Electroacupuncture improves myocardial fibrosis in heart failure rats by attenuating ECM collagen deposition through modulation of TGF-β1/Smads signaling pathway

Background: To explore the effects of electroacupuncture on cardiac function and myocardial fibrosis in rat models of heart failure, and to elucidate the underlying mechanism of electroacupuncture in heart failure treatment. Methods: Healthy male Sprague-Dawley rats were allocated into three groups: Sham group, Model group, and electroacupuncture (Model + EA) group, with each group comprising 8 rats. The model underwent a procedure involving the ligation of the left anterior descending coronary artery to induce a model of heart failure. The Model + EA group was used for 7 consecutive days for electroacupuncture of bilateral Shenmen (HT7) and Tongli (HT5), once a day for 30 min each time. Left ventricular parameters in rats were assessed using a small-animal ultrasound machine to analyze changes in left ventricular end-diastolic volume, left ventricular end-systolic volume, left ventricular ejection fraction, and left ventricular fractional shortening. Serum interleukin-1β (IL-1β), cardiac troponin (cTn), and N-terminal brain natriuretic peptide precursor levels were measured using ELISA. Histopathological changes in rat myocardium were observed through HE staining, while collagen deposition in rat myocardial tissue was assessed using the Masson staining method. Picro sirius red staining, immunohistochemical staining, and RT-qPCR were utilized to distinguish between the various types of collagen deposition. The expression level of TGF-β1 and SMAD2/3/4/7 mRNA in rat myocardial tissues was determined using RT-qPCR. Additionally, western blot analysis was conducted to assess the protein expression levels of TGF-β1, SMAD3/7, and p-SMAD3 in rat myocardial tissues. Results: Compared with the Sham group, the left ventricular ejection fraction and left ventricular fractional shortening values of the Model group were significantly decreased (P < 0.01);the left ventricular end-diastolic volume and left ventricular end-systolic volume values were remarkably increased (P < 0.01);serum N-terminal brain natriuretic peptide precursor content was increased (P < 0.01);serum IL-1β and cTn levels were increased (P < 0.01);myocardial collagen volume fraction were increased (P < 0.01);and those of the expression of TGF-β1 and SMAD2/3/4 mRNA was increased (P < 0.01);the expression of SMAD7 mRNA was decreased (P < 0.01);the protein expression levels of TGF-β1, SMAD3, and p-Smad3 were increased (P < 0.01);the protein expression level of SMAD7 was decreased (P < 0.01) in the Model group. Compared to the Model group, the expression levels of the proteins TGF-β1, SMAD3, and p-Smad3 in myocardial tissue were found to be decreased (P < 0.01), and the expression level of the protein SMAD7 was found to be increased (P < 0.01) in the Model + EA group;the collagen volume fraction and deposition of type Ⅰ /Ⅲ collagen were decreased (P < 0.01) in the Model + EA group. Conclusion: Electroacupuncture alleviates myocardial fibrosis in rats with heart failure, and this effect is likely due to attributed to the modulation of the TGF-β1/Smads signaling pathway, which helps reduce collagen deposition in the extracellular matrix.

Inhibition of SLC26A4 regulated by electroacupuncture suppresses the progression of myocardial ischemia-reperfusion injury

