期刊文献+
共找到38篇文章
< 1 2 >
每页显示 20 50 100
Discovering Cathodic Biocompatibility for Aqueous Zn–MnO_(2) Battery:An Integrating Biomass Carbon Strategy 被引量:1
1
作者 Wei Lv Zilei Shen +10 位作者 Xudong Li Jingwen Meng Weijie Yang Fang Ding Xing Ju Feng Ye Yiming Li Xuefeng Lyu Miaomiao Wang Yonglan Tian Chao Xu 《Nano-Micro Letters》 SCIE EI CAS CSCD 2024年第6期111-126,共16页
Developing high-performance aqueous Zn-ion batteries from sustainable biomass becomes increasingly vital for large-scale energy storage in the foreseeable future.Therefore,γ-MnO_(2) uniformly loaded on N-doped carbon... Developing high-performance aqueous Zn-ion batteries from sustainable biomass becomes increasingly vital for large-scale energy storage in the foreseeable future.Therefore,γ-MnO_(2) uniformly loaded on N-doped carbon derived from grapefruit peel is successfully fabricated in this work,and particularly the composite cathode with carbon carrier quality percentage of 20 wt%delivers the specific capacity of 391.2 mAh g^(−1)at 0.1 A g^(−1),outstanding cyclic stability of 92.17%after 3000 cycles at 5 A g^(−1),and remarkable energy density of 553.12 Wh kg^(−1) together with superior coulombic efficiency of~100%.Additionally,the cathodic biosafety is further explored specifically through in vitro cell toxicity experiments,which verifies its tremendous potential in the application of clinical medicine.Besides,Zinc ion energy storage mechanism of the cathode is mainly discussed from the aspects of Jahn–Teller effect and Mn domains distribution combined with theoretical analysis and experimental data.Thus,a novel perspective of the conversion from biomass waste to biocompatible Mn-based cathode is successfully developed. 展开更多
关键词 Aqueous Zn-ion batteries BIOCOMPATIBILITY Jahn-Teller effect Mn domains γ-MnO_(2)
下载PDF
Extending the spatiotemporal resolution of super-resolution microscopies using photomodulatable °uorescent proteins 被引量:1
2
作者 Mingshu Zhang Zhifei Fu Pingyong Xu 《Journal of Innovative Optical Health Sciences》 SCIE EI CAS 2016年第3期52-62,共11页
In the past two decades,various super-resolution(SR)microscopy techniques have been developed to break the di®raction limit using subdi®raction excitation to spatially modulate the°uorescence emission.P... In the past two decades,various super-resolution(SR)microscopy techniques have been developed to break the di®raction limit using subdi®raction excitation to spatially modulate the°uorescence emission.Photomodulatable°uorescent proteins(FPs)can be activated by light of speci¯c wavelengths to produce either stochastic or patterned subdi®raction excitation,resulting in improved optical resolution.In this review,we focus on the recently developed photomodulatable FPs or commonly used SR microscopies and discuss the concepts and strategies for optimizing and selecting the biochemical and photophysical properties of PMFPs to improve the spatiotemporal resolution of SR techniques,especially time-lapse live-cell SR techniques. 展开更多
关键词 SUPER-RESOLUTION PMFP mGeos mEos3 Skylan-S Skylan-NS
下载PDF
Phosphoglucose isomerase gene expression as a prognostic biomarker of gastric cancer 被引量:1
3
作者 Han-Chen Huang Xian-Zi Wen +3 位作者 Hua Xue Run-Sheng Chen Jia-Fu Ji Lei Xu 《Chinese Journal of Cancer Research》 SCIE CAS CSCD 2019年第5期771-784,共14页
Objective: Tumor heterogeneity renders identification of suitable biomarkers of gastric cancer(GC)challenging. Here, we aimed to identify prognostic genes of GC using computational analysis.Methods: We first used micr... Objective: Tumor heterogeneity renders identification of suitable biomarkers of gastric cancer(GC)challenging. Here, we aimed to identify prognostic genes of GC using computational analysis.Methods: We first used microarray technology to profile gene expression of GC and paired nontumor tissues from 198 patients. Based on these profiles and patients’ clinical information, we next identified prognostic