Improving dryland maize productivity and water efficiency with heterotrophic ammonia-oxidizing bacteria via nitrification and cytokinin activity

A two-year field experiment conducted under dryland conditions in semi-humid and drought-prone regions of China aimed to assess the effect of ammonia-oxidizing bacterial on maize water use efficiency and yield.A heterotrophic ammonia-oxidizing bacteria(HAOB)strain S2_8_1 was used.Six treatments were applied:(1)no irrigation+HAOB strain(DI),(2)no irrigation+blank culture medium(DM),(3)no irrigation control(DCK),(4)irrigation+HAOB(WI),(5)irrigation+blank culture medium(WM),and(6)irrigation control(WCK).Results revealed that HAOB treatment increased maize growth,yield,and water use efficiency over controls,regardless of whether the year was wet or dry.This improvement was attributed to the accelerated nitrification in the rhizosphere soil due to HAOB inoculation,which subsequently led to increased levels of leaf cytokinins.Overall,these findings suggest that HAOB inoculation holds promise as a strategy to boost water use efficiency and maize productivity in dryland agriculture.

Drip Irrigation Scheduling for Tomato Grown in Solar Greenhouse Based on Pan Evaporation in North China Plain

This study has investigated the suitable drip irrigation scheduling for tomato grown in solar greenhouse based on 20-cm pan evaporation （Epan） in North China Plain. Irrigation treatments included three irrigation frequencies （11 10, 12 20 and 13 30 mm, and irrigation interval of 2-6 d for 11, 4-9 d for 12 and 8-12 d for 13） based on accumulated pan evaporation （Epan）, and four plant-pan coefficients （Kcp1 0.5, Kcp2 0.7, Kcp3 0.9 and Kcp4 1.1）. Results indicate that total irrigation amount, seasonal crop evapotranspiration （ET） and tomato yield （Y） were 185.1-365.8 mm, 249.1-388.0 mm and 99.6-151.8 t ha^-1, respectively. Irrigation frequency and amount increased the yield, and second-degree polynomial relationship was found between Y and ET （R2=0.8671）. Irrigation frequency did not increase mean fruit weight, diameter and length significantly but increased fruit number, total soluble solids content （TSS）, TSS yield, fruit firmness and water use efficiency （WUE） and irrigation WUE （IWUE） significantly. Irrigation amount increased external quality of tomato but reduced TSS content, TSS yield, fruit firmness, WUE and IWUE significantly. Kep3 and gep4 treatments had the highest fruit yield, but Kep2 and Kep3 treatments had the highest WUE. 11Kcp3 treatment （irrigation interval of 2-6 d, and Kop=0.9） had higher IWUE, WUE, external quality, yield, and TSS yield, so it is recommended as the suitable irrigation scheduling for tomato grown in solar greenhouse in North China Plain.

Effects of reclaimed water irrigation and nitrogen fertilization on the chemical properties and microbial community of soil

The ecological effect of reclaimed water irrigation and fertilizer application on the soil environment is receiving more attention.Soil microbial activity and nitrogen（N）levels are important indicators of the effect of reclaimed water irrigation on environment.This study evaluated soil physicochemical properties and microbial community structure in soils irrigated with reclaimed water and receiving varied amounts of N fertilizer.The results indicated that the reclaimed water irrigation increased soil electrical conductivity（EC）and soil water content（SWC）.The N treatment has highly significant effect on the ACE,Chao,Shannon（H）and Coverage indices.Based on a 16S ribosomal RNA（16S rRNA）sequence analysis,the Proteobacteria,Gemmatimonadetes and Bacteroidetes were more abundant in soil irrigated with reclaimed water than in soil irrigated with clean water.Stronger clustering of microbial communities using either clean or reclaimed water for irrigation indicated that the type of irrigation water may have a greater influence on the structure of soil microbial community than N fertilizer treatment.Based on a canonical correspondence analysis（CCA）between the species of soil microbes and the chemical properties of the soil,which indicated that nitrate N（NO3-–-N）and total phosphorus（TP）had significant impact on abundance of Verrucomicrobia and Gemmatimonadetes,meanwhile the p H and organic matter（OM）had impact on abundance of Firmicutes and Actinobacteria significantly.It was beneficial to the improvement of soil bacterial activity and fertility under 120 mg kg^-1 N with reclaimed water irrigation.

