A comparative assessment on regeneration status of indigenous woody plants in Eucalyptus grandis plantation and adjacent natural forest

Diversity, density and species composition of naturally regenerated woody plants under Eucalyptus grandis plantation and the adjacent natural forest were investigated and compared. Twenty plots, with an area of 20 m × 20 m for each, were established in both of E. grand＆ plantation and adjacent natural forest, independently. In each plot, species name, abundance, diameter and height were recorded. Numbers of seedling were collected in five sub-plots （4 m2） within each major plot. A total of 46 species in the plantation, and 52 species in the natural forest, which belongs to 36 families were recorded. The diversity of species （H＇） is 2.19 in the plantation and 2.74 in the natural forest. The density of understory woody plant was 3842 stems/ha in the plantation and 4122 stems/ha in the natural forest. The densities of seedlings in the natural forest and the plantation were 8101 stems/ha and 4151 stems/ha, respectively. High similarity of woody species composition was found between the natural forest and the plantation. The E. grand＆ plantation was found favoring the regeneration and growth of Millitiaferruginia and Coffea arabica in a much better way than other underneath woody species.

Anti-osteoarthritis effect of a combination treatment with human adipose tissue-derived mesenchymal stem cells and thrombospondin 2 in rabbits

BACKGROUND Osteoarthritis(OA),a chronic age-related disease characterized by the slowly progressive destruction of articular cartilage,is one of the leading causes of disability.As a new strategy for treatment of OA,mesenchymal stem cells(MSCs)have the potential for articular cartilage regeneration.Meanwhile,thrombospondin 2(TSP2)promotes the chondrogenic differentiation of MSCs.AIM To investigate whether TSP2 induces chondrogenic differentiation of human adipose-derived MSCs(hADMSCs)and potentiates the therapeutic effects of hADMSCs in OA rabbits.METHODS We investigated the chondrogenic potential of TSP2 in hADMSCs by analyzing the expression of chondrogenic markers as well as NOTCH signaling genes in normal and TSP2 small interfering RNA(siRNA)-treated stem cells.Anterior cruciate ligament transection surgery was performed in male New Zealand white rabbits,and 8 wk later,hADMSCs(1.7×10^6 or 1.7×10^7 cells)were injected into the injured knees alone or in combination with intra-articular injection of TSP2(100 ng/knee)at 2-d intervals.OA progression was monitored by gross,radiological,and histological examinations.RESULTS In hADMSC culture,treatment with TSP2 increased the expression of chondrogenic markers(SOX9 and collagen Ⅱ)as well as NOTCH signaling genes(JAGGED1 and NOTCH3),which were inhibited by TSP2 siRNA treatment.In vivo,OA rabbits treated with hADMSCs or TSP2 alone exhibited lower degree of cartilage degeneration,osteophyte formation,and extracellular matrix loss 8 wk after cell transplantation.Notably,such cartilage damage was further alleviated by the combination of hADMSCs and TSP2.In addition,synovial inflammatory cytokines,especially tumor-necrosis factor-α,markedly decreased following the combination treatment.CONCLUSION The results indicate that TSP2 enhances chondrogenic differentiation of hADMSCs via JAGGED1/NOTCH3 signaling,and that combination therapy with hADMSCs and TSP2 exerts synergistic effects in the cartilage regeneration of OA joints.

Patterns of Genetic Diversity and Population Structure of the Clonal Herb, Potentilla fragarioides var. sprengeliana (Rosaceae) in Korea

The genetic diversity and population structure of Potentilla fragarioides var. sprengeliana (Rosaceae) in Korea were investigated using genetic variation at 22 allozyme loci. The percent of polymorphic loci within the enzymes was 59.1%. The genetic diversity at the species level and at the population level was high ( H es =0.210; H ep =0.199, respectively), whereas the extent of the population divergence was relatively low ( G ST =0.074). F IS , a measure of the deviation from random mating within the 19 populations, was 0.331 . An indirect estimate of the number of migrants per generation ( N m =3.15) indicates that gene flow is high among Korean populations of the species. In addition, analysis of fixation indices revealed a slight heterozygote deficiency in some populations and at some loci. The mean genetic identity between populations was 0.985. It is highly probable that the trend of genetic uniformity in a relatively homogeneous habitat is thought to be operated among Korean populations of P. fragarioides var. sprengeliana .