genes using novel computational approaches. Phosphoglucose isomerase, also known as glucose-6-phosphate isomerase(GPI), which ranked first among 27 candidate genes, was further investigated by a new analytical tool namely enviro-geno-pheno-state(E-GPS) analysis. Suitability of GPI as a prognostic marker, and its relationship with physiological processes such as metabolism, epithelial-mesenchymal transition(EMT), as well as drug sensitivity were evaluated using both our own and independent public datasets.Results: We found that higher expression of GPI in GC correlated with prolonged survival of patients.Particularly, a combination of CDH2 and GPI expression effectively stratified the outcomes of patients with TNM stage Ⅱ/Ⅲ. Down-regulation of GPI in tumor tissues correlated well with depressed glucose metabolism and fatty acid synthesis, as well as enhanced fatty acid oxidation and creatine metabolism, indicating that GPI represents a suitable marker for increased probability of EMT in GC cells.Conclusions: Our findings strongly suggest that GPI acts as a novel biomarker candidate for GC prognosis,allowing greatly enhanced clinical management of GC patients. The potential metabolic rewiring correlated with GPI also provides new insights into studying the relationship between cancer metabolism and patient survival. 展开更多
关键词 GASTRIC cancer gene expression profile PROGNOSTIC BIOMARKER phosphoglucose ISOMERASE tumor metabolism
下载PDF
AlphaFold2 and its applications in the fields of biology and medicine 被引量:10
4
作者 Zhenyu Yang Xiaoxi Zeng +1 位作者 Yi Zhao Runsheng Chen 《Signal Transduction and Targeted Therapy》 SCIE CSCD 2023年第4期1630-1643,共14页
AlphaFold2(AF2)is an artificial intelligence(AI)system developed by DeepMind that can predict three-dimensional(3D)structures of proteins from amino acid sequences with atomic-level accuracy.Protein structure predicti... AlphaFold2(AF2)is an artificial intelligence(AI)system developed by DeepMind that can predict three-dimensional(3D)structures of proteins from amino acid sequences with atomic-level accuracy.Protein structure prediction is one of the most challenging problems in computational biology and chemistry,and has puzzled scientists for 50 years.The advent of AF2 presents an unprecedented progress in protein structure prediction and has attracted much attention.Subsequent release of structures of more than 200 million proteins predicted by AF2 further aroused great enthusiasm in the science community,especially in the fields of biology and medicine.AF2 is thought to have a significant impact on structural biology and research areas that need protein structure information,such as drug discovery,protein design,prediction of protein function,et al.Though the time is not long since AF2 was developed,there are already quite a few application studies of AF2 in the fields of biology and medicine,with many of them having preliminarily proved the potential of AF2.To better understand AF2 and promote its applications,we will in this article summarize the principle and system architecture of AF2 as well as the recipe of its success,and particularly focus on reviewing its applications in the fields of biology and medicine.Limitations of current AF2 prediction will also be discussed. 展开更多
关键词 system FIELDS FOLD
原文传递
RNA biology and therapeutics
5
作者 Puyue Wang Yuanchao Xue +1 位作者 Yijun Qi Runsheng Chen 《Fundamental Research》 CAS CSCD 2023年第5期655-656,共2页
