Analysis of Economic Efficiency of Wildlife Law Enforcement in Serengeti Ecosystem Tanzania

Law enforcement remains to be the main strategy used to combat poaching and account for high budget share in protected area management. Studies on efficiency of wildlife law enforcement in the protected areas are limited. This study analyzed economic efficiency of wildlife law enforcement in terms of resource used and output generated using three different protected areas (PAs) of Serengeti ecosystem namely Serengeti National Park (SENAPA), Ikorongo/Grumeti Game Reserves (IGGR) and Ikona Wildlife Management Area (IWMA). Three years (2010-2012) monthly data on wildlife law enforcement inputs and outputs were collected from respective PAs authorities and supplemented with key informant interviews and secondary data. Questionnaire surveys were conducted to wildlife law enforcement staff. Shadow prices for non-marketed inputs were estimated, and market prices for marketed inputs. Data Envelopment Analysis (DEA) was used to estimate economic efficiency using Variable Return to Scale (VRS) and Constant Return to Scale (CCR) assumptions. Results revealed that wildlife law enforcement in all PAs was economically inefficient, with less inefficiency observed in IWMA. The less inefficiency in IWMA is likely attributed to existing sense of ownership and responsibility created through community-based conservation which resulted in to decrease in law enforcement costs. A slacks evaluation revealed a potential to reduce fuel consumption, number of patrol vehicles, ration and prosecution efforts at different magnitudes between studied protected areas. There is equal potential to recruit more rangers while maintaining the resting time. These finding forms the bases for monitoring and evaluation with respect to resource usage to enhance efficiency. It is further recommended to enhance community participation in conservation in SENAPA and IGGR to lower law enforcement costs. Collaboration between protected area, police and judiciary is fundamental to enhance enforcement efficiency. Despite old dataset, these findings are relevant since neither conservation policy nor institution framework has changed substantially in the last decade.

Feeding ecology and interactions with mammal hosts in a symbiotic genus of birds(Buphagus spp.)in Namibia

As the sole obligate symbiotic birds in Africa,oxpeckers offer a unique model for studying symbiotic relationships.Due to the multitrophic level they occupy and the context dependent foraging behavior they exhibit,the type of symbiotic relationship can be variable.In addition to providing a cleaning service to the host by removing ticks,oxpeckers frequently feed on blood,mucus,and saliva,inflicting potential damage on the host.Here,we used DNA metabarcoding on faecal samples to analyze the taxonomic composition of the trophic interactions of the Yellow-billed Oxpecker(Buphagus africanus)and Red-billed Oxpecker(B.erythrorhynchus)in northeastern Namibia.In contrast to conventional methods,DNA metabarcoding allows for a detailed identification of dietary resources encompassing both mammal hosts and consumed arthropods within the same samples.With this information,we examined differences in the diet composition between oxpecker species and localities,as well as the co-occurrence between host and arthropod species.Our findings revealed that oxpeckers predominantly source their diet from mammals,ticks,and flies;however,ticks and flies rarely co-occur in the diet of an individual.We observed variability among individuals in their feeding ecology,which is strongly correlated with locality and,to a lesser extent,with the mammal host.We noted a high degree of mobility between hosts within relatively short periods,with 32%of the samples showing traces of at least two mammal hosts.This study illustrates the dynamic foraging behavior of these specialized symbiotic birds,shedding light on their potential role in pest control services and disease transmission.

Soil erosion susceptibility mapping of Hangu Region,Kohat Plateau of Pakistan using GIS and RS-based models

