The purpose of this study is to investigate whether a near-infrared fluorescence(NIRF)probe,Cy5.5-d-glucosamine(Cy5.5-2DG),can image arthritis in collagen-induced arthritic(CIA)mice.The presence of arthritis was verif...The purpose of this study is to investigate whether a near-infrared fluorescence(NIRF)probe,Cy5.5-d-glucosamine(Cy5.5-2DG),can image arthritis in collagen-induced arthritic(CIA)mice.The presence of arthritis was verified by both visual examination and micro-computed tomography(MicroCT)imaging.CIA mice were imaged by a micro-positron emission tomography(MicroPET)scanner one hour after intravenous injection of 2-deoxy-2-[18F]fluoro-d-glucose([18F]FDG).After radioactivity of[18F]FDG decayed away,Cy5.5-2DG was injected into a lateral tail vein of the mice.Arthritic tissue targeting and retention of Cy5.5-2DG in CIA mice were evaluated and quantified by an optical imaging system.Inflammatory tissue in CIA mice was clearly visualized by[18F]FDG-MicroPET scan.NIRF imaging of Cy5.5-2DG in the same mice revealed that the pattern of localization of Cy5.5-2DG in the arthritic tissue was very similar to that of[18F]FDG.Quantification analysis further showed that[18F]FDG uptake in arthritic tissues at one hour post-injection(p.i.)and Cy5.5-2DG uptakes at different time points p.i.were all well correlated(r2 over 0.65).In conclusion,Cy5.5-DG can detect arthritic tissues in living mice.The good correlation between the[18F]FDG uptake and Cy5.5-2DG accumulation in the same arthritic tissue warrants further investigation of Cy5.5-2DG as an approach for assessment of anti-inflammatory treatments.展开更多
Follicular thyroid carcinoma(FTC)is the second most common form of thyroid malignancy,and it is associated with more aggressive growth and worse long-term survival outcomes relative to papillary thyroid carcinoma(PTC)...Follicular thyroid carcinoma(FTC)is the second most common form of thyroid malignancy,and it is associated with more aggressive growth and worse long-term survival outcomes relative to papillary thyroid carcinoma(PTC).Reliable approaches to preoperative FTC detection,however,remain to be established.Herein,a targeted Affibody-Au-Tripod nanoprobe was developed and successfully utilized to facilitate the targeted photoacoustic imaging(PAI)of epidermal growth factor receptor(EGFR)-positive cells and tumors.These Affibody-Au-Tripods were found to be highly sensitive and specific for cells expressing EGFR when used as a PA contrast agent in vitro,and studies conducted in an FTC-133 subcutaneous tumor model system in mice further revealed that these Affibody-Au-Tripods were able to specifically target these EGFR-expressing tumors while providing a strong photoacoustic signal in vivo.Importantly,these nanoprobes exhibited negligible cytotoxicity and robust chemical and physical stability,making Affibody-Au-Tripods promising candidates for targeted PAI-based FTC diagnosis.In addition,these nanoprobes have the potential to facilitate the individualized treatment of patients harboring EGFRpositive tumors.展开更多
During the past several decades,positron emission tomography（PET） has been one of the rapidly growing areas of medical imaging;particularly,its applications in routine oncological practice have been widely recognize...During the past several decades,positron emission tomography（PET） has been one of the rapidly growing areas of medical imaging;particularly,its applications in routine oncological practice have been widely recognized.At present,^18F-fluorodeoxyglucose（^18F-FDG） is the most broadly used PET probe.However,^18F-FDG also suffers many limitations.Thus,scientists and clinicians are greatly interested in exploring and developing new PET imaging probes with high affinity and specificity.In this review,we briefly summarize the representative PET probes beyond ^18F-FDG that are available for patients imaging in three major clinical areas（oncology,neurology and cardiology）,and we also discuss the feasibility and trends in developing new PET probes for personalized medicine.展开更多
Fluorescence imaging is capable of acquiring anatomical and functional infor- mation with high spatial and temporal resolution. This imaging technique has been indispensable in biological research and disease detectio...Fluorescence imaging is capable of acquiring anatomical and functional infor- mation with high spatial and temporal resolution. This imaging technique has been indispensable in biological research and disease detection/diagnosis. Imaging in the visible and to a lesser degree, in the near-infrared （NIR） regions below 900 nm, suffers from autofluorescence arising from endogenous fluorescent molecules in biological tissues. This autofluorescence interferes with fluorescent molecules of interest, causing a high background and low detection sensitivity. Here, we report that fluorescence imaging in the 1,500-1,700-nm region （termed ＂NIR-IIb＂） under 808-nm excitation results in nearly zero tissue autofluorescence, allowing for background-free imaging of fluorescent species in otherwise notoriously autofluorescent biological tissues, including liver. Imaging of the intrinsic fluorescence of individual fluorophores, such as a single carbon nanotube, can be readily achieved with high sensitivity and without autofluorescence background in mouse liver within the 1,500-1,700-nm wavelength region.展开更多