Eukaryotic genomes undergo pervasive transcription,generating vast amounts of noncoding RNAs alongside protein-coding mRNAs[1].These noncoding RNAs,including small noncoding RNAs,long noncoding RNAs(lncRNAs),and circu... Eukaryotic genomes undergo pervasive transcription,generating vast amounts of noncoding RNAs alongside protein-coding mRNAs[1].These noncoding RNAs,including small noncoding RNAs,long noncoding RNAs(lncRNAs),and circular RNAs,have been shown to play critical roles in gene regulation,chromatin remodeling,assembly of membraneless organelles,and other essential biological processes.They function through a diverse range of mechanisms[2],[3],[4],[5].Dysregulation of noncoding RNAs contributes to human disease pathogenesis and affects plant development and stress response[6],[7],[8].Over the past decade,significant progress has been made in unraveling the functions of noncoding RNAs and elucidating the molecular mechanisms by which they operate.The involvement of noncoding RNAs in human disease pathogenesis and agronomic trait regulation has garnered increasing attention. 展开更多
关键词 INVOLVEMENT PATHOGENESIS REMODELING
原文传递
Circular RNA circZbtb20 maintains ILC3 homeostasis and function via Alkbh5-dependent m6A demethylation of Nr4a1 mRNA 被引量:4
6
作者 Benyu Liu Nian Liu +7 位作者 Xiaoxiao Zhu Liuliu Yang Buqing Ye Huimu Li Pingping Zhu Tiankun Lu Yong Tian Zusen Fan 《Cellular & Molecular Immunology》 SCIE CAS CSCD 2021年第6期1412-1424,共13页
Group 3 innate lymphoid cells(ILC3s)play critical roles in innate immunity and gut homeostasis.However,how ILC3 homeostasis is regulated remains elusive.Here,we identified a novel circular RNA,circZbtb20,that is highl... Group 3 innate lymphoid cells(ILC3s)play critical roles in innate immunity and gut homeostasis.However,how ILC3 homeostasis is regulated remains elusive.Here,we identified a novel circular RNA,circZbtb20,that is highly expressed in ILC3s and required for their maintenance and function.CircZbtb20 deletion causes reduced ILC3 numbers,increasing susceptibility to C.rodentium infection.Mechanistically,circZbtb20 enhances the interaction of Alkbh5 with Nr4a1 mRNA,leading to ablation of the m6A modification of Nr4a1 mRNA to promote its stability.Nr4a1 initiates Notch2 signaling activation,which contributes to the maintenance of ILC3 homeostasis.Deletion of Alkbh5 or Nr4a1 also impairs ILC3 homeostasis and increases susceptibilities to bacterial infection.Thus,our findings reveal an important role of circular RNA in the regulation of innate lymphoid cell homeostasis. 展开更多
关键词 circZbtb20 ILC3 Alkbh5 Nr4a1 HOMEOSTASIS
原文传递
Structural insights reveal the specific recognition of meiRNA by the Mei2 protein 被引量:1
7
作者 Siyuan Shen Yanze jian +6 位作者 Zhaokui Cai Fudong Li Mengqi Lv Yongrui Liu Jihui Wu Chuanhai Fu Yunyu Shi 《Journal of Molecular Cell Biology》 SCIE CAS CSCD 2022年第5期27-42,共16页
In the fission yeast Schizosaccharomyces pombe,Mei2,an RNA-binding protein essential for entry into meiosis,regulates meiosis initiation.Mei2 binds to a specific non-coding RNA species,meiRNA,and accumulates at the sm... In the fission yeast Schizosaccharomyces pombe,Mei2,an RNA-binding protein essential for entry into meiosis,regulates meiosis initiation.Mei2 binds to a specific non-coding RNA species,meiRNA,and accumulates at the sme2 gene locus,which encodes meiRNA.Previous research has shown that the Mei2 C-terminal RNA recognition motif(RRM3)physically interacts with the meiRNA 5'region in vitro and stimulates meiosis in vivo.However,the underlying mechanisms still remain elusive.We first employed an in vitro crosslinking and immunoprecipitation sequencing(CLIP-seq)assay and demonstrated a preference for U-rich motifs of meiRNA by Mei2 RRM3.We then solved the crystal structures of Mei2 RRM3 in the apo form and complex with an 8 mer RNA fragment,derived from meiRNA,as detected by in vitro CLIP-seq.These results provide structural insights into the Mei2 RRM3-meiRNA complex and reveal that Mei2 RRM3 binds specifically to the Uuc(U)sequence.Furthermore,a structure-based Mei2 mutation,Mei2F644A causes defective karyogamy,suggesting an essential role of the RNA-binding ability of Mei2 in regulating meiosis. 展开更多
关键词 Mei2 meiRNA MEIOSIS RNA-binding protein CRYSTALLOGRAPHY
原文传递
Anti-CRISPRs: The natural inhibitors for CRISPR-Cas systems 被引量:1
8
