Research on the Significance of Incremental Utilization of Natural Resources Based on "Fenlong Science of Natural Resources"

The basic principle of Fenlong technology is to make full use of the cultivated layer and the below soil resources and natural precipitation. It can increase the amount of loosen soil by 1-2 times, water and oxygen by 1 time, and available nutrients by 10%-30%, surface air humidity by more than 10%, crop net photosynthetic efficiency by 10%-30%, biomass by 20%-30%, crop yield by 10%-50%, and crop quality by 5%, and decrease salt content by 20%-40% and the emission of methane and other gases by 10%. In this study, it is firstly proposed to establish a new research field——"Fenlong science of natural resources"(referred to as "Fenlong science"), and the research contents include Fenlong-based sciences of modern agricultural machinery and equipment for full-layer ploughing and bottom ploughing, tillage, crop cultivation, utilization of water resources (natural precipitation, farmland water conservancy, groundwater resources, etc.), conversion and utilization of saline-alkali land, arable degraded grassland reconstruction, ecological reconstruction of desertification land, ecological environment, climate change, economic and social development. It clarifies theory and enriches technology, and provides a major platform for carrying global population developing from more than 7 billion to 10 billion and helping people and nature to mutually benefit.

Rapid gene expression change in a novel synthesized allopolyploid population of cultivated peanut×Arachis doigoi cross by cDNA-SCoT and HFO-TAG technique

AIIopolyploidy has played an important role in plant evolution and heterosis. Recent studies indicate that the process of wide hybridization and （or） polyploidization may induce rapid and extensive genetic and epigenetic changes in some plant species. To better understand the allopolyploidy evolutionism and the genetic mechanism of Arachis interspecific hybridization, this study was conducted to monitor the gene expression variation by cDNA start codon targeted polymorphism （cDNA-SCoT） and cDNA high-frequency oligonucleotide-targeting active gene （cDNA-HFO-TAG） techniques, from the hybrids （F1） and newly synthesized allopolyploid generations （S0-$3） between tetraploid cultivated peanut Zhongkaihua 4 with diploid wild one Arachis doigoi. Rapid and considerable gene expression variations began as early as in the FI hybrid or immediately after chromosome doubling. Three types of gene expression changes were observed, including complete silence （gene from progenitors was not expressed in all progenies）, incomplete silence （gene expressed only in some progenies） and new genes activation. Those silent genes mainly involved in RNA transcription, metabolism, disease resistance, signal transduction and unknown functions. The activated genes with known function were almost retroelements by cDNA-SCoT technique and all metabolisms by cDNA-HFO-TAG. These findings indicated that interspecific hybridization and ploidy change affected gene expression via genetic and epigenetic alterations immediately upon allopolyploid formation, and some obtained transcripts derived fragments （TDFs） probably could be used in the research of molecular mechanism of Arachis allopolyploidization which contribute to thwe genetic diploidization of newly formed allopolyploids. Our research is valuable for understanding of peanut evolution and improving the utilization of putative and beneficial genes from the wild peanut.

Phylogenetic Relationships in Genus Arachis Based on SSR and AFLP Markers

Fourteen wild species of different sections in the genus Arachis and 24 accessions of the AABB allotetraploid A. hypogaea （cultivated peanut） from several countries which belong to different botanical varieties, were analyzed by SSR and AFLP marker systems. The assay-units per system needed to distinguish among all the tested accessions were at least five for SSR or two for AFLP. The genetic distance detected by the SSR markers ranged from 0.09 to 0.95, and the mean was 0.73; and the genetic distance detected by the AFLP markers ranged from 0.01 to 0.79 with an average of 0.42. All the tested peanut SSR primer pairs were multilocus ones, and the amplified fragments per SSR marker in each peanut genome ranged from 2 to 15 with the mean of 4.77. The peanut cultivars were closely related to each other, and shared a large numbers of SSR and AFLP fragments. In contrast, the species in the genus Arachis shared few fragments. The results indicated that the cultivated peanut （A. hypogaea L.） varieties could be partitioned into two main groups and four subgroups at the molecular level, and that A. duranensis is one of the wild ancestors of A. hypogaea. The lowest genetic variation was detected between A. cardenasii and A. batizocoi, and the highest was detected between A. pintoi and the species in the section Arachis. The relationships among the botanical varieties in the cultivated peanut （A. hypogaea L.） and among wild species accessions in section Arachis and those in other sections in the genus Arachis were discussed.

