Evaluating the biosignature potential of ammonium in Proterozoic red beds and implications for the search for life on Mars

Over the past two decades,it has become increasingly apparent that early Mars may once have been warmer,wetter and more habitable for microbial life than it is today,which has spurred discussions about potential biosignatures that may be preserved in Martian sediments.An impediment to this line of research is the pervasive oxidation of Mars’surface due to photochemical oxidants that have likely destroyed remnants of organic matter.Here,we investigate whether nitrogen(N)transferred from biomass to phyllosilicate minerals during diagenesis can be preserved in oxidized mudrocks.We investigate two sequences of terrestrial Proterozoic red beds,namely the Sibley Group(1.4 Ga)in Canada and the Stoer Group(1.2 Ga)in Scotland,and we find enrichments in authigenic N in the range of several tens of ppm in both units.The highest concentrations(ca.100 ppm on average)are found in the most desiccated red beds of the Stoer Group,concurrent with enrichments in potassium(K).We discuss similarities and differences between the two sets of rocks with regards to salinity,pH,biological productivity and K-metasomatism,and we conclude that the ideal mechanism for the preservation of biogenic N in red beds may be in-situ release of ammonium from microbial mats into the clay substrate,possibly facilitated by early diagenetic,biologically induced illitization.Illite and smectite have been observed on Mars,and experiments suggest that Martian waters contained moderate amounts of dissolved K.Hence,it is conceivable that a similar K and N enrichment process could have occurred as to what we document for the Proterozoic,preserving evidence of life that may have survived to the modern day.

Determining the spatial distribution of soil properties using the environmental covariates and multivariate statistical analysis: a case study in semi-arid regions of Iran

Natural soil-forming factors such as landforms, parent materials or biota lead to high variability in soil properties. However, there is not enough research quantifying which environmental factor(s) can be the most relevant to predicting soil properties at the catchment scale in semi-arid areas. Thus, this research aims to investigate the ability of multivariate statistical analyses to distinguish which soil properties follow a clear spatial pattern conditioned by specific environmental characteristics in a semi-arid region of Iran. To achieve this goal, we digitized parent materials and landforms by recent orthophotography. Also, we extracted ten topographical attributes and five remote sensing variables from a digital elevation model(DEM) and the Landsat Enhanced Thematic Mapper(ETM), respectively. These factors were contrasted for 334 soil samples(depth of 0–30 cm). Cluster analysis and soil maps reveal that Cluster 1 comprises of limestones, massive limestones and mixed deposits of conglomerates with low soil organic carbon(SOC) and clay contents, and Cluster 2 is composed of soils that originated from quaternary and early quaternary parent materials such as terraces, alluvial fans, lake deposits, and marls or conglomerates that register the highest SOC content and the lowest sand and silt contents. Further, it is confirmed that soils with the highest SOC and clay contents are located in wetlands, lagoons, alluvial fans and piedmonts, while soils with the lowest SOC and clay contents are located in dissected alluvial fans, eroded hills, rock outcrops and steep hills. The results of principal component analysis using the remote sensing data and topographical attributes identify five main components, which explain 73.3% of the total variability of soil properties. Environmental factors such as hillslope morphology and all of the remote sensing variables can largely explain SOC variability, but no significant correlation is found for soil texture and calcium carbonate equivalent contents. Therefore, we conclude that SOC can be considered as the best-predicted soil property in semi-arid regions.