Introduction:Myocardial ischemia-reperfusion(IR)injury has received widespread attention due to its damaging effects.Electroacupuncture(EA)pretreatment has preventive effects on myocardial IR injury.SLC26A4 is a Na+independent anion reverse transporter and has not been reported in myocardial IR injury.Objectives:Tofind potential genes that may be regulated by EA and explore the role of this gene in myocardial IR injury.Methods:RNA sequencing and bioinformatics analysis were performed to obtain the differentially expressed genes in the myocardial tissue of IR rats with EA pretreatment.Myocardial infarction size was detected by TTC staining.Serum CK,creatinine kinase-myocardial band,Cardiac troponin I,and lactate dehydrogenase levels were determined by ELISA.The effect of SLC26A4 on cardiomyocyte apoptosis was explored by TUNEL staining and western blotting.The effects of SLC26A4 on inflammation were determined by HE staining,ELISA,and real-time PCR.The effect of SLC26A4 on the NF-κB pathway was determined by western blotting.Results:SLC26A4 was up-regulated in IR rats but downregulated in IR rats with EA pretreatment.Compared with IR rats,those with SLC26A4 knockdown exhibited improved cardiac function according to decreased myocardial infarction size,reduced serum LDH/CK/CK-MB/cTnI levels,and elevated left ventricular ejection fraction and fractional shortening.SLC26A4 silencing inhibited myocardial inflammation,cell apoptosis,phosphorylation,and nuclear translocation of NF-κB p65.Conclusion:SLC26A4 exhibited promoting effects on myocardial IR injury,while the SLC26A4 knockdown had an inhibitory effect on the NF-κB pathway.These results further unveil the role of SLC26A4 in IR injury.

Study of the potential mechanism of acupuncture treatment for functional dyspepsia based on association rule mining and bioinformatics/network pharmacology approach

Objective:To explore the main acupoint prescription and mechanism of acupuncture in treating Functional dyspepsia(FD)by analyzing the clinical randomized controlled trial literature on acupoints and targets of acupuncture in the treatment of FD combined with association rule mining and bioinformatics/network pharmacology methods.Methods:Search relevant clinical randomized controlled trial literature on acupoints and therapeutic targets of acupuncture in the treatment of FD from eight databases from their inception to June 18th,2022.We obtained acupuncture selection points,meridian,and therapeutic targets and established acupoints database for acupuncture treatment of FD.FD-related targets were collected from GeneCards,DisGeNET,OMIM,and DrugBank databases.We obtained the potential targets of acupuncture on FD by taking the intersection of acupuncture for FD therapeutic targets and FD-related targets.We performed the protein-protein interaction network,Gene Ontology(GO)analysis,and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis.Results:In this study,26 Randomized Controlled Trials related to acupuncture treatment of FD were retrieved.We obtained 29 acupoints,17 acupuncture for FD therapeutic targets,and 10 intersection targets.Tumor necrosis factor(TNF),Interleukin(IL)-1,and Neuropeptide Y(NPY)are key targets of acupuncture in the treatment of FD.Conclusion:ST36-CV12-PC6-LR3 is the main acupoint prescription for FD.Acupuncture may affect Neuroactive ligand-receptor interaction,Gastric acid secretion,and IL-17,TNF signaling pathways by regulating related key targets,and play a synergistic role in the treatment of FD by inhibiting gastric acid secretion,alleviating inflammatory response,regulating the brain-gut axis,improving mood and other aspects.

A Multicentral Randomized Control Study on Clinical Acupuncture Treatment of Bell's Palsy

To confirm the clinical therapeutic effects of acupuncture and moxibustion on Bell’s palsy.Methods:480 cases from 4 hospitals were enrolled for this study,among whom 439 cases completed thewhole course of the study.The patients were randomly divided into the following 3 groups,a controlgroup(treated with prednisone,vitamin Bl,vitamin B12and dibazol),an acu-moxibustion group（treatedwith filiform needle plus moxibustion）,and a basic treatment plus acu-moxibustion group（treated withoral medicine like those in the control group plus acupuncture,and with moxibustion like in theacu-moxibustion group）.The whole treatment course lasted 4 weeks.The therapeutic effects wereevaluated according to the symptoms and signs,House-Brackmann grading scale and facial disabilityindexes（FDI）.Results:All the 4 centers（hospitals）completed this study well,with no statisticallysignificant difference found among the 4 centers in therapeutic effects.The patients with differentconditions were well distributed in the 3 groups,thus the basic general,data were comparable（P>0.05）.The therapeutic effects of the two treatment groups were better than the control group（respectivelyP<0.05 and P<0.01）,and it was the best in the acu-moxibustion group（P<0.01）.Conclusion:Acupuncture and moxibustion may exert definite therapeutic effects on Bell’s palsy,better than that ofthe basic treatment group or the basic treatment plus acu-moxibustion group.

