Observation on Therapeutic Effect of Lotus Needle Cupping Therapy for Removing Blood Stasis in Zhuang Medicine on PHN and Its Influ-ence on Inflammatory Mediators

[Objectives]To observe the clinical effect of the lotus needle cupping therapy for removing blood stasis in Zhuang medicine in treating PHNand its influence on inflammatory factors.[Methods]96 patients with PHN were randomly divided into three groups:lotus nee-dle cupping therapy group,TCM surrounding acupuncture group and gabapentin group.Venous blood and acupoint blood were collected at 0,21 and 42 d of treatment,and the expression levels of 5-HT,SP and CGRPinflammatory mediators were detected before and after treatment.The changes of VAS scores before,during and after treatment and the clinical efficacy were observed.[Results]The total effective rate of the lotus needle cupping group was 87.50%,which was better than that of the TCM acupuncture group(81.25%)and the gabapentin group(62.50%);after treatment,the VAS scores and the expression of inflammatory mediators in the three groups of patients were lower than those before treatment,and the decrease was more significant in the treatment group(P<0.05).[Conclusions]The lotus needle cupping therapy for removing blood stasis in Zhuang medicine is effective in treating PHN,and its mechanism is to reduce the release of inflammatory media-tors,reduce hyperalgesia,relieve pain and improve the quality of life of patients.

Summary and clinical application of Zhuang medicine medicated thread moxibustion

Zhuang medicine medicated thread moxibustion is a kind of external therapy with ethnic medicine characteristics that spreads in Guangxi Zhuang region.It has a wide range of clinical application and significant curative effect,and can be used to treat diseases such as internal medicine,surgery,dermatology,gynecology,pediatrics,and five sense organs.Through reviewing and combing the literature on the Zhuang medicine medicated thread moxibustion in recent years,this paper summarizes and prospects its clinical application,with a view to further explaining the basic principles and application rules of Zhuang medicine medicated thread moxibustion and digging its clinical value.

A Preliminary Study on Effects of Zhuang Medicine Clerodendrum japonicum(Thunb.)Sweet on Acute Tonsillitis

[Objectives]To study the effect of Zhuang medicine Clerodendrum japonicum(Thunb.)Sweet on acute tonsillitis.[Methods]The antibacterial,antipyretic,anti-inflammatory and analgesic effects of the extract of Zhuang medicine C.japonicum(Thunb.)Sweet were observed.[Results]Zhuang medicine C.japonicum(Thunb.)Sweet showed a good inhibitory effect onα-hemolytic streptococcus,β-hemolytic streptococcs,Staphylococcus citreus,Staphylococcus epidermidis,Staphylococcus aureus,Pseudomonas aeruginosa,Proteus and Escherichia coli;it showed a good inhibitory effect on the infection of Staphylococcus aureus andβ-hemolytic streptococcs in vivo;it could significantly reduce the elevated body temperature of rabbits injected with the triple vaccine and significantly increase the pain threshold of thermally stimulated mice.[Conclusions]Zhuang Medicine C.japonicum(Thunb.)Sweet has positive significance in the treatment of acute tonsillitis instead of antibiotics.

Quality Control of Zhuang Medicine Xiaoyan Zhiyang Lotion

[Objectives]To establish the TLC identification methods of Llicis Rotundae Cortex and Mahoniae Caulis and the determination method of syringin content in Zhuang Medicine Xiaoyan Zhiyang Lotion.[Methods]Thin layer chromatography(TLC)was used to qualitatively identify Llicis Rotundae Cortex and Mahoniae Caulis in Zhuang Medicine Xiaoyan Zhiyang Lotion.The quantitative analysis of syringin in Zhuang Medicine Xiaoyan Zhiyang Lotion was carried out by high-performance liquid chromatography(HPLC).Chromatographic conditions are as follows:Luna 5μm C_(18)((2)100 A(250 mm×4.6 mm,5μm)column was adopted;acetonitrile(10%)-water(90%)was as the mobile phase,and the flow rate was 1.0 mL/min;the detection wavelength was 265 nm;the sample size was 5μL;the column temperature was 30℃.[Results]Syringin had a good linear relationship with its peak area in the range of 0.190-2.856μg(R^(2)=0.9995).The RSD values of precision,stability and repeatability experiments were all lower than 2.5%,and the average sampling recovery rate(n=6)was 100.37%(RSD=-0.88%).[Conclusions]The method had the advantages of simple operation,strong specificity,good repeatability and stability,and can be used for the quality control of Zhuang Medicine Xiaoyan Zhiyang Lotion.