Comparison and Evaluation of Two Analytical Methods for Cation Exchange Capacity and Exchangeable Sodium Percentage of Five Soil Types in Central Sudan

Twenty-six soil samples were collected from five soil profiles at different climatological and ecological regions in central Sudan. Soil profile was dug in each studied area and morphological profile description was carried out for different horizons. All samples were analyzed using two different methods to determine Cation Exchange Capacity (CEC) and exchangeable sodium percentage (ESP). Statistical analysis (T-test) was used in order to investigate the differences between soil samples for the studied locations. Significant differences appeared when compared the two methods for CEC determination at Gedaref area, Wad Medani and Nile flood plain and that appeared in evaluation of ESP at Nile flood plain and Shambat area. The results also revealed that, the developed method used in this study was more practical, simple and reliable for determination of CEC and ESP as the currently used in most soil laboratories. In addition, it will be safer than the other methods in some problematic soils. The adoption of this developed method is advisable because it is less time consuming as it omits the washing step. In contrast, the old method cannot be a good substitute in laboratories which have no possibility to determine sodium by using flame photometer. We conclude that when the developed method is used to determine CEC and ESP time will be saved, that fewer amounts of chemicals will be used and that accurate results will be achieved.

Assessment of Calcimetric and Titrimetric Methods for Calcium Carbonate Estimation of Five Soil Types in Central Sudan

Despite the CaCO3 estimation using titration method was not reliable, but up to the present time, some soil laboratories in Sudan still used this method. The objective of this study was to compare and assess the results of calcimetric and titrimetric methods of quantitative estimation for soil calcium carbonate of different soils in Sudan. 26 soil samples from five soil profiles were collected from different climatological and ecological regions in central Sudan. CaCO3 equivalent was estimated using calcimeter and titration methods in order to find accurate, rapid and suitable method for soils of Sudan. The results revealed that there are no significant differences between calcimeter and titration methods for calcium carbonate estimation in all studied samples except in samples from Gedaref area. We concluded that when the Calcimeter method used for CaCO3 estimation, the differences between one person and another in detecting titration end point would be avoided, rapid and accurate results would be obtained compared to titration method. Additionally, time would be saved;fewer amounts of chemicals would be used. From this study, we highly recommend using calcimeter method for CaCO3 estimation for soils of Sudan.

Genesis and Classification of Some Soils of the River Nile Terraces: A Case Study of Khartoum North, Sudan

Soils developed in the alluvium terraces of the River Nile at Khartoum North, Sudan was analyzed in an attempt to classify it as well as to refer them to their origin. Three river terraces comprising nine profiles were selected to cover the physiographic positions. Lack of B horizon and carbonate accumulation were main pedogenic processes in subsurface horizons, whereas orhric epipedon was developed on top soil surface. The microscopic inspection of heavy sand mineralogy indicated that the origin of the sand was the Ethiopian plateau. The most abundant clay mineral was smectite, followed by illite, kaolinite and chlorite. The presences of micas (illite) and chlorite in all studied soil samples might emphasize that these soils were young from the pedological viewpoint and less weathered. The soils of the River Nile terraces at Khartoum North were classified into: Typic Torrifluvents (1st terrace), Entic Haplocambids (2nd terrace) and Typic Haplocambids (3rd terrace). Mineralogy analysis indicated that the Entisols and Aridisols of the River Nile terraces in the study area had the same origin that of the igneous and metamorphic rocks from Ethiopian plateau.

Mutual Adsorption of Lead and Phosphorus onto Selected Soil Clay Minerals

Mutual adsorption of lead (Pb) and phosphorus (P) at pH 5 onto three soil clays materials (kaolinite, montmorillonite, and allophane) was studied to know interaction of the anion and the cation at surface of the clays. Adsorption of Pb was determined on montmorillonite, kaolinite and allophane with the following pretreatments;1) untreated clay (control), 2) phosphate treated clay (P-clay) and 3) clay pre-treated with both P and Pb (P-Pb-clay). Adsorption of P was determined on montmorillonite, kaolinite and allophane with the following pretreatments;1) control 2) Pb treated clay (Pb-clay) and 3) P-Pb-clay. The adsorption of Pb on the untreated clays was in the order: montmorillonite > allophane > kaolinite. On allophane and kaolinite Pb adsorption was in the order P-clay > P-Pb-clay > control. For montmorillonite, the trend was: P-Pb-clay = control > P-clay. Phosphorus adsorption was in the order Pb-clay = P-Pb-clay > control for montmorillonite and kaolinite, Pb-clay > control > P-Pb-clay for allophane. The findings suggested that pre-treatment with phosphate increases Pb adsorption on kaolinite and allophane, and decrease on montmorillonite, while pretreatment with Pb increases phosphate sorption on all clays, and both Pb and P increased adsorption on montmorillonite and kaolinite and decrease on allophane.

