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Congenital anomalies of coronary artery misdiagnosed as coronary dilatations in Kawasaki disease:A clinical predicament
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作者 Rakesh Kumar Pilania Pallavi L Nadig +7 位作者 Suprit Basu Reva Tyagi Abarna Thangaraj Ridhima Aggarwal Munish Arora Arun Sharma Surjit Singh Manphool Singhal 《World Journal of Clinical Pediatrics》 2025年第1期93-100,共8页
BACKGROUND 2D-echocardiography(2DE)has been the primary imaging modality in children with Kawasaki disease(KD)to assess coronary arteries.AIM To report the presence and implications of incidental congenital coronary a... BACKGROUND 2D-echocardiography(2DE)has been the primary imaging modality in children with Kawasaki disease(KD)to assess coronary arteries.AIM To report the presence and implications of incidental congenital coronary artery anomalies that had been misinterpreted as coronary artery abnormalities(CAAs)on 2DE.METHODS Records of children diagnosed with KD,who underwent computed tomography coronary angiography(CTCA)at our center between 2013-2023 were reviewed.We identified 3 children with congenital coronary artery anomalies in this cohort on CTCA.Findings of CTCA and 2DE were compared in these 3 children.RESULTS Of the 241 patients with KD who underwent CTCA,3(1.24%)had congenital coronary artery anomalies on CTCA detected incidentally.In all 3 patients,baseline 2DE had identified CAAs.CTCA was then performed for detailed evaluation as per our unit protocol.One(11-year-boy)amongst the 3 patients had complete KD,while the other two(3.3-year-boy;4-month-girl)had incomplete KD.CTCA revealed separate origins of left anterior descending artery and left circumflex from left sinus[misinterpreted as dilated left main coronary artery(LCA)on 2DE],single coronary artery(interpreted as dilated LCA on 2DE)and dilated right coronary artery on 2DE in case of anomalous origin of LCA from the main pulmonary artery.The latter one was subsequently operated upon.CONCLUSION CTCA is essential for detailed assessment of coronary arteries in children with KD especially in cases where there is suspicion of congenital coronary artery anomalies.Relying solely on 2DE may not be sufficient in such cases,and findings from CTCA can significantly impact therapeutic decision-making. 展开更多
关键词 Coronary artery abnormalities Congenital coronary artery anomalies Computed tomography coronary angiography Kawasaki disease 2-dimensional echocardiography
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Exploring the genetic control of sweat gland characteristics in beef cattle for enhanced heat tolerance
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作者 Aakilah S.Hernandez Gabriel A.Zayas +5 位作者 Eduardo E.Rodriguez Kaitlyn M.Sarlo Davila Fahad Rafiq Andrea N.Nunez Cristiane Goncalves Titto Raluca G.Mateescu 《Journal of Animal Science and Biotechnology》 SCIE CAS CSCD 2024年第5期1820-1831,共12页
Background Thermal stress in subtropical regions is a major limiting factor in beef cattle production systems with around$369 million being lost annually due to reduced performance.Heat stress causes numerous physiolo... Background Thermal stress in subtropical regions is a major limiting factor in beef cattle production systems with around$369 million being lost annually due to reduced performance.Heat stress causes numerous physiological and behavioral disturbances including reduced feed intake and decreased production levels.Cattle utilize various physiological mechanisms such as sweating to regulate internal heat.Variation in these traits can help identify genetic variants that control sweat gland properties and subsequently allow for genetic selection of cattle with greater thermotolerance.Methods This study used 2,401 Brangus cattle from two commercial ranches in Florida.Precise phenotypes that contribute to an animal's ability to manage heat stress were calculated from skin biopsies and included sweat gland area,sweat gland depth,and sweat gland length.All animals were genotyped with the Bovine GGP F250K,and BLUPF90 software was used to estimate genetic parameters and for Genome Wide Association Study.Results Sweat gland phenotypes heritability ranged from 0.17 to 0.42 indicating a moderate amount of the phenotypic variation is due to genetics,allowing producers the ability to select for favorable sweat gland properties.A weighted single-step GWAS using sliding 10 kb windows identified multiple quantitative trait loci(QTLs)explaining a significant amount of genetic variation.QTLs located on BTA7 and BTA12 explained over 1.0%of genetic variance and overlap the ADGRV1 and CCDC168 genes,respectively.The variants identified in this study are implicated in processes related to immune function and cellular proliferation which could be relevant to heat management.Breed of Origin Alleles(BOA)were predicted using local ancestry in admixed populations(LAMP-LD),allowing for identification of markers'origin from either Brahman or Angus ancestry.A BOA GWAS was performed to identify regions inherited from particular ancestral breeds that might have a significant impact on sweat gland phenotypes.Conclusions The results of the BOA GWAS indicate that both Brahman and Angus alleles contribute positively to sweat gland traits,as evidenced by favorable marker effects observed from both genetic backgrounds.Understanding and utilizing genetic traits that confer better heat tolerance is a proactive approach to managing the impacts of climate change on livestock farming. 展开更多
