Contrasting Transport Performance of Electron-and Hole-Doped Epitaxial Graphene for Quantum Resistance Metrology

Epitaxial graphene grown on silicon carbide(Si C/graphene)is a promising solution for achieving a highprecision quantum Hall resistance standard.Previous research mainly focused on the quantum resistance metrology of n-type Si C/graphene,while a comprehensive understanding of the quantum resistance metrology behavior of graphene with different doping types is lacking.Here,we fabricated both n-and p-type Si C/graphene devices via polymer-assisted molecular adsorption and conducted systematic magneto-transport measurements in a wide parameter space of carrier density and temperature.It is demonstrated that n-type devices show greater potential for development of quantum resistance metrology compared with p-type devices,as evidenced by their higher carrier mobility,lower critical magnetic field for entering quantized Hall plateaus,and higher robustness of the quantum Hall effect against thermal degeneration.These discrepancies can be reasonably attributed to the weaker scattering from molecular dopants for n-type devices,which is further supported by the analyses on the quantum interference effect in multiple devices.These results enrich our understanding of the charged impurity on electronic transport performance of graphene and,more importantly,provide a useful reference for future development of graphene-based quantum resistance metrology.

Determination of related substances in disulfide-rich peptide ziconotide by establishing tandem mass spectrometry methods

Currently,several disulfide-rich peptides have been officially approved as therapeutic medicines,such as w-conotoxin ziconotide[1],guanosine analogue linaclotide,and plecanatide.Peptides are predisposed to generate related substances(also termed as structurally related impurities)including amino acid deletion/insertion,diastereoisomerization,deamination/amination,oxidation,and succinimidation etc.

Research on perturbation of neutron fluence rate in a closed thermal neutron field due to medium materials

The neutron radiation field has vital applications in areas such as biomedicine,geology,radiation safety,and many others for neutron detection and neutron metrology.Correcting neutron fluence rate perturbation accurately is an important yet challenging problem.This study proposes a correction method that analyzes three physical processes.This method,which transforms the detection process from point detection to area detection,is based on a novel physical model and has been validated through theoretical analyses,experiments,and simulations.According to the average differences between the calculated and experimental results,the new method(1.67%)demonstrated better accuracy than the traditional simulation(2.17%).In a closed thermal neutron radiation field,the detector or strong neutron absorption material significantly perturbs the neutron fluence rate,whereas its impact on the energy spectrum shape and neutron directionality is relatively minor.Furthermore,based on the calculation results of the perturbation rate formula for medium materials with different compositions and sizes,the larger the volume and capture cross section of the medium,the higher the perturbation rate generated in the closed radiation field.

Bioengineering breakthroughs:The impact of stem cell models on advanced therapy medicinal product development

The burgeoning field of bioengineering has witnessed significant strides due to the advent of stem cell models,particularly in their application in advanced therapy medicinal products(ATMPs).In this review,we examine the multifaceted impact of these developments,emphasizing the potential of stem cell models to enhance the sophistication of ATMPs and to offer alternatives to animal testing.Stem cell-derived tissues are particularly promising because they can reshape the preclinical landscape by providing more physiologically relevant and ethically sound platforms for drug screening and disease modelling.We also discuss the critical challenges of reproducibility and accuracy in measurements to ensure the integrity and utility of stem cell models in research and application.Moreover,this review highlights the imperative of stem cell models to align with regulatory standards,ensuring using stem cells in ATMPs translates into safe and effective clinical therapies.With regulatory approval serving as a gateway to clinical adoption,the collaborative efforts between scientists and regulators are vital for the progression of stem cell applications from bench to bedside.We advocate for a balanced approach that nurtures innovation within the framework of rigorous validation and regulatory compliance,ensuring that stem cell-base solutions are maximized to promote public trust and patient health in ATMPs.

The development,performances and applications of the monochromatic X-rays facilities in(0.218–301)keV at NIM,China

Space scientific exploration is rapidly becoming the primary battlefield for humankind to explore the universe.Countries worldwide have launched numerous space exploration satellites.Accurate calibration of the detectors on the ground is a crucial element for space science satellites to obtain observational results.For the purpose of providing calibration for various satellite-borne detectors,multiple monochromatic X-rays facilities have been built at the National Institute of Metrology,P.R.China(NIM).These facilities mainly pertain to grating diffraction and Bragg diffraction,and the energy range of the produced monochromatic X-rays is 0.218–301 ke V.These facilities have a high performance in terms of energy stability,monochromaticity,and flux stability.The monochromaticity was greater than 3.0%.The energy stability of the facility is 0.02%at 25 ke V over 8 h,and the flux stability was within 1.0%at 25 ke V over 8 h.Calibration experiments on the properties of satellite-borne detectors,such as energy linearity,energy resolution,detection efficiency,and temperature response,can be conducted at the facilities.Thus far,the calibration of two satellites has been completed by the authors,and the work on three other satellites is in progress.This study will contribute to the advancement of X-ray astronomy the development of Chinese space science.