Origin and Distribution of Heavy Minerals in the Surficial and Subsurficial Sediments of the Alluvial Nile River Terraces

Origin and distribution of the heavy minerals of surficial and subsurficial sediments has been investigated in the alluvial Nile River terraces, Khartoum North, Sudan. Heavy mineral assemblages in the very fine sand fraction (0.063 - 0.125 mm) of 10 sediment samples were identified using petrography microscope. Results of descriptive statistical parameters revealed that most sediments samples belonged within very poorly sorted to extremely poorly sorted, strongly negative skewed to strongly positive skewed and mesokurtic to very leptokurtic. The quartz was the dominant in the opaque minerals in all sediments. The non-opaque heavy minerals were dominant by zircon, tourmaline, rutile, garnet, sillimanite, and andalusite. Results revealed that the ultrastable minerals (zircon, tourmaline and rutile) were found in all sediments with range from (2% - 47.36%, 2.08% - 29% and 3% - 24.99%), respectively. Garnet, sillimanite and andalusite were also found with range from (5% - 67%, 1% - 9.09% and 1% - 50%), respectively. Heavy mineral assemblage indentifies sources that are not bounded to the local origin. The proportion and presence of heavy minerals from outside source rocks indicated relatively strong reworking of zircon sand from the outer-shelf to inner-shelf as well relatively long distance of transport. Fluvial and Aeolian sediments were the dominant environments in the investigated area. We conclude that most heavy minerals in the study area are originally derived from gneisses and schist metamorphic rocks and some igneous rocks of the Ethiopian plateau.

Winter Runoff of Nitrogen and Phosphorus from a Rotational Pen Design with Suckler Cows

Keeping beef cattle outdoors during winter reduces costs and improves animal welfare, but increases the risk of nitrogen (N) and phosphorus (P) runoff losses. This study evaluated a rotational pen design on grassland with two groups of suckler cows given access to an expanding staying area and a new feeding area each week (72 cattle ha-1), with one month’s stay per pen. The spatial distribution of excreta and effects on N and P surface runoff was evaluated during six months. The total excreta loads corresponded to 500 kg·N·ha-1 and 50 kg·P·ha-1. New feeding areas did not distribute excretions evenly, which resulted in the highest proportion of excretions (31%) occurring in the first week’s sub-area. The topsoil had significantly higher amounts of mineral-N, mainly as NH4-N (29 - 81 kg·ha-1), than an unaffected area (13 kg·ha-1). Mean total runoff losses were similar for both groups (1.4 kg·P·ha-1 and 9.0 kg·N·ha-1). Around 78% of N and 70% of P runoff losses occurred during the month with cattle present. During the first two weeks with heavy rain, N and P runoff losses were 50% higher from an area with suckler cows than a corresponding vegetated sub-area without cows. The study design did not provide a sufficient distribution of excretions and a high animal density in combination with trampling resulted in unacceptable N and P run-off losses. An environmentally friendly design would need to include frequent moving of all equipment and access to larger areas.