关键词 GWAS HERITABILITY SKIN THERMOTOLERANCE
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抗CD1d单克隆抗体的制备及其识别结构域的鉴定 被引量:2
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作者 刘莹 李德敏 +2 位作者 徐小宁 刘雪松 金伯泉 《细胞与分子免疫学杂志》 CAS CSCD 北大核心 2007年第10期959-960,共2页
目的:制备抗人CD1d单克隆抗体(mAb)并研究其结合特性。方法:应用淋巴细胞杂交瘤技术制备鼠源性抗人CD1d mAb。用免疫荧光染色和流式细胞术鉴定其识别位点。结果:获得1株分泌抗CD1d mAb的杂交瘤细胞株。mAb的腹水ELISA效价达10-6,为IgG1... 目的:制备抗人CD1d单克隆抗体(mAb)并研究其结合特性。方法:应用淋巴细胞杂交瘤技术制备鼠源性抗人CD1d mAb。用免疫荧光染色和流式细胞术鉴定其识别位点。结果:获得1株分泌抗CD1d mAb的杂交瘤细胞株。mAb的腹水ELISA效价达10-6,为IgG1亚类,可识别293T细胞转染的CD1d分子的α1结构域。结论:获得1株可特异性识别CD1dα1结构域的mAb,为进一步研究CD1d的结构及功能提供了手段。 展开更多
关键词 CD1D 单克隆抗体 结合位点
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CD4^+CD25^+调节T细胞在免疫耐受及自身免疫病中的作用 被引量:3
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作者 张烜 陈万军 《基础医学与临床》 CSCD 北大核心 2005年第9期785-788,共4页
越来越多的研究表明调节T细胞是外周免疫耐受的关键。CD4+CD25+调节T细胞在免疫耐受、自身免疫病、移植、肿瘤等方面均有着极其重要的作用,并需要TGF-β参与。在很多自身免疫性疾病如SLE、炎症性肠病中,CD4+CD25+调节T细胞的数量和/或... 越来越多的研究表明调节T细胞是外周免疫耐受的关键。CD4+CD25+调节T细胞在免疫耐受、自身免疫病、移植、肿瘤等方面均有着极其重要的作用,并需要TGF-β参与。在很多自身免疫性疾病如SLE、炎症性肠病中,CD4+CD25+调节T细胞的数量和/或功能存在显著异常,且多与疾病活动正相关。通过对自身免疫病外周血T细胞亚群进行修饰再自体回输可能是治疗这类疾病新的突破。 展开更多
关键词 调节T细胞 TGF-Β 自身免疫病
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Acute kidney injury spectrum in patients with chronic liver disease:Where do we stand? 被引量:16
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作者 Wiwat Chancharoenthana Asada Leelahavanichkul 《World Journal of Gastroenterology》 SCIE CAS 2019年第28期3684-3703,共20页
Acute kidney injury (AKI) is a common complication of liver cirrhosis and is of the utmost clinical and prognostic relevance. Patients with cirrhosis, especially decompensated cirrhosis, are more prone to develop AKI ... Acute kidney injury (AKI) is a common complication of liver cirrhosis and is of the utmost clinical and prognostic relevance. Patients with cirrhosis, especially decompensated cirrhosis, are more prone to develop AKI than those without cirrhosis. The hepatorenal syndrome type of AKI (HRS–AKI), a spectrum of disorders in prerenal chronic liver disease, and acute tubular necrosis (ATN) are the two most common causes of AKI in patients with chronic liver disease and cirrhosis. Differentiating these conditions is essential due to the differences in treatment. Prerenal AKI, a more benign disorder, responds well to plasma volume expansion, while ATN requires more specific renal support and is associated with substantial mortality. HRS–AKI is a facet of these two conditions, which are characterized by a dysregulation of the immune response. Recently, there has been progress in better defining this clinical entity, and studies have begun to address optimal care. The present review synopsizes the current diagnostic criteria, pathophysiology, and treatment modalities of HRS–AKI and as well as AKI in other chronic liver diseases (non-HRS–AKI) so that early recognition of HRS–AKI and the appropriate management can be established. 展开更多
关键词 ACUTE kidney injury Acute-on-chronic LIVER failure Chronic LIVER disease Hepatorenal syndrome PLASMA perfusion and bilirubin ADSORPTION and double PLASMA MOLECULAR absorption SYSTEM Fractionated PLASMA separation and ADSORPTION MOLECULAR adsorbent recycling SYSTEM Single-pass albumin dialysis
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Following a TRAIL: Update on a ligand and its five receptors 被引量:27
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作者 Fiona C. KIMBERLEY Gavin R. SCREATON 《Cell Research》 SCIE CAS CSCD 2004年第5期359-372,共14页
Identification of tumour necrosis factor apoptosis inducing ligand (TRAIL), a TNF family ligand, sparked a torrent of research, following an initial observation that it could kill tumour cells, but spare normal cells.... Identification of tumour necrosis factor apoptosis inducing ligand (TRAIL), a TNF family ligand, sparked a torrent of research, following an initial observation that it could kill tumour cells, but spare normal cells. Almost a decade after its discovery, and with five known receptors, the true physiological role of TRAIL is still debated and its anti-tumorigenic properties limited by potential toxicity. This review takes a comprehensive look at the story of this enigmatic ligand, addressing its remaining potential as a therapeutic and providing an overview of the TRAIL receptors themselves. 展开更多
关键词 TRAIL apoptosis cancer.