Growth and nitrogen productivity of drip-irrigated winter wheat under different nitrogen fertigation strategies in the North China Plain

Excessive application of nitrogen (N) fertilizer is the main cause of N loss and poor use efficiency in winter wheat (Triticum aestivum L.) production in the North China Plain (NCP).Drip fertigation is considered to be an effective method for improving N use efficiency and reducing losses,while the performance of drip fertigation in winter wheat is limited by poor N scheduling.A two-year field experiment was conducted to evaluate the growth,development and yield of drip-fertigated winter wheat under different split urea (46%N,240 kg ha^(-1)) applications.The six treatments consisted of five fertigation N application scheduling programs and one slow-release fertilizer (SRF) application.The five N scheduling treatments were N0–100 (0%at sowing and 100%at jointing/booting),N25–75 (25%at sowing and 75%at jointing and booting),N50–50(50%at sowing and 50%at jointing/booting),N75–25 (75%at sowing and 25 at jointing/booting),and N100–0 (100%at sowing and 0%at jointing/booting).The SRF (43%N,240 kg ha^(-1)) was only used as fertilizer at sowing.Split N application significantly (P<0.05) affected wheat grain yield,yield components,aboveground biomass (ABM),water use efficiency(WUE) and nitrogen partial factor productivity (NPFP).The N50–50 and SRF treatments respectively had the highest yield(8.84 and 8.85 t ha^(-1)),ABM (20.67 and 20.83 t ha^(-1)),WUE (2.28 and 2.17 kg m^(-3)) and NPFP (36.82 and 36.88 kg kg^(-1)).This work provided substantial evidence that urea-N applied in equal splits between basal and topdressing doses compete economically with the highly expensive SRF for fertilization of winter wheat crops.Although the single-dose SRF could reduce labor costs involved with the traditional method of manual spreading,the drip fertigation system used in this study with the N50–50 treatment provides an option for farmers to maintain wheat production in the NCP.

Nitrous oxide emissions following seasonal freeze-thaw events from arable soils in Northeast China

Seasonal soil freeze-thaw events may enhance soil nitrogen transformation and thus stimulate nitrous oxide （N2O） emissions in cold regions. However, the mechanisms of soil N2O emission during the freeze-thaw cycling in the field remain unclear. We evaluated N2O emissions and soil biotic and abiotic factors in maize and paddy fields over 20 months in Northeast China, and the structural equation model （SEM） was used to determine which factors affected N2O production during non-growing season. Our results verified that the seasonal freeze-thaw cycles mitigated the available soil nitrogen and carbon limitation during spring thawing period, but simultaneously increased the gaseous N2O-N losses at the annual time scale under field condition. The N2O-N cumulative losses during the non-growing season amounted to 0.71 and 0.55 kg N ha 1 for the paddy and maize fields, respectively, and contributed to 66 and 18% of the annual total. The highest emission rates （199.2- 257.4 μg m-2 h-1） were observed during soil thawing for both fields, but we did not observe an emission peak during soil freezing in early winter. Although the pulses of N2O emission in spring were short-lived （18 d）, it resulted in approximately 80% of the non-growing season N2O-N loss. The N2O burst during the spring thawing was triggered by the combined impact of high soil moisture, flush available nitrogen and carbon, and rapid recovery of microbial biomass. SEM analysis indicated that the soil moisture, available substrates including NH4＋ and dissolved organic carbon （DOC）, and microbial biomass nitrogen （MBN） explained 32, 36, 16 and 51% of the N2O flux variation, respectively, during the non-growing season. Our results suggested that N2O emission during the spring thawing make a vital contribution of the annual nitrogen budget, and the vast seasonally frozen and snow-covered croplands will have high potential to exert a positive feedback on climate change considering the sensitive response of nitrogen biogeochemical cycling to the freeze-thaw disturbance.