Soil erosion is a crucial geo-environmental hazard worldwide that affects water quality and agriculture,decreases reservoir storage capacity due to sedimentation,and increases the danger of flooding and landslides.Thus,this study uses geospatial modeling to produce soil erosion susceptibility maps(SESM)for the Hangu region,Khyber Pakhtunkhwa(KPK),Pakistan.The Hangu region,located in the Kohat Plateau of KPK,Pakistan,is particularly susceptible to soil erosion due to its unique geomorphological and climatic characteristics.Moreover,the Hangu region is characterized by a combination of steep slopes,variable rainfall patterns,diverse land use,and distinct soil types,all of which contribute to the complexity and severity of soil erosion processes.These factors necessitate a detailed and region-specific study to develop effective soil conservation strategies.In this research,we detected and mapped 1013 soil erosion points and prepared 12 predisposing factors(elevation,aspect,slope,Normalized Differentiate Vegetation Index(NDVI),drainage network,curvature,Land Use Land Cover(LULC),rainfall,lithology,contour,soil texture,and road network)of soil erosion using GIS platform.Additionally,GIS-based statistical models like the weight of evidence(WOE)and frequency ratio(FR)were applied to produce the SESM for the study area.The SESM was reclassified into four classes,i.e.,low,medium,high,and very high zone.The results of WOE for SESM show that 16.39%,33.02%,29.27%,and 21.30%of areas are covered by low,medium,high,and very high zones,respectively.In contrast,the FR results revealed that 16.50%,24.33%,35.55%,and 23.59%of the areas are occupied by low,medium,high,and very high classes.Furthermore,the reliability of applied models was evaluated using the Area Under Curve(AUC)technique.The validation results utilizing the area under curve showed that the success rate curve(SRC)and predicted rate curve(PRC)for WOE are 82%and 86%,respectively,while SRC and PRC for FR are 85%and 96%,respectively.The validation results revealed that the FR model performance is better and more reliable than the WOE.

An Analysis of Land Use and Land Cover Changes, and Implications for Conservation in Mukumbura (Ward 2), Mt Darwin, Zimbabwe, 2002-2022

Understanding trends of land use land cover (LULC) changes is important for biodiversity monitoring and conservation planning, and identifying the areas affected by change and designing sustainable solutions to reduce the changes. The study aims to evaluate and quantify the historical changes in land use and land cover in Mukumbura (Ward 2), Mt Darwin, Zimbabwe, from 2002 to 2022. The objective of the study was to analyse the LULC changes in Ward 2 (Mukumbura), Mt Darwin, Northern Zimbabwe, for a period of 20 years using geospatial techniques. Landsat satellite images were processed using Google Earth Engine (GEE) and the supervised classification with maximum likelihood algorithm was employed to generate LULC maps between 2002 and 2022 with a five (5) year interval, investigating the following variables, forest cover, barren land, water cover and the fields. Findings revealed a substantial reduction in forest cover by 38.8%, water bodies (wetlands, ponds, and rivers) declined by 55.6%, whilst fields (crop/agricultural fields) increased by 93.3% and the barren land cover increased by 26.3% from 2002 to 2022. These findings point to substantial changes in LULC over the observed years. LULC changes have resulted in habitat fragmentation, reduced biodiversity, and the disruption of ecosystem functions. The study concludes that if these deforestation trends, cultivation, and settlement land expansion continue, the ward will have limited indigenous fruit trees. Therefore, the causes for LULC changes must be controlled, sustainable forest resources use practiced, hence the need to domesticate the indigenous fruit trees in arborloo toilets.

Evaluating the Impacts of Human Activities on Diversity, Abundance, and Distribution of Large Mammals in Nimule National Park, South Sudan