TLC Identification and Extraction Process of Rubiasin-1-methyl Ether from Yao Medicine Chuanlianzhu

[Objectives]To establish a thin-layer chromatography(TLC)method for the determination of rubiadin-1-methyl ether in Yao Medicine Chuanlianzhu(Damnacanthus giganteus).[Methods]A silica gel G thin-layer plate was adopted for TLC.Petroleum ether(60-90℃)-chloroform-methanol-water(7:15:3:1)was used as the developing solvent and inspected under ultraviolet lamp(365 nm).The content was determined by Inertsil ODS-3 C 18 column(4.60 mm×250 mm,5μm),mobile phase:acetonitrile-0.2%phosphoric acid gradient elution,detection wavelength 277 nm,flow rate 1.0 mL/min,column temperature 30℃,injection volume 10μL.[Results]The spots of 10 Chuanlianzhu samples from different origins showed the same color at the same position as the control,and the spots were clear and specific.The injection volume of rubiadin-1-methyl ether showed a good linear relationship in the range of 2.90-145μg(R=0.9996).The average recovery rate of rubiadin-1-methyl ether in the low,medium and high dose groups of Yao Medicine Chuanlianzhu was 98.72%,and RSD=1.78%.[Conclusions]This method can effectively identify Yao Medicine Chuanlianzhu medicinal materials and accurately determine the content of rubiadin-1-methyl ether in the medicinal materials.It provides a scientific basis for the development and utilization of Yao Medicine Chuanlianzhu medicinal resources.

Anti-inflammatory Mechanism of Yao Medicine Laggerae Alatae Herba

[Objectives]To study the anti-inflammatory effect of Laggerae Alatae Herba extract and its mechanism.[Methods]Inflammation models of xylene-induced ear edema in mice,acetic acid-induced increased permeability of abdominal capillaries in mice,and carrageenan-induced paw edema in mice were established;xylene-induced ear swelling model in bilateral adrenalectomized mice was established.The levels of MDA,NO and SOD in inflammatory tissues of paw were measured.[Results]Compared with the model group,the high and medium dose groups of Laggerae Alatae Herba extract had significant inhibitory effect on xylene-induced ear edema in mice,except for the low dose group(P>0.05);Laggerae Alatae Herba extract inhibited the increase of celiac capillary permeability induced by acetic acid and paw edema induced by carrageenan in mice.Compared with the model group,in the mice model with bilateral adrenal glands removed,the high and medium dose groups of Laggerae Alatae Herba extract could significantly inhibit the xylene induced ear swelling of the mice.The high and medium dose groups of Laggerae Alatae Herba extract could significantly decrease the levels of MDA and NO,and significantly increase the level of SOD in the paw tissue.[Conclusions]The Laggerae Alatae Herba extracts have anti-inflammatory activity,and the anti-inflammatory effect of the extracts does not depend on the hypothalamic-pituitary-adrenal axis(HPAA)system.In addition,the anti-inflammatory mechanism of Laggerae Alatae Herba extract is related to the decrease of MDA and NO and the increase of SOD.