Effect of SP600125 on proliferation of embryonic stem cell

SP600125 is an inhibitor of c-Jun NH2-terminal kinase (JNK), which plays a fundamental role in regulating animal development. Using SP600125 to deal with the mouse embryonic stem cells, it is revealed that the number of the ESC colonies decreased and the size became smaller. With treatment by SP600125, the proliferation of mouse ES cells is seriously inhibited for the cycle arrested in the G2/M phase, and the effect of SP600125 on the ES cells displays correlation of dose and time. The obtained results indicate that JNK may be an important regulator in the progression of cell cycle at the G2/M cell phase for the ES cells.

Molecular phylogenetic analyses based on the complete plastid genomes and nuclear sequences reveal Daphne(Thymelaeaceae)to be non-monophyletic as current circumscription

The diverse members of the genus Daphne are prized for their fragrant flowers.Despite being promising ornamental plants in many countries,genetic information of Daphne is scarce.In this study,the plastomes of four species and one variety of Daphne were sequenced and analyzed.The plastomes were typical and contained a pair of inverted repeat(IR)regions that separated the large single-copy(LSC)region from the small single-copy(SSC)region.With a length ranging from 132,869 bp(D.genkwa)to 174,773 bp(D.championii),106 to 141 genes were predicted.Comparative plastome analysis of the newly sequenced plastomes with four publicly available Daphne plastomes identified an expansion of the IRs,sequence variations,and mutational hotspots.Phylogenetic analyses indicated that the genus Daphne in its current circumscription is polyphyletic.Daphne genkwa was nested within the genus Wikstroemia,while D.championii was well resolved as sister to Edgeworthia.These findings concurred with results from our study that used nuclear ribosomal internal transcribed spacer sequence data.The conflicts on the molecular placement of D.championii and D.genkwa and the present taxonomic classification in Daphne suggest that a new intergeneric classification system of Daphneae warrants consideration.

Potential neoplastic effects of parathion-methyl on rat liver

The mutagenic and carcinogenic effects of parathion-methyl were examined by bacterial reverse mutation assay and a long-term experiment with wistar rats. The potential mutagenic effect of parathion-methyl in Salmonella typhimurium TA100 bacterial cells was observed without rat liver S9 metabolic activation. Parathion-methyl was further investigated for pathological changes in rat pancreas and liver. The long-term rat experiments showed that parathion-methyl exposure for 3 months can cause pathological changes in rat pancreases acinar cells and pancreatic hepatocytes. Atypical acinar cell focuses （AACF） were determined in the liver and pancreas of the rats. The results from short-term Ames test and long-term rat experiments suggested that parathion-methyl would be potential carcinogenic.

Macro-and Microelement Contents of Fruiting Bodies of Wild-Edible Mushrooms Growing in the East Black Sea Region of Turkey

Eleven different wild-edible mushroom species growing in the Black Sea region of Turkey were analysed for their metal content. Specimens of mushrooms were gathered in Trabzon, Giresun and Ordu and analyzed for 31 minerals, four of which (Be, Sb, Te and Ti) were not detected. Whereas some minerals including Ag, As, Cd, La, Mo, Pb, Se, Y and Zr were detected in just a few mushroom species, another 18 minerals were found in all 11 species. All metal concentrations were expressed on a dry weight basis (d.w.). With regard to nutritionally important amounts of essential and trace minerals, the metal content (μg/g d.w.) of mushroom samples ranged from 21,800-39,800 for K, 2590-14,000 for P, 268-1600 for Ca, 561-1210 for Mg, 74-829 for Fe, 11.2-321 for Cu, 36.2-241 for Zn, 14.1-76.5 for Mn and 0.13-2.85 for Co. Small amounts of toxic metals such as As, Cd and Pb were found in all 11 mushroom species. L. laccata contained a large amount of As (145 μg/g d.w.). These results show that the investigated mushrooms can be a useful component for human diets because of their high content of many essential minerals and trace elements and low content of toxic metals.

Do Really Close Stomata by Soil Drying ABA Produced in the Roots and Transported in Transpiration Stream?

Stomatal aperture responses of Commelina communis L. between well watered plants and water stressed plants were investigated. To see the very rapid response to water stress, the plants were directly rooted out from the soil and exposed to the air immediately. Stomata, rooted out from the soil, were totally closed within 10 minutes without any detention time while the stomata of the plants in the soil had been kept opening. These results suggest that stomatal response to the abrupt water stress is very rapid indicating that guard cell itself or leaves could sense water status in the plants.