First Evaluation and Frequency Measurement of the Strontium Optical Lattice Clock at NIM

An optical lattice clock based on 87Sr is built at National Institute of Metrology （NIM） of China. The systematic frequency shifts of the clock are evaluated with a total uncertainty of 2.3×10-16. To measure its absolute frequency with respect to NIM＇s cesium fountain clock NIM5, the frequency of a flywheel H-maser of NIM5 is transferred to the Sr laboratory through a 50-kin-long fiber. reference frequency of this H-maser, is used for the optical this Sr clock is measured to be 429228004229873.7（1.4）Hz. A fiber optical frequency comb, phase-locked to the frequency measurement. The absolute frequency of

Decreased vibrational susceptibility of Fabry-Perot cavities via designs of geometry and structural support

Ultra-stable optical cavities are widely used for laser frequency stabilization. In these experiments the laser performance relies on the length stability of the Fabry-Perot cavities. Vibration-induced deformation is one of the dominant factors that affect the stability of ultra-stable optical cavities. We have quantitatively analysed the elastic deformation of Fabry-Perot cavities with various shapes and mounting configurations. Our numerical result facilitates a novel approach for the design of ultra-stable cavities that are insensitive to vibrational perturbations. This approach can be applied to many experiments such as laser frequency stabilization, high-precision laser spectroscopy, and optical frequency standards.

Photoluminescence and thermoluminescence properties of dysprosium doped zinc metaborate phosphors

Polycrystalline powder samples of dysprosium doped Zn（BO2）2 phosphors were prepared by solid state reaction in air at high temperature and characterized by X-ray powder diffraction. The IR, Photoluminescence （PL）, diffuse reflectance and three-dimensional （3D） thermoluminescence （TL） emission spectrum after 60^Co gamma ray irradiation were investigated. The characteristic 3D TL emission bands at about 425, 481 and 573 um with a main emission band of around 573 um were attributed to the host emission, 4^F9/2→6H15/2 and F9/2→6^H13/2 f-f transitions of Dy^3＋ ions. No emission from Dy^2＋ ions was observed in the measurement wavelength range. The TL-dose response of the Zn（BO2）2：Dy polycrystalline powder sample to gamma ray radiation in the range from 1 to 100 Gy at clinical dose levels was almost linear. The experimental results showed that Zn（BO2）2：Dy had potential use as the materials of gamma-ray thermoluminescence dosimeter （TLD） for clinical dosimetry.

Fabrication of Triplexers Based on Flattop SOI AWG

A triplexer is fabricated based on SOI arrayed waveguide gratings （AWGs）. Three wavelengths of the triplexer operate at different diffraction orders of an arrayed waveguide grating. The signals of 1490nm and 1550nm, which are input from central input waveguide of an AWG, are demultiplexed and the signal of 131Onto, which is input from central output waveguide of an AWG, is uploaded. The tested results show that the downloaded and uploaded signals have fiat-top response. The insertion loss is 9 dB on chip, the nonadjacent crosstalk is less than -30 dB for 1490nm and 1301 nm, and is less than -25 dB for 1550nm, the 3dB bandwidth equates that of the input light source.

Global characterization of modifications to the charge isomers of IgG antibody

Posttranslational modifications of antibody products affect their stability,charge distribution,and drug activity and are thus a critical quality attribute.The comprehensive mapping of antibody modifications and different charge isomers(CIs)is of utmost importance,but is challenging.We intended to quantitatively characterize the posttranslational modification status of CIs of antibody drugs and explore the impact of posttranslational modifications on charge heterogeneity.The CIs of antibodies were fractionated by strong cation exchange chromatography and verified by capillary isoelectric focusing-whole column imaging detection,followed by stepwise structural characterization at three levels.First,the differences between CIs were explored at the intact protein level using a top-down mass spectrometry approach;this showed differences in glycoforms and deamidation status.Second,at the peptide level,common modifications of oxidation,deamidation,and glycosylation were identified.Peptide mapping showed nonuniform deamidation and glycoform distribution among CIs.In total,10 N-glycoforms were detected by peptide mapping.Finally,an in-depth analysis of glycan variants of CIs was performed through the detection of enriched glycopeptides.Qualitative and quantitative analyses demonstrated the dynamics of 24 N-glycoforms.The results revealed that sialic acid modification is a critical factor accounting for charge heterogeneity,which is otherwise missed in peptide mapping and intact molecular weight analyses.This study demonstrated the importance of the comprehensive analyses of antibody CIs and provides a reference method for the quality control of biopharmaceutical analysis.