CLINICAL STUDY ON ACUPUNCTURE IN THE TREATMENT OF POST-STROKE DEPRESSION WITH THE METHOD OF ACTIVATING THE DU MERIDIAN AND CLEARING THE MIND

Objective: To observe the therapeutic effect of acupuncture in the treatment of post-stroke depression (PSD) with the method of activating the Du Meridian and clearing the mind. Methods: Two hundred and thirty one cases of PSD were randomly divided into two groups, acupuncture and control groups. In acupuncture group, Baihui (百会 GV 20), Dazhui (大椎 GV 14), Neiguan (内关 PC 6), Taichong (太冲 LR 3) and Shenmen (神门 HT 7) were selected as the main points to invigorate flow of qi in the Du Meridian and clear the mind. Patients in the control group received Fluoxetine orally, 20 mg once per-day. In addition, the same routine treatments were given to both groups, strengthened with rehabilitation exercises as early as possible; 8 weeks constituted one therapeutic course. HAMD and severity of neuro-functional defect of each case were evaluated before and 8-week after the treatment. Results: The total effective rate of acupuncture group was 92.37%, the control group, 72.88%. According to χ2 test, the difference between the two groups was remarkably significant (P<0.05). Conclusion: Acupuncture therapy of activating the Du Meridian and clearing the mind in the treatment of PSD was safe and effective.

Effects of acupuncture on Alzheimer’s disease: the mechanistic study

Introduction Alzheimer’s disease(AD)is a degenerative disease of the central nervous system characterized by memory loss and cognitive impairment,often accompanied by emotional apathy,agitation,depression,delusions aggression,and other psychological symptoms[1–3].Psychological symptoms such as apathy,agitation,and depression tend to increase with the duration and severity of AD.

Massage and vocal training in a case of spasmodic dysphonia

A 35-year-old female patient with spasmodic dysphonia underwent massage and vocal training. Chinese manipulation was administered to Tiantu （CV 22） and Lianquan （CV 23）, the convergent acupoints of the conception meridian and Yin link vessel, Yamen （GV 15） and Fengfu （GV 16）, the convergent acupoints of Du meridian and Yang link vessel, as well as large intestine channel of hand Yangming Hegu （LI 4）, by gently pressing and rubbing for stimulation. In addition, throat muscle massage therapy and vocal training were performed. Results were evaluated using acoustic measurements in combination with a single statistical method. A comprehensive treatment combining vocal training and face-neck massage was shown to relieve dystonia of facial-cervical muscles in patients, as well as improve voice quality while providing a safe treatment method.

Effects of acupuncture combined with Kaijingtongmai Decoction on ATP sensitive potassium channel related proteins Kir6.1 and Kir6.2 in myocardial infarction rats

Objective:To study the effect of combination of acupuncture and medicine on the expression of ATP sensitive potassium channel related proteins Kir6.1 and Kir6.2 in rats with myocardial infarction,and to study the possible mechanism of combination of acupuncture and medicine on the improvement of myocardial infarction,so as to provide experimental data basis for the development of new treatment methods for myocardial infarction.Methods:65 healthy male SD rats were randomly selected as the control group.The other rats were fed with high-fat food for three weeks.The rats in the control group were injected with normal saline subcutaneously,and the other rats were injected with isoproterenol hydrochloride in the same way.Through ECG comparison,40 successful Mi rats were randomly divided into model group,acupuncture group,western medicine group and acupuncture drug combination group,with 10 rats in each group.After the corresponding treatment,the ECG changes of rats in each group were observed,the pathological changes of rat cardiomyocytes were observed by HE staining,and the expression of ATP sensitive potassium channel(Kir6.1,Kir6.2)protein was detected by Western blot.Results:compared with the control group,40 experimental specimens in the experimental group showed significant changes in cardiomyocyte protein The expression of Kir6.1 and Kir6.2 increased,and the difference was statistically significant.After treatment,compared with the model group,the protein expression of Kir6.1 and Kir6.2 in cardiomyocytes of Western medicine group,acupuncture group and acupuncture drug combination group showed a downward trend,among which the decline degree of acupuncture drug combination group was the most obvious,and the difference was statistically significant.The decline degree of acupuncture group and Western medicine group was not significant,and there was no significant difference Conclusion:acupuncture combined with medicine has a significant effect on improving myocardial infarction in rats,which may be related to the expression of ATP sensitive potassium channel related proteins Kir6.1 and Kir6.2 in rat cardiomyocytes.