Knowledge, Attitude and Practices Used in the Control of Striga in Maize by Smallholder Farmers of Western Kenya

The production of maize is constrained by parasitic weeds, particularly Striga. A study was carried out to document farmers＇ knowledge, attitude and practices on Striga control among smallholder farmers across three districts： Kisumu West, Busia and Teso South of Western Kenya. A multistage sampling technique was used to select the locations and farmers to be interviewed. A semi structured, open and closed ended questionnaire was administered leading to field experiment. Besides village meetings （39.2%）, farmers got informed on farming methods under Striga weed farms and its control technologies through neighbours （2.5%）, workshops and trainings （5.0%）, field schools （3.7%）, media （7.5%） and extension agents （10.8%）. The attitudes of farmers towards Striga control varied but frequently cited： long term viability of the Striga seed （12.5%）, difficult to control sharing of farm tools （10.8%）, expensive technologies （13.3%）, lack of adequate information （18.3%）, labour intensive （15.0%）, large farms for use of push and pull technology （1.7%） and time consuming （12.5%）. Framers used various Striga control practices but traditional methods （25%） were among the most used （25%）. Concerted effort involving researchers, extension agents and private sector are, therefore, required for wide scale dissemination and adoption of the existing modem control technologies.

Chemical and Morphological Characteristics of Particulate Matter Suspended in the Air of the Dhaka University Area of Bangladesh

Total suspended particulate matter (SPM) in the urban atmosphere of Dhaka city was collected using a high volume sampling technique for a period of one month (August 2017-September 2017). Chemical characterization of particulate matter (PM) was investigated, and characterized concerning the size distribution, morphological features such as count, total area, average size, perimeter, circularity, aspect ratio (AR), roundness;equivalent spherical diameter (ESD), surface area and volume of PM. The results of elemental analysis showed that the presence of heavy metal in PM was ten to hundred times higher than the standard value prescribed by WHO and USEPA. Several morphological analysis indicated that particles varying in shape from nearly spherical to various irregular shape, had higher surface energy, higher content of Cl and S bearing particles and had a large surface area which can cause greater damage to lungs. The presence of various organic compounds containing functional groups like alcohols, ketones aldehydes, carboxylic acids as well as unsaturated and saturated carbon bonds was observed by FT-IR analysis. Scanning Electron Microscopy (SEM) was used to characterize the morphology of the PM. Agglomerates and shoots type particles were mostly seen in SPM.

Influence of the Storage Duration at Different Temperatures on the Concentrations of Extractable Inorganic Soil Nutrients

The influence of the short storage periods at different temperatures on the concentrations of extractable soil cations (Ca2+, Mg2+, Na+ and K+) and anions (Cl-, SO4- and PO4- ) has been investigated in nine soil samples from Nile river terraces at River Nile State, North of Sudan (17.82289 to 17.82389N and 33.99974 to 34.02127E). Each soil extract is divided into three treatments: i) control (immediately analyzed);ii) storage for 10 days and;iii) storage for 30 days. Each treatment is replicated three times: i) storage at 10°C;ii) storage at ambient laboratory temperature (25°C) and;iii) storage at 45°C in incubator. Statistical analysis of results reveals that significant difference are found at level (P - and PO4- (0.043, 0.002, 0.001, 0.021, 0.004 and 0.001) respectively at 25°C and 45°C and storage periods of 10 and 30 days. In contrast, significant difference is also found at level (P - concentrations are significantly decreased when the storage period exceeds 10 days and temperature more than 25°C. Depending upon our study results;we conclude that, all extractable inorganic nutrients are clearly affected by storage periods at various temperatures, exception of Na+. Most cations and anions are increased significantly with increased of storage period and temperatures. We therefore highly recommend that the extractable inorganic soil nutrients should be rapidly analyzed in order to obtain accurate results;otherwise, the time between extraction and analysis should be carefully recorded which may help considerably interpreting data from various studies.

Experimental Culture of the Giant Freshwater Prawn, Macrobrachium vollenhovenii (Herklots, 1857) around the FUTO Axis of Otamiri River, Imo State, Nigeria