Precise determination of characteristic laser frequencies by an Er-doped fiber optical frequency comb

Femtosecond optical frequency combs correlate the microwave and optical frequencies accurately and coherently.Therefore,any optical frequency in visible to near-infrared region can be directly traced to a microwave frequency.As a result,the length unit“meter”is directly related to the time unit“second”.This paper validates the capability of the national wavelength standards based on a home-made Er-doped fiber femtosecond optical frequency comb to measure the laser frequencies ranging from visible to near-infrared region.Optical frequency conversion in the femtosecond optical frequency comb is achieved by combining spectral broadening in a highly nonlinear fiber with a single-point frequencydoubling scheme.The signal-to-noise ratio of the beat notes between the femtosecond optical frequency comb and the lasers at 633,698,729,780,1064,and 1542 nm is better than 30 d B.The frequency instability of the above lasers is evaluated by using a hydrogen clock signal with a instability of better than 1×10^(-13)at 1-s averaging time.The measurement is further validated by measuring the absolute optical frequency of an iodine-stabilized 532-nm laser and an acetylenestabilized 1542-nm laser.The results are within the uncertainty range of the international recommended values.Our results demonstrate the accurate optical frequency measurement of lasers at different frequencies using the femtosecond optical frequency comb,which is not only important for the precise and accurate traceability and calibration of the laser frequencies,but also provides technical support for establishing the national wavelength standards based on the femtosecond optical frequency comb.

Improved Evaluation of BBR and Collisional Frequency Shifts of NIM-Sr2 with 7.2×10^(-18)Total Uncertainty

NIM-Sr2 optical lattice clock has been developed on the Changping campus of National Institute of Metrology(NIM).Considering the limitations in NIM-Sr1,several improved parts have been designed including a differential pumping stage in the vacuum system,a permanent magnet Zeeman slower,water-cooled anti-Helmholtz coils,an extended viewport for Zeeman slower,etc.A clock laser with a short-time stability better than 3×10^(-16)is realized based on a self-designed 30-cm-long ultra-low expansion cavity.The systematic frequency shift has been evaluated to an uncertainty of 7.2×10^(-18),with the uncertainty of BBR shift and the collisional frequency shift being an order of magnitude lower than the last evaluation of NIM-Sr1.

Magic Wavelength Measurement of the ^(87)Sr Optical Lattice Clock at NIM

We report on the magic wavelength measurement of our optical lattice clock based on fermion strontium atoms at the National Institute of Metrology （NIM）. A Ti：sapphire solid state laser locked to a reference cavity inside a temperature-stabilized vacuum chamber is employed to generate the optical lattice. The laser frequency is measured by an erbium fiber frequency comb. The trap depth is modulated by varying the lattice laser power via an acousto-optic modulator. We obtain the frequency shift coefficient at this lattice wavelength by measuring the diffbrential frequency shift of the clock transition of the strontium atoms at different trap depths, and the frequency shift coefficient at this lattice wavelength is obtained. We measure the frequency shift coefficients at different lattice frequencies around the magic wavelength and linearly fit the measurement data, and the magic wavelength is calculated to be 368554672（44）MHz.

Study of Nb/Nb_xSi_(1-x)/Nb Josephson junction arrays

Owing to the adjustable characteristics and superior etching properties of co-sputtered Nbx Si1-x film, we are trying to fabricate Nb/Nbx Si1-x/Nb Josephson junction arrays for voltage standard. It is important to find the suitable Nbx Si1-x barrier for the junctions. Josephson junctions with different barrier content are fabricated. Current–voltage characteristics are measured and analyzed. It is demonstrated in this paper that critical current can be adjusted by using different barrier content and thickness. Shapiro steps of five hundred junctions in series are observed.

Vehicle kinematics modeling and design of vehicle trajectory generator system

A trajectory generator based on vehicle kinematics model was presented and an integrated navigation simulation system was designed.Considering that the tight relation between vehicle motion and topography,a new trajectory generator for vehicle was proposed for more actual simulation.Firstly,a vehicle kinematics model was built based on conversion of attitude vector in different coordinate systems.Then,the principle of common trajectory generators was analyzed.Besides,combining the vehicle kinematics model with the principle of dead reckoning,a new vehicle trajectory generator was presented,which can provide process parameters of carrier anytime and achieve simulation of typical actions of running vehicle.Moreover,IMU(inertial measurement unit) elements were simulated,including accelerometer and gyroscope.After setting up the simulation conditions,the integrated navigation simulation system was verified by final performance test.The result proves the validity and flexibility of this design.

