In this paper, we first presented a novel method for quantification of circulating DNA in human serum based on capillary zone electrophoresis with laser-induced fluorescence detection (CZE-LIF). The serum was digeste...In this paper, we first presented a novel method for quantification of circulating DNA in human serum based on capillary zone electrophoresis with laser-induced fluorescence detection (CZE-LIF). The serum was digested by proteinase to release free DNA, and then CZE-LIF system was used for the quantification of total circulating DNA. This method was successfully used to quantify the circulating DNA levels in sera from healthy individuals and certain cancer patients. We found the significantly elevated circulating DNA levels in certain prostate cancer patients. Our results demonstrated that CZE-LIF system has good linearity, excellent sensitivity (0.5 ng/mL DNA), satisfactory reproducibility (RSDs in one day and between days were both less than 5%) and reliability, and is well suitable to the quantification of the circulating DNA in human serum or plasma.展开更多
Objective:To analyze the epidemiological data of trauma caused by traffic accidents on Hefei-Nanjing expressway from the year of 1995 to 1996.Methods:The data provided by Anhui Provincial Traffic Police Bureau were an...Objective:To analyze the epidemiological data of trauma caused by traffic accidents on Hefei-Nanjing expressway from the year of 1995 to 1996.Methods:The data provided by Anhui Provincial Traffic Police Bureau were analyzed and discussed. Rsults:Mortality rates in 1995 and in 1996 were respectively 0.19 and 0.12 er 10000vehicles.Traffic accidents occurred mostly in fine weather from 22:00 to 6:00.The cause of traffic acidents in the first place was the head-tail collision,then the collision with fixtures or overturning,Traffic accidents bringing about by drivers accounted for more than 70 percent of the cases.The reasons was weary driving,illegal parking and over-speed driving.The breakdown of vehicles and pedestrians on highway were also the cause of accidents.Conclusions:In order to reduce the incidence of traum caused by traffic accidents on expressway,the followings should be carried out as strengthening the policy or traffic administration on expressway,examining strictly vehicles annually,educating drivers to abide by traffic rules,propagandizing people's traffic safety consciousness and forbidding pedestrians to walk into expressways.展开更多
文摘In this paper, we first presented a novel method for quantification of circulating DNA in human serum based on capillary zone electrophoresis with laser-induced fluorescence detection (CZE-LIF). The serum was digested by proteinase to release free DNA, and then CZE-LIF system was used for the quantification of total circulating DNA. This method was successfully used to quantify the circulating DNA levels in sera from healthy individuals and certain cancer patients. We found the significantly elevated circulating DNA levels in certain prostate cancer patients. Our results demonstrated that CZE-LIF system has good linearity, excellent sensitivity (0.5 ng/mL DNA), satisfactory reproducibility (RSDs in one day and between days were both less than 5%) and reliability, and is well suitable to the quantification of the circulating DNA in human serum or plasma.
文摘Objective:To analyze the epidemiological data of trauma caused by traffic accidents on Hefei-Nanjing expressway from the year of 1995 to 1996.Methods:The data provided by Anhui Provincial Traffic Police Bureau were analyzed and discussed. Rsults:Mortality rates in 1995 and in 1996 were respectively 0.19 and 0.12 er 10000vehicles.Traffic accidents occurred mostly in fine weather from 22:00 to 6:00.The cause of traffic acidents in the first place was the head-tail collision,then the collision with fixtures or overturning,Traffic accidents bringing about by drivers accounted for more than 70 percent of the cases.The reasons was weary driving,illegal parking and over-speed driving.The breakdown of vehicles and pedestrians on highway were also the cause of accidents.Conclusions:In order to reduce the incidence of traum caused by traffic accidents on expressway,the followings should be carried out as strengthening the policy or traffic administration on expressway,examining strictly vehicles annually,educating drivers to abide by traffic rules,propagandizing people's traffic safety consciousness and forbidding pedestrians to walk into expressways.