High quality InAs/InP/ZnSe core/shell/shell quantum dots have been grown by a one-pot approach.This engineered quantum dots with unique near-infrared(NIR)fluorescence,possessing outstanding optical properties,and the ...High quality InAs/InP/ZnSe core/shell/shell quantum dots have been grown by a one-pot approach.This engineered quantum dots with unique near-infrared(NIR)fluorescence,possessing outstanding optical properties,and the biocompatibility desired for in vivo applications.The resulting quantum dots have significantly lower intrinsic toxicity compared to NIR emissive dots containing elements such as cadmium,mercury,or lead.Also,these newly developed ultrasmall non-Cd containing and NIR-emitting quantum dots showed signifi cantly improved circulation half-life and minimal reticuloendothelial system(RES)uptake.展开更多
Stem cell therapy holds high promises in regenerative medicine.The major challenge of clinical translation is to precisely and quantitatively evaluate the in vivo cell distribution,migration,and engraftment,which cann...Stem cell therapy holds high promises in regenerative medicine.The major challenge of clinical translation is to precisely and quantitatively evaluate the in vivo cell distribution,migration,and engraftment,which cannot be easily achieved by current techniques.To address this issue,for the first time,we have developed a molecular cell tracker with a strong fluorescence signal in the second near-infrared(NIR-II)window(1,000-1,700 nm)for real-time monitoring of in vivo cell behaviors in both healthy and diseased animal models.The NIR-II tracker(CelTrac1000)has shown complete cell labeling with low cytotoxicity and profound long-term tracking ability for 30 days in high spatiotemporal resolution for semiquantification of the biodistribution of transplanted stem cells.Taking advantage of the unique merits of CelTrac1000,the responses of transplanted stem cells to different diseased environments have been discriminated and unveiled.Furthermore,we also demonstrate CelTrac1000 as a universal and effective technique for ultrafast real-time tracking of the cellular migration and distribution in a 100μm single-cell cluster spatial resolution,along with the lung contraction and heart beating.As such,this NIR-II tracker will shift the optical cell tracking into a single-cell cluster and millisecond temporal resolution for better evaluating and understanding stem cell therapy,affording optimal doses and efficacy.展开更多
基金National Natural Science Foundation of China(Grant No.90508003)National Cancer Institute(NCI)Small Animal Imaging Resource Program(SAIRP)Grant R24 CA93862NCI In Vivo Cellular Molecular Imaging Center(ICMIC)Grant P50 CA114747(SSG).
文摘The purpose of this study is to investigate whether a near-infrared fluorescence(NIRF)probe,Cy5.5-d-glucosamine(Cy5.5-2DG),can image arthritis in collagen-induced arthritic(CIA)mice.The presence of arthritis was verified by both visual examination and micro-computed tomography(MicroCT)imaging.CIA mice were imaged by a micro-positron emission tomography(MicroPET)scanner one hour after intravenous injection of 2-deoxy-2-[18F]fluoro-d-glucose([18F]FDG).After radioactivity of[18F]FDG decayed away,Cy5.5-2DG was injected into a lateral tail vein of the mice.Arthritic tissue targeting and retention of Cy5.5-2DG in CIA mice were evaluated and quantified by an optical imaging system.Inflammatory tissue in CIA mice was clearly visualized by[18F]FDG-MicroPET scan.NIRF imaging of Cy5.5-2DG in the same mice revealed that the pattern of localization of Cy5.5-2DG in the arthritic tissue was very similar to that of[18F]FDG.Quantification analysis further showed that[18F]FDG uptake in arthritic tissues at one hour post-injection(p.i.)and Cy5.5-2DG uptakes at different time points p.i.were all well correlated(r2 over 0.65).In conclusion,Cy5.5-DG can detect arthritic tissues in living mice.The good correlation between the[18F]FDG uptake and Cy5.5-2DG accumulation in the same arthritic tissue warrants further investigation of Cy5.5-2DG as an approach for assessment of anti-inflammatory treatments.
基金supported by the National Natural Science Foundation of China(81421004,81301268)Beijing Nova Program Interdisciplinary Cooperation Project (xxjc201812)+2 种基金International S&T Cooperation Program of China(2015DFA30440)Beijing Nova Program(Z131107000413063)CAMS Innovation Fund for Medical Sciences(CIFMS 2020-I2M-C&T-B-035)。
文摘Follicular thyroid carcinoma(FTC)is the second most common form of thyroid malignancy,and it is associated with more aggressive growth and worse long-term survival outcomes relative to papillary thyroid carcinoma(PTC).Reliable approaches to preoperative FTC detection,however,remain to be established.Herein,a targeted Affibody-Au-Tripod nanoprobe was developed and successfully utilized to facilitate the targeted photoacoustic imaging(PAI)of epidermal growth factor receptor(EGFR)-positive cells and tumors.These Affibody-Au-Tripods were found to be highly sensitive and specific for cells expressing EGFR when used as a PA contrast agent in vitro,and studies conducted in an FTC-133 subcutaneous tumor model system in mice further revealed that these Affibody-Au-Tripods were able to specifically target these EGFR-expressing tumors while providing a strong photoacoustic signal in vivo.Importantly,these nanoprobes exhibited negligible cytotoxicity and robust chemical and physical stability,making Affibody-Au-Tripods promising candidates for targeted PAI-based FTC diagnosis.In addition,these nanoprobes have the potential to facilitate the individualized treatment of patients harboring EGFRpositive tumors.