Effects of different LEDs light spectrum on the growth,leaf anatomy,and chloroplast ultrastructure of potato plantlets in vitro and minituber production after transplanting in the greenhouse

Light spectrum plays an important role in regulating the growth and development of in vitro cultured potato(Solanum tuberosum L.) plantlets. The status of potato plantlets at the end of in vitro stage influences the minituber production after transplanting. With 100 μmol m^-2s^-1 total photosynthetic photon flux density(PPFD), a light spectrum study of 100% red light emitting diodes(LEDs) light spectrum(RR), 100% blue LEDs light spectrum(BB), 65% red+35% blue LEDs light spectrum(RB), and 45% red+35% blue+20% green LEDs light spectrum(RBG) providing illumination at the in vitro cultured stage of potato plantlets for 4 weeks using fluorescent lamp as control(CK) was performed to investigate the effects of LEDs light spectrum on the growth, leaf anatomy, and chloroplast ultrastructure of potato plantlets in vitro as well as the minituber yield after 2 months transplanting in the greenhouse. Compared to CK, RB and RBG promoted the growth of potato plantlets in vitro with increased stem diameter, plantlet fresh weight, plantlet dry weight, and health index. Furthermore, BB induced the greatest stem diameter as well as the highest health index in potato plantlets in vitro. Root activity, soluble protein, and free amino acid were also significantly enhanced by BB, whereas carbohydrates were improved by RR. In addition, thickness of leaf, palisade parenchyma and spongy parenchyma was significantly increased by BB and RBG. Chloroplasts under BB and RBG showed well-developed grana thylakoid and stroma thylakoid. Unexpectedly, distinct upper epidermis with greatest thickness was induced and palisade parenchyma and spongy parenchyma were arranged neatly in RR. After transplanting in the greenhouse for 2 months, potato plantlets in vitro from BB, RB, and RBG produced high percentage of large size tuber. BB improved fresh and dry weights of the biggest tuber but decreased tuber number per plantlet. In addition, RBG increased tuber number as well as tuber fresh and dry weight slightly. Our results suggested monochromatic blue LEDs as well as combined red, blue or/and green LEDs light spectrum were superior to fluorescent lamp spectrum in micro-propagation of potato plantlets. Therefore, the application of RBG was suitable;BB and RB could be used as alternatives.

Suitability of Stem Diameter Variations as an Indicator of Water Stress of Cotton

Water stress effects on stem diameter variations （SDV） were studied in a pot experiment on cotton （Gossypium hirustum L. Meimian99B）. Water restriction was imposed at the flowering stage and were compared with a well-watered control treatment. The volumetric soil water content （0v） and SDV were monitored continuously. The objective was to determine the feasibility of using the parameters derived from stem diameter measurements, including maximum daily stem shrinkage （MDS）, maximum daily stem diameter （MXSD）, and minimum daily stem diameter （MNSD） as indicators of plant water stress. The different behavior of SDV was founded at different growth stages. At stem-maturing stage, MDS increased and MNSD decreased in deficit-irrigated plants compared with the control plants, therefore, it appeared that MDS and MNSD ccould be used as available indicators of plant water status. At stem growth stage, there were no significant differences in MDS values between treatments but MXSD and MNSD responded sharply to soil water deficits. Thus, for rapidly growing cotton, the course of MXSD or MNSD with time offered a consistent stress indicator. SDV was also closely related to atmospheric factors, solar radiation （Rs） and vapor pressure deficit （VPD） were found to be the predominant factors affecting MDS, followed by the relative humidity （RH）, while air temperature （Ta） and wind velocity had the least effect. A good linear relationship was founded （r^2 = 0.921） between MDS and environmental variables （Rs, VPD, RH, and θv）, which can be used to establish a reference value for detecting plant water stress based on the MDS patterns.