Culture trial of 7,488 juveniles of the giant freshwater prawn, Macrobrachium vollenhovenii (Herklots, 1857) was carried out for 180 d in concrete tanks to determine their adaptability, survival, feed utilization and growth performance under culture condition. Twelve experimental concrete tanks each measuring 10 m × 5 m × 1.0 m were used for the experiment from March to August 2016. The prawns were stocked two weeks after treatment of tanks with agricultural lime at the rate of 2,279 kg/ha and poultry manure at the rate of 114 kg/ha. Four experimental diets tested were designated as T1: powdered broiler starter, T2: commercial brine shrimp, T3: combination of broiler starter and brine shrimp and T4: Mississippi Agricultural and Forestry Experimental Station (MAFES) diet formula. Diets were randomly assigned to the four treatment tanks and replicated three times in a 4 × 3 = 12 experimental units as a completely randomized design experiment (CRD). Each tank was stocked with 624 juvenile prawns with mean total length of 1.5 cm and body weight of 10.0 g. Prawns were fed at 5% body weight and twice at 0600 h and 1800 h. Sampling for growth was carried out bi-weekly while water quality was tested weekly. Results were statistically evaluated with one way analysis of variance (ANOVA). Water quality of the four tanks was not significantly different (p > 0.05) from one another throughout the culture period and conformed to recommended tolerable limits in prawn culture ponds. Growth performance was best in diet T4 (the MAFES formula), with a survival rate of 75%, mean weight gain (MWG) of 65 g, relative growth rate (RGR) of 650%, specific growth rate (SGR) of 1.12%, average daily growth (ADG) of 0.3 g/day and food conversion ratio (FCR) of 1.44. The above values differed quite significantly (p < 0.05) from growth responses of other diets. The computed gross ratio (GR) of 0.62 suggests that prawn culture is a highly lucrative business.

Dynamics of Soil Organic Carbon Fractions and Aggregates in Vegetable Cropping Systems

Fertilisers significantly affect crop production and crop biomass inputs to soil organic carbon(SOC). However, the long-term effects of fertilisers on C associated with aggregates are not yet fully understood. Based on soil aggregate and SOC fractionation analysis, this study investigated the long-term effects of organic manure and inorganic fertilisers on the accumulation and change in SOC and its fractions, including the C concentrations of free light fraction, intra-aggregate particulate organic matter(POM) and intra-aggregate mineral-associated organic matter(MOM). Long-term manure applications improved SOC and increased the concentrations of some C fractions. Manure also accelerated the decomposition of coarse POM(cPOM) into fine POM(fPOM) and facilitated the transformation of fPOM encrustation into intra-microaggregate POM within macroaggregates. However, the application of inorganic fertilisers was detrimental to the formation of fPOM and to the subsequent encrustation of fPOM with clay particles, thus inhibiting the formation of stable microaggregates within macroaggregates. No significant differences were observed among the inorganic fertiliser treatments in terms of C concentrations of MOM, intra-microaggregate MOM within macroaggregate(imMMOM) and intra-microaggregate MOM(imMOM). However, the long-term application of manure resulted in large increases in C concentrations of MOM(36.35%), imMMOM(456.31%) and imMOM(19.33%) compared with control treatment.

Soil Aggregate Stability and Iron and Aluminium Oxide Contents Under Different Fertiliser Treatments in a Long-Term Solar Greenhouse Experiment

Soil in greenhouses is likely to suffer a gradual decline in aggregate stability. Determination of the effects of different fertiliser practices on soil aggregate stability is important for taking advantage of solar greenhouses. Soil aggregate stability and iron （Fe） and aluminium （A1） oxide contents were investigated in a 26-year long-term fertilisation experiment in greenhouse in Shenyang, China, under eight fertiliser treatments： manure （M）, fertiliser N （FN）, fertiliser N with manure （MN）, fertiliser P （FP）, fertiliser P with manure （MP）, fertiliser NP （FNP）, fertiliser NP with manure （MNP）, and control without any fertiliser （CK）. A wet sieving method was used to determine aggregate size distribution and water-stable aggregates （WSA）, mean weight diameter and geometric mean diameter as the indices of soil aggregate stability. Different fertiliser treatments had a statistically significant influence on aggregate stability and Fe and A1 oxide contents. Long-term application of inorganic fertilisers had no obvious effects on the mass proportion of aggregates. By contrast, manure application significantly increased the mass proportion of macroaggregates at the expense of microaggregates. All treatments, with the exception of FNP, significantly increased the stability of macroaggregates but decreased that of microaggregates when compared with CK. Aggregation under MP and MN was better than that under M and MNP; however, no significant differences were found among inorganic fertiliser treatments （i.e., FN, FP, and FNP）. A positive relation was found between pyrophosphate-extractable Fe and WSA （r=0.269）, but no significant relations were observed between other Fe and Al oxides and aggregate stability.