Globally, human activities have a significant impact on the diversity, abundance, and distribution of large mammals in Protected Areas (PAs). These disturbances increase human pressure on biodiversity and species habitats, highlighting the need for conservation. This study aimed to assess the abundance and distribution of large mammals in different habitat types within Nimule National Park (NNP) and understand the impacts of human activities on them. Data on the abundance and distribution of large mammals and their respective habitat types were collected through line transect surveys. Human activity signs were observed and recorded along the transect lines. To estimate the impacts of human activities on the diversity, abundance, and distribution of large mammal species, as well as to identify any significant differences between them and their habitat types, the study utilized the Kruskal Wallis test, Polynomial multiple regressions, and diversity indices. The findings from the Shannon-Weiner and Simpson indices indicated that large mammal species were more diverse inside the park (H’ = 1.136;D = 0.570) compared to the buffer zone (H’ = 0.413;D = 0.171), with 85% (443 out of 510 samples) recorded within Nimule National Park. The species abundance showed a semi-balanced status (0.58). The diversity results among different habitat types revealed that large mammals were more diverse and highly distributed in both open woodlands (244) and dense woodlands (192), while riverine vegetation had the lowest diversity (8). Statistical tests demonstrated a highly significant difference at a 99% confidence interval (p-value = 0.01) between habitat types and identified species of large mammals. Additionally, the results highlighted the high abundance of Uganda kob (274), baboons (141), and warthog (57) across most habitat types, accounting for at least 75% of their distribution. The most prevalent human activities observed were cattle footprints (27%) and cattle dung (14%). Human footprints and tree cutting combined accounted for 9%, indicating the practice of livestock grazing, poaching, encroachment, and fuelwood collection by local communities. However, these activities did not appear to significantly impact the diversity, abundance, and distribution of large mammals in Nimule National Park. Therefore, it is crucial to foster shared responsibilities and engage relevant stakeholders in the management and conservation of large wildlife species. Regular community awareness programs should be implemented to cultivate a sense of ownership. Moreover, it is recommended that a comprehensive survey be conducted on the population status of all mammal species in Nimule National Park, including its surrounding Buffer Zone. Monitoring the impact of human activities on their behavior and habitats using satellite images should also be carried out at least every five to ten years.

Hunting Intensity on Wildlife Population in Oban Sector of Cross River National Park

Hunting intensity in Oban Sector of Cross River National Park, Nigeria was investigated. The methodology involved the recording of signs of hunting activity using line transects, and interviews with hunters. A total of 33 gunshots, 21 spent cartridges, 26 wire snares, presence of 7 hunters and two hunters’ camps and other several hunting signs were recorded for a total of 68 km of transects walked. Nine species of mammals were hunted, notably the Chimpanzee Pan troglodytes, Mona monkey (Cercopithecus mona), Puttynose monkey (Cercopithecus nictitans), Red river hog (Potamochoerus porcus), Drill (Mandrillus leucophaeus), Brush-tailed Porcupine (Atherurus africanus), Blue duiker (Cephalophus monticla), Bushbuck (Tragelaphus scriptus), Ogilby’s duiker (Cephalophus dorsalis), Western tree hyrax (Dendrohyrax dorsalis), and Grasscutter (Thryonomys swinderianus). The interviews revealed the most hunted species as the iii Primates (Cercopithecus spp 28.48%), African brush-tailed Porcupine (Atherurus africanus) (37.74%), Blue Duiker (Philantomba monticola) (26.82%), Bushbuck (Tragelaphus scriptus) (6.96%), Pangolin (Manis tetradactyla), Red river hog (Potamochoerus porcus) and Grasscutter (Thyronomys swinderianus) with the first four being the most commonly hunted. Shotguns and Wire snares were the two main hunting methods used, with the former being used (88.24%) of all the hunters interviewed, and the latter (11.76%) of the hunters. Each hunter set 50 - 300 wire snares, of which there were two types: 1) ground snare without fencing (neck wire snare), and 2) ground snare with fencing (foot/leg wire snares. Most of the wire snares hunting take place mainly during the raining season. The study revealed high hunting intensity in Oban Sector of CRNP. Some of the recommended measures to ameliorate the menace include the initiation of public education and awareness programmes, establishment of task forces to check bushmeat hunting, execution of stricter law enforcement, good welfare package for park rangers and more punitive sanctions for offenders.

Effect of a Wildlife-Livestock Interface on the Prevalence of Intra-Erythrocytic Hemoparasites in Cattle