作者 Fei Zhang Guoxu Song Yong Tian 《Animal Models and Experimental Medicine》 CSCD 2019年第2期69-75,共7页
CRISPR (clustered regularly interspaced short palindromic repeats)‐Cas (CRISPR associated protein) systems serve as the adaptive immune system by which prokaryotes defend themselves against phages. It has also been d... CRISPR (clustered regularly interspaced short palindromic repeats)‐Cas (CRISPR associated protein) systems serve as the adaptive immune system by which prokaryotes defend themselves against phages. It has also been developed into a series of powerful gene‐editing tools. As the natural inhibitors of CRISPR‐Cas systems, anti‐CRISPRs (Acrs) can be used as the “off‐switch” for CRISPR‐Cas systems to limit the off‐target effects caused by Cas9. Since the discovery of CRISPR‐Cas systems, much research has focused on the identification, mechanisms and applications of Acrs. In light of the rapid development and scientific significance of this field, this review summarizes the history and research status of Acrs, and considers future applications. 展开更多
关键词 anti‐CRISPRs Cas9 CRISPR‐Cas system
下载PDF
RNA structure determination:From 2D to 3D 被引量:1
9
作者 Jie Deng Xianyang Fang +6 位作者 Lin Huang Shanshan Li Lilei Xu Keqiong Ye Jinsong Zhang Kaiming Zhang Qiangfeng Cliff Zhang 《Fundamental Research》 CAS CSCD 2023年第5期727-737,共11页
RNA molecules serve a wide range of functions that are closely linked to their structures.The basic structural units of RNA consist of single-and double-stranded regions.In order to carry out advanced functions such a... RNA molecules serve a wide range of functions that are closely linked to their structures.The basic structural units of RNA consist of single-and double-stranded regions.In order to carry out advanced functions such as catalysis and ligand binding,certain types of RNAs can adopt higher-order structures.The analysis of RNA structures has progressed alongside advancements in structural biology techniques,but it comes with its own set of challenges and corresponding solutions.In this review,we will discuss recent advances in RNA structure analysis techniques,including structural probing methods,X-ray crystallography,nuclear magnetic resonance,cryo-electron microscopy,and small-angle X-ray scattering.Often,a combination of multiple techniques is employed for the integrated analysis of RNA structures.We also survey important RNA structures that have been recently determined using various techniques. 展开更多
关键词 RNA structure RNA structure probing X-ray crystallography Nuclear magnetic resonance spectroscopy Cryo-electron microscopy Small angle X-ray scattering
原文传递
Mechanosensing via Immunereceptors
10
作者 Cheng Zhu Wei Chen +2 位作者 Jizhong Lou William Rittase Kaitao Li 《医用生物力学》 EI CAS CSCD 北大核心 2019年第A01期9-9,共1页
The immune response is orchestrated by a variety of immune cells,the function of which then is determined by the collective signals from different immunoreceptors.Recent studies have highlighted the presence of mechan... The immune response is orchestrated by a variety of immune cells,the function of which then is determined by the collective signals from different immunoreceptors.Recent studies have highlighted the presence of mechanical force on these receptor-ligand pairs and its important role in regulating antigen recognition/discrimination and function.In this perspective,we use the T cell receptor as an example to review the current understanding of the mechanosensing properties of immunoreceptors.We discuss the types of forces that immunoreceptors may encounter,the effects on ligand recognition,conformational changes and mechanosensing mechanisms,as well as the consequences in downstream signal transduction and function. 展开更多
关键词 MECHANOSENSING Immunereceptors
下载PDF
Identification and analysis of intermediate-size noncoding RNAs in the rhesus macaque fetal brain
11