A proposed surface resistance model for the Penman-Monteith formula to estimate evapotranspiration in a solar greenhouse

Greenhousing is a technique to bridge season gap in vegetable production and has been widely used worldwide. Calculation of water requirement of crops grown in greenhouse and determination of their irrigation schedules in arid and semi-arid regions are essential for greenhouse maintenance and have thus attracted increased attention over the past decades. The most common method used in the literature to estimate crop evapotranspiration（ET） is the Penman-Monteith（PM） formula. When applied to greenhouse, however, it often uses canopy resistance instead of surface resistance. It is understood that the surface resistance in greenhouse is the result of a combined effect of canopy restriction and soil-surface restriction to water vapor flow, and the relative dominance of one restriction over another depends on crop canopy. In this paper, we developed a surface resistance model in a way similar to two parallel resistances in an electrical circuit to account for both restrictions. Also, considering that wind speed in greenhouse is normally rather small, we compared three methods available in the literature to calculate the aerodynamic resistance, which are the r_a^1 method proposed by Perrier（1975a, b）, the r_a^2 method proposed by Thom and Oliver（1977）, and the r_a^3 method proposed by Zhang and Lemeu（1992）. We validated the model against ET of tomatoes in a greenhouse measured from sap flow system combined with micro-lysimeter in 2015 and with weighing lysimeter in 2016. The results showed that the proposed surface resistance model improved the accuracy of the PM model, especially when the leaf area index was low and the greenhouse was being irrigated. We also found that the aerodynamic resistance calculated from the r_a^1 and r_a^3 methods is applicable to the greenhouse although the latter is slightly more accurate than the former. The proposed surface resistance model, together with the r_a^3 method for aerodynamic resistance, offers an improved approach to estimate ET in greenhouse using the PM formula.

The Fabrication of Bio-ceramsite for the Removal of Heavy Metals and Its Toxicity to Bacteria

Bio-ceramsite technology is one of the most effective technologies in the pretreatment of drinking water. In this work, bio-ceramsite was fabricated by Citrobacterfreundii （C. freundii） immobilization on the ceramsite. The findings of the current study suggest that the bio-ceramites showed biosorption abilities for Cd（II） and Pb（II） and the removal efficiency for Pb（II） is lower than Cd（II）. The adsorption mechanism can be attributed to electrostatic attraction and covalent bond. The morphology of the cells changed after the adsorption of Cd（II） and Pb（II） due to the dissociation of the assembly of peptidoglycan and lipopolysaccharide. The fluorescence polarization has shown a significant decrease in membrane fluidity and an increase of permeability of cell membrane. The spectral profile of C. freundii suggests the alteration of carbonyl, amide and phosphonic groups on the cell membrane.

Study on Detection of Pesticide Residues on Winter Jujube Surface by Near-infrared Spectroscopy Combined with PLS and SPA

With fresh winter jujube from the southern region of Xinjiang as the object of study, the method for detecting pesticide residues on winter jujube surface by near-infrared spectroscopy （NIR） combined with successive projections algorithm （SPA） and partial least squares （PLS） was investigated. The absorbance information of winter jujube sample surface was obtained through NIR technology, for the building of a full-wave band PLS model of fresh winter jujube sample sprayed with different concentrations of pesticide （taking chlorpyrifos as an example） as well as an SPA-PLS model which was built with the characteristic wavelengths extracted with SPA as the input variables for PLS, and the prediction precision of the two kinds of models was compared. The model built with the five characteristic waveslengths extected by SPA method only used the variables 0.32% of all the variables in the full wave band, but its accuracy and precision were better than the model built with the full wave band. It is feasible to build a model for different concentrations of chlorpyrifos on fresh winter jujube surfarce by NIR technology combined with SPA and PLS, and SPA method could simplify the complexity of the model and improve the precision and stablity of the model.