Synthesis and Crystal Structure of 2-Benzylamino-6-methyl-3-cyano-8- phenyl-5H-bispyrazolo[3,4-d,3',2'-b]pyrimidine

The title compound 2-benzylamino-6-methyl-3-cyano-8-phenyl-5H-bispyrazolo[3,4-d,3＇,2＇-b]pyrimidine crystallizes in orthorhombic, space group Pbca with a = 17.945（7）, b =10.862（4）, c = 19.481（7） A°, β = 90°, Z = 8, V = 1151.8（4） A°^3, Mr = 379.43, Dx = 1.327 g/cm^3,μ（MoKa） = 0.084 mm^-1, F（000） = 1584, the final R = 0.0513 and wR = 0.1128 for 2608 observed reflections （I 〉 2σ（I））. X-ray analysis reveals that the tricyclic portion of the molecule is effectively planar. In addition, there exist three intermolecular hydrogen bonds.

Increasing the Protein Amount of Chlorella vulgaris [Chlorphyta] Strains Isolated from Different Fresh Water Ponds

Chlorella vulgaris is a single-cell, spherical green algae and one of the microalgae on which many applied studies are conducted. In the present study, five strains displaying fast and efficient reproduction were chosen among 11 C. vulgaris strains isolated from different fresh water ponds and their cell numbers and the amount of chlorophyll a, protein, lipid, cellulose and carbohydrate were examined. The main goal of the study is to investigate increasing the biochemical contents especially the protein content of C. vulgaris strains in different mediums. In the present study, cell densities were determined through cell count for five days. In parallel with cell count, their chlorophyll a content was determined. The highest cell density was observed with C. vulgaris TOH （Tourism and Hotel Management Pond） strain as 5.5 × 104 h/mL, and the chlorophyll a content as 4.3 × 102 mg/m3. The highest intracellular protein amount was determined with C. vulgaris GUH （Gazi University Rectorship Pond） （0.061 g/100 mL） and the highest lipid amount was attained with C. vulgaris UIK （Ulus Construction Well） strain as 0.019 g/100 mL. The process of increasing the intracellular protein amount in C. vulgaris GUH strain was carried out in Prat, Yagojinski and Chlorella medium. The results indicated that Chlorella medium increased the intracellular protein amount.

Structural Features of the Vegetative Organs of <i>Allium praemixtum</i>Vved. in Different Ecological Conditions

Central Asia is one of the main centers of origin of bulbous geophytes, including the family Amaryllidaceae Juss. The anatomical features of many endemic, red-book bulbous geophytes are still poorly understood. The article presents the results of a structural study of the inflated part of the stem (fistular), stem and pedicels of an endemic A. praemixtum species grow in two different environmental conditions in the Aktau mountain Nurata Range (Uzbekistan, Province Navoi) and in the conditions of introduction in the Tashkent Botanical Garden (Uzbekistan, Tashkent city). Diagnostic signs of vegetative organs were revealed and various combinations of xeromorphic and mesomorphic signs were determined. Comparative anatomical study of vegetative organs of A. praemixtum is of great theoretical and practical importance in relation to taxonomy and ecology.

Some Biological Features of Tinca tinca Population in Asartepe Reservoir(Ankara)

Tinca tinca(L.,1758)grafted into Turkish inland waters in 1970 has shown constantly widening distribution since then.It is a locally consumed species with limited economic value.The elucidation of length-weigh relation,condition factor and biological features are of great importance in the determination of the effects of different habitats on particular species.T.tinca is regarded as an important species to be monitored among the fish taxa.This study was intended to study some biological aspects of T.tinca population in Asartepe Reservoir and to compare the results with the available information.The study has been carried out on 131 T.tinca species caught in Asartepe Reservoir between the periods of March 2015-February 2016.The morphometric and meristic properties and the ratio of some diagnostic features to the standard length were elucidated.The highest and the lowest variations observed among the morphometric features were in body length with 21.35%and eye diameter with 8.31%.The corresponding values for the meristic features were pectoral fin unbranched rays with 30.09%and number of vertebrae with 2.68%.The highest percentage relative to the standard length was found in predorsal distance with 56.95%.The age distribution of T.tinca population was between the ranges of 1-9 years of age.The average weight and length were found to be 520.58±207.84 g(103-1,302 g)and 308.68±41.38 mm(190-429 mm).The parameters of a and b of length and weight relation and the correlation coefficient were found to be 0.9692,3.0522 and 1.6807±0.1264(1.4199-2.0368),respectively.The results indicate that the growth level of T.tinca population is satisfactory.