作者 Liyuan Zhu Xiaochao Tan +11 位作者 Wei Liu Fengbiao Mao Chao Wu Junjie Zhou Xiao Liu Shuaiyao Lu Kaili Ma Bin Yin Jianjun Luo Jiangang Yuan Boqin Qiang Runsheng Chen 《Journal of Genetics and Genomics》 SCIE CAS CSCD 2017年第3期171-174,共4页
Although only about 2%of the human genome has proved to be protein-coding genes,recent advances in genome wide analysis have revealed that the majority of the genome is transcribed,mainly from noncoding segments that ... Although only about 2%of the human genome has proved to be protein-coding genes,recent advances in genome wide analysis have revealed that the majority of the genome is transcribed,mainly from noncoding segments that were once considered"junk sequences"or"dark matters"(Liu et al.,2011a;Zhang et al.,2014b). In addition to the well-characterized housekeeping non- coding RNAs (ncRNAs) (tRNA, rRNA, small nuclear RNA and small nucleolar RNAs) and some small regulatory ncRNAs (microRNAs and small interfering RNAs), the transcriptome of mammals could also pervasively have been transcribed long noncoding RNAs (lncRNAs, at least 200 nt) (Rinn and Chang, 2012; Xie et al., 2012). 展开更多
关键词 RNA Identification and analysis of intermediate-size noncoding RNAs in the rhesus macaque fetal brain
原文传递
ADAR1:a mast regulator of aging and immunity
12
作者 Yigan Zhang Jinyue Zhang Yuanchao Xue 《Signal Transduction and Targeted Therapy》 SCIE CSCD 2023年第2期323-324,共2页
Recently,Nature and Nature Cell Biology published five papers on the function and molecular mechanism of ADAR1(adenosine deaminases acting on RNA)in aging,cancer,and autoimmune diseases.1,2,3,4,5 Among them,four paper... Recently,Nature and Nature Cell Biology published five papers on the function and molecular mechanism of ADAR1(adenosine deaminases acting on RNA)in aging,cancer,and autoimmune diseases.1,2,3,4,5 Among them,four papers published in Nature revealed that ADAR1 regulates autoimmune disease and cancer immunotherapy through canonical adenosine-to-inosine(A-to-I)RNA editing. 展开更多
关键词 ADAR1 IMMUNITY AUTOIMMUNE
原文传递
秀丽隐杆线虫RNA结合蛋白复合物AMG-1/SLRP-1通过线粒体稳态维持实现调控生殖腺发育和精子发生
13
作者 王鹏 王秋实 +15 位作者 陈联万 曹铮 赵海莲 苏瑞宝 王宁 马肖静 单进 陈新艳 张琦 杜宝臣 袁志恒 赵艳梅 张晓荣 郭雪江 薛愿超 苗龙 《Science Bulletin》 SCIE EI CAS CSCD 2023年第13期1399-1412,M0004,共15页
RNA结合蛋白(mtRBP)介导的mRNA转录后调节对精子发生必不可少但却鲜有报道.在本文中,我们鉴定到一个在生殖腺中特异性表达的线粒体RNA结合蛋白AMG-1,它是秀丽隐杆线虫精子发生过程中必需的蛋白,同时与哺乳动物LRPPRC蛋白同源.amg-1突变... RNA结合蛋白(mtRBP)介导的mRNA转录后调节对精子发生必不可少但却鲜有报道.在本文中,我们鉴定到一个在生殖腺中特异性表达的线粒体RNA结合蛋白AMG-1,它是秀丽隐杆线虫精子发生过程中必需的蛋白,同时与哺乳动物LRPPRC蛋白同源.amg-1突变会阻碍生殖腺的发育,最终导致生殖细胞的线粒体形态和结构异常以及线粒体功能障碍.通过测序鉴定RNA结合蛋白的靶点发现,AMG-1更倾向于与mtDNA编码的参与线粒体核糖体组装的12S和16S核糖体RNA(rRNA)结合,12S rRNA对于维持生殖细胞线粒体蛋白稳态至关重要,而12S rRNA的表达却受AMG-1蛋白调节.此外,哺乳动物SLIRP在秀丽线虫中的同源蛋白SLRP-1蛋白与AMG-1在遗传上存在互作关系,它们可共同调节秀丽线虫的精子发生和育性.综上所述,这些发现揭示了mtRBP蛋白AMG-1在线粒体调控中的新机制,这可能为由线粒体功能障碍引发的男性不育治疗提供新的理论基础. 展开更多
关键词 SPERMATOGENESIS RNA-binding protein mt-rRNA MITOCHONDRIA Caenorhabditis elegans
原文传递
Exploring noncoding variants in genetic diseases:from detection to functional insights 被引量:1
14
作者 Ke Wu Fengxiao Bu +6 位作者 Yang Wu Gen Zhang Xin Wang Shunmin He Mo-Fang Liu Runsheng Chen Huijun Yuan 《Journal of Genetics and Genomics》 SCIE CAS CSCD 2024年第2期111-132,共22页
Previous studies on genetic diseases predominantly focused on protein-coding variations, overlooking the vast noncoding regions in the human genome. The development of high-throughput sequencing technologies and funct... Previous studies on genetic diseases predominantly focused on protein-coding variations, overlooking the vast noncoding regions in the human genome. The development of high-throughput sequencing technologies and functional genomics tools has enabled the systematic identification of functional noncoding variants. These variants can impact gene expression, regulation, and chromatin conformation, thereby contributing to disease pathogenesis. Understanding the mechanisms that underlie the impact of noncoding variants on genetic diseases is indispensable for the development of precisely targeted therapies and the implementation of personalized medicine strategies. The intricacies of noncoding regions introduce a multitude of challenges and research opportunities. In this review, we introduce a spectrum of noncoding variants involved in genetic diseases, along with research strategies and advanced technologies for their precise identification and in-depth understanding of the complexity of the noncoding genome. We will delve into the research challenges and propose potential solutions for unraveling the genetic basis of rare and complex diseases. 展开更多