An Efficient Multiple-Dimensional Finite Element Solution for Water Flow in Variably Saturated Soils

Multiple-dimensional water flow in variably saturated soils plays an important role in ecological systems such as irrigation and water uptake by plant roots; its quantitative description is usually based on the Richards＇ equation. Because of the nonlinearity of the Richards＇ equation and the complexity of natural soils, most practical simulations rely on numerical solutions with the nonlinearity solved by iterations. The commonly used iterations for solving the nonlinearity are Picard and Newton methods with the former converging at first-order rate and the later at second-order rate. A recent theoretical analysis by the authors, however, revealed that for solving the diffusive flow, the classical Picard method is actually a chord-Newton method, converging at a rate faster than first order; its linear convergence rate is due to the treatment of the gravity term. To improve computational efficiency, a similar chord-Newton method as for solving the diffusive term was proposed to solve the gravity term. Testing examples for one-dimensional flow showed significant improvement. The core of this method is to produce a diagonally dominant matrix in the linear system so as to improve the iteration-toiteration stability and hence the convergence. In this paper, we develop a similar method for multiple-dimensional flow and compare its performance with the classical Picard and Newton methods for water flow in soils characterised by a wide range of van Genuchten parameters.

Detection of Chlorpyrifos Residues in Akesu Red Fuji Apple based on Near Infrared Spectrum

[Objective] The paper was to explore a method for detecting chlorpyrifos residues in red Fuji apple. [Method] The original spectral data of apple samples sprayed with different volume fractions of chlorpyrifos were collected using near infrared spectrometer at the band of 4 000-10 000 cm^（-1）. The original spectra were pre-treated by a variety of methods, and partial least squares（PLS） model was established for predictive analysis. [Result] Near infrared spectrum showed sensitivity to apple samples sprayed with different volume fractions of chlorpyrifos, but had low precision on pesticide-free samples. Data of blank control group were further eliminated for modeling prediction. The results showed that the results were the best when pre-treated with first derivative（FD）： R=0.987 9; the square error of prediction（SEP） was 0.173 6; the root-mean-square error of cross-validation（RMSECV） was 0.120 5; and the precision was 0.923 4. [Conclusion] Near infrared spectrum can better predict chlorpyrifos residue, providing a new method for detecting chlorpyrifos residues in Akesu red Fuji apple.

Wind Field Flow Characteristics Analysis of T4-72 Type Centrifugal Fan

In order to explore the internal wind field flow characteristics of T4-72 type centrifugal fan, the three-dimensional model was established based on PRO/E software. Combined with computational fluid Dynamics Software Fluent 6.3, the standard model and SIMPLEC algorithm were used to simulate the wind field inside the fan. Analysis of the flow characteristics, velocity distributed and pressure distributed of the internal fluid model of the T4-72 centrifugal fan, combined with the theoretical formula to obtain the full pressure, power and efficiency performance parameters of the fan. The centrifugal fan performance curve is drawn. While compared with the experimental data, it is found that the internal flow disturbance is strong when the fan is running under low load condition and high load condition, which affects the performance of the fan and reduces the life of the fan. The numerical simulation results are consistent with the experimental results. The overall performance parameters of the fan are in good agreement, verifying the reliability of the simulation results;when the fan works between 1 - 1.4 times the rated flow rate, it can obtain a more stable flow field while maintaining higher efficiency, which provides a new idea for the optimization of the subsequent fan.

Agricultural water-saving potential and feasibility of developing semi-dryland farming in Henan Province

Based on the collected data in the current status of developing and utilizing water resources and imple- menting water-saving agriculture in Henan Province, and taking into account the influence of engineering, agro- nomic and management measures, the water-saving potential in past years and the feasibility of implementing semi-dryland farming were analyzed in Henan Province. Finally, specific technical measures of developing semi- drvland farming in different areas of Henan Province were orooosed.