A novel long non-coding RNA, DIR, increases drought tolerance in cassava by modifying stress-related gene expression

Cassava is an important tropical cash crop. Severe drought stresses affect cassava productivity and quality, and cause great economic losses in agricultural production. Enhancing the drought tolerance of cassava can effectively improve its yield. Long non-coding RNAs(lncRNAs) are present in a wide variety of eukaryotes. Recently, increasing evidence has shown that lncRNAs play a critical role in the responses to abiotic stresses. However, the function of cassava lncRNAs in the drought response remains largely unknown. In this study, we identified a novel lncRNA, DROUGHT-INDUCED INTERGENIC lncRNA(DIR). Gene expression analysis showed that DIR was significantly induced by drought stress treatment, but did not respond to abscisic acid(ABA) or jasmonic acid(JA) treatments. In addition, overexpression of the DIR gene enhanced proline accumulation and drought tolerance in transgenic cassava. RNA-seq analysis revealed that DIR preferentially affected drought-related genes that were linked to transcription and metabolism. Moreover, RNA pull-down mass spectrometry analysis showed that DIR interacted with 325 proteins. A protein–protein interaction(PPI)analysis found a marked enrichment in proteins associated with the mRNA export and protein quality control pathways.Collectively, these results suggest that DIR and its interacting proteins that regulate mRNA or protein metabolism are involved in mediating the drought stress response. Thus, regulating DIR expression has potential for improving cassava yield under drought conditions.

Effects on Agronomic Traits,Yield and Benefit of One-year Triple Cropping "Maize-soybean" Strip Intercropping System Under Reduced Fertilizer Application

[Objectives] This study was conducted to investigate the effects on agronomic traits,yield and benefit of one-year triple cropping " maize-soybean" strip intercropping system under reduced fertilizer application. [Methods]A single factor randomized block design was used,and wide and narrow strip intercropping was adopted. Three different fertilization treatments,namely fertilizers for conventional field fertilization( A1),80% A1( A2) and 60% A1( A3) were applied to the field production. The fertilizing sites were about 25 cm away from maize and repeated three times. [Results]Compared with A1,the change laws of the agronomic traits and yield in the A2 and A3 intercropping systems were described as below. Agronomic traits: The plant height became shorter gradually for the spring soybean,but increased gradually for the summer soybean,the bottom pod height gradually reduced,and the node number,pods per plant and seeds per plant increased;and the plant height,ear length,rows per ear,seeds per row and 1 000-seed weight of the spring maize decreased. Yield: The yield of maize as the main crop decreased but not significant,by 3. 20% and 3. 99%,respectively in A2 and A3,both smaller than 5. 00%;the yield of the spring soybean significantly increased by 9. 70% and 11. 84%,respectively;the yield of the summer soybean increased by 5. 18% and 8. 98%,respectively;and the total yield increased by 0. 20% and 0. 92%,respectively. Benefit: The total output value increased by 2. 97 % and 4. 91 %,respectively,and the total benefit increased by 6. 39% and 11. 22%,respectively. [Conclusions] Under a 40% reduction in fertilizer applied in the field,the one-year triple cropping " maize-soybean" strip intercropping system still met the multi-target requirements of increasing grain production and economic benefits,and thus can be promoted and applied in Guangxi.

The Salt Reduction and Yield Increase Effects of Smashing Ridging Transforming Saline-alkali Land and Its Development Prospects

Using smashing ridging tillage machine and smashing ridging technology invented by the authors, transformation test of saline-alkali land by smashing ridging was conducted respectively in Xinjiang and Shaanxi during 2015 -2016. The results showed, in severe saline-alkali soil of Xin-jiang ,after growing cotton by smashing ridging, total salt in soil decreased by 31.31 %, cotton production increased by 48.80%, and salinity level declined from severe to moderate; in mild saline-alkali soil of Shaanxi, after growing summer corn by smashing ridging, total salt in soil decreased by 42.37%, corn yield increased by 34.83%, salinity degree changed from mild desalination to normal farmland ; in Ningxia, Inner Mongolia 7 Gansu ,Jilin, Henan, Hebei, and so on, smashing ridging tillage practice in different types of saline-alkali land was conducted ; according to the salt reduction and yield increase effects of saline-alkali land after smashing ridging, the development prospect of smashing ridging technique in improvement and application of saline-alkali land was proposed.