关键词 Noncoding variation Genetic disease Research approach CHALLENGE
原文传递
Molecular characteristics and evolutionary analysis of a very virulent infectious bursal disease virus 被引量:6
15
作者 LI Zan QI XiaoLe +3 位作者 REN XianGang CUI Lei WANG XiaoMei ZHU Ping 《Science China(Life Sciences)》 SCIE CAS CSCD 2015年第8期731-738,共8页
Infectious bursal disease virus (IBDV) poses a significant threat to the poultry industry. Viral protein 2 (VP2), the major struc- tural protein of IBDV, has been subjected to frequent mutations that have imparted... Infectious bursal disease virus (IBDV) poses a significant threat to the poultry industry. Viral protein 2 (VP2), the major struc- tural protein of IBDV, has been subjected to frequent mutations that have imparted tremendous genetic diversity to the virus. To determine how amino acid mutations may affect the virulence of IBDV, we built a structural model of VP2 of a very virulent strain of IBDV identified in China, vvIBDV Gx, and performed a molecular dynamics simulation of the interaction between virulence sites. The study showed that the amino acid substitutions that distinguish vvlBDV from attenuated IBDV (H253Q and T284A) favor a hydrophobic and flexible conformation of β-barrel loops in VP2, which could promote interac- tions between the virus and potential IBDV-specific receptors. Population sequence analysis revealed that the IBDV strains prevalent in East Asia show a significant signal of positive selection at virulence sites 253 and 284. In addition, a signal of co-evolution between sites 253 and 284 was identified. These results suggest that changes in the virulence of IBDV may result from both the interaction and the co-evolution of multiple amino acid substitutions at virulence sites. 展开更多
关键词 infectious bursal disease virus very virulent strain VIRULENCE molecular characterization molecular evolution
原文传递
Noncoding RNA:from dark matter to bright star 被引量:13
16
作者 Yuanchao Xue Runsheng Chen +1 位作者 Lianghu Qu Xiaofeng Cao 《Science China(Life Sciences)》 SCIE CAS CSCD 2020年第4期463-468,共6页
The central dogma states that genes encoded in the DNA should be first transcribed into messenger RNA(mRNA)and then translated into functional proteins(Crick,1970).This dogma has been written in numerous textbooks and... The central dogma states that genes encoded in the DNA should be first transcribed into messenger RNA(mRNA)and then translated into functional proteins(Crick,1970).This dogma has been written in numerous textbooks and learned by myriad students.However,along with the completion of the human genome project in June 2000,an astonishing fact was revealed:only 1.5%of the human genome encodes for proteins(Lander et al.,2001;Venter et al.,2001).This fact raised three fundamental questions:(i)why does the human genome have so few protein-coding genes?(ii)how to explain the apparent differences between humans and other species using the limited coding genes?(iii)what are the roles of the noncoding regions in our genome? 展开更多
关键词 al. APPARENT raised
原文传递
Liquid-liquid phase separation in biology: mechanisms,physiological functions and human diseases 被引量:26
17
作者 Hong Zhang Xiong Ji +7 位作者 Pilong Li Cong Liu Jizhong Lou Zheng Wang Wenyu Wen Yue Xiao Mingjie Zhang Xueliang Zhu 《Science China(Life Sciences)》 SCIE CAS CSCD 2020年第7期953-985,共33页