Chemical Properties and Biochemical Activity of Colonized and Abandoned Litter-Feeding Termite(Macrotermes spp.) Mounds in Chromic Cambisol Area on the Borana Plateau, Ethiopia

Termite （Macrotermes spp.） mounds are complex biological habitats originated by the termite activity and possessing peculiar physical, chemical and biochemical properties. In this study we examined the concentration of nutrients and the biochemical activity of abandoned soil and mounds colonized by termites of the genera Macroterrnes located in the Borana District, Ethiopia. To elucidate the magnitude and persistence of the termite-induced effects, we also studied an abandoned mound, previously colonized by termites of the same genera formed on the same soil. Results confirmed that termite-colonized mounds are ＇hot spots＇ of nutrient concentration and microbial activity in tropical soils. This is due to the termite driven litter input and decomposition. The abandoned mounds showed higher microbial biomass and activity and displayed a nutrient redistribution and a greater microbial activity than the adjacent soils. These findings allowed us to hypothesize a model of nutrient cycling in colonized soils and a partition of the relative roles of termites and soil microorganisms in nutrient location and turnover in tropical soils. These results may be also useful for the optimal management of termite-colonized soils.

Life cycle assessment of urban uses of biochar and case study in Uppsala,Sweden

Biochar is a material derived from biomass pyrolysis that is used in urban applications.The environmental impacts of new biochar products have however not been assessed.Here,the life cycle assessments of 5 biochar products(tree planting,green roofs,landscaping soil,charcrete,and biofilm carrier)were performed for 7 biochar supply-chains in 2 energy contexts.The biochar products were benchmarked against reference products and oxidative use of biochar for steel production.Biochar demand was then estimated,using dynamic material flow analysis,for a new city district in Uppsala,Sweden.In a decarbonised energy system and with high biochar stability,all biochar products showed better climate performance than the reference products,and most applications outperformed biomass use for decarbonising steel production.The climate benefits of using biochar ranged from−1.4 to−0.11 tonne CO_(2)-eq tonne−1 biochar in a decarbonised energy system.In other environmental impact categories,biochar products had either higher or lower impacts than the reference products,depending on biochar supply chain and material substituted,with trade-offs between sectors and impact categories.However,several use-phase effects of biochar were not included in the assessment due to knowledge limitations.In Uppsala’s new district,estimated biochar demand was around 1700 m^(3)year^(−1)during the 25 years of construction.By 2100,23%of this biochar accumulated in landfill,raising questions about end-of-life management of biochar-containing products.Overall,in a post-fossil economy,biochar can be a carbon dioxide removal technology with benefits,but biochar applications must be designed to maximise co-benefits.