Structural Features of the Vegetative Organs of <i>Allium kysylkumi</i>Kamelin. Growing in the Conditions of Kyzylkum

The article presents the results of studies of the anatomical structure of the vegetative organs of Allium kysylkumi of the Amaryllidaceae family, which grows in the conditions of Kyzylkum (Kokchatau outlier mountain). The characteristic diagnostic features have been determined: the outer membrane of epidermal cells is well developed by the outer folded cuticle, cutinized and more thickened;leaves are amphistomatic;stomata are the most submerged, anomocytic type;isolateral-palisade type of leaf mesophyll;the conducting bundles are closed, collateral and are arranged in two rows, of which the upper side is represented only by small bundles, in the lower row, 3 large bundles alternate with one small bundle;peduncle and peduncle, parenchymal-beam type of structure;the epidermis is single-row, folded cuticle with more thickened and cutinized;the crustal parenchyma is thin-walled, round-oval, chlorophyll-bearing;the presence of lactic acid is in the cow parenchyma;the primary cortex is separated from the central cylinder by a ring of sclerenchyma;the central cylinder is extensive;closed collateral-type conductive bundles. In the vegetative organs of Allium kysylkumi, especially in the leaf, xeromorphic characters are pronounced, which indicates the fitness of this species in natural habitat. The revealed structural diagnostic signs of vegetative organs are species-specific, and show the adaptation of this species to its natural habitat, these micromorphological signs are taxonomic significance for distinguishing and identifying Allium species.

Tip60 Tumor Suppressor Requires Its NLS Motif to Interact with Importin <i>α</i>

Tip60 is a specific member of MYST (Moz-Ybf2/Sas3-Sas2-Tip60) family of nuclear histone acetyltransferases (HAT). It is essential for cellular survival, differentiation, and metabolism. A putative canonical NLS motif between the chromo domain and the zinc finger of Tip60 was identified. Here we show evidence that Tip60 is associated with importin α as its substrate and transported from cytoplasm to the nucleus. Pull down assay revealed that Tip60 was physically associated with importin α both in vivo and in vitro. Confocal microscopic observation showed that Tip60 and importin α were co-localized with each other. The localization of Tip60 to the nuclear and its interaction with importin α was disrupted when its putative NLS motif for binding to importin α was mutated (219RKRK222 &#8594 219AAAA222). However, attachment of this putative NLS motif to a cytoplasmic protein (YAP 1-210 fragment) promoted its nuclear localization. Based on transient transfection, Tip60 NLS motif mutant showed a substantial reduction in self-acetylation, HAT activity, and apoptotic ability whereas wild type Tip60 did not show such reduction. Taken together, our results demonstrate that importin α transports Tip60 from the cytoplasm to the nucleus through binding to the putative NLS motif of Tip60 for its tumor suppressing function.

Potential of Binahong (<i>Anredera cordifolia</i>[Tenore] Steen) in Reducing TNF-<i>α</i>Expression on Regeneration of Pancreas <i>β</i>Cells on White Rats (<i>Rattus norvegicus</i>) Diabetes Mellitus Models

Diabetes mellitus (DM) is a metabolic disorder characterized by increased blood glucose levels (hyperglycemia) due to impaired insulin secretion and/or increased insulin resistance. Providing binahong leaf steeping (Anredera cordifolia [Tenore] Steen) is one alternative treatment for people with diabetes mellitus using medicinal plants. This research was conducted to find out the right concentration by administering binahong leaf steeping to reduce hyperglycemia in STZ induced rat and to analyze administration of binahong leaf steeping in reducing TNF-α expression in pancreatic β cell regeneration. This study used 25 white rats (Rattus novergicus) which were divided into 5 groups: group 1, negative control (K-), group 2, positive control (K+), groups 3, 4, and 5. With STZ induction, each is given a dose of binahong leaf steeping, 50.4 mg/180ml of water;100.8 mg/180ml of water and 151.2 mg/180ml of water for 14 days. The results showed that the administration of binahong leaf steeping to STZ induced rat could reduce glucose rats in a row by 130.00, respectively;117,23 and 112,45 and steeping binahong leaves (Anredera cordifolia [Ten] S) significantly influence the reduction in TNF-α expression in pancreatic β cell regeneration. Thus, this study can be concluded that the right dose to reduce hyperglycemic and regeneration of pancreatic β cells produced by STZ induced rats is 151.2 mg/180ml of water and statistically influences in reducing TNF-α expression in pancreatic β cell regeneration.