Acupuncture Treating Heart Diseases Based on Sixty JiaZi and Eight Palaces <br/>—Mathematical Reasoning of Treatment Principle Based on Yin Yang Wu Xing Theory in Traditional Chinese Medicine (V)

Theory of Sixty JiaZi (60 甲子) is useful in understanding a human body disease. By using mathematical reasoning based on Yin Yang Wu Xing Theory in Traditional Chinese Medicine (TCM), this paper demonstrates the treatment principle: “seize the momentum of development, hasten lucky avoids disaster” (顺势而为, 趋吉避凶). Sixty JiaZi is hidden behind eight palaces. The first or second transfer law of human body energies of Sixty JiaZi changes according to the different blood pH values of a human body whether in the normal range or not, respectively, assuming that the range of blood pH values of a human body is divided into four parts from small to large. Both second and third are for a healthy human body. Both the root-cause and symptoms come from the first transfer law of human body energies. And both first and fourth are for an unhealthy human body. Both the root-cause and symptoms come from the second transfer law of human body energies. The treating disease of a human body should protect and maintain the balance of two incompatibility relations: the loving relationship and the killing relationship. As an application, acupuncture is used to treat atrial premature beats as congenital heart disease.

Identifying the effective combination of acupuncture and traditional Chinese medicinal herbs for postmenopausal osteoporosis therapy through studies of their molecular regulation of bone homeostasis

Worldwide,as the population age,osteoporosis is becoming increasingly common,and osteoporotic fractures have a significant economic burden.Postmenopausal women are the most susceptible to developing osteoporosis and the most critical time to prevent it is during the perimenopausal and early menopausal years.In this regard,we hypothesize rational combination of acupuncture and Traditional Chinese Medicine(TCM)in the form of herbal extract could prevent osteoporosis in women.Estrogen deficiency during menopause causes low-level inflammation that stimulates the formation of osteoclasts,the boneresorbing cells,and simultaneously inhibits the viability and function of osteoblasts,the bone-forming cells.The most potent inflammatory cytokine in skeletal homeostasis is the receptor activator of nuclear factor kappa B ligand(RANKL)that stimulates osteoclast function.Conversely,the canonical Wnt pathway is essential for osteoblastogenesis and bone formation,and estrogen deficiency leads to diminished functioning of this pathway.TCM and acupuncture could target the RANKL and the Wnt pathway in favorable ways to prevent the accelerated bone loss experienced during the early menopausal stage and promote the gain in bone mass in postmenopausal women.In this review,we propose a rational combination of specific TCM and acupuncture targeting those signaling molecules/pathways by the drugs that are in clinical use for the treatment of postmenopausal osteoporosis.Our rational approach revealed that Danshen(Radix Salviae Miltiorrhizae)could exert a synergistic effect with acupuncture.We then propose a translational path for developing the putative combination in women with postmenopausal osteoporosis to curtail the risk of osteoporotic fractures.