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Helicobacter pylori and skin autoimmune diseases 被引量:10
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作者 Eli Magen Jorge-Shmuel Delgado 《World Journal of Gastroenterology》 SCIE CAS 2014年第6期1510-1516,共7页
Autoimmune skin diseases are characterized by dysregulation of the immune system resulting in a loss of tolerance to skin self-antigen(s). The prolonged interaction between the bacterium and host immune mechanisms mak... Autoimmune skin diseases are characterized by dysregulation of the immune system resulting in a loss of tolerance to skin self-antigen(s). The prolonged interaction between the bacterium and host immune mechanisms makes Helicobacter pylori(H. pylori) a plausible infectious agent for triggering autoimmunity. Epidemiological and experimental data now point to a strong relation of H. pylori infection on the development of many extragastric diseases, including several allergic and autoimmune diseases. H. pylori antigens activate cross-reactive T cells and induce autoantibodies production. Microbial heat shock proteins(HSP) play an important role of in the pathogenesis of autoimmune diseases because of the high level of sequence homology with human HSP. Eradication of H. pylori infection has been shown to be effective in some patients with chronic autoimmune urticaria, psoriasis, alopecia areata and Schoenlein-Henoch purpura. There is conflicting and controversial data regarding the association of H. pylori infection with Beh et's disease, scleroderma and autoimmune bullous diseases. No data are available evaluating the association of H. pylori infection with other skin autoimmune diseases, such as vitiligo, cutaneous lupus erythematosus and dermatomyositis. The epidemiological and experimental evidence for a possible role of H. pylori infection in skin autoimmune diseases are the subject of this review. 展开更多
关键词 AUTOIMMUNE SKIN HELICOBACTER PYLORI INFECTION
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Diagnosis of gluten related disorders: Celiac disease, wheat allergy and non-celiac gluten sensitivity 被引量:9
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作者 Luca Elli Federica Branchi +5 位作者 Carolina Tomba Danilo Villalta Lorenzo Norsa Francesca Ferretti Leda Roncoroni Maria Teresa Bardella 《World Journal of Gastroenterology》 SCIE CAS 2015年第23期7110-7119,共10页
Cereal crops and cereal consumption have had a vital role in Mankind's history. In the recent years gluten ingestion has been linked with a range of clinical disorders. Gluten-related disorders have gradually emer... Cereal crops and cereal consumption have had a vital role in Mankind's history. In the recent years gluten ingestion has been linked with a range of clinical disorders. Gluten-related disorders have gradually emerged as an epidemiologically relevant phenomenon with an estimated global prevalence around 5%. Celiac disease, wheat allergy and non-celiac gluten sensitivity represent different gluten-related disorders. Similar clinical manifestations can be observed in these disorders, yet there are peculiar pathogenetic pathways involved in their development. Celiac disease and wheat allergy have been extensively studied, while non-celiac gluten sensitivity is a relatively novel clinical entity, believed to be closely related to other gastrointestinal functional syndromes. The diagnosis of celiac disease and wheat allergy is based on a combination of findings from the patient's clinical history and specific tests, including serology and duodenal biopsies in case of celiac disease, or laboratory and functional assays for wheat allergy. On the other hand, non-celiac gluten sensitivity is still mainly a diagnosis of exclusion, in the absence of clear-cut diagnostic criteria. A multimodal pragmatic approach combining findings from the clinical history, symptoms, serological and histological tests is required in order to reach an accurate diagnosis. A thorough knowledge of the differences and overlap in clinical presentation among gluten-related disorders, and between them and other gastrointestinal disorders, will help clinicians in the process of differential diagnosis. 展开更多
关键词 CELIAC disease GLUTEN sensitivity Wheatsensitivity ALLERGY GLUTEN-FREE DIET
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Interleukin-1β gene polymorphism associated with hepatocellular carcinoma in hepatitis B virus infection 被引量:14
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作者 Nattiya Hirankarn Ingorn Kimkonq +2 位作者 Pittaya Kummee Pisit Tanqkijyanich Yong Poovorawan 《World Journal of Gastroenterology》 SCIE CAS CSCD 2006年第5期776-779,共4页