Taxonomic and community composition of epigeal arthropods in monoculture and mixed tree species plantations in a deciduous forest of Ghana

Tropical forests provide several ecosystem services and functions and support approximately two-thirds of the world’s biodiversity but are seriously threatened by deforestation.Approaches to counteract this menace have revolved around aff orestation with several or a single tree species.We thus investigated how plantation forests with either a single or several tree species infl uenced arthropod taxonomic and community composition using pitfall traps to sample selected groups of epigeal arthropods(Araneae,Coleoptera,Orthoptera and Hymenoptera)and with environmental variables assessed simultaneously.Our results revealed 54 taxonomic groups with signifi cantly higher taxonomic richness,activity density,and diversity in the mixed stands than in the monoculture stands.The significant differences in community composition were mainly driven by families including Lycosidae,Formicidae,Staphylinidae,Scotylidae,Hydrophilidae,Gryllidae and Scarabaeidae and were explained by distinct habitat characteristics(canopy openness,litter depth,deadwood volume,and tree height).While the diverse tree communities and heterogeneous vegetation structure off ered food and habitat resources for diverse arthropod groups,the allelopathic nature coupled with homogenous stand characteristics of the Tectona grandis stands in the monoculture suppressed the growth of understorey vegetation that could otherwise serve as food and habitat resources for arthropods,which might have led to limited activities and diversity of arthropods in the monoculture plantation stands.The fi ndings thus highlight the need to promote mixed tree plantations in degraded tropical areas,especially when restoring biodiversity is the prime management focus.

<i>Fasciola hepatica</i>and Associated Parasite, <i>Dicrocoelium dendriticum</i>in Slaughter Houses in Anyigba, Kogi State, Nigeria

Fasciola hepatica is a parasite of clinical and veterinary importance which causes fascioliasis that leads to reduction in milk and meat production. Bile samples were centrifuged at 1500 rpm for ten (10) minutes in a centrifuge machine and viewed microscopically to check for F. hepatica eggs. A total of 300 bile samples of cattle which included 155 males and 145 females were collected from the abattoir. Results were analyzed using chi-square (p > 0.05). The prevalence of F. gigantica and Dicrocoelium dentriticum is 33.0% (99) and 39.0% (117) respectively. Age prevalence of F. hepatica revealed that 0 - 2 years (33.7%, 29 cattle) were more infected than 2 - 4 years (32.7%, 70 cattle) while for D. dentriticum age 2 - 4 years were more infected than 0 - 2 years with prevalence of 40.2% (86) and 36.0% (31) respectively. No significant difference (P > 0.05) existed in prevalence in ages of the cattle. Out of the 300 bile samples examined, 22.3% (67 cattle) were co-infected with F. hepatica and D. dendriticum. Males were more co-infected than females having a prevalence of 24.5% (38 cattle) and 20.0% (29 cattle) respectively. Based on the age, samples of age 0 - 2 years were more co-infected than those of age 2 - 4 years with a prevalence of 23.3% (20 cattle) and 22.0% (47 cattle) respectively. The findings of this present study revealed that efforts to alleviate problems of animal health and productivity are yet to make any significant impact as this poses threat on human health. Investigation on the pattern of infections in cattle slaughtered should be strengthened.

Expression,Purification,Crystallization and Preliminary Crystallographic Analysis of Nucleoside Diphosphate Kinase(NDK)from Aspergillus Flavus

Aspergillus flavus causes serious disease on important agriculture crops, and its secondary metabolic products-aflatoxins are most potent toxin and carcinogen for animal and human, Structural and functional studies ofA. flavus proteins may provide insights into the identification of potential therapeutic targets and prevention of damage caused by A. flavus. Here, we report the expression, purification, crystallization and preliminary crystallographic analysis of NDK protein from A. flavus. The NDK protein was expressed in E. coli and purified by using a series of chromatographic methods to 〉98% purity. The recombinant protein was crystallized and the crystals diffracted to 2.4 A^° resolution. The crystal of NDK is in space group of C121 with a = 190.84, b = 169.47, c = 146.94 A^°. Preliminary data analysis indicated that the NDK molecule assembles into a multimer in the asymmetric unit.