Effect of electroacupuncture at Sibai on the gastric myoelectric acitivities of denervated rats

AIM： To explore the mechanism of the exciting effects of electro-acupuncture （EA） at Sibai on the gastric myoelectric activities. METHODS： A total of 32 rats were randomly divided into four groups. Through intraperitoneal injection with atropine （the anti-cholinergic agent by blockade of muscarinic receptors）, hexarnethonium （automatic nerve ganglion-blocking agent） and reserpine （anti-adrenergic agent by depleting the adrenergic nerve terminal of its norepinephrine store）, effects of EA at Sibai on the gastric myoelectric activities of the denervated rats were observed. RESULTS： After intraperitoneal injection of atropine and hexamethonium, the average amplitude and ratio of period to time in the phase of high activity of gastric myoelectric slow wave, and the average numbers of the peaks of gastric myoelectric fast wave were significantly decreased （P 〈 0.01, P 〈 0.05, P 〈 0.01）, while after intraperitoneal injection of reserpine, the aforementioned three parameters were increased （P 〈 0.01, P 〈 0.05, P 〈 0.01）. EA at Sibai point partially relieved the inhibitory effect of atropine and hexamethonium on the gastric myoelectric activities in the rats （P 〈 0.05 or P 〉 0.05）. CONCLUSION： Cholinergic and adrenergic nervous systems and autonomic nerve ganglion participate in the peripheral passage of the controlling effects of EA at Foot Yangming Channel on gastrointestinal tract.

Combined treatment using acupuncture and music therapy on children with cerebral palsy Gross motor function measure comparison in 60 cases

BACKGROUND： The use of acupuncture has received recognition to effectively treat cerebral palsy. Moreover, music therapy can be used to modify treatment of cerebral palsy. OBJECTIVE： To study the effects of combined treatment using acupuncture and music therapy on gross motor function measure （GMFM） of children with cerebral palsy, compared with acupuncture treatment alone. DESIGN, TIME AND SETTING： Randomized, controlled, clinical study. The experiment was conducted in Shenzhen Hospital of Traditional Chinese Medicine between January 2007 and September 2007. PARTICIPANTS： All children with cerebral palsy in the trial were from the outpatient department of Shenzhen Hospital of Traditional Chinese Medicine. The children were randomly divided into two groups： 30 children in Group B received acupuncture and music therapy, while 30 children in Group A received only acupuncture therapy. METHODS： Subjects in Groups A and B received acupuncture based on syndrome differentiation. The main acupoints were necessary for all participants. At first, flash needling was applied to the acupoints. For the remaining acupoints, the technique of transverse needling was applied to the head acupoints, and perpendicular needling was used for the other points. The inserted needles were twirled and then maintained for 30 minutes. The needle was twirled for one second every other 10 minutes, without reinforcing-reducing techniques. The therapy was performed every other day. The trial consisted of three periods each, and lasted for 84 days. Subjects in Group B received music therapy. They listened to music that they preferred while acupuncture was being performed. Following acupuncture, they were allowed to perform musical activities, such as percussion, singing, and dancing. The music therapy was scheduled for one hour, including listening to music for 30 minutes and music activities for 30 minutes. MAIN OUTCOME MEASURES： The comprehensive functional evaluation scale of cerebral palsy and the GMFM were applied to test symptoms before and after respective treatment. RESULTS： All 60 children were included in final analysis. The general function of all children with cerebral palsy improved, but the general efficiency in Group B was obviously higher than in Group A （93% and 73%, Х^2 = 4.32, P 〈 0.05）. GMFM evaluation showed that the function of crawling and kneeling, standing, and walking improved more significantly in Group B than in Group A （P 〈 0.05）, while the function of lying down, rolling, and sitting remained similar between the two groups （P 〉 0.05）. CONCLUSION： The combination of acupuncture and music therapy on the treatment of cerebral palsy is more effective than acupuncture alone. The various activities in music therapy may affect treatment of cerebral palsy.

Thirty-six Cases of Urinary Retention Treated by Acupuncture

Objective:To observe the clinical therapeutic effects of acupuncture on urinary retention. Methods: 36 cases of urinary retention were treated by acupuncture at the following points: 1) Qugu (CV 2),Zhongji (CV 3) Guanyuan (CV 4),Sanyinjiao (SP 6) and Yinlingquan (SP 9); 2) Shenshu (BL 23),Pangguangshu (BL 28),Ciliao (BL 32) and Weiyang (BL 39). Results: 30 cases were cured,4 cases improved and 2 cases failed,with a curative rate of 83.33% and a total effective rate of 94.44%. Conclusions: Acupuncture is affirmatively effective in treating urinary retention.