AIM: To examine the effect of interleukin-l-beta (IL-113) promoter region C-511T and IL-1 receptor antagonist (IL-1RN) polymorphism among the patients with chronic hepatitis B virus (HBV) infection (HCC and no... AIM: To examine the effect of interleukin-l-beta (IL-113) promoter region C-511T and IL-1 receptor antagonist (IL-1RN) polymorphism among the patients with chronic hepatitis B virus (HBV) infection (HCC and non-HCC). METHODS: Genomic DNA from 136 Thai patients with chronic HBV infection (HCC =46 and non-HCC= 90) and 152 healthy individuals was genotyped for IL-113 gene polymorphism (-511) using polymerase chain reaction with sequence specific primers (PCR-SSP). The variable number of tandem repeats (VNTR) of IL-1RN gene was assessed by a PCR-based assay. The association between these genes and status of the disease was evaluated by X^2 test. RESULTS: IL-1B-511 genotype c/c was found to be significantly different in patients with HCC when compared with healthy individuals (P = 0.036, OR = 2.29, 95%CI = 1.05-4.97) and patients without HCC (P=0.036, OR= 2.52, 95%CI=1.05-6.04). Analysis of allele frequencies of IL-1B-511 showed that IL-1B-511 C allele was also significantly increased in patients with HCC, compared to that in healthy control (P=0.033, OR= 1.72, 95%CI=1.04-2.84). However, no significant association in IL-1RN gene was found between the two groups. CONCLUSION: IL-1B-511C allele, which may be associated with high IL-1B production in the liver, is a genetic marker for the development of HCC in chronic hepatitis B patients in Thai population. 展开更多
关键词 Interleukin-1 beta gene POLYMORPHISM Hepatocellular carcinoma Hepatitis B
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Autologous CD34^+ and CD133^+ stem cells transplantation in patients with end stage liver disease 被引量:16
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作者 Hosny Salama Abdel-Rahman N Zekri +6 位作者 Abeer A Bahnassy Eman Medhat Hanan A Halim Ola S Ahmed Ghada Mohamed Sheren A Al Alim Ghada M Sherif 《World Journal of Gastroenterology》 SCIE CAS CSCD 2010年第42期5297-5305,共9页
AIM:To assess the utility of an autologous CD34 + and CD133 + stem cells infusion as a possible therapeutic modality in patients with end-stage liver diseases.METHODS:One hundred and forty patients with endstage liver... AIM:To assess the utility of an autologous CD34 + and CD133 + stem cells infusion as a possible therapeutic modality in patients with end-stage liver diseases.METHODS:One hundred and forty patients with endstage liver diseases were randomized into two groups.Group 1,comprising 90 patients,received granulocyte colony stimulating factor for five days followed by autologous CD34 + and CD133 + stem cell infusion in the portal vein.Group 2,comprising 50 patients,received regular liver treatment only and served as a control group.RESULTS:Near normalization of liver enzymes and improvement in synthetic function were observed in 54.5% of the group 1 patients;13.6% of the patients showed stable states in the infused group.None of the patients in the control group showed improvement.No adverse effects were noted.CONCLUSION:Our data showed that a CD34 + and CD133 + stem cells infusion can be used as supportive treatment for end-stage liver disease with satisfactory tolerability. 展开更多
关键词 CD34 CD133 Stem cell Liver Hepatitis C virus
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Clinical significance of donor-specific human leukocyte antigen antibodies in liver transplantation 被引量:6
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作者 Antonio Cuadrado David San Segundo +2 位作者 Marcos López-Hoyos Javier Crespo Emilio Fábrega 《World Journal of Gastroenterology》 SCIE CAS 2015年第39期11016-11026,共11页
Antibody-mediated rejection(AMR) caused by donorspecific anti-human leukocyte antigen antibodies(DSA) is widely accepted to be a risk factor for decreased graft survival after kidney transplantation. This entity also ... Antibody-mediated rejection(AMR) caused by donorspecific anti-human leukocyte antigen antibodies(DSA) is widely accepted to be a risk factor for decreased graft survival after kidney transplantation. This entity also plays a pathogenic role in other solid organ transplants as it appears to be an increasingly common cause of heart graft dysfunction and an emerging issue in lung transplantation. In contrast, the liver appears relatively resistant to DSA-mediated injury. This "immune-tolerance" liver property has been sustained by a low rate of liver graft loss in patients with preformed DSA and by the intrinsic liver characteristics that favor the absorption and elimination of DSA; however, alloantibody-mediated adverse consequences are increasingly being recognized, and several cases of acute AMR after ABO-compatible liver transplant(LT) have been reported. Furthermore, the availability of new solid-phase assays, allowing the detection of low titers of DSA and the refinement of objective diagnostic criteria for AMR in solid organ transplants and particularly in LT, have improved the recognition and management of this entity. A cost-effective strategy of DSA monitoring, avoidance of class Ⅱ human leukocyte antigen mismatching, judicious immunosuppression attached to a higher level of clinical suspicion of AMR, particularly in cases unresponsive to conventional antirejection therapy, can allow a rational approach to this threat. 展开更多