Phosphorylation on TRPV4 Serine Residue 824 Enhances Its Association with PGM1

The TRPV4 cation channel is expressed in a broad range of tissues and participates in the generation of a Ca2+ signal and/or depolarization of membrane potential. Here, human phosphoglucomutase- 1 (PGM1), an enzyme that converts glucose-6 phosphate to glucose-1 phosphate in the glycolysis pathway, as the first auxiliary protein of TRPV4 Ca2+ channels, is identified with yeast two hybrid system, coimmunoprecipitation, confocal microscopy, and GST pull-down assays. TRPV4 forms a complex with PGM1 through its C-terminal cytoplasmic domain. Because it is demonstrated that TRPV4 serine residue 824 (S824) is phosphorylated by serum/glucocorticoid regulated kinase 1, we elucidate the effect of TRPV4 S824 phosphorylation on TRPV association with PGM1. Even an inactivated mutant version of TRPV4, S824A, exhibited a decreased ability to bind PGM1, an activated phosphomimetic mutant version of TRPV4, S824D, exhibited enhanced binding to PGM1. Thus, formation of the TRPV4/PGM1 complex and localization of this complex to the plasma membrane appear to be regulated by the phosphorylation status of residue S824 in TRPV4. The newly identified interactor of TRPV4 may help the molecular pathways modulating transport activity or glucose metabolism, respectively.

Dietary Coleus amboinicus Lour. decreases ruminal methanogenesis and biohydrogenation, and improves meat quality and fatty acid composition in longissimus thoracis muscle of lambs

Background:Methane production and fatty acids(FA)biohydrogenation in the rumen are two main constraints in ruminant production causing environmental burden and reducing food product quality.Rumen functions can be modulated by the biologically active compounds(BACs)of plant origins as shown in several studies e.g.reduction in methane emission,modulation of FA composition with positive impact on the ruminant products.Coleus amboinicus Lour.(CAL)contains high concentration of polyphenols that may potentially reduce methane production and modulate ruminal biohydrogenation of unsaturated FA.This study aimed to investigate the effect of BAC of Coleus amboinicus Lour.(CAL)fed to growing lambs on ruminal methane production,biohydrogenation of unsaturated FA and meat characteristics.In this study,the in vitro experiment aiming at determining the most effective CAL dose for in vivo experiments was followed by two in vivo experiments in rumen-cannulated rams and growing lambs.Experiment 1(RUSITEC)comprised of control and three experimental diets differing in CAL content(10%,15%,and 20%of the total diet).The two in vivo experiments were conducted on six growing,rumen-cannulated lambs(Exp.2)and 16 growing lambs(Exp.3).Animals were assigned into the control(CON)and experimental(20%of CAL)groups.Several parameters were examined in vitro(pH,ammonia and VFA concentrations,protozoa,methanogens and select bacteria populations)and in vivo(methane production,digestibility,ruminal microorganism populations,meat quality,fatty acids profiles in rumen fluid and meat,transcript expression of 5 genes in meat).Results:CAL lowered in vitro methane production by 51%.In the in vivo Exp.3,CAL decreased methane production by 20%compared with the CON group,which corresponded to reduction of total methanogen counts by up to 28%in all experiments,notably Methanobacteriales.In Exp.3,CAL increased or tended to increase populations of some rumen bacteria(Ruminococcus albus,Megasphaera elsdenii,Butyrivibrio proteoclasticus,and Butyrivibrio fibrisolvens).Dietary CAL suppressed the Holotricha population,but increased or tended to increase Entodiniomorpha population in vivo.An increase in the polyunsaturated fatty acid(PUFA)proportion in the rumen of lambs was noted in response to the CAL diet,which was mainly attributable to the increase in C18:3 cis-9 cis-12cis-15(LNA)proportion.CAL reduced the mRNA expression of four out of five genes investigated in meat(fatty acid synthase,stearoyl-CoA desaturase,lipoprotein lipase,and fatty acid desaturase 1).Conclusions:Summarizing,polyphenols of CAL origin(20%in diet)mitigated ruminal methane production by inhibiting the methanogen communities.CAL supplementation also improved ruminal environment by modulating ruminal bacteria involved in fermentation and biohydrogenation of FA.Besides,CAL elevated the LNA concentration,which improved meat quality through increased deposition of n-3 PUFA.Highlight·Coleus amboinicus Lour.(CAL)into sheep diet decreased CH4emission.·CAL did not reduce nutrient digestibility,but inhibited the methanogen community.·CAL increased ruminal propionate proportion and decreased acetate/propionate ratio.·CAL elevated n-3 fatty acid concentration in ruminal fluid and meat.·Supplementation of CAL improved some meat quality traits.