Acupuncture Treating Heart Disease Based on Eight Palaces or Eight Veins <br/>—Mathematical Reasoning of Treatment Principle Based on Yin Yang Wu Xing Theory in Traditional Chinese Medicine (IV)

Theory of eight palaces (八宫) or eight veins (八脉) is useful in understanding the basic sick subsystems that may be to fall ills as the root-cause. By using mathematical reasoning based on Yin Yang Wu Xing Theory in Traditional Chinese Medicine (TCM), this paper demonstrates the treatment principle: “Even if all changed, it is hard to change one’s nature” (江山易改, 本性难移). The eight palaces or eight veins can be used to diagnose basic sick subsystems that may be to fall ills as the root-cause. The first or second transfer law of human body energies of the eight palaces or eight veins according to the different blood pH values of a human body whether in the normal range or not, respectively, assuming that the range of blood pH value is divided into four parts from small to large. Both second and third are for a healthy human body. Both the root-cause and symptoms come from the first transfer law of human body energies. And both first and fourth are for an unhealthy human body. Both the root-cause and symptoms come from the second transfer law of human body energies. A disease treatment should protect and maintain the balance of two incompatibility relations: the loving relationship and the killing relationship. As an application, acupuncture is used to treat atrial premature beats as a congenital heart disease.

Electropuncture influences on learning, memory, and neuropeptide expression in a rat model of vascular dementia

BACKGROUND： Studies in recent years have indicated that several neuropeptide-like substances, such as arginine vasopressin （AVP）, somatostatin （SS）, and β-endorphine （β-EP）, are involved in the process of cerebral ischemic damage to cranial nerves. OBJECTIVE： To observe the effects of electropuncture on back-shu points, as well as the influence on learning and memory, AVP, SS, and β-EP levels in plasma and brain were measured in a rat model of vascular dementia （VD）. DESIGN： Randomized controlled trial. SETTING： College of Acupuncture and Massage of Guangzhou University of Chinese Medicine. MATERIALS： This experiment was performed at the Animal Experiment Center of Guangzhou University of TCM from December 2005 to December 2006. A total of 48 healthy adult male Sprague Dawley rats of SPF-grade, 180-220 g, were provided by The Animal Experiment Center of Guangzhou University of Traditional Chinese Medicine. The following instruments were used： SDQ-30 Dipolar Radio-frequency Electrocoagulator （Shanghai Operation Instrument Factory）, Morris Water Maze （The Animal Experiment Center of Guangzhou University of Traditional Chinese Medicine）, Type G6805-1 Treating Equipment （Huasheng Equipment Factory, Qingdao, China）. METHODS： ① Eight rats were randomly selected for the control group; the remaining 40 rats underwent 4-vascular occlusion to establish a cerebral ischemia model. Due to the death of 13 rats and 2 hemiplegies during model establishment, there was a total of 25 model rats available for testing. The model rats were divided randomly into 3 groups according to their body weight： electropuncture group （n = 9）, medication group （n = 8）, and VD group （n = 8）. ② Electropuncture group： 25 mm needles （28 gauge） were used to electropuncture （150 Hz, continuous waves, 1.0-2.0 mA, duration of 20 minutes） the following acupoints： Baihui （GV20）, Geshu （BLIT）, Pishu （BL20）, and Shenshu （BL23）. The acupoints were located according to Experimental acupuncturology and were stimulated electrically by G6805-1 treating equipment. Medication group： Nimotop （certificate number： 110156; 0.6 mg/mL suspension）, 20 mL/kg, was perfused once daily intragastrically for 15 days. Model group： the rats were fed once daily with 150 g/L isotonic NaCl for 15 days （20 mL/kg）. MAIN OUTCOME MEASURES： Morris water maze testing was employed to study learning and memory behavior. Levels of AVP, SS, and β -EP were measured in plasma and brain radioimmunoassay （RIA）. RESULTS： A total of 33 Sprague Dawley rats were included in the final analysis. In the VD group, AVP and SS levels in plasma and brain were lower than the other groups （P 〈 0.01）. However, β-EP plasma levels decreased significantly （P 〈 0.01）, while β-EP brain levels increased significantly （P 〈 0.01）. The AVP and SS content in plasma and brain, as well as β -EP in plasma, increased （P 〈 0.01）. The levels of β-EP in the brain decreased （P 〈 0.01）. Morris Water maze results demonstrated that the electropuncture, medication, and control groups had shorter escape times than the VD group （P 〈 0.01）. Animals in the electropuncture, medication, and control groups spent more time in the platform quadrant than in the other three quadrants （P 〈 0.01 ）. CONCLUSION： Electropuncture can regulate the amount of AVP, SS, and β-EP in the plasma and brain, and correlates with improved learning and memory in a rat model of VD.