关键词 Donor-specific anti-human LEUKOCYTE ANTIGEN antibo
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Mismatch repair genes (hMLH1, hPMS1, hPMS2, GTBP/hMSH6,HMSH2) in the pathogenesis of hepatocellular carcinoma 被引量:10
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作者 Abdel-Rahman N. Zekri Gelane M. Sabry +3 位作者 Abeer A. Bahnassy Kamal A. Shalaby Sabrin A.Abdel-Wahabh Serag Zakaria 《World Journal of Gastroenterology》 SCIE CAS CSCD 2005年第20期3020-3026,共7页
AIM: DMA mismatch repair (MMR) is an important mechanism for maintaining fidelity of genomic DNA. Abnormalities in one or more MMR genes are implicated in the development of many cancers. We investigated the role of e... AIM: DMA mismatch repair (MMR) is an important mechanism for maintaining fidelity of genomic DNA. Abnormalities in one or more MMR genes are implicated in the development of many cancers. We investigated the role of expression of MMR genes (hMLH1, hPMS1, hPMS2, GTBP/hMSH6, hMSH2) in hepatocellular carcinogenesis. METHODS: We evaluated the expression level of MMR genes in 33 hepatocellular carcinoma (HCC) cases using the multiplex reverse transcription (RT) PCR assays, as well as in 16 cases of normal adjacent hepatic tissues. β-actin gene was used as an internal control and calibrator for quantification of gene expression. RESULTS: Out of the 33 studied cases, 25 were HCV positive and 30 (90.9%) showed reduced expression in one or more of the studied MMR genes. Reduced expression was found in hMSH2(71.9%), hMLH1 (53.3%), GTBP(51.1%), hPMS2 (33.3%) and hPMS1 (6%). A significant correlation was found between reduced expression of hPMS2(P= 0.0069) and GTBP(P= 0.0034), hPMS2 and non-cirrhosis (P= 0.0197), hMLH1 and high grade. On the other hand, 57.1%, 50%, 20%, 18.8%, and 6% of the normal tissues distant to tumors showed reduced expression of hMSH2, hMLH1, GTBP, hPMS2, and hPMS1 respectively. Multivariate analysis revealed a significant correlation between the expression level of hMSH2(P= 0.008), hMLH1 (P= 0.001) and GTBP (P= 0.032) and HCC, between hPMS2, GTBP and HCV-associated HCC (P<0.001, 0.002). CONCLUSION: Reduced expression of MMR genes seems to play an important role in HCV-associated HCC. hPMS2 is likely involved at an early stage of hepatocarcinogenesis since it was detected in normal adjacent tissues. Reduced expression of hPMS2 provides a growth advantage and stimulates proliferation which encourages malignant transformation in non-cirrhotic HCV-infected patients via acquisition of more genetic damages. 展开更多
关键词 Hepatocellular carcinoma Mismatch repair Normal distant hepatic tissue
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Hepatitis B virus reactivation in hepatitis B virus surface antigen negative patients receiving immunosuppression: A hidden threat 被引量:6
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作者 Kalliopi Zachou Alexandros Sarantopoulos +6 位作者 Nikolaos K Gatselis Themistoklis Vassiliadis Stella Gabeta Aggelos Stefos Asterios Saitis Panagiota Boura George N Dalekos 《World Journal of Hepatology》 CAS 2013年第7期387-392,共6页
AIM: To present the characteristics and the course of a series of anti- hepatitis B virus core antibody (HBc) antibody positive patients, who experienced hepatitis B virus (HBV) reactivation after immunosuppression. M... AIM: To present the characteristics and the course of a series of anti- hepatitis B virus core antibody (HBc) antibody positive patients, who experienced hepatitis B virus (HBV) reactivation after immunosuppression. METHODS: We retrospectively evaluated in our tertiary centers the medical records of hepatitis B virus surface antigen (HBsAg) negative patients who suffered from HBV reactivation after chemotherapy or immunosuppression during a 3-year period (2009-2011). Accordingly, the clinical, laboratory and virological characteristics of 10 anti-HBc (+) anti-HBs (-)/HBsAg (-) and 4 anti-HBc (+)/antiHBs (+)/HBsAg (-) patients, who developed HBV reactivation after the initiation of chemotherapy or immunosuppressive treatment were analyzed. Quantitative determination of HBV DNA during reactivation was performed in all cases by a quantitative real time polymerase chain reaction kit (COBAS Taqman HBV Test; cut-off of detection: 6 IU/mL). RESULTS: Twelve out of 14 patients were males; median age 74.5 years. In 71.4% of them the primary diagnosis was hematologic malignancy; 78.6% had received rituximab (R) as part of the immunosuppressive regimen. The median time from last chemotherapy schedule till HBV reactivation for 10 out of 11 patients who received R was 3 (range 2-17) mo. Three patients (21.4%) deteriorated, manifesting ascites and hepatic encephalopathy and 2 (14.3%) of them died due to liver failure. CONCLUSION: HBsAg-negative anti-HBc antibody positive patients can develop HBV reactivation even 2 years after stopping immunosuppression, whereas prompt antiviral treatment on diagnosis of reactivation can be lifesaving. 展开更多