文摘During the past several decades,positron emission tomography（PET） has been one of the rapidly growing areas of medical imaging;particularly,its applications in routine oncological practice have been widely recognized.At present,^18F-fluorodeoxyglucose（^18F-FDG） is the most broadly used PET probe.However,^18F-FDG also suffers many limitations.Thus,scientists and clinicians are greatly interested in exploring and developing new PET imaging probes with high affinity and specificity.In this review,we briefly summarize the representative PET probes beyond ^18F-FDG that are available for patients imaging in three major clinical areas（oncology,neurology and cardiology）,and we also discuss the feasibility and trends in developing new PET probes for personalized medicine.
文摘Fluorescence imaging is capable of acquiring anatomical and functional infor- mation with high spatial and temporal resolution. This imaging technique has been indispensable in biological research and disease detection/diagnosis. Imaging in the visible and to a lesser degree, in the near-infrared （NIR） regions below 900 nm, suffers from autofluorescence arising from endogenous fluorescent molecules in biological tissues. This autofluorescence interferes with fluorescent molecules of interest, causing a high background and low detection sensitivity. Here, we report that fluorescence imaging in the 1,500-1,700-nm region （termed ＂NIR-IIb＂） under 808-nm excitation results in nearly zero tissue autofluorescence, allowing for background-free imaging of fluorescent species in otherwise notoriously autofluorescent biological tissues, including liver. Imaging of the intrinsic fluorescence of individual fluorophores, such as a single carbon nanotube, can be readily achieved with high sensitivity and without autofluorescence background in mouse liver within the 1,500-1,700-nm wavelength region.
基金This work was supported in part by the National Cancer Institute(NCI)(R21 CA121842,P50 CA114747,and U54 CA119367)the National Institute of Health(R43 EB005072)the National Science Foundation(CHE-0554812).
文摘High quality InAs/InP/ZnSe core/shell/shell quantum dots have been grown by a one-pot approach.This engineered quantum dots with unique near-infrared(NIR)fluorescence,possessing outstanding optical properties,and the biocompatibility desired for in vivo applications.The resulting quantum dots have significantly lower intrinsic toxicity compared to NIR emissive dots containing elements such as cadmium,mercury,or lead.Also,these newly developed ultrasmall non-Cd containing and NIR-emitting quantum dots showed signifi cantly improved circulation half-life and minimal reticuloendothelial system(RES)uptake.
基金supported by the National Natural Science Foundation of China(31870991,81301160,and 82071976)Shanghai Pujiang Program(19PJ1411100)+4 种基金Shanghai Municipal Science and Technology Major Project,American Heart Association(AHA)Postdoctoral Fellowship Award(18POST34030106)Guangdong Innovative and Entrepreneurial Research Team Program(2019ZT08Y191)Science and Technology Innovation Committee of Shenzhen Municipality(JCYJ20190809154011696)Stanford University,Department of RadiologyWe also would like to thank Dr.Andrew Olsen from Stanford Neuroscience Microscopy Service(NIH NS069375)on the support of confocal imaging of iPSC-ECs.
文摘Stem cell therapy holds high promises in regenerative medicine.The major challenge of clinical translation is to precisely and quantitatively evaluate the in vivo cell distribution,migration,and engraftment,which cannot be easily achieved by current techniques.To address this issue,for the first time,we have developed a molecular cell tracker with a strong fluorescence signal in the second near-infrared(NIR-II)window(1,000-1,700 nm)for real-time monitoring of in vivo cell behaviors in both healthy and diseased animal models.The NIR-II tracker(CelTrac1000)has shown complete cell labeling with low cytotoxicity and profound long-term tracking ability for 30 days in high spatiotemporal resolution for semiquantification of the biodistribution of transplanted stem cells.Taking advantage of the unique merits of CelTrac1000,the responses of transplanted stem cells to different diseased environments have been discriminated and unveiled.Furthermore,we also demonstrate CelTrac1000 as a universal and effective technique for ultrafast real-time tracking of the cellular migration and distribution in a 100μm single-cell cluster spatial resolution,along with the lung contraction and heart beating.As such,this NIR-II tracker will shift the optical cell tracking into a single-cell cluster and millisecond temporal resolution for better evaluating and understanding stem cell therapy,affording optimal doses and efficacy.