We conducted a cross-sectional study to establish the effect of proximity of livestock to a wildlife-livestock interface on the relative abundance of intra-erythrocytic hemoparasites in cattle. Blood samples were obtained from 131 randomlyselected cattle raised around Queen Elizabeth National Park. Cattle-farm location was determined by using Global Positioning System device from an arbitrarily reference point. Giemsa-stained blood smears were examined microscopically for intra-erythrocytic hemoparasites. Correlational analysis was used to examine the relationship between farm location and prevalence, whereas risk ratios were used to determine the strength of mixed hemoparasitic infections among cattle, using a significant level of α = 0.05. The location of a cattle farm significantly predicted the prevalence of Anaplasma (rs = 0.33, p 0.05) and Theileria (rs = 0.57, p 0.01) but, farm’s proximity to QENP did not explain the variation in the prevalence of Babesia (rs = 0.14, p 0.2). Although mixed infections occurred in 15% of sampled cattle, concurrent infection of cattle with A. marginale and B. bigemina [RR = 36;95% CI (7.191);p which was recorded. This study demonstrated that unlike the prevalence of B. bigemina, the prevalence of T. parva and A. marginale in livestock significantly increased with close proximity to a wildlife-livestock interface.

The mechanisms of color production in black skin versus red skin on the heads of New World vultures

A determination of how the color of animal integument is produced is a starting point for investigations into the function and evolution of coloration.The mechanisms that give rise to the color of bare skin of New World vultures are largely unexplored.Here,we investigate the source of color production in the bare skin of Turkey Vultures(Cathartes aura)and Black Vultures(Coragyps atratus).Using UV-vis reflectance spectroscopy,we found evidence that hemoglobin is the primary pigment responsible for the red coloration of the bare skin on the heads of Turkey Vultures,and that eumelanin is responsible for the black coloration of the bare skin on the heads of Black Vultures.Light microscopy of incisional skin samples further supported these mechanisms of color production by revealing the presence of numerous blood vessels near the surface of the Turkey Vulture skin,and a high concentration of melanosomes in the skin of Black Vultures.Using high performance liquid chromatography(HPLC),we detected carotenoids within the skin of both species with significantly higher total concentrations of carotenoids in the skin of Turkey Vultures compared to the skin of Black Vultures.The carotenoids detected were dietary carotenoids that typically produce yellow coloration when accumulated in integument and were present in low concentrations.We hypothesize that the dietary carotenoids present do not contribute to the color of the skin,but rather help to compensate for the lack of melanosomes found in Turkey Vulture skin.The presence of additional carotenoids may act as an antioxidant to minimize UV damage when the bare Turkey Vulture head skin is exposed to direct sunlight for prolonged periods of time when soaring and scavenging for food.

Tree-based ecosystem services supply and multifunctionality of church forests and their agricultural matrix near Lake Tana,Ethiopia

Ecosystem services(ES)are the connection between nature and society,and are essential for the well-being of local communities that depend on them.In Ethiopia,church forests and the surrounding agricultural matrix supply numerous ES.However,the ES delivered by both land use types have not yet been assessed simultaneously.Here we surveyed both church forests and their agricultural matrices,aiming to quantify,compare and unravel the drivers underlying tree-based ES supply,density and multifunctionality.We found that almost all church forests and half of the agricultural matrices provided high ES densities.ES multifunctionality was higher in the agricultural matrices,suggesting that people deliberately conserve or plant multifunctional tree species.Furthermore,the supply of all categories of ES was positively correlated with church forest age(p-value<0.001)in the agricultural matrix,while the extent of church forest was positively correlated with the density of all categories ecosystem services score in the church forests(p-value<0.001).Our results can be used to prioritize conservation efforts at sites that provide high levels of ES supply,ES density and ES multifunctionality,and to prioritize restoration efforts at sites with low levels thereof.

The Knowledge about the Potential Health Risks of Illegal Bushmeat Activities among Local Communities Adjacent to Western Nyerere National Park, Tanzania

