Myoglobin was immobilized on edge-plane pyrolytic graphite（EPG）electrodes by sodium alginate hydrogel.The protein entrapped in the sodium alginate film undergoes fast direct electron-transfer reactions with the form...Myoglobin was immobilized on edge-plane pyrolytic graphite（EPG）electrodes by sodium alginate hydrogel.The protein entrapped in the sodium alginate film undergoes fast direct electron-transfer reactions with the formal potential(Eo′)of-0.296V（vs SCE）,corresponding to FeⅢ+e-→FeⅡ,The Eo′s are linearly dependent on solution pH with the slope value of-35.0 mV/pH,indicating that the electron transfer was proton-coupled.The mechanisms for catalytic reduction of oxygen,hydrogen peroxide and nitric oxide by myoglobin entrapped in sodium alginate hydrogel were also explored.展开更多
文摘Myoglobin was immobilized on edge-plane pyrolytic graphite（EPG）electrodes by sodium alginate hydrogel.The protein entrapped in the sodium alginate film undergoes fast direct electron-transfer reactions with the formal potential(Eo′)of-0.296V（vs SCE）,corresponding to FeⅢ+e-→FeⅡ,The Eo′s are linearly dependent on solution pH with the slope value of-35.0 mV/pH,indicating that the electron transfer was proton-coupled.The mechanisms for catalytic reduction of oxygen,hydrogen peroxide and nitric oxide by myoglobin entrapped in sodium alginate hydrogel were also explored.