Effect of Water Resources Allocation on Groundwater Environment and Soil Salinity Accumulation under Climate Change

The combined surface and groundwater allocation practice by wells and canals had contributed to the safety of groundwater environment and agriculture sustainable production. The typical area in the People＇s Victory Canal irrigation district was taken as a case, drawing together the irrigation district agriculture water consumption and precipitation from 1954 to 2014 in the People＇s Victory Canal irrigation district, ratios of surface to groundwater irrigation amount, dynamic of groundwater depth and hydrochemical characteristic of groundwater from 2008 to 2014 in the research area, the relationship between groundwater depth and ratio of surface to groundwater irrigation amount was analyzed, in order to ascertain the influence of precipitation on ratios of surface to groundwater irrigation amount and its effect on soil and groundwater environment. The results indicated that positive correlation between the ratios of surface to groundwater irrigation amount and annual precipitation was appeared, affected by climate change, average irrigation amount from surface in the recent 5 years was 2.90 x 108 cubic meters, accounted for 75.52% of total irrigation amount, on the other hand, decreasing tendency of precipitation was obvious, and groundwater depth dynamic in upstream of the branch canals was more dramatic than downstream because of surface water irrigation infiltration, under the unified condition of water use efficiency, ratio of surface to groundwater irrigation amount was negative correlation with area of the groundwater depth beyond 11 m, meanwhile, groundwater depth demonstrated negative correlation with the ratio of surface to groundwater irrigation amount, moreover, alkaline trend of groundwater hydrochemistry during the normal season in the research area was obvious because of phreatic evaporation and the agricultural irrigation from wells, along with irrigation from surface inflow of Yellow River, quality of groundwater hydrochemistry during the dry season was ameliorative greatly. Consequently, it was very important to the agriculture sustainable production that well-canal combined irrigation patterns alleviated extremely alkaline trend of the groundwater hydrochemistry and played a positive role of root layer soil salinity leaching.

Preparation of Diphenylcarbazide-Directed Cd Se Quantum Dots and Selective Determination for Cr(Ⅵ)

Cd Se quantum dots（QDs） were synthesized using diphenylcarbazide（DL） to sequester QDs precursors（Cd^（2＋）） in situ. Fluorescence（FL） analysis showed the successive synthesis of QDs could be realized by capping with DL and the binding between DL and Cd^（2＋）. The average QDs particle size was about 5-20 nm by high-resolution transmission electron microscopy（HRTEM）. Fourier transform infrared（FT-IR） spectra showed that Cd Se QDs could be chemically bonded with DL. The formation of QDs-DL-Cr（Ⅵ） could lower the fluorescence intensity of QDs. In a certain concentration range, the fluorescence intensity and Cr（Ⅵ） concentration presented a linear relationship. As a result, this phenomenon could be used to determine the Cr（Ⅵ） concentration in the range of 0-24 ×10^（-6） mol· L^（-1）.

Effect of Deficit Irrigation on the Growth, Water Use Characteristics and Yield of Cotton in Arid Northwest China

Water shortage is a key constraint to sustainable agricultural production in Xinjiang, Northwest China. To enhance the use efficiency of valuable irrigation water resources, a 2-year experiment(2010–2011) was conducted to quantify the response of cotton(Gossypium hirsutum L.) growth and yield to different degrees of deficit irrigation(DI) regimes; to determine the effects of DI on the characteristics of water use for cotton, seasonal water use, available soil water in the root zone, soil water depletion, evapotranspiration(ET)-based water use efficiency and irrigation-based water use efficiency, and to determine the best DI regime for optimal water-saving and yield output. The plots were irrigated at 100%(100ET), 85%(85ET), 70%(70ET), 55%(55ET) and 45%(45ET) of the regional ET of cotton in northern Xinjiang. The effect of DI irrigation on water use characteristics was evaluated by analyzing available soil water and soil water depletion in the root zone along with water use efficiencies of cotton. The study showed that the growth, water use characteristics and yield of cotton varied with irrigation regime. Seasonal ET and seed cotton yield were linearly correlated with irrigation amount. The second-order polynomial equation best approximated water-yield relationship of cotton in the study area.Cotton yield response factor was 0.65, suggesting limited water conditions were suitable for cotton cultivation. Economic evaluation of DI treatments confirmed that the yield loss was less than 10% under 70 ET and 85 ET, which was acceptable for greater sustainability.The results suggested that proper DI schemes were necessary for sustainable cotton production in the region. While irrigation at 85 ET was safe for high cotton yield, irrigation at 70 ET was a viable alternative under limited irrigation water availability.

