Proteins are a class of biomaterials having a vast array of functions, including the catalysis of metabolic reactions, DNA replication, stimuli response and transportation of molecules. Recent progress in laser-based...Proteins are a class of biomaterials having a vast array of functions, including the catalysis of metabolic reactions, DNA replication, stimuli response and transportation of molecules. Recent progress in laser-based fabrication technologies has enabled the formation of three-dimensional （3D） proteinaceous micro- and nano-structures by femtosecond laser cross-linking, which has expanded the possible applications of proteins. This article reviews the current knowledge andrecent advancements in the femtosecond laser cross-linking of proteins. An overview of previous studies related to fabri-cation using a variety of proteins and detailed discussions of the associated mechanisms are provided. In addition, ad-vances and applications utilizing specific protein functions are introduced. This review thus provides a valuable summaryof the 3D micro- and nano-fabrication of proteins for biological and medical applications.展开更多
Surface-enhanced Raman scattering(SERS),owing to its high sensitivity based on localized surface plasmon resonance of nanostructured metals,is recently attracting much attention to be used for biotechnology,such as ce...Surface-enhanced Raman scattering(SERS),owing to its high sensitivity based on localized surface plasmon resonance of nanostructured metals,is recently attracting much attention to be used for biotechnology,such as cell imaging and tumor therapy.On the other hand,the trace detection of bio-molecules with large molecular weight is still challenging because the troublesome treatment of SERS substrate using coupling or cross-linking agents is required.In this paper,we apply liquid interface assisted SERS(LI-SERS)method,which provides unique features of collection and self-immobilization of analyte molecules on the SERS substrate,to realize the label-free trace detection of bio-molecules with detection limits of pM~fM.Specifically,deoxyribonucleic acid(DNA)discrimination and quantitative detection ofβ-Amyloid(Aβ)in trace-concentration are demonstrated to illustrate the ultrahigh sensitivity and versatility of the LI-SERS method.The results suggest LI-SERS is promising for the early-stage diagnosis of diseases such as virus infection and Alzheimer's disease.展开更多
Surface-enhanced Raman scattering(SERS)techniques have rapidly advanced over the last two decades,permitting multidisciplinary trace analyses and the potential detection of single molecules.This paper provides a compr...Surface-enhanced Raman scattering(SERS)techniques have rapidly advanced over the last two decades,permitting multidisciplinary trace analyses and the potential detection of single molecules.This paper provides a comprehensive review of recent progress in strategies for the fabrication of highly sensitive SERS substrates,as a means of achieving sensing on the attomolar scale.The review examines widely used performance criteria,such as enhancement factors.In addition,femtosecond laser-based techniques are discussed as a versatile tool for the fabrication of SERS substrates.Several approaches for enhancing the performance of SERS sensing devices are also introduced,including photo-induced,transient,and liquid-interface assisted strategies.Finally,substrates for real-time sensing and biological applications are also reviewed to demonstrate the powerful analytical capabilities of these methods and the significant progress in SERS research.展开更多
文摘Proteins are a class of biomaterials having a vast array of functions, including the catalysis of metabolic reactions, DNA replication, stimuli response and transportation of molecules. Recent progress in laser-based fabrication technologies has enabled the formation of three-dimensional （3D） proteinaceous micro- and nano-structures by femtosecond laser cross-linking, which has expanded the possible applications of proteins. This article reviews the current knowledge andrecent advancements in the femtosecond laser cross-linking of proteins. An overview of previous studies related to fabri-cation using a variety of proteins and detailed discussions of the associated mechanisms are provided. In addition, ad-vances and applications utilizing specific protein functions are introduced. This review thus provides a valuable summaryof the 3D micro- and nano-fabrication of proteins for biological and medical applications.
基金the Special Postdoctoral Researcher Program and Incentive Research Project of RIKEN.
文摘Surface-enhanced Raman scattering(SERS),owing to its high sensitivity based on localized surface plasmon resonance of nanostructured metals,is recently attracting much attention to be used for biotechnology,such as cell imaging and tumor therapy.On the other hand,the trace detection of bio-molecules with large molecular weight is still challenging because the troublesome treatment of SERS substrate using coupling or cross-linking agents is required.In this paper,we apply liquid interface assisted SERS(LI-SERS)method,which provides unique features of collection and self-immobilization of analyte molecules on the SERS substrate,to realize the label-free trace detection of bio-molecules with detection limits of pM~fM.Specifically,deoxyribonucleic acid(DNA)discrimination and quantitative detection ofβ-Amyloid(Aβ)in trace-concentration are demonstrated to illustrate the ultrahigh sensitivity and versatility of the LI-SERS method.The results suggest LI-SERS is promising for the early-stage diagnosis of diseases such as virus infection and Alzheimer's disease.
文摘Surface-enhanced Raman scattering(SERS)techniques have rapidly advanced over the last two decades,permitting multidisciplinary trace analyses and the potential detection of single molecules.This paper provides a comprehensive review of recent progress in strategies for the fabrication of highly sensitive SERS substrates,as a means of achieving sensing on the attomolar scale.The review examines widely used performance criteria,such as enhancement factors.In addition,femtosecond laser-based techniques are discussed as a versatile tool for the fabrication of SERS substrates.Several approaches for enhancing the performance of SERS sensing devices are also introduced,including photo-induced,transient,and liquid-interface assisted strategies.Finally,substrates for real-time sensing and biological applications are also reviewed to demonstrate the powerful analytical capabilities of these methods and the significant progress in SERS research.