Establishing knowledge of local communities regarding the potential health risks of illegal bushmeat hunting and its related practices is among the essential means to developing effective conservation and public health programs. To reveal the understanding of the local people regarding the potential health risks of bushmeat consumption and handling of wildlife products, we used a semi-structured questionnaire to survey 261 households in eight villages located adjacent to the western part of Nyerere National Park. Also, we interviewed eight key informants, including conservation personnel and veterinary officers, in the study area. The proportion of local people who were unaware that handling of wildlife products and bushmeat consumption were risky behaviours towards acquiring zoonotic diseases was slightly higher (57%) than the proportion of respondents who were aware of the likely risks of zoonotic diseases from the practices. After all, the majority (83%) of local people admitted having come into contact with wildlife products, while over 70% reported having consumed bushmeat. We found that local communities living closer to the park boundaries (<15 km) have a higher likelihood of contacting wildlife products, and that community members who have lived in the area for more than ten years have experienced more contact with wildlife products than immigrants. Moreover, the age of inhabitants and length of stay were found to be the most significant factors in determining the likelihood of bushmeat consumption. Most people seemed to prefer bushmeat to domestic meat, arguing that bushmeat is far more flavorful, tender, not tainted with chemicals, and has less fat, making it safer. Our study demonstrated that local communities participate in risky practices without contemplating the health consequences that could emerge should a zoonotic disease outbreak occur. Among other strategies, we recommended conservation and public health institutions to increase awareness campaigns on the possible health risks of zoonotic diseases associated with wildlife.

Assessing forest cover changes and fragmentation in the Himalayan temperate region: implications for forest conservation and management

This study comprehensively assessed long-term vegetation changes and forest fragmentation dynamics in the Himalayan temperate region of Pakistan from 1989 to 2019.Four satellite images,including Landsat-5 TM and Landsat-8 Operational Land Imager(OLI),were chosen for subsequent assessments in October 1989,2001,2011 and 2019.The classified maps of 1989,2001,2011 and 2019 were created using the maximum likelihood classifier.Post-classification comparison showed an overall accuracy of 82.5%and a Kappa coefficient of 0.79 for the 2019 map.Results revealed a drastic decrease in closed-canopy and open-canopy forests by 117.4 and 271.6 km^(2),respectively,and an increase in agriculture/farm cultivation by 1512.8 km^(2).The two-way ANOVA test showed statistically significant differences in the area of various cover classes.Forest fragmentation was evaluated using the Landscape Fragmentation Tool(LFT v2.0)between 1989 and 2019.The large forest core(>2.00 km^(2))decreased from 149.4 to 296.7 km^(2),and a similar pattern was observed in medium forest core(1.00-2.00 km^(2))forests.On the contrary,the small core(<1.00 km^(2))forest increased from 124.8 to 145.3 km^(2) in 2019.The perforation area increased by 296.9 km^(2),and the edge effect decreased from 458.9 to 431.7 km^(2).The frequency of patches also increased by 119.1 km^(2).The closed and open canopy classes showed a decreasing trend with an annual rate of 0.58%and 1.35%,respectively.The broad implications of these findings can be seen in the studied region as well as other global ecological areas.They serve as an imperative baseline for afforestation and reforestation operations,highlighting the urgent need for efficient management,conservation,and restoration efforts.Based on these findings,sustainable land-use policies may be put into place that support local livelihoods,protect ecosystem services,and conserve biodiversity.

The Current Conservation Efforts and Future Prospects for the Endangered Nubian Ibex (Capra nubiana ibex) in Sudan

A comprehensive action plan for the conservation of the endangered species, the Nubian ibex in Sudan, can be developed by gaining a thorough understanding of their current status, conservation strategy, and relevant laws and regulations, as well as raising awareness about the importance of protecting endangered species. The Nubian ibex is listed as an endangered species on The International Union for Conservation of Nature (IUCN) Red List, highlighting the need for further research on population conservation efforts due to insufficient population data. To address this knowledge gap, a questionnaire was conducted with various stakeholders, including police officers, researchers, and lecturers, representing a diverse range of organizations and universities. The findings revealed that hunting is the primary factor contributing to endangerment. Mammals account for 80% of endangered species, while reptiles comprise less than one-tenth. Research centers are recognized as the main governing body, and 85% of participants are concerned about the declining population. Hunting accounted for less than half of the threats to the ibex population in Sudan, while habitat loss made up a quarter. Mining, climate change, human activity, and agriculture were also identified as risks. However, there were no plans, strategies, procedures, or measures in place to conserve the Nubian ibex. There were also no initiatives to preserve its biodiversity, and awareness about endangered species was lacking. Although participants believed that laws were effective in protecting the ibex, no licenses were issued for its conservation, and annual surveys were not conducted. Additionally, there were no recorded instances of Mukhalfat related to the Nubian ibex. In light of these findings, we propose various conservation measures to address these challenges. These measures include the implementation of laws and regulations, conducting annual surveys to monitor population trends, protecting habitats, establishing breeding and releasing programs, launching awareness campaigns, undertaking rehabilitation efforts, enhancing research efforts, and developing comprehensive conservation strategies. Additionally, it is crucial to foster cooperation among wildlife institutes to ensure the effective implementation of these conservation measures.

