A 3-year-old boy presented with postprandial vomiting and epigastric pain for 3 wk. Barium meal study suggested hypertrophic pyloric stenosis. Ultrasound of the stomach after water loading revealed an echogenic antral...A 3-year-old boy presented with postprandial vomiting and epigastric pain for 3 wk. Barium meal study suggested hypertrophic pyloric stenosis. Ultrasound of the stomach after water loading revealed an echogenic antral web with an eccentric aperture and distal antral hypertrophy.Subsequent endoscopy confirmed the ultrasound findings.Web resection and antropyloroplasty resulted in excellent recovery. To our knowledge, the barium meal and ultrasound findings of an antral web-associated distal antral hypertrophy and prepyloric stenosis has not previously been described.展开更多
Interleukin (IL)-8 is a potent neutrophil chemotactic factor and a crucial mediator in neutrophil-dependent inflammation.Various cell types produce IL-8, either in response to external stimuli such as cytokines or bac...Interleukin (IL)-8 is a potent neutrophil chemotactic factor and a crucial mediator in neutrophil-dependent inflammation.Various cell types produce IL-8, either in response to external stimuli such as cytokines or bacterial infection, or aftermalignant transformation. Anti-IL-8 strategies have been considered for anti-inflammatory therapy. In this paper wedemonstrate that the RNA interference technique can be used to efficiently down-regulate IL-8 protein expression inairway epithelial cells. We used a helper-dependent adenoviral vector to express a small hairpin (sh)RNA targetinghuman IL-8 in cultured airway epithelial cells (IB3-1, Cftr-/-; C38, Cftr-corrected) stimulated with TNF-α, IL-1β orheat-inactivated Burkholderia cenocepacia. Stimulated IL-8 expression in IB3-1 and C38 cells was significantly reducedby shRNA expression. The shRNA targeting IL-8 had no effect on the activation of NF-κB, or on the protein levels ofIκB or IL-6, suggesting that this anti-IL-8 strategy was highly specific, and therefore may offer potential for thetreatment of inflammatory diseases.展开更多
背景：青少年肥胖正在成为一个全国性的公共健康问题。包括西布曲明(sibutramine)在内的减肥药物不但有利于成人的体重控制而且可将其与行为治疗(behavior therapy，BT)结合用于青少年肥胖。目的：在一项以家庭为基础的行为体重控制计...背景：青少年肥胖正在成为一个全国性的公共健康问题。包括西布曲明(sibutramine)在内的减肥药物不但有利于成人的体重控制而且可将其与行为治疗(behavior therapy，BT)结合用于青少年肥胖。目的：在一项以家庭为基础的行为体重控制计划中，检验加用西布曲明是否可以提高青少年肥胖的减肥效果。设计、地点和参加者：于1999年3月至2002年8月在一大学诊所进行的随机双盲、安慰剂对照试验。82例参试青少年的年龄13—17岁，体重指数(body mass index，BMI)32—44。先治疗6个月，然后开放标记(简称：开标，open—label)继续治疗7—12个月。干预：头6个月，参试者或者接受BT加西布曲明或者接受BT加安慰剂。从第7-第12个月，所有参试者均接受开标西布曲明治疗。主要观察指标：BMI变化百分率；收缩压、舒张压、脉搏以及饥饿(感)。结果：在6个月的意向-治疗分析中，BT加西布曲明组平均(SD)体重减轻7．8kg(6．3kg)，BMI下降8．5％(6．8％)，显著大于BT加安慰剂组的3．2kg(6．1kg)和4．0％(5．4％)。接受西布曲明的参试者报告饥饿感亦显著减轻(P=0．002)。第7～12个月，最初用西布曲明治疗的青少年，继续使用该药，体重上升0．8kg(10．5kg)；停用安慰剂改服西布曲明的青少年体重进一步减轻1．3kg(5．4kg)。减少药物剂量(n=23)或者停用(n=10)以控制血压、心率或其他症状的加重。结论：在综合行为治疗计划中，加上西布曲明可导致较行为治疗加安慰剂更为显著的体重减轻。在获得更广泛的安全性和有效性数据前，减肥药只宜在试验的基础上用于儿童和青少年。展开更多
文摘A 3-year-old boy presented with postprandial vomiting and epigastric pain for 3 wk. Barium meal study suggested hypertrophic pyloric stenosis. Ultrasound of the stomach after water loading revealed an echogenic antral web with an eccentric aperture and distal antral hypertrophy.Subsequent endoscopy confirmed the ultrasound findings.Web resection and antropyloroplasty resulted in excellent recovery. To our knowledge, the barium meal and ultrasound findings of an antral web-associated distal antral hypertrophy and prepyloric stenosis has not previously been described.
文摘Interleukin (IL)-8 is a potent neutrophil chemotactic factor and a crucial mediator in neutrophil-dependent inflammation.Various cell types produce IL-8, either in response to external stimuli such as cytokines or bacterial infection, or aftermalignant transformation. Anti-IL-8 strategies have been considered for anti-inflammatory therapy. In this paper wedemonstrate that the RNA interference technique can be used to efficiently down-regulate IL-8 protein expression inairway epithelial cells. We used a helper-dependent adenoviral vector to express a small hairpin (sh)RNA targetinghuman IL-8 in cultured airway epithelial cells (IB3-1, Cftr-/-; C38, Cftr-corrected) stimulated with TNF-α, IL-1β orheat-inactivated Burkholderia cenocepacia. Stimulated IL-8 expression in IB3-1 and C38 cells was significantly reducedby shRNA expression. The shRNA targeting IL-8 had no effect on the activation of NF-κB, or on the protein levels ofIκB or IL-6, suggesting that this anti-IL-8 strategy was highly specific, and therefore may offer potential for thetreatment of inflammatory diseases.
文摘背景：青少年肥胖正在成为一个全国性的公共健康问题。包括西布曲明(sibutramine)在内的减肥药物不但有利于成人的体重控制而且可将其与行为治疗(behavior therapy，BT)结合用于青少年肥胖。目的：在一项以家庭为基础的行为体重控制计划中，检验加用西布曲明是否可以提高青少年肥胖的减肥效果。设计、地点和参加者：于1999年3月至2002年8月在一大学诊所进行的随机双盲、安慰剂对照试验。82例参试青少年的年龄13—17岁，体重指数(body mass index，BMI)32—44。先治疗6个月，然后开放标记(简称：开标，open—label)继续治疗7—12个月。干预：头6个月，参试者或者接受BT加西布曲明或者接受BT加安慰剂。从第7-第12个月，所有参试者均接受开标西布曲明治疗。主要观察指标：BMI变化百分率；收缩压、舒张压、脉搏以及饥饿(感)。结果：在6个月的意向-治疗分析中，BT加西布曲明组平均(SD)体重减轻7．8kg(6．3kg)，BMI下降8．5％(6．8％)，显著大于BT加安慰剂组的3．2kg(6．1kg)和4．0％(5．4％)。接受西布曲明的参试者报告饥饿感亦显著减轻(P=0．002)。第7～12个月，最初用西布曲明治疗的青少年，继续使用该药，体重上升0．8kg(10．5kg)；停用安慰剂改服西布曲明的青少年体重进一步减轻1．3kg(5．4kg)。减少药物剂量(n=23)或者停用(n=10)以控制血压、心率或其他症状的加重。结论：在综合行为治疗计划中，加上西布曲明可导致较行为治疗加安慰剂更为显著的体重减轻。在获得更广泛的安全性和有效性数据前，减肥药只宜在试验的基础上用于儿童和青少年。