Preliminary Study on Quality Analysis of Guangxi Zhuang Medicine Lysimachia foenum-graecum Hance

[Objectives]To establish a quality analysis method for Guangxi Zhuang medicine Lysimachia foenum-graecum Hance.[Methods]The moisture,total ash and alcohol-soluble extracts of L.foenum-graecum medicinal materials were determined in accordance with the inspection method of the Chinese Pharmacopoeia(2015).[Results]The 10 batches of L.foenum-graecum medicinal materials from different origins and different collection times in Guangxi all had the same plant morphology and medicinal properties.The moisture content was 5.69%-10.51%,the total ash content was 5.50%-8.61%,the acid-insoluble ash content was 0.42%-0.78%,and the extract was 15.28%-20.75%.[Conclusions]This study is expected to provide a scientific basis for the development and utilization of L.foenum-graecum medicinal materials and the establishment of its quality standards.

Advances in research on prevention and treatment of malignant tumors by Zhuang medicine

Zhuang medicine is the wisdom of the Zhuang people's longterm struggle against disease.It forms part of the traditional medicine of the motherland.In the prevention and treatment of cancer,it has formed the idea of"harmony between man and nature","three gas","three roads"and"two roads"."For the basic theory and the pathogenesis of virtual poison,establish the system theory of"regulating Qi,detoxifying and supplementing deficiency"as the basic rule.In recent years,with the increasing incidence of malignant tumors and mortality in the world,there is a need for multi-channel treatments,and the unique advantages of Zhuang medicine with anti-tumor have been re-emphasized.This article will provide an overview of Zhuang Medical's understanding of tumors,the pathogenesis of Zhuang medicine,the rule of treatment and the clinical and basic research of Zhuang medicine in cancer prevention and treatment,and provide theoretical support for the clinical practice and experimental research of Zhuang medicine in the treatment of tumor.

Effect of Different Processing Methods on Content ofβ-Asarone in the Zhuang Medicine Rhizoma Acori Tatarinowii

[Objectives]This paper aims to establish a method to simultaneously determine the content ofβ-asarone in Rhizoma Acori Tatarinowii dried by three different methods.[Methods]Reversed-phase high-performance liquid chromatography was used,and the chromatographic conditions were as follows:column,Thermo SCIENTIFIC Hypersil GOLD Dim.(mm);mobile phase,methanol-0.1%phosphoric acid(63∶37);column temperature,30℃;flow rate,1mL/min;detection wavelength,257 nm;sample size,10μL.[Results]The linear range of the injection volume ofβ-asarone was 49.28-246.40μg/mL(R=0.9993);the limit of quantification was 0.85 ng and the detection limit was 0.34 ng;the RSD values of precision,stability and reproducibility tests were all less than 3%;and the sample recovery rate was 98.53%-98.97%(RSD<3.00).The results show that the content ofβ-asarone was highest in shade-dried Rhizoma Acori Tatarinowii.The order ofβ-asarone content was as follows:Rhizoma Acori Tatarinowii dried in shade>Rhizoma Acori Tatarinowii dried at 55℃>Rhizoma Acori Tatarinowii dried at 60℃.[Conclusions]This method is sensitive,reliable,and reproducible.It can be used to simultaneously determine the content ofβ-asarone in Rhizoma Acori Tatarinowii dried by three different methods.

Epidemiological and Clinical Characteristics of Non-neonatal Tetanus Patients in Guangxi,China:An 11-year Retrospective Study(2011–2021)

Tetanus is the only non-communicable disease among vaccine-preventable infectious diseases.It is caused by an infection with Clostridium tetanus bacterium and is characterized by continuous tonic contraction and paroxysmal spasms of skeletal muscles throughout the body[1].

Intervention effect of Zhuangfang Rugan Huaxian Granules on CCl_(4)compound factor‑induced liver fibrosis rat model and its effect on the expression ofα‑SMA and E‑Cadherin

