The immune responses of plants to foreign pathogens have developed relevant defense mechanisms, which formed complicated disease resistant signal transduction pathways. Salicylic acid(SA), jasmonic acid(JA)/Ethyl...The immune responses of plants to foreign pathogens have developed relevant defense mechanisms, which formed complicated disease resistant signal transduction pathways. Salicylic acid(SA), jasmonic acid(JA)/Ethylene(ET) and brassi- nosteroid (BR) could trigger the immune response to different pathogens in plants, making the plants show some resistance to the pathogens. The study on the trans- duction pathways of these three disease-resistant signals were introduced to provide some useful suggestions for the study on the transduction of disease-resistant sig- nals in plants.展开更多
文摘在许多渗透调节剂中,甜菜碱是最理想的有机小分子渗透调节物质。甜菜碱在植物体内大量积累不会带来危害,同时能提高植物对环境胁迫的抗性。将海马齿中克隆到的甜菜碱醛脱氢酶基因构建到表达载体p ET-28a(+)上,获得重组载体p ET-Sp BADH并将其成功地转化到BL21(DE3)中得到重组工程菌,经IPTG诱导能高效表达55 k D目的蛋白,表达量可以达到301μg m L–1。酶学特征分析表明,该蛋白最适p H值为7.2,在偏碱条件下能维持较高的催化活性;Sp BADH蛋白对高温敏感,且温度对催化活性影响较大,超过55℃时酶活性只有20%,最适酶催化活性温度为37℃;而有机小分子醇类对酶的催化活性有保护作用,可以通过自身特征维持酶催化活性的微环境。
基金Supported by the National Natural Science Foundation of China(31360262)Zhuke Contract(2012HK209-38)the Innovation Capacity Platform Construction Project of Guizhou Science and Technology Department(2011018)~~
文摘The immune responses of plants to foreign pathogens have developed relevant defense mechanisms, which formed complicated disease resistant signal transduction pathways. Salicylic acid(SA), jasmonic acid(JA)/Ethylene(ET) and brassi- nosteroid (BR) could trigger the immune response to different pathogens in plants, making the plants show some resistance to the pathogens. The study on the trans- duction pathways of these three disease-resistant signals were introduced to provide some useful suggestions for the study on the transduction of disease-resistant sig- nals in plants.