Telomere-to-telomere assembly of cassava genome reveals the evolution of cassava and divergence of allelic expression

Cassava is a crucial crop that makes a significant contribution to ensuring human food security.However,high-quality telomere-totelomere cassava genomes have not been available up to now,which has restricted the progress of haploid molecular breeding for cassava.In this study,we constructed two nearly complete haploid resolved genomes and an integrated,telomere-to-telomere gap-free reference genome of an excellent cassava variety,‘Xinxuan 048’,thereby providing a new high-quality genomic resource.Furthermore,the evolutionary history of several species within the Euphorbiaceae family was revealed.Through comparative analysis of haploid genomes,it was found that two haploid genomes had extensive differences in linear structure,transcriptome features,and epigenetic characteristics.Genes located within the highly divergent regions and differentially expressed alleles are enriched in the functions of auxin response and the starch synthesis pathway.The high heterozygosity of cassava‘Xinxuan 048’leads to rapid trait segregation in the first selfed generation.This study provides a theoretical basis and genomic resource for molecular breeding of cassava haploids.

Effects of Seed Soaking with Paclobutrazol on Tillering and Physio-logical Characteristics of Sugarcane Seedlings

In order to study the effects of seed soaking with Paclobutrazol( PP333) on the tillering and physiological characteristics of sugarcane seedlings,sugar cane variety ROC22 was used as experimental material. The effects of seed soaking with different concentrations of PP333 were studied. The tiller number and growth of sugarcane were surveyed at the 6-leaf stage,the physiological indicators such as chlorophyll content,soluble protein content,proline content and peroxidase activity were measured at the 2-leaf,4-leaf,and 6-leaf stages. The results showed that the seed soaking with PP333 can effectively improve the tillering of sugarcane seedlings. The suitable concentration for the tillering of sugarcane and the growth and development of sugarcane was of 50 mg/L; the seed soaking with PP333 significantly increased the chlorophyll,soluble protein,proline,and peroxidase activity of leaves of sugarcane seedlings; the content of chlorophyll and soluble protein in leaves of sugarcane seedlings treated with 90 mg/L PP333 was the highest,and the proline content and peroxidase activity of sugarcane treated with 50 mg/L PP333 were the highest.

Effects of Application of Cassava Alcohol Anaerobic Fermentation Liquid and Mulching of Film on Overwintering and Next Year's Growth and Yield of Banana

The effects of application of cassava alcohol anaerobic fermentation liquid and mulching of film on overwintering and next year's growth and yield of banana were investigated in this study.The results showed that the application of cassava alcohol anaerobic fermentation liquid with COD concentration of 1 200-2 000 mg/L(900 m^3/hm^2) and mulching of film significantly increased the soil organic matter and available potassium contents in banana orchard,improved the cold resistance of banana seedlings,increased the pseudostem height,pseudostem circumference,green leaf number and chlorophyll content of next year's banana seedlings,brought the flower bud emergence period and harvest period forward,and significantly increased the yield per plant.

"Fenlong Law" and Its Scientific Significance

Based on application of " Fenlong tillage technology" in 40 kinds of crops in 26 provinces of China,and saline alkali land and degraded grassland of 10 provinces in the past 10 years, " Fenlong law" that Fenlong depth of various land resources suitable for cultivation in reasonable depth interval and utilization degree of " natural resources" are proportional to increase in agricultural output,ecological environment improvement and increase in economic output is discussed in this paper. Moreover,its formula,8 " effect laws" and their scientific significance are given. " Full-layer tillage" or " bottom-layer tillage" of Fenlong " super deep ploughing,deep loosening and not disturbing soil layer" is expounded,and the way of multiple incremental utilization of " natural resources" is created,which could be applied in various land resources suitable for cultivation,and realizes deep utilization of natural resources in a " big explosion". Additionally,China has 147 million ha of cultivated land,saline alkali land and degraded grassland,and 400 million people can be supported by the newly increased grain and meat,etc. by Fenlong,and 88 billion m^3 of land water resources is added. " Fenlong law" is proposed based on Fenlong phenomenon,and it is conducive to deep enjoyment and protection of natural resources by human beings,and harmonious development between man and nature.