Objective:To study the intervention effect of Zhuangfang Soft Liver and Fibrous Granules on CCl_(4)compound factor-induced liver fibrosis rat model and its effect onα-SMA and E-Cadherin protein expression.Methods:Forty-eight male Wistar rats were randomly divided into a blank group,a pathological model group and a group of 16 rats in each group.The rats in the pathological model group and the pellet group were injected subcutaneously with 40%carbon tetrachloride(CCl_(4))oil combined with peanut oil to establish an animal model of rat liver fibrosis,and the pellets were administered by gavage three times a week for 8 weeks from the 5th week.The expression of oxidative stress oxidase dismutase(SOD)and malondialdehyde(MDA)was detected by enzyme-linked immunoassay(ELISA),and the expression ofα-smooth muscle actin(α-SMA)and E-calmodulin(E-Cadherin)was detected by RT-PCR andWestern Blot.expression.Results:Compared with the blank group,the serum ALT and AST levels of the rats in the pathological model group were significantly increased,and the serum ALT and AST levels of the rats in the soft liver and fibrous pellet group were significantly decreased(P<0.001).In the pathological model group,the lobular structure of liver tissue was mutilated and numerous liver fibrous tissues were abnormally proliferated,and the collagen fibers formed by the proliferation were confluent into dense fibrous intervals,thus turning into pseudolobular structure,and a large number of fatty degenerated hepatocytes of different shapes and sizes appeared inside the pseudolobular structure,and the diffuse infiltration of inflammatory cells was significant.Compared with the pathological model group,the degree of destruction of hepatic lobular structure was significantly reduced in the LBP group,and there were different degrees of proliferation of collagen fibrous tissue concentrated in the area from the central vein to the confluent area,and the fibrous septum was relatively loose,with a small amount of inflammatory cell infiltration,and the degree of infiltration was reduced compared with the pathological model group.Compared with the pathological model group,the SOD level was significantly higher in the group of liver softener and fibrous pellets compared with the pathological model group and the blank group(P<0.001),the MDA level was significantly lower in the group of liver softener and fibrous pellets compared with the pathological model group(P<0.001),the expression level ofα-SMA was significantly lower in the group of liver softener and fibrous pellets(P<0.001),and the expression level of E-Cadherin was significantly higher in the group of liver softener and fibrous pellets(P<0.001).Conclusion:Soft Liver Fibro Pellets can effectively inhibit liver fibrous tissue proliferation and reduce the serum ALT and AST levels in rats with CCl_(4)combined with peanut oil compound solution induced liver fibrosis,and its anti-liver fibrosis mechanism may be through protecting liver function,reducing the level of oxidative stress,down-regulating the expression ofα-SMA protein,up-regulating the expression of E-cadherin protein,and inhibiting epithelial-mesenchymal transition epithelial mesenchymal transformation(EMT)and thus exerting therapeutic effects on CCl_(4)compound-induced hepatic fibrosis in rats.

Research Progress in Anti-tumor Effects of Traditional Chinese Medicine and Its Extracts

Traditional Chinese medicine and its extracts have received widespread attention due to their significant anti-tumor effects and low adverse reactions.Many traditional Chinese medicine and its extracts have been applied in clinical tumor treatment.In this paper,the research progress in anti-tumor effects of plant-based traditional Chinese medicine and its extracts,animal-based traditional Chinese medicine and its extracts,and traditional Chinese medicine compound is reviewed.

Preliminary Study on Quality Analysis of Yao Medicine Pileostegia tomentella Hand.-Mazz.

[Objectives]To establish a method for quality analysis of Medicine Pileostegia tomentella Hand.-Mazz.[Methods]According to the testing method of Chinese Pharmacopoeia(2020 Edition),the water content,total ash and alcohol-soluble extract of P.tomentella Hand.-Mazz.were determined.[Results]10 batches of P.tomentella Hand.-Mazz.from different habitats and in different collection time in Guangxi all had the same plant morphology and medicinal properties.The experimental results showed that the water content was 7.92%-10.35%,the total ash content was 5.53%-6.62%,the acid-insoluble ash content was 0.59%-0.76%,and the extract content was 11.63%-16.12%.[Conclusions]The identification method in this study can be used for the quality analysis of P.tomentella Hand.-Mazz.,and the experimental results can provide a scientific basis for the development and utilization of P.tomentella Hand.-Mazz.and the establishment of its quality standard.