Dr. Lai Xinsheng’s Experience in Acupuncture Treatment of Refractory Diseases

Having been engaged in teaching, clinical practice and research for many years, Dr. Lai Xinsheng is good at acupuncture and the combined acupuncture and medication therapy to treat refractory diseases with satisfactory therapeutic effect. Sample cases treated by Dr. Lai are selected as follows.

Effect of posterior root amputation on rabbit knee joint cartilage and proprioception

Objective:To Observe the characteristics of changes in the cartilage of the rabbit's knee joint after spinal nerve transection,as well as the influence of proprioceptors and proprioceptive pathways,and explore the relationship between proprioception disorder and knee osteoarthritis.Methods:According to the principle of random allocation,20 New Zealand white rabbits were divided into groups,9 in the control group and 11 in the model group.In the model group,the posterior roots of the L5-L6 spinal nerve were cut off using the posterior root cutting method,while the control group only cut the skin and paraspinal muscles.8 weeks after the operation,the modified Tarlov scoring standard was used to evaluate the behavioral differences between the two groups of rabbits,and electrophysiology-SEPs(Somatosensory Evoked Potentials)and EMG(Surface Electromyography)were used to evaluate proprioception and muscle contraction.After the test was completed,it was unified They were sacrificed and performed HE staining.Mankin's score was used to evaluate the pathological conditions of the knee articular cartilage in the two groups.The anterior cruciate ligament(ACL)of the right knee was taken and the Bielschowsky nerve staining method was used to observe the number of proprioceptors in the two groups.Results:8 weeks after the operation,the hind limbs of the model group showed a light stroke posture,and the motor function basically returned to normal.Compared with the control group,there was no significant difference in behavioral changes(P>0.05);the model group rabbits SEPs and EMG were compared with the control group Compared with the prolonged incubation period,the amplitude decreased,and the difference was statistically significant(P<0.05).The rabbit articular cartilage surface of the model group was rough or even disappeared,and the cell arrangement was disordered.The Mankin's score was significantly higher than that of the control group(P<0.01),and cartilage damage was more severe.Compared with the control group,the number of proprioceptors in the model group was significantly reduced,the morphology changed,and the ligament tissue was loose and constricted.The difference was statistically significant(P<0.01);the rabbit chondrocytes in the model group were over-apopted and the rate of apoptosis It was significantly higher than the control group(P<0.01).Conclusion:The transection of the spinal nerve can lead to loss of proprioception,excessive apoptosis of chondrocytes,degeneration of articular cartilage,and promotion of the occurrence and development of knee osteoarthritis.