关键词 IMMUNOSUPPRESSION HEPATITIS B ANTIHEPATITIS B VIRUS core antibody POSITIVITY OCCULT HEPATITIS B VIRUS infection Rituximab
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Role of relevant immune-modulators and cytokines in hepatocellular carcinoma and premalignant hepatic lesions 被引量:6
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作者 Abdel-Rahman N Zekri Somaya El Deeb +8 位作者 Abeer A Bahnassy Abeer M Badr Mona S Abdellateif Gamal Esmat Hosny Salama Marwa Mohanad Ahmed Esam El-dien Shimaa Rabah Assmaa Abd Elkader 《World Journal of Gastroenterology》 SCIE CAS 2018年第11期1228-1238,共11页
AIM To assess the levels of different immune modulators in patients with hepatocellular carcinoma(HCC),in relation to other hepatic diseases.METHODS Eighty-eight patients were included in the current study and represe... AIM To assess the levels of different immune modulators in patients with hepatocellular carcinoma(HCC),in relation to other hepatic diseases.METHODS Eighty-eight patients were included in the current study and represented patients with HCC(20),liver cirrhosis(28) and chronic hepatitis(CH;25),and normal controls(NC;15).Peripheral blood was isolated for immunophenotyping of active myeloid dendritic cells(m DCs;CD1 c and CD40),mature inactive myeloid cells(CD1 c and HLA),active plasmacytoid cells(p DCs;CD303 and CD40),mature inactive p DCs(CD30 and HLA),active natural killer(NK) cells(CD56 and CD161),active NK cells(CD56 and CD314) and inactive NK cells(CD56 and CD158) was done by flow cytometry.Serum levels of interleukin(IL)-2,IL-10,IL-12,IL-1β,interferon(IFN)-α,IFN-γ and tumor necrosis factor(TNF)-αR2 were assessed by ELISA.RESULTS Active m DCs(CD1 C+/CD40+) and inactive m DCs(CD1 c+/HLA+) were significantly decreased in HCC patients in relation to NC(P < 0.001).CD40+ expression on active p DCs was decreased in HCC patients(P < 0.001),and its level was not significantly changed among other groups.Inactive p DCs(CD303+/HLA+),inactive NKs(CD56+/CD158+) and active NKs(CD56+/CD161+) were not statistically changed among the four groups studied;however,the latter was increased in CH(P < 0.05).NKG2 D was statistically decreased in HCC,CH and cirrhosis(P < 0.001),and it was not expressed in 63%(12/20) of HCC patients.There was significant decrease of IL-2,IFN-α and IFN-γ(P < 0.001),and a significant increase in IL-10,IL-1β,and TNF-αR2(P <0.01,P < 0.001 and P < 0.001;respectively) in HCC patients.There was inverted correlation between IL-12 and IL-1β in HCC(r =-0.565,P < 0.01),with a strong correlation between p DCs(CD303+/CD40+) and NKs(CD56+/CD161+;r = 0.512,P < 0.05) as well as inactive m DCs(CD1 c+/HLA+) and inactive NK cells(CD56+/CD158+;r = 0.945,P < 0.001).CONCLUSION NKG2 D,CD40,IL-2 and IL-10 are important modulators in the development and progression of HCC. 展开更多
关键词 Hepatocellular carcinoma Hepatitis C virus NKG2D CD40 INTERLEUKIN-2 INTERLEUKIN-10 myeloid dendritic CELLS PLASMACYTOID CELLS natural killer cell CYTOKINES
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Cytokine profile in Egyptian hepatitis C virus genotype-4 in relation to liver disease progression 被引量:4
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作者 Abdel-Rahman N Zekri Mohammed S El-Din Ashour +3 位作者 Ahmed Hassan Hanaa M Alam El-Din Amal MR El-Shehaby Maha A Abu-Shady 《World Journal of Gastroenterology》 SCIE CAS CSCD 2005年第42期6624-6630,共7页
AIM: To observe the imbalance between T helper cell Th1 and Th2 cytokines in several chronic hepatitis disease at different stages of disease progression. METHODS: We measured the cytokine levels of Thl (IL-2 and I... AIM: To observe the imbalance between T helper cell Th1 and Th2 cytokines in several chronic hepatitis disease at different stages of disease progression. METHODS: We measured the cytokine levels of Thl (IL-2 and IL-2R), Th2 (IL-10) and the pro-inflammatory cytokines (IL-6 and IL-6R and TNF and TNF-RI and II) by the ELISA technique in the sera of 33 hepatocellular carcinoma (HCC) patients and 20 chronic liver disease (CLD) patients. In addition, 20 asymptomatic hepatitis C virus carriers and 20 healthy subjects negative for hepatitis C virus(HCV) markers served as controls. RESULTS: Anti-HCV antibodies were found to be positive in 94% of HCC cases and 75% of CLD cases. On the other hand, HCV viremia