Wheat yield prediction by zero sink and equilibrium-type soil phosphorus tests

Diffusive gradients in thin films(DGT)measurements have been shown to outperform other phosphorus(P)tests in soils with strong P sorption,but this has not been confirmed for moderately weathered European soils.We compared the performance of DGT in predicting wheat grain yield in Swedish long-term fertility experiments with those of standard intensity(water-extractable P(P-H2O))and quantity(ammonium lactate-extractable P(P-AL))tests.A Mitscherlich-type model was used to fit wheat yield response to P application rates(0,15,30 or 35,and 45 kg P ha-1 year-1)in each individual trial replicate to estimate the maximum yield.For trials with clear plateau-type yield responses and the goodness of fit(R2)>0.75,relative yields(RYs)were calculated for each P treatment and plotted against the soil P test results(n=143).The goodness of the Mitscherlich-type fits decreased in the following order:DGT-measured P(P-DGT)(R2=0.35)>P-H2O(R2=0.18)>P-AL(R2=0.13).When excluding soils with P-AL:P-DGT≥0.1 L g-1,R2 was considerably improved to 0.55 for P-AL,0.46 for P-H2O,and 0.65 for P-DGT(n=61).At 95%of maximum yield,the upper limit of P deficiency for P-DGT was 44.8(the soils with P-AL:P-DGT<0.1 L g-1)and 61.9μg L-1(all soils),falling within the range reported for other European and Australian soils(6.0–142μg L-1).We show that in the investigated Swedish soils,DGT performed better than the quantity and intensity tests,which is attributed to its ability to capture P diffusion and resupply from the soil solid phase,similar to plant roots in the rhizosphere.

FungalTraits:a user-friendly traits database of fungi and fungus-like stramenopiles

The cryptic lifestyle of most fungi necessitates molecular identification of the guild in environmental studies.Over the past decades,rapid development and affordability of molecular tools have tremendously improved insights of the fungal diversity in all ecosystems and habitats.Yet,in spite of the progress of molecular methods,knowledge about functional properties of the fungal taxa is vague and interpretation of environmental studies in an ecologically meaningful manner remains challenging.In order to facilitate functional assignments and ecological interpretation of environmental studies we introduce a user friendly traits and character database FungalTraits operating at genus and species hypothesis levels.Combining the information from previous efforts such as FUNGuild and FunFun together with involvement of expert knowledge,we reannotated 10,210 and 151 fungal and Stramenopila genera,respectively.This resulted in a stand-alone spreadsheet dataset covering 17 lifestyle related traits of fungal and Stramenopila genera,designed for rapid functional assignments of environmental stud-ies.In order to assign the trait states to fungal species hypotheses,the scientific community of experts manually categorised and assigned available trait information to 697,413 fungal ITS sequences.On the basis of those sequences we were able to summarise trait and host information into 92,623 fungal species hypotheses at 1%dissimilarity threshold.

Correction to:FungalTraits:a user friendly traits database of fungi and fungus-like stramenopiles

Correction to:Fungal Diversity(2020)105:116 https://doi.org/10.1007/s13225-020-00466-2 There were errors in the name of author LászlóG.Nagy and in affiliation no.31 in the original publication.The original article has been corrected.

Prospecting Exopolysaccharides Produced by Selected Bacteria Associated with Marine Organisms for Biotechnological Applications

In this study, bacteria associated with marine organisms were screened for the production of exopolysaccharides（EPSs） on MY media containing sea salts（2.5%-10%）. Three selected isolates were identified as Alteromonas sp. PRIM-21, Nitratireductor sp. PRIM-24 and Enterobacter sp. PRIM-26 using 16 S r RNA gene sequencing. Optimization of the growth and EPS production kinetics in relation to incubation time were assessed. The purified EPS yield was 590, 650 and 540 mg·L-1 culture media respectively in Alteromonas sp. PRIM-21, Nitratireductor sp. PRIM-24 and Enterobacter sp. PRIM-26. Biochemical and FTIR analyses revealed the presence of biologically important functional groups in the EPS produced by all the three isolates. The EPS produced by Nitratireductor sp. PRIM-24 and Alteromonas sp. PRIM-21 showed 2.0% sulfate content. These bacterial EPS also showed antioxidant and emulsifying activities and the EPS produced by Enterobacter sp.PRIM-26 showed significantly higher antioxidant activities in terms of superoxide（IC50 0.33 mg·mL -1） and DPPH（IC50 0.44 mg·mL -1） radical scavenging. It also showed higher emulsifying activities against selected hydrophobic substrates with EI24 values above 60%. From the results of the study, it can be concluded that the isolated bacteria produce EPS that can be investigated in detail for biotechnological applications.