Clinical observation of sinew-regulating and bone-setting manipulation combined with Xiaoyu Jiegu Powder in treating knee osteoarthritis

Objective:To evaluate the clinical efficacy of sinew-regulating and bone-setting manipulation(SRBSM)combined with Xiaoyu Jiegu Powder(XYJGP)in treating knee osteoarthritis(KOA).Methods:Sixty patients were randomly divided into treatment group(30 cases)and control group(30 cases).The treatment group was treated with SRBSM combined with XYJGP and the control group was given intermediate frequency electrotherapy and XYJGP.Visual Analogue Scale(VAS).The Western Ontario and McMaster Universities Osteoarthritis Index(WOMAC),Flexion range of motion of the knee joint,and surface electromyography(sEMG)were used to evaluate and compare the patients'knee function before and after treatment.The efficacy was observed with diagnosis and efficacy standards of TCM.Results:After treatment,compared with the same group before treatment,muscle fatigue and muscle tension decreased,pain was reduced,range of motion of the knee joint was increased,and motor function was improved(P<0.05).The improvement degree of the treatment group was better than that of the control group(P<0.05).The total effective rate of the treatment group was 96.6%(29/30),higher than that of the control group(80.0%),P<0.05.Conclusion:SRBSM combined with XYJGP can effectively reduce the pain of KOA patients,improve the range of motion of the joints,and improve the quality of life of the patients,and its curative effect is better than that of intermediate frequency electrotherapy combined with XYJGP.

Effect of sinew-regulating and bone-setting manipulating combined with exercise training in the treatment of joint injury of distal radio-ulnar

Objective: To observe the effect of the joint injury of the distal radio-ulnar joint. Methods: 60 patients with Distal Radioulnar Joint (DRUJ) injury were divided into observation group and control group according to random number method. 30 cases were included in each of the two groups.Before and after treatment in patients with Visual Analogue Scale (Visual Analogue Scale, VAS) score, forearm pronation and supination electromyographic activity, methods of electric integral value (integral electromyogram, iEMG) and Wrist in patients with self assessment Scale (Patient - Rated Wrist Evaluation, PRWE) score evaluation, comparison, and the clinical observation on diagnosis of disease and curative effect of traditional Chinese medicine standard (assessment process by blind method).Results: compared with the two groups before and after treatment, VAS score decreased, forearm pronation and postpronation activity increased, iEMG value increased, and PRWE scale score decreased (all P < 0.05), and the curative effect of the treatment group was better than that of the control group (P < 0.05). The total effective rate of the treatment group [93.3% (28/30)] was higher than that of the control group [50%(15/30), P < 0.05].Conclusion: the combined exercise training of muscle and bone setting technique can effectively alleviate the pain of patients with radial ulnar joint injury, improve the rotation of the forearm, increase the recruitment of the anterior rotatory muscle, and improve the wrist function of patients, and the effect is better than if combined with forearm support fixation.

Effect of tendon and bone setting manipulation combined with Kinesio Taping on de Quervain disease

Objective:To observe the effect of the treatment of tendon-regulation and bone-setting manipulation and Kinesio Taping on patients with de Quervain Disease.Methods:60 cases with de Quervain Disease were divided into 30 treatment group and 30 control group according to random number table.The observation group was treated withtendon-regulation and bone-setting manipulation and Kinesio Taping,and the control group was given Votalin emulsion combined with Kinesio Taping.The patients were evaluated and compared according to the VAS before and after treatment,the degree of tenderness,the methods of electric integral value(integral electromyogram,iEMG),the degree of wrist ulnar deviation,and the cooney wrist score,and the curative effect was observed using the diagnostic curative effect criteria of TCM disease(the evaluation process was blinded).Results:Compared with the same group before treatment,after treatment,the pain and tenderness of the two groups were reduced,iEMG value increased,the ulnar deviation of the wrist increased,and the cooney wrist score increased(all P<0.05);the improvement of the treatment group was significantly better.In the control group,the difference was statistically significant(P<0.05)and the total effective rate of the treatment group was 93%higher than that of the control group 80%(P<0.05).Conclusion:The combination of the tendon-regulation and bone-setting manipulation combined with the Kinesio Taping can effectively reduce the pain and tenderness of the patients withde Quervain Disease,increase the ulnar mobility of the wrist joint,and improve the joint function of the wrist joint.