was detected using RT- PCR in 67% of HCC cases and 65% of CLD cases. HBsAg was positive in 9% of HCC cases and 30% of CLD cases. Also bilharziaI-Ab was positive in 55% of HCC cases, 65% of CLD cases and in 70% of asymptomatic carriers (ASC). HCC patients had significantly higher values of IL- 2R, TNF-RII (P〈0.001), and TNF-RI (P〉0.05), but lower TNFα (P〈0.001) and IL-6 (P = 0.032) in comparison to ASC. But, in comparison to non-cancer controls, HCC patients had higher values of IL-2R, IL-6R, TNF-RI and TNF-RII, but lower TNF-α (P〈0.001). CLD patients had higher IL-2R, TNF-RI, and TNF-RII (P〈0.001) than ASC. But, in comparison to non-cancer controls, CLD patients had higher values of IL-2R, TNF-RI and TNF-RII, but lower TNF-α (P〈0.001). IL-10 was higher (though not significantly) in HCC and CLD patients than in symptomatic carriers and non-cancer controls. CONCLUSION: Liver disease progression from CLD to HCC due to HCV genotype-4 infection is associated with an imbalance between Th1 and Th2 cytokines. IL-2R, TNF-RI, and TNF-RII could be used as potential markers. 展开更多
关键词 CYTOKINE HCV Genotype-4 Liver disease
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Early detection of hepatocellular carcinoma co-occurring with hepatitis C virus infection: A mathematical model 被引量:2
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作者 Abdel-Rahman Nabawy Zekri Amira Salah El-Din Youssef +6 位作者 Yasser Mabrouk Bakr Reham Mohamed Gabr Ola Sayed Ahmed Mostafa Hamed Elberry Ahmed Mahmoud Mayla Mohamed Abouelhoda Abeer A Bahnassy 《World Journal of Gastroenterology》 SCIE CAS 2016年第16期4168-4182,共15页
AIM: To develop a mathematical model for the early detection of hepatocellular carcinoma (HCC) with a panel of serum proteins in combination with &#x003b1;-fetoprotein (AFP).METHODS: Serum levels of interleukin (I... AIM: To develop a mathematical model for the early detection of hepatocellular carcinoma (HCC) with a panel of serum proteins in combination with &#x003b1;-fetoprotein (AFP).METHODS: Serum levels of interleukin (IL)-8, soluble intercellular adhesion molecule-1 (sICAM-1), soluble tumor necrosis factor receptor II (sTNF-RII), proteasome, and &#x003b2;-catenin were measured in 479 subjects categorized into four groups: (1) HCC concurrent with hepatitis C virus (HCV) infection (n = 192); (2) HCV related liver cirrhosis (LC) (n = 96); (3) Chronic hepatitis C (CHC) (n = 96); and (4) Healthy controls (n = 95). The R package and different modules for binary and multi-class classifiers based on generalized linear models were used to model the data. Predictive power was used to evaluate the performance of the model. Receiver operating characteristic curve analysis over pairs of groups was used to identify the best cutoffs differentiating the different groups.RESULTS: We revealed mathematical models, based on a binary classifier, made up of a unique panel of serum proteins that improved the individual performance of AFP in discriminating HCC patients from patients with chronic liver disease either with or without cirrhosis. We discriminated the HCC group from the cirrhotic liver group using a mathematical model (-11.3 + 7.38 &#x000d7; Prot + 0.00108 &#x000d7; sICAM + 0.2574 &#x000d7; &#x003b2;-catenin + 0.01597 &#x000d7; AFP) with a cutoff of 0.6552, which achieved 98.8% specificity and 89.1% sensitivity. For the discrimination of the HCC group from the CHC group, we used a mathematical model [-10.40 + 1.416 &#x000d7; proteasome + 0.002024 &#x000d7; IL + 0.004096 &#x000d7; sICAM-1 + (4.251 &#x000d7; 10<sup>-4</sup>) &#x000d7; sTNF + 0.02567 &#x000d7; &#x003b2;-catenin + 0.02442 &#x000d7; AFP] with a cutoff 0.744 and achieved 96.8% specificity and 89.7% sensitivity. Additionally, we derived an algorithm, based on a binary classifier, for resolving the multi-class classification problem by using three successive mathematical model predictions of liver disease status.CONCLUSION: Our proposed mathematical model may be a useful method for the early detection of different statuses of liver disease co-occurring with HCV infection. 展开更多
关键词 Mathematical model Hepatocellular carcinoma α -FETOPROTEIN Soluble intercellular adhesion molecule-1 β -CATENIN INTERLEUKIN-8 Soluble tumor necrosis factor receptor II PROTEASOME
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Circulating tumor and cancer stem cells in hepatitis C virusassociated liver disease 被引量:9
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作者 Abeer A Bahnassy Abdel-Rahman N Zekri +6 位作者 Ahmed El-Bastawisy Amal Fawzy Marwa Shetta Nehal Hussein Dalia Omran Abdallah A S Ahmed Samir S El-Labbody 《World Journal of Gastroenterology》 SCIE CAS 2014年第48期18240-18248,共9页
AIM: To assess the role of circulating tumor cells (CTCs) and cancer stem cells (CSCs) in hepatitis C virus (HCV)-associated liver disease.