Foraging behavior of the Eurasian Three-toed Woodpecker subspecies Picoides tridactylus funebris in southern Gansu, China

The Three-toed Woodpecker subspecies Picoides tridactylus funebris is endemic to the Qinghai-Tibet plateau in China. No part of its life history was ever reported since its discovery in 1870. To close this gap, we observed foraging behavior of a pair between April and August 2007. A total of 117 observations (28 for male and 89 for female) were obtained by following the birds within their home-ranges using radio-tracking. P. t. funebris preferred foraging on live spruces and snags bigger than available with an average diameter of breast height (DBH) of 32.7 ± 9.2 cm. The most frequent foraging technique was pecking (39.8% of foraging time) and peeling (13.2%). Moreover, sap-sucking was observed more often in P. t. funebris than in P. t. alpinus, suggesting that P. t. funebris was more dependent on the tree sap than the other subspecies. We found distinct niche partitioning between the sexes with respect to use of three out of four investigated parameters of the foraging substrates. These differences were likely related to sexual dimorphism pronounced by slightly larger bill of the male. We concluded that the subspecies P. t. funebris displayed foraging behavior which was very similar to that of other subspecies of the Three-toed Woodpecker.

An initial investigation replacing fish meal with a commercial fermented soybean meal product in the diets of juvenile rainbow trout

The inclusion of PepSoyGen (PSG), a commercially-available fermented soybean meal product, was evaluated with juvenile rainbow trout, Oncorhynchus mykiss in an initial 70-day feeding trial, with a supplemental trial involving a subset of the experimental diets continuing for an additional 40 d. Six diets containing 0%, 10%, 20%, 30%, 40%, or 50% PSG, with the PSG directly replacing fish meal, were used in the first trial. There were no significant differences in weight gain or feed conversion ratio between the fish meal-based control diet and diets containing up to 30% PSG. However, weight gain was significantly reduced and feed conversion ratio significantly increased with the 40% and 50% PSG diets. No health assessment differences were observed in fish receiving any of the diets, and no evidence of gross gut inflammation was evident. There were no significant differences in weight gain or feed conversion ratio among the four dietary treatments ranging from 0% to 30% PSG which were fed for an additional 40 d after the initial 70-d trial (110 days total). Based on these results, juvenile rainbow trout diets can contain up to 30% PSG without any loss of rearing performance, thereby replacing at least 60% of the fish meal.

Comparison of locomotor behaviour between white-headed langurs Trachypithecus leucocephalus and Franois' langurs T. franoisi in Fusui,China

We studied the locomotor behaviour of white-headed langurs Trachypithecus leucocephalus and Francois＇ langurs T.francoisi to test two hypotheses： （1） these monkeys have evolved locomotor ability to support their activities on limestone hills, and （2） Francois＇ langurs have evolved more diverse locomotor skills than white-headed langurs. Data were collected from 1996 - 1998 and in 2005 in Fusui Nature Reserve, Guangxi, and showed that the two species had similar locomotor types, but Francois＇ langurs had more locomotor modes （26） than white-headed langurs （12）. Quadrupedal walking and leaping were two major types, and white-headed langurs were more arboreal than Francois＇ langurs. We suggest that, while keeping their ancestral locomotor types, the two species have evolved new types allowing them to live on limestone cliffs. Compared to white-headed langurs, Francois＇ langurs have more diverse locomotor modes that probably have allowed them to live in more habitat types. As an evolutionary outcome, Francois＇ langurs have a larger distribution range .

