed at the cellular level.【Conclusion】This experiment provides a new approach for the extraction of polysaccharides from Pseudostellaria heterophylla,as well as a reference for the resource utilization of Pseudostellaria heterophylla.

补益中药太子参抗氧化活性及其作用机理研究进展

太子参为石竹科植物孩儿参的干燥块根,始载于清代《本草从新》,主产于福建、贵州和江苏等地[1]。太子参兼有药用和营养双重功效,富含多糖、皂苷、环肽等组分,临床证明其具有健脾益肺、抗氧化、抗炎、增强免疫力等作用[2-3]。

可视化分析国内养殖环境类研究热点

随着人们健康化水平的提升,对畜牧产品的需求不断攀升,预计到2050年人们对肉类产品的需求量将增加73%,因此会快速推进畜牧养殖业集约化和规模化的发展进程。相关数据资料显示,我国畜牧业已连续20年以平均9.9%的增速递增,约占农业总产值的33.3%,而我国畜禽养殖产生的粪污由1999年的19亿吨迅速增至2010年的40亿吨,但粪污无公害化处理不足10%,超过80%的养殖粪污存在场外存放或未处理现象。

一株除臭乳酸菌的筛选及应用效果

为获得具有除臭功能的养殖用微生态制剂备用菌株,从畜禽养殖场粪便富集培养后分离的乳酸菌中,筛选出一株除臭乳酸菌BDLC-3,经16SrDNA测序分析,鉴定为短乳杆菌(lactobacillus brevis),通过大鼠经口毒性试验结果表明该菌株安全无毒。对菌株发酵制备获得的菌剂进行应用效果检验,结果显示,菌剂BDLC-3以3%的接种量处理新鲜猪粪7d可达到最优的除臭效果,对氨气和硫化氢的去除率分别为73.8%±2.6%和55.8%±3.1%。结果表明该菌株具有良好的生长性能、安全性和除臭性能,具备在畜禽养殖中作为益生、除臭一体微生态制剂的潜在应用价值。