Molecular characterization, expression and function analysis of eukaryotic translation initiation factor(eIF1A) in Mangifera indica

Eukaryotic translation initiation factor 1A(eIF1A)functions as an important regulatory factor of protein synthesis and plays a crucial role in responses to abiotic stresses in plants.However,little is known about the eIF1A gene involved in fruit development and stress response of mango.In this study,the MieIF1A-b gene was isolated from Mangifera indica,and contains a 435-bp open reading frame,which encodes a putative protein of 144 amino acids(GenBank accession number:KP676599).The predicted MieIF1A-b protein had a molecular weight of 16.39 kDa with a pI of 4.6.Sequence homology analysis showed that MieIF1A-b shared high homology with Elaeis guineensis,Manihot esculenta,and Populus trichocarpa,with 96 and 95%identity,respectively.Quantitative reverse transcriptative PCR(qRT-PCR)analyses indicated that MieIF1A-b was expressed in all tested tissues,and had the highest expression level in fruit 80 d after flowering.The expression of MieIF1A-b was obviously regulated by NaCl and H2O2 treatments in leaves.Functional analysis indicated that the overexpression of MieIF1A-b in transgenic Arabidopsis thaliana enhanced the growth,phenotype and salinity tolerance compared with wild-type(WT)plants.The results indicated that MieIF1A-b may be correlated with the control of fruit development and salt adaptation,and it was a candidate gene for abiotic stress in mango.

Roles of Fenlong Technology in the Increase of Grain Yield and Construction of a Huge "Underground Reservoir"

Fenlong farming technology was introduced. It has characteristics of very deep tillage and evenly smashing soil,and can evenly smash soil without disturbing soil layers to complete the task of soil preparation. It can be widely used in cultivated land,saline-alkali land,lime concretion black soil,degraded grassland,etc. After the application of the technology in 34 crops in 24 provinces,it can increase yield by 10%-50% and improve quality by above 5%. Fenlong technology can increase soil reservoir capacity,enhance the infiltration rate of rainwater in soil and make " surplus" effect obvious. If the country plans and promotes the technology in 160 million hm^2 of land( including 67 million hm^2 of cultivated land,20 million hm^2 of saline-alkali land,6. 7 million hm^2 of marginal land and 67 million hm^2 of degraded grassland),the total volume of loosened soil in arable land will increase from 198. 1 billion to 840. 0 billion m^3,and the capacity of a " underground reservoir" will increase by 675 million m^3,while natural precipitation reserves will increase by 162 billion m^3. It can effectively solve food security problems and water resource problems such as drought,floods,and industrial,agricultural and domestic water shortages,as well as serious disaster hazards caused by groundwater that has been evacuated in China.

“Physical Fertility” of Fenlong and Its Yield and Benefit Increase

Core theory and acting force("physical fertility") of Fenlong are firstly proposed,and "physical fertility of Fenlong" is relative to the fertility of chemical fertilizer of increasing crop yield. Basic composition of "physical fertility of Fenlong" and its effect of increasing production and improving quality of various agricultural crops are clarified. It makes crop yield increase by 15%-30% without fertilization and 15%-50% by not increasing fertilization amount and irrigation water consumption. It is proposed that "physical fertility of Fenlong" could be sufficiently excavated in the new times of green development. Fenlong has a key significance for rationally reducing chemical fertilizer,soil,water and air pollution,agricultural cost,improving the quality of agricultural products and human health level.

Preliminary Explanation on "Natural Activation Theory" Based on "Fenlong"

Based on action mechanism of "Fenlong technology" with the maximum activation potential of global natural resources,"natural resources activation theory"("activation theory") is proposed firstly.Its connotation contains two parts.One is directly activating some natural resources in land,such as unutilized plough bottom of existing cultivated land and soil resources below it,saline-alkali land,degraded grassland,ecological reconstruction of desertification land,inter-row of fruit trees,lawn of sponge city,etc.The other is indirectly activating some natural resources in ground space,such as natural precipitation,solar energy,oxygen,terrestrial eco-environment,soil resources,river waters and even climate change factors.It could release and generate huge physical endogenous force and cyclic force,and increase food source and available water resources,which is favorable for human survival,nature's own ecological balance,harmonious coexistence and balance between man and nature,without harming nature itself.Moreover,its supporting theoretical system is explained from five aspects.It is pointed that the "activation theory" is deep derivation of "Fenlong technology" and "Fenlong science".By sufficiently playing the potential of natural resources,it could reach human-dominated comprehensive balance and coordinated development of air,sunshine,water,soil,nutrient,microorganism,plant,livestock and poultry,marine organism in the nature.The "activation theory" is concrete embodiment of respecting,changing and using the nature,and new channel of realizing sublimation of "life community of human and nature" and benefiting mankind.