Anti-inflammatory Effect of Polysaccharide from Embelia parviflora Wall.on Rheumatoid Arthritis in Rats

[Objectives]To investigate the anti-inflammatory effect of Embelia parviflora Wall.polysaccharide on rheumatoid arthritis(RA)in rats.[Methods]RA rat model was induced by type II collagen.After successful modeling,the rats were divided into model group,positive group,low,medium and high dose of E.parviflora Wall.polysaccharide groups,and normal control group.Body mass,toe volume and arthritis index were measured,and thymus index and spleen index were calculated.The levels of interleukin-1β,interleukin-6 and tumor necrosis factor-αin serum and synovial tissue of ankle joint were detected by ELISA.[Results]Compared with the normal control group,the pathological changes such as synovial hyperplasia and unclear layer were observed in the model group,the body mass was decreased(P<0.05),the toe volume,arthritis index,thymus and spleen index were increased(P<0.05),and the levels of IL-1β,IL-6 and TNF-αin serum and ankle synovial tissue were increased(P<0.05).Compared with the model group,the histopathological changes in synovium of ankle joint in the positive group and the medium and high dose groups of E.parviflora Wall.polysaccharide were significantly improved,and the body mass was increased(P<0.05).The toe volume,arthritis index,thymus index and spleen index were decreased(P<0.05).The levels of IL-Iβ,IL-6 and TNF-αin serum and synovial tissue of ankle joint were increased(P<0.05),while there was no significant difference between the low dose group of E.parviflora Wall.polysaccharide and the model group(P>0.05).[Conclusions]E.parviflora Wall.polysaccharide can reduce the body's inflammatory response and improve RA,which may be related to the inhibition of the activation of inflammatory cytokines IL-1β,IL-6 and TNF-α.

Pharmacodynamic Experiment of Dachengqi Decoction and Separated Decoction on Incomplete Intestinal Obstruction in Rats

[Objectives]To investigate the effect and mechanism of Dachengqi Decoction and separated decoction on incomplete intestinal obstruction in rats.[Methods]80 healthy SD rats were selected to establish incomplete intestinal obstruction model by silk ligation.The dosage was 20 mL/kg for 3 d,and the damage index of ileocecal mucosa was analyzed;the morphology of ileocecal mucosa was observed by HE staining;the serum levels of IL-1α,IL-1β,IL-6,IL-18,Ach,NO,ET,IL-1,TNF-αand ultra-micro Na+-K+-ATPase were detected by ELISA.[Results]Compared with the model group,the mucosal damage index of Dachengqi Decoction and each separated decoction group decreased significantly(P<0.05);compared with the normal group and sham operation group,the serum level of IL-1,IL-6,TNF-αand other factors in the model group increased significantly(P<0.05);compared with the model group,the serum IL-1,IL-6 and TNF-αsecretion levels of rats in Dachengqi Decoction group and separated decoction group decreased(P<0.01).[Conclusions]Dachengqi Decoction and each separated decoction can effectively improve intestinal tissue pathological damage in the incomplete intestinal obstruction model rats,and reduce the inflammatory reaction in the rat body.

Content Determination of Flavonoids in Radix Millettiae Speciosae from Different Areas of Guangxi

[Objectives] This study aimed to investigate the quality of Radix Millettiae Speciosae at different planting sites in Guangxi. [Methods] The content of flavonoids in Radix Millettiae Speciosae was determined by HPLC. The chromatographic conditions were as follows: column, ZORBAX SB-C_(18); mobile phase, acetonitrile-0.1% glacial acetic acid(42∶58); detection wavelength, 250(formononetin) and 310(maackiain) nm; flow rate, 1.0 mL/min; and column temperature, 30℃. [Results] Under the chromatographic conditions above, formononetin and maackiain could be completely separated from impurities. The standard curve had a good linear relationship(R^2>0.999 9). The precision and stability met analysis requirements. The average recovery rates were 97.53% and 98.54%, respectively, and the RSD values were 2.03% and 1.88%, respectively, indicating that the established method has good reproducibility, durability and accuracy. The content of formononetin was highest in the Radix Millettiae Speciosae from Yongning District, Nanning City, and the content of maackiain was highest in the Radix Millettiae Speciosae from Xichang Town, Hepu County. [Conclusions] The HPLC method established in this study is simple, accurate and stable. It can be used as a quality control method for Radix Millettiae Speciosae. The contents of flavonoids in Radix Millettiae Speciosae from Yongning District, Nanning City and Xichang Town, Hepu County were higher than those from other planting bases.