Cells are compartmentalized by numerous membrane-enclosed organelles and membraneless compartments to ensure that a wide variety of cellular activities occur in a spatially and temporally controlled manner. The molecu... Cells are compartmentalized by numerous membrane-enclosed organelles and membraneless compartments to ensure that a wide variety of cellular activities occur in a spatially and temporally controlled manner. The molecular mechanisms underlying the dynamics of membrane-bound organelles, such as their fusion and fission, vesicle-mediated trafficking and membrane contactmediated inter-organelle interactions, have been extensively characterized. However, the molecular details of the assembly and functions of membraneless compartments remain elusive. Mounting evidence has emerged recently that a large number of membraneless compartments, collectively called biomacromolecular condensates, are assembled via liquid-liquid phase separation(LLPS). Phase-separated condensates participate in various biological activities, including higher-order chromatin organization,gene expression, triage of misfolded or unwanted proteins for autophagic degradation, assembly of signaling clusters and actin-and microtubule-based cytoskeletal networks, asymmetric segregations of cell fate determinants and formation of pre-and post-synaptic density signaling assemblies. Biomacromolecular condensates can transition into different material states such as gel-like structures and solid aggregates. The material properties of condensates are crucial for fulfilment of their distinct functions, such as biochemical reaction centers, signaling hubs and supporting architectures. Cells have evolved multiple mechanisms to ensure that biomacromolecular condensates are assembled and disassembled in a tightly controlled manner. Aberrant phase separation and transition are causatively associated with a variety of human diseases such as neurodegenerative diseases and cancers. This review summarizes recent major progress in elucidating the roles of LLPS in various biological pathways and diseases. 展开更多
关键词 phase separation phase transition TRANSCRIPTION asymmetric division postsynaptic density AUTOPHAGY
原文传递
TDP-43 regulates cancer-associated microRNAs 被引量:4
18
作者 Xiaowei Chen Zhen Fan +9 位作者 Warren McGee Mengmeng Chen Ruirui Kong Pushuai Wen Tengfei Xiao Xiaomin Chen Jianghong Liu Li Zhu Runsheng Chen Jane Y. Wu 《Protein & Cell》 SCIE CAS CSCD 2018年第10期848-866,共19页
regulation of miRNA genes contributes to pathogenesis of a wide range of human diseases, including cancer. The TAR DNA binding protein 43 (TDP- 43), a RNAJDNA binding protein associated with neu- rodegeneration, is ... regulation of miRNA genes contributes to pathogenesis of a wide range of human diseases, including cancer. The TAR DNA binding protein 43 (TDP- 43), a RNAJDNA binding protein associated with neu- rodegeneration, is involved in miRNA biogenesis. Here, we systematically examined miRNAs regulated by TDP- 43 using RNA-Seq coupled with an siRNA-mediated knockdown approach. TDP-43 knockdown affected the expression of a number of miRNAs. In addition, TDP-43 down-regulation led to alterations in the patterns of dif- ferent isoforms of miRNAs (isomiRs) and miRNA arm selection, suggesting a previously unknown role of TDP- 43 in miRNA processing. A number of TDP-43 associ- ated miRNAs, and their candidate target genes, are associated with human cancers. Our data reveal highly complex roles of TDP-43 in regulating different miRNAs and their target genes. Our results suggest that TDP-43 may promote migration of lung cancer cells by regulat- ing miR-423-3p. In contrast, TDP-43 increases miR-500a- 3p expression and binds to the mature miR-500a-3p sequence. Reduced expression of miR-500a-3p is associated with poor survival of lung cancer patients,suggesting that TDP-43 may have a suppressive role in cancer by regulating miR-500a-3p. Cancer-associated genes LIF and PAPPA are possible targets of miR-500a- 3p. Our work suggests that TDP-43-regulated miRNAs may play multifaceted roles in the pathogenesis of cancer. 展开更多