关键词 Cancer stem cells Circulating tumor cells Hepatitis C virus genotype-4 Hepatocellular carcinoma
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NKT cells in HIV-1 infection 被引量:2
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作者 Demin Li Xiao-Ning Xu 《Cell Research》 SCIE CAS CSCD 2008年第8期817-822,共6页
Natural killer T (NKT) cells are a unique T cell population that have important immunoregulatory functions and have been shown to be involved in host immunity against a range of microorganisms. It also emerges that ... Natural killer T (NKT) cells are a unique T cell population that have important immunoregulatory functions and have been shown to be involved in host immunity against a range of microorganisms. It also emerges that they might play a role in HIV-1 infection, and therefore be selectively depleted during the early stages of infection. Recent studies are reviewed regarding the dynamics of NKT depletion during HIV-1 infection and their recovery under highly active antiretroviral treatment (HAART). Possible mechanisms for these changes are proposed based on the recent developments in HIV pathogenesis. Further discussions are focused on HIV's disruption of NKT activation by downregulating CDld expression on antigen presentation cells (APC). HIV-1 protein Nefis found to play the major role by interrupting the intracellular trafficking of nascent and recycling CDld molecules. 展开更多
关键词 NKT cells HIV-1 CDId downregulation
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Serum proteins in chronic hepatitis B patients treated with peginterferon alfa-2b 被引量:4
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作者 Sunida Kuakarn Poorichaya SomParn +3 位作者 Pisit Tangkijvanich Varocha Mahachai Visith Thongboonkerd Nattiya Hirankarn 《World Journal of Gastroenterology》 SCIE CAS 2013年第31期5067-5075,共9页
AIM: To study the differential protein profile in serum of hepatitis B patients.METHODS: Serum samples were obtained from patients with chronic hepatitis B who were receiving peginterferon alfa-2b.The serum samples we... AIM: To study the differential protein profile in serum of hepatitis B patients.METHODS: Serum samples were obtained from patients with chronic hepatitis B who were receiving peginterferon alfa-2b.The serum samples were subjected to albumin depletion and analyzed by two-dimensional gel electrophoresis(2-DE).Differentially expressed protein spots were identified by electrospray ionizationquadrupole time-of-flight mass spectrometry.Alpha2-HS-glycoprotein,complement component C3c and CD5 antigen were further analyzed by an enzymelinked immunosorbent assay and immunonephelometry.RESULTS: Nineteen patients with HBeAg-positive chronic hepatitis B(CHB) were studied.These patients were followed for at least 1 year after treatment and were classified according to their treatment response: responders(n = 9) and non-responders(n = 10).2-DE and MS/MS analysis were performed to compare the serum proteins before initiating peginterferon alfa2b.From the quantitative analysis of the 2-D gel,7 proteins were detected between the two groups at different levels before treatment.Among these potential candidates,serum levels of alpha-2-HS-glycoprotein,complement component C3c and CD5 antigen-like precursor were further analyzed.In the validation phase,23 subjects,9 sustained responders and 14 nonresponders,were recruited.Interestingly,the levels of alpha-2-HS-glycoprotein and complement component C3c were elevated in the serum of the non-responders compared to the responders.CONCLUSION: Serum alpha-2-HS-glycoprotein and complement component C3c may be potential serum biomarkers in predicting the treatment response of peginterferon alfa-2b in patients with CHB prior to treatment. 展开更多
关键词 PROTEOMICS PEGINTERFERON alfa-2b CHRONIC HEPATITIS B Alpha-2-HS-glycoprotein SERUM
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Seroprevalence of celiac disease among healthy adolescents in Saudi Arabia 被引量:1
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作者 Abdulrahman M Aljebreen Majid A Almadi +1 位作者 Alwaleed Alhammad Faleh Z Al Faleh 《World Journal of Gastroenterology》 SCIE CAS 2013年第15期2374-2378,共5页
AIM: To identify the seroprevalence of celiac disease among healthy Saudi adolescents. METHODS: Between December 2007 and January 2008, healthy students from the 10th to 12th grades were randomly selected from three r... AIM: To identify the seroprevalence of celiac disease among healthy Saudi adolescents. METHODS: Between December 2007 and January 2008, healthy students from the 10th to 12th grades were randomly selected from three regions in Saudi Arabia. These regions included the following: (1) Aseer region, with a student population of 25512; (2) Madinah, with a student population of 23852; and (3) Al-Qaseem, with a student population of 16067. Demographic data were recorded, and a venous blood sample (5-10 mL) was taken from each student. The blood samples were tested for immunoglobulin A and immunoglobulin G endomysial antibodies (EMA) by indirect immunofluorescence.RESULTS: In total, 1167 students (614 males and 553 females) from these three regions were randomly selected. The majority of the study population was classified as lower middle class (82.7%). There were 26 (2.2%) students who had a positive anti-EMA test, including 17 females (3.1%) and 9 males (1.5%). Al-Qaseem region had the highest celiac disease prevalence among the three studied regions in Saudi Arabia (3.1%). The prevalence by region was as follows: Aseer 2.1% (10/479), Madinah 1.8% (8/436), and Al-Qaseem 3.2% (8/252). The prevalence in Madinah was significantly lower than the prevalence in Aseer and Al-Qaseem (P=0.02). CONCLUSION: Our data suggest celiac disease prevalence might be one of the highest in the world. Further studies are needed to determine the real prevalence. 展开更多
关键词 CELIAC disease SAUDI ARABIA Prevalence Antiendomysial ANTIBODY EPIDEMIOLOGY
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