Response of spatial structure of cotton root to soil-wetting patterns under mulched drip irrigation

The matching relationship between the spatial structure of cotton cluster root systems and soil-wetting patterns under mulched drip irrigation forms the theoretical basis for the technical design of mulched drip irrigation.A 2-year field experiment was conducted,in which different soil-wetting patterns were produced by setting different emitter discharge rates.The envelopes of cotton cluster root length densities were derived using the topological methodology and used to examine the effects of different soil-wetting patterns on the spatial structure of root systems and water uptake capacity within row spaces.The results showed that the root systems in rows of cotton grown under narrower and deeper soil-wetting patterns exhibited a single-peak distribution,while those under wider and shallower soil-wetting patterns exhibited a two-peak distribution.Furthermore,cotton rows grown near mulch edges experienced lower moisture stress,and wider and shallower soil-wetting patterns contributed to greater root growth rates in the vertical direction and resulted in more even potential water uptake capacities.The findings of this study revealed that wider and shallower soil-wetting patterns were more desirable for mulched drip irrigation of cotton and should be considered in the technical design of drip irrigation systems.

Effects of Bacillus subtilis and Saccharomyces cerevisiae inoculation on soil bacterial community and rice yield under combined irrigation with reclaimed and fresh water

In order to save fresh water and reduce soil salt accumulation,reclaimed water-fresh water combined irrigation,i.e,irrigation with reclaimed water for 50 d and then with fresh water till harvest,was used in rice planting.Bacillus subtilis and Saccharomyces cerevisiae were inoculated into the soil at the end of reclaimed water irrigation.The inoculation weight per pot of these microorganisms was as follows:0 g and 0 g(J0),5 g and 0 g(J1),3.75 g and 1.25 g(J2),2.5 g and 2.5 g(J3),1.25 g and 3.75 g(J4),and 0 g and 5 g(J5),respectively.Treatment using reclaimed water in the whole stage was used as the control(CK).The plant height,tiller,physical and chemical properties of the soil,and soil bacterial diversity were measured.It was found that the plant height of rice was increased significantly by J1-J5 treatments.The dry weight of rice root,stem,and panicle and the 1000-grain weight increased significantly,while the leaf dry weight decreased.Microorganism inoculation significantly increased the nutrient absorption capacity of the crops.J1,J2,and J4 treatments significantly increased the amount of nitrate-nitrogen,ammonium nitrogen,available phosphorus,and available potassium,while J3,J4,and J5 treatments increased the soil organic matter,and microbial inoculation significantly decreased the EC of soil.J4 treatment induced the largest reduction in EC,and microorganisms treatments increased soil pH.Bacterial function prediction based on the KEGG(Kyoto Encyclopedia of Genes and Genomes)pathway indicated that soil metabolic function was not significantly disturbed by the treatments.Organic matter and pH are the two main factors affecting the structure of the bacterial community in soil.3.75 g of B.subtilis and 1.25 g of S.cerevisiae per pot is the best inoculation ratio.

Estimation of crop water requirement based on principal component analysis and geographically weighted regression

In this study the principal component analysis (PCA) and geographically weighted regression (GWR) are combined to estimate the spatial distribution of water requirement of the winter wheat in North China while the effect of the macroand micro-topographic as well as the meteorological factors on the crop water requirement is taking into account. The spatial distribution characteristic of the water requirement of the winter wheat in North China and its formation are analyzed based on the spatial variation of the main affecting factors and the regression coefficients. The findings reveal that the collinearity can be effectively removed when PCA is applied to process all of the affecting factors. The regression coefficients of GWR displayed a strong variability in space, which can better explain the spatial differences of the effect of the affecting factors on the crop water requirement. The evaluation index of the proposed method in this study is more efficient than the widely used Kriging method. Besides, it could clearly show the effect of those affecting factors in different spatial locations on the crop water requirement and provide more detailed information on the region where those factors suddenly change. To sum up, it is of great reference significance for the estimation of the regional crop water requirement.