关键词 TDP-43 MIRNA CANCER MIGRATION PROGNOSIS
原文传递
Crystal structure of Cas1 in complex with branched DNA 被引量:2
19
作者 Jing Yang Jiazhi Li +5 位作者 Jiuyu Wang Gang Sheng Min Wang Hongtu Zhao Yanhua Yang Yanli Wang 《Science China(Life Sciences)》 SCIE CAS CSCD 2020年第4期516-528,共13页
Cas1 is a key component of the CRISPR adaptation complex,which captures and integrates foreign DNA into the CRISPR array,resulting in the generation of new spacers.We have determined crystal structures of Thermus ther... Cas1 is a key component of the CRISPR adaptation complex,which captures and integrates foreign DNA into the CRISPR array,resulting in the generation of new spacers.We have determined crystal structures of Thermus thermophilus Cas1 involved in new spacer acquisition both in complex with branched DNA and in the free state.Cas1 forms an asymmetric dimer without DNA.Conversely,two asymmetrical dimers bound to two branched DNAs result in the formation of a DNA-mediated tetramer,dimer of structurally asymmetrical dimers,in which the two subunits markedly present different conformations.In the DNA binding complex,the N-terminal domain adopts different orientations with respect to the C-terminal domain in the two monomers that form the dimer.Substrate binding triggers a conformational change in the loop 164–177 segment.This loop is also involved in the 3′fork arm and 5′fork arm strand recognition in monomer A and B,respectively.This study provides important insights into the molecular mechanism of new spacer adaptation. 展开更多
关键词 CRISPR CAS protein Cas1 ADAPTATION DNA integration structure PROTEIN-DNA COMPLEX
原文传递
SmProt: A Reliable Repository with Comprehensive Annotation of Small Proteins Identified from Ribosome Profiling 被引量:2
20
作者 Yanyan Li Honghong Zhou +10 位作者 Xiaomin Chen Yu Zheng Quan Kang Di Hao Lili Zhang Tingrui Song Huaxia Luo Yajing Hao Runsheng Chen Peng Zhang Shunmin He 《Genomics, Proteomics & Bioinformatics》 SCIE CAS CSCD 2021年第4期602-610,共9页
Small proteins specifically refer to proteins consisting of less than 100 amino acids translated from small open reading frames(s ORFs),which were usually missed in previous genome annotation.The significance of small... Small proteins specifically refer to proteins consisting of less than 100 amino acids translated from small open reading frames(s ORFs),which were usually missed in previous genome annotation.The significance of small proteins has been revealed in current years,along with the discovery of their diverse functions.However,systematic annotation of small proteins is still insufficient.Sm Prot was specially developed to provide valuable information on small proteins for scientific community.Here we present the update of Sm Prot,which emphasizes reliability of translated s ORFs,genetic variants in translated s ORFs,disease-specific s ORF translation events or sequences,and remarkably increased data volume.More components such as non-ATG translation initiation,function,and new sources are also included.Sm Prot incorporated638,958 unique small proteins curated from 3,165,229 primary records,which were computationally predicted from 419 ribosome profiling(Ribo-seq)datasets or collected from literature and other sources from 370 cell lines or tissues in 8 species(Homo sapiens,Mus musculus,Rattus norvegicus,Drosophila melanogaster,Danio rerio,Saccharomyces cerevisiae,Caenorhabditis elegans,and Escherichia coli).In addition,small protein families identified from human microbiomes were also collected.All datasets in Sm Prot are free to access,and available for browse,search,and bulk downloads at http://bigdata.ibp.ac.cn/SmProt/. 展开更多
关键词 Ribosome profiling Small open reading frame Upstream open reading frame VARIANTS Disease
原文传递
上一页 1 2 下一页 到第
使用帮助 返回顶部