iTRAQ proteomics reveals the regulatory response to Xanthomonas campestris pv.vesicatoria in resistant vs.susceptible pepper genotypes

Bacterial spot(BS)is a severe bacterial disease induced by Xanthomonas campestris pv.vesicatoria(Xcv),a pathogen that causes serious damage to pepper growth and yield.It is therefore important to study the mechanisms of pepper resistance to Xcv and to breed and promote Xcvresistant pepper varieties.However,studies of the responses to Xcv infection in peppers at the protein level are limited.Here,we examined Xcv-induced proteomic changes in leaves of the BS susceptible bell pepper ECW and the resistant bell pepper VI037601 using the isobaric tags for relative and absolute quantitation(iTRAQ)-based protein labeling technology.A total of 6,120 distinct proteins were identified,and there were 1,289 significantly differentially accumulated proteins(DAPs)in ECW and VI037601 leaves after Xcv inoculation.Among these,339(250up-and 89 down-regulated)and 479(300 up-and 179 down-regulated)DAPs were specifically identified in ECW and VI037601,respectively,with 459(364 up-and 95 down-regulated)similarly expressed DAPs being shared by ECW and VI037601.Based on bioinformatics analysis,many defense-associated proteins were identified as up-regulated in ECW and VI037601,especially the proteins involved in plant-pathogen interaction,phenylpropanoid biosynthesis,protein processing in the endoplasmic reticulum,and MAPK signaling pathway-plant.Moreover,we evaluated transcript levels of six differentially expressed genes from the iTRAQ results by q RT-PCR.The analysis revealed transcriptional changes that were consistent with the changes at the protein level.This study will provide a valuable resource for understanding the molecular basis of pepper resistance to Xcv infection and for improving the disease resistance of pepper cultivars.

Development of Molecular Marker Linked to Cf-10 Gene Using SSR and AFLP Method in Tomato

The leaf mould resistance gene Cf-10 on tomato confered resistant or immune to all prevalent physiological races of Cladosporium fulvum presented in three northeastern provinces of China in inoculation test. In order to better utilize Cf-10 gene in a marker-assisted selection program and to permit the pyramiding of one or several resistance genes in a cultivar, tightly linked SSR and AFLP markers were obtained by the bulked segregant analysis method. One SSR marker and three AFLP markers were identified linked to Cf-10 gene, with the distance of 9.73, 5.8, 8.5, and 10.6 cM, respectively. These markers will facilitate the selection of resistant tomato germplasm containing Cf-10 gene.

Tillage Effect and Development Prospect of Fenlong Farming Tools with"Drill"

In this paper,farming and crop cultivation effects of farming tools with"drill"which are invented independently in China are clarified firstly,containing horizontal cutting of soil,super deep ploughing,deep loosening and not disturbing soil layer,granular soil,soil not easy to bond and harden,and one-time land preparation.It is a significant path to deeply develop and use the"five natural resources"not fully utilized by human beings(soil resources at plough bottom and below,saline-alkali land,natural rainfall,solar energy,oxygen)and realize"mitigation"of five natural disasters(flood,drought,high temperature,low temperature and climate warming).In the past 10 years,the farming tools have been applied in 40 kinds of crops in 26 provinces of China.Compared with vertical breaking of soil by traditional"plow",the amount of loose soil,water storage and dissolved oxygen in cultivated land increased by more than one time,and yield increased by 10%-50%.They have been applied in transformation of saline-alkali land in 10 provinces,and yield increased by 30%-150%.After Fenlong treatment for 1 and 2 years,biomass of sugarcane increased by 68.9%and 50.1%respectively.Net photosynthetic rates of Gramineous crops were improved by 6.82%-11.94%,while net photosynthetic rates of Legume crops were improved by 20.32%-32.08%.After"spiral drill","vertical two-knife drill","vertical three-knife drill"and other hollow series of Fenlong farming toolss were invented newly.They were the most advanced farming tools with large soil discharge and small resistance,and could be equipped in large-,medium-and small-size modern Fenlong agricultural machinery as key technology,thereby setting up a new"standard stalk"for world agricultural reform and bringing benefit to mankind.