Analysis and Study on Quality Control of Yao Medicine Damnacanthus macrophyllus Based on Methods in Chinese Pharmacopoeia

[Objectives]To establish a quality analysis method for Yao medicine Damnacanthus macrophyllus Sieb.ex Miq.var.giganteus(Makino)Koidz..[D.indicus Gaertn.f.var.giganteus Makino].[Methods]The moisture,total ash and alcohol extracts of D.macrophyllus medicinal materials were determined according to the experimental methods in the Chinese Pharmacopoeia(2020).[Results]All the 10 batches of D.macrophyllus medicinal materials from different collection times and different regions in Guangxi had the same plant morphological characteristics.The experimental results indicate that the moisture was 9.81%-11.52%,the total ash content was 7.37%-9.53%,the acid-insoluble ash content was 1.10%-1.97%,and the extract was 8.25%-10.22%.[Conclusions]This study provides a scientific basis for establishing a quality standard for Yao medicine D.macrophyllus medicinal materials,and it is expected to better utilize and develop D.macrophyllus.

Determination of Kaempferol Content in 10 Batches of Different Production Areas of Yao Medicine Lysimachia foenum-graecum Hance

[Objectives]To determine the kaempferol content in Yao medicine Lysimachia foenum-graecum Hance.[Methods]The high performance liquid chromatography(HPLC)method was used.The chromatographic column:Inertsil ODS-3 C18 column(4.60 mm×250 mm,5μm);mobile phase:acetonitrile-0.1%glacial acetic acid;flow rate:1.0 mL/min;column temperature:30℃;detection wavelength:360 nm;sample injection volume:10μL.[Results]The kaempferol content in Yao medicine L.foenum-graecum was determined,Y=20652X-151098(R=0.9997)shows a good linear relationship within the linear range of 9.80-156.8μg/mL.[Conclusions]The high performance liquid chromatography(HPLC)method was established for determining the kaempferol content in Yao medicine L.foenum-graecum.This method is expected to provide a scientific basis for the formulation of quality control methods for L.foenum-graecum.

Potential Preventive and Therapeutic Effects of Traditional Chinese Medicine Compound Prescription and Traditional Chinese Medicine Products on COVID-19

This paper mainly analyzes the traditional Chinese medicine compound prescription and traditional Chinese medicine products with remarkable epidemic prevention effect since the outbreak of COVID-19,and discusses their potential prevention and treatment effects,so as to provide a basis for the further development and application of traditional Chinese medicine compound prescription and traditional Chinese medicine products against COVID-19.

Extraction Technology and Content Determination of Total Flavonoids from Traditional Chinese Medicine Yipichou

[Objectives]The research aimed to optimize extraction technology of total flavonoids from traditional Chinese medicine Yipichou,and establish methods of extraction and content determination of its medicinal materials.[Methods]Using reflux extraction method,total flavonoids of Yipichou were extracted.Via single-factor test,influence of extraction method,extraction solvent concentration,extraction volume and extraction time on content of total flavonoids from Yipichou was inspected.Using orthogonal test,extraction conditions were optimized,and optimal extraction technology of total flavonoids from Yipichou was obtained.[Results]The optimal extraction process of total flavonoids from Yipichou was as below:70%of methanol,1∶40 of solid-liquid ratio,and 1.0 h of reflux extraction time.[Conclusions]In the test,total flavonoids were extracted from Yipichou,with better repeatability,and stable and feasible method,which could provide certain scientific basis for studying quality standard of this medicinal material.