Population Variation and Food Habits of Ranid Frogs in the Rice-Based Cropping System in Gujranwala Region,Pakistan

The current study aims to investigate the population variation and food habits of ranid frogs in the rice-based cropping system in District Gujranwala,Pakistan.The population in the study area was estimated using capture,mark and release method whereas food habits of the species were studied by analysis of stomach contents.The results showed the highest average population was found during August 2009(93.10±18.64/ha) while the lowest from December 2008 to February 2009.Maximum seasonal populations existed in summer 2009,whereas winter 2008 sizes were at a minimum.Stomach content analysis of the species revealed percent frequency(% F) of occurrence of insects(80.3),earthworms(28.5),whole frogs(15.8),bone pieces(22.5),rodents(1.66),vegetation(5.0),soil particles(13.3) and some unidentified material(7.5) in all the stomach samples.Most frequently consumed prey items were insects(30% by volume),although frogs also preyed upon conspecifics and rodents.Insects recovered from the stomach contents were identified as belonging to Orthoptera,Lepidoptera,Coleoptera,Diptera,Odonata and Homoptera as well as the class Archnida.Insects recovered from the stomach contents were compared to those captured from the study area.

Demography and dynamics of mountain nyala Tragelaphus buxtoni in the Bale Mountains National Park,Ethiopia

We studied the population dynamics of endangered mountain nyala Tragelaphus buxtoni between 2003-2005 in the Bale Mountains National Park. Line-transect sampling and total count methods were used to gather data on demographics and movement patterns. The population＇s age-group composition was 58% adults, 25% sub-adults, 9% juveniles, 5% calves and 3% unidentified with a female-male sex ratio of 2：1. Population density was found to be significantly different between the two sub-populations （Dinsho Sanctuary and Gaysay/Adelay）. A significant difference was found for age-group composition across the two sub-populations except adult females, sub-adult males and calves. The Dinsho sub-population was an isolated group. Separation and containment of the mountain nyala population could have negatively affected their ability to search for habitat requirements and mates from distant areas. The population varied between 830-908 individuals （95% CI）, a reduction of 45% from earlier reports. However, the mean population density increased due to contraction of the species＇ habitat range. We observed a population decrease of 2%-5% per year over the course of our study. Many of the assessed demographic parameters did not significantly change over the three years. This suggests that the decrease in nyala population was not due to random variations in reproduction. Anthropogenic factors such as competition with livestock for forage, habitat encroachment and poaching by the local people might have been partly responsible for the depleted population in our study areas [Current Zoology 56 （6）： 660q569, 2010].

Plants Diversity of the Burigi-Chato National Park: Rare and Invasive Species

This study was conducted within a distance of three to five kilometres around Lake Burigi as a study area in the Burigi-Chato National Park for the purpose of documenting the diversity of plants, identifying invasive and rare species for enhancing conservation in the park. Lake Burigi was purposively sampled as a study area for plant identification due to its potential as a tourist destination. No study has been conducted in the study area to reveal plants diversity, with a focus on documenting invasive and rare species, thus necessitating undertaking of this study to inform the park authority to take urgent control measures in avoiding widespread of invasive species and proper planning for conservation of rare species. The methods involved included field observation;plant identification using field guide books, indigenous and professional knowledge;field mapping using GPS receiver and literature review. Analysis of meteorological data and soil sample were also used among other methods. Meteorological data were analysed using Microsoft Excel and SPSS v.20 to determine the relationship between variables. A total of 102 plant species of different growth forms of trees, shrubs, grasses, sedges, forbs, and herbs were identified and recorded. Tegetes minuta and Argemone mexicana were recorded as invasive plant species, while six rare plant species potential for pharmaceutical industry;Zanthoxylum usambarense, Gardenia ternifolia, Faidherbia albida, Harrisonia abyssinica, Anona senegalensis, and, Pappea capensis were also recorded. Scars of wildfires were observed in the study area. The study area received an average rainfall of 964.36 mm per year, the highest peak recorded in 1951. The trend of rainfall showed that many years had rainfall below the average while the temperature was found to increase from year to year, the situation which suggests the existence of climate change in the study area. There was a weak negative relationship between temperature and wind speed. Soil nutrients and disturbances in the area were found to favour the growth of Tegetes minuta. Findings of this study would help ecological unit in the park to conduct regular ecological assessment for the purpose of controlling invasive plant species, which if left to flourish are likely to reduce habitat suitability for ungulates to utilize the area. Moreover, knowing which plant species are rare, adds value to the area as a destination to visitors interested in plants. The findings also allow the park authority to keep record of species rarity and thus easily take control of them to avoid unintended exploitation. Based on the findings, it was concluded that a number of disturbances, meteorological and edaphic factors favour the growth of invasive species. Urgent measures are to be taken to control the observed invasive plant species before they are left to spread in the park to avoid destruction of the habitat suitability and incurring unnecessary expenses and time in combating them. Furthermore, rare plant species especially those with pharmaceutical and industrial values are to be conserved with special attention to ensure their continued survival. It was recommended that human-induced activities should be prevented through regular ecological assessment, field patrols and public awareness programs. A similar study needs to be conducted during dry season to make comparison of the capacity of plants in adapting to different changes of the weather.