Fenlong Tillage System by the Theory of “Natural Forces” and Possibility of “Fenlong Agriculture” Establishment

"Natural forces" ——core theory of Fenlong technique is explored in this paper. We break through key core technique of farming and agriculture,and invent new method of Fenlong tillage. Broad-spectrum Fenlong tillage technique system based on "full-layer and bottom-layer superdeep tillage without disturbance of soil layer" is established,which provides the possibility for establishing "modern Fenlong agriculture". When applied in 35 kinds of crops in 24 provinces of China,yield could be increased by 10%-50%,and even 1 times. In the transformation of severe saline-alkali land,corn yield could increase by 73. 0%,and Na,Cl and Mg ions in grain decrease by 20. 81%,1. 47%,and 9. 36% respectively. The newly developed "scarification tillage( reclamation) in the bottom area" could cover "bottom tillage" of dryland,paddy field,degraded grassland,perennial sugarcane,forest and fruit land,traditional Chinese medicine land,and ecological reconstruction of desertification land. It is point out that if Fenlong technique is used in 666 666. 67 km2 of farmland,200 000 km2 of saline-alkali land,666 666. 67 km2 of degraded grassland,and fisheries in rivers and offshore waters are used,trillion yuan of Fenlong economy could be formed,and the nationals will walk towards a healthier new era.Fenlong technique involves agriculture,forestry,water,grass,environment and other aspects,and its global promotion could improve eco-environment and change world development pattern.

Effect of Gibberellin,Light and Genotype on Somatic Embryogenesis and Plantlet Regeneration of Peanut

[ Objective] Peanut in vitro regeneration system was optimized to provide technical support for its genetic transformation and mutagenesis. [ Method ] The effect of gibberellin, light and genotype on somatic embryogenesis and plantiet regeneration of peanut was studied using Guihua peanut cuhivar as materials, leallets as explants, MS ＋ 10 mg/L 2, 4-D as somatic embryo induction medium, and MS ＋3 mg/L 6-BA ＋0. 8 mg/L NAA ＋ （0 - 15 mg/L） GA3 as plantlet induction me- dium. [Result]The somatic embryo induction rate of five peanut varieties under light condition of light： dark = 14 h： 10 h was significantly higher compared with that under dark condition by 7, 5 - 37.5 percent points. Among different peanut varieties, Guihua 26 represented the highest somatic embryo induction rate of 62. 8%, while Guihua 833 represented the lowest somatic embryo induction rate of 21.7%. Adding 5 - 15 mg/LGA3 in plantlet induction medium was conducive to improving the induction rate of plandets derived from somatic embryos. With addition of 5 mg/L GA3, Guihua 26 and Guihna 771 represented the highest plantlet induction rate of 42.8% and 35.3%, respectively; with addition of 15 mg/L GA3, Guihua 836, Guihua 1026 and Guihna 833 represented the highest plantlet in- duction rate of 38.7%, 33.3% and 26.4L%, respectively. Among different combinations of peanut variety and GA3 concentration, plantlet induction rate reached the highest in the combination Guihua 26 ＋ 5 mg/L CA.3 arid reached the lowest in the combination Guihua 836 ＋ 15 mg/L GA3. [ Conclusion] Appropriate light conditions are conducive to peanut somatic embryogenesis in vitro. Adding GA3 in plantlet induction medium is conducive to promoting plantlet regeneration. Guihua 26 and Guihua 836 are the best peanut varieties among the experimental genotypes for somatic embryogenesis and plant regeneration.

Scientific Value of the Fenlong Tillage Erupting Super "Natural Forces" and Its Global Application

The concept of " natural forces" is proposed in farming field for the first time,and the core theory of Fenlong tillage is illustrated. This tillage method provides " super plough layer" and " super groundwater reservoir" by the machine,including six vertical spiral drills,can smash the soil vertically,without disorganizing soil layer. And this tillage method releases five " natural forces" with infinite space and time,including natural soil activation forces,natural fertility forces,natural water control forces,natural environment improvement forces,and natural river driving forces by activating various land resources. Fenlong tillage can significantly increase the source of high-quality food for human,and solve the problem of natural disasters by natural forces curing itself. It is proposed that the implementation of the " six key projects" by the " natural forces" of Fenlong tillage can solve the deep problems in China. The researchers suggest establishing Fenlong tillage great science engineering which could solve the problem of harmonious symbiosis between man and nature. The present results indicate that Fenlong tillage erupts super natural forces with infinite space and time,which can be used as a deep tillage practice to maintain the natural and environmental beauty of the earth forever.