Development of 0.5-V Josephson junction array devices for quantum voltage standards

The design, fabrication, and the characterization of a 0.5-V Josephson junction array device are presented for the quantum voltage standards in the National Institute of Metrology(NIM) of China. The device consists of four junction arrays, each of which has 1200 3-stacked Nb/NbxSi1-x/Nb junctions and an on-chip superconducting microwave circuit which is mainly a power divider enabling each Josephson array being loaded with an equal amount of microwave power. A direct current(dc) quantum voltage of about 0.5 V with a ~1-mA current margin of the 1 st quantum voltage step is obtained.To further prove the quality of NIM device, a comparison between the NIM device with the National Institute of Standards and Technology(NIST) programmable Josephson voltage standard(PJVS) system device is conducted. The difference of the reproduced 0.5-V quantum voltage between the two devices is about 0.55 nV, which indicates good agreement between the two devices. With the homemade device, we have realized a precise and applicable 0.5-V applicable-level quantum voltage.

Comparison of neutron energy spectrum unfolding methods and evaluation of rationality criteria

The neutron energy spectrum was measured using a Bonner sphere spectrometer at six locations inside the containment vessel of a nuclear reactor at the Qinshan nuclear power plant. The structures of the neutron spectra obtained by the maximum entropy, iteration, and genetic algorithm methods were consistent with one another and could be interpreted as the spectral superposition of different energy regions. The characteristic parameters of the neutron spectrum, including the fluence rate,average energy, and neutron ambient dose equivalent rate H^(*)(10), were in good agreement among the three methods. In addition, an LB6411 neutron ambient dose equivalent meter was employed to obtain the H^(*)(10) directly for comparison.These findings indicate that neutron spectrum unfolding methods can be used to overcome the problems associated with the response functions of dosimeters to provide more accurate H^(*)(10) values. In this study, the following three evaluation criteria were systematically addressed to ensure the accuracy of the unfolded spectra: count rates of the inverse solutions,neutron spectrum structures, and comparison of key parameters.

Model eyes with curved multilayer structure for the axial resolution evaluation of an ophthalmic optical coherence tomography device

Optical coherence tomography(OCT)has been widely applied to the diagnosis of eye diseasesduring the past two decades.However,valid evaluation methods are stil not available for theclinical OCT devices.In order to assess the axial resolution of the OCT system,standard modeleyes with micro-scale multilayer structure have been designed and manufactured in this study.Mimicking a natural human eye,proper Titanium dioxide(TiO_(2))materials of particles withdifferent concentrations were selected by testing the scattering coefficient of PDMS phantoms.The artificial retinas with multilayer films were fabricated with the thicknesses from 9.5 to 30 micrometers using spin coating technology,Subsequently,standard OCT model eyes were ac-complished by embedding the retina phantoms into the artificial frames of eyes.For ease ofmeasurement processing,a series of model eyes were prepared,and each contained flms withthree kinds of thicknesses.Considering the traceability and accuracy of the key parameters of the standard model eyes,the thicknesses of multilayer structures were verfed using ThicknessMonitoring System.Through the experiment with three different OCT devices,it demonstratedthe model eyes fabricated in this study can provide an effective evaluation method for the axialresolution of an ophthalmic OCT device.

Characterization of a nano line width reference material based on metrological scanning electron microscope

The line width(often synonymously used for critical dimension,CD)is a crucial parameter in integrated circuits.To accurately control CD values in manufacturing,a reasonable CD reference material is required to calibrate the corresponding instruments.We develop a new reference material with nominal CDs of 160 nm,80 nm,and 40 nm.The line features are investigated based on the metrological scanning electron microscope which is developed by the National Institute of Metrology(NIM)in China.Also,we propose a new characterization method for the precise measurement of CD values.After filtering and leveling the intensity profiles,the line features are characterized by the combination model of the Gaussian and Lorentz functions.The left and right edges of CD are automatically extracted with the profile decomposition and k-means algorithm.Then the width of the two edges at the half intensity position is regarded as the standard CD value.Finally,the measurement results are evaluated in terms of the sample,instrument,algorithm,and repeatability.The experiments indicate efficiency of the proposed method which can be easily applied in practice to accurately characterize CDs.

Walking of spider on water surface studied from its leg shadows

Different from sculling forward of water striders with their hairy water-repellent legs, water spiders walked very quickly on water surfaces. By using a shadow method, the walking of water spiders had been studied. The three-dimensional trajectories and the supporting forces of water spider legs during walking forward were achieved. Results showed that the leg movement could be divided into three phases： slap, stroke, and retrieve. Employing an effective strategy to improving walking efficiency, the sculling legs supported most of its body weight while other legs were lifted to reduce the lateral water resistance, which was similar to the strategy of water striders. These findings could help guiding the design of water walking robots with high efficiency.