Butterfly Species Diversity and Abundance in University of Ibadan Botanical Garden, Nigeria

Understanding the significance of butterflies in an ecosystem as an environmental healthy indicator and pollination of flowering plants is crucial to achieving sustainability and conservation of floral diversity. The aim of the study was to investigate the butterfly species diversity and abundance and compare the relationship between physical factor and butterfly species. Line transect were used to survey three habitats around botanical garden with scooped-net between June and July, 2010. Diversity varied from habitat to habitat. A checklist was made comprising a total of 57 butterfly species that were surveyed belonging to 9 families. There was a relationship between temperature and butterfly species diversity and abundance. There was no correlation between physical factors (relative humidity and temperature) and the total number of species and families. Moreover, butterfly numbers were not related to relative humidity in any species and family. However, temperature was significantly correlated with species. Therefore, understanding of the factors that affect butterfly species diversity and abundance in University of Ibadan Botanical Garden is important for conservation.

In vitro performance and in vivo fertility of antibiotic-free preserved boar semen stored at 5℃

Background: Hypothermic preservation of boar semen is considered a potential method for omitting antibiotics from insemination doses, thereby contributing to the global antibiotic resistance defence strategy. The main challenges are chilling injury to spermatozoa and bacterial growth during semen storage leading to reduced fertility.Objectives: To examine chilling injury and the number and type of bacteria in boar semen stored at 5 ℃ in the absence of antibiotics, and to assess the applicability of hypothermic semen storage under field conditions.Material and methods: Boar ejaculates were extended with AndroStar~? Premium, stored at 17 ℃ with and at 5 ℃ without antibiotics and tested for functional sperm parameters by flow cytometry. Raw semen and extended samples were investigated bacteriologically. Fertility was evaluated after once-daily inseminations of 194 sows in a field study.Results: Lethal sperm damage assessed by motility and membrane integrity was low throughout storage in both experimental groups. Sublethal chilling effects based on the decrease of viable spermatozoa with low membrane fluidity were higher(P < 0.05) up until 72 h in sperm stored at 5 ℃ compared to 17 ℃ but did not differ after 144 h.After 72 h, incubation in capacitating medium for 60 min induced a similar decrease in viable sperm with high mitochondria membrane potential and low cytosolic calcium in both groups. In semen stored at 5 ℃, bacteria counts were below 103 CFU/mL and the bacteria spectrum was similar to that of raw semen. In 88% of 34 boars,cooled semen fulfilled the requirements for insemination. Fertility was high and did not differ(P > 0.05) between sow groups inseminated with semen stored antibiotic-free at 5 ℃ and semen stored at 17 ℃ with antibiotics.Conclusion: Despite subtle chilling effects and low bacterial numbers, antibiotic-free hypothermic storage of boar semen offers the possibility to reduce the use of antibiotics in pig insemination. However, strict sanitary guidelines must be maintained and further evidence of efficiency under field conditions is considered desirable.