Ovarian Vein Thrombosis, Critical Diagnosis at Al-Hasahisa Obstetrics & Gynecology Teaching Hospital in Geziera State, Sudan

Ovarian vein thrombosis (OVT) is a rare thrombotic condition commonly attributed to the puerperium. The complications of OVT can be significant, and diagnosis relies on a high clinical index of suspicion. It can occur with lower quadrant pain that may mimic a surgical abdomen, especially in the setting of recent pregnancy, abdominal surgery, inflammatory disease, or malignancy. Diagnosis can be made with confidence using ultrasound, computed tomography, or magnetic resonance imaging. Management of OVT is particularly important in postpartum patients, with anticoagulation therapy being the treatment of choice.

Erratum to “Routine Episiotomy Practice at a Tertiary Care Center in Saudi Arabia” [Open Journal of Obstetrics and Gynecology 6 (2016) 794-797]

The original online version of this article (Oraif, A. (2016) Routine Episiotomy Practice at a Tertiary Care Center in Saudi Arabia. Open Journal of Obstetrics and Gynecology, 6, 794-797. http://dx.doi.org/10.4236/ojog.2016.613097) was published without the coauthors mistakenly. To reflect the contribution and responsibility of the coauthors at the time of the study, we revised authorship and affiliation of the article.

Quality Appraisal of Clinical Guidelines in Obstetrics and Gynecology in India

Background: Clinical practice guidelines are important for standard of care. This study was undertaken to assess the quality of Obstetrics and Gynecology guidelines in India. Objective Systematic appraisal of clinical practice guidelines (CPG) in obstetrics and gynecology in India uses Appraisal of Guidelines for Research and Evaluation II (AGREE II) instrument. Methods: All reported Indian guidelines in obstetrics and gynecology from 1st Jan. 2000 to 1st April 2018 were identified and subjected to inclusion using 3-point assessment criteria (relevance, clarity of intervention/outcome, and appropriate use of healthcare resources) using WHO AGREE-II instrument. Separate scores for the mean item, domain, and standardized were calculated by averaging the scores across two appraisers. Results & Discussion: Of 47 Clinical Practice Guidelines in Obstetrics and Gynecology (CPG) from India, 8 met the inclusion criteria. These were assessed using AGREE II checklist. Overall scores ranged from 8% to 22% (median 5%). No guideline received “Yes” for clinical use by either of the appraisers. Scores for scope and purpose and clarity were high but were very low for all the other domains. Only 1 review had identified cost as one of the focus areas as part of the guideline. Conclusion: Though being clinically relevant the clinical practice guidelines related to obstetrics and gynecology in India score poorly. There is a need for sensitization and capacity building of clinicians and public health professionals on the development of CPG related to obstetrics and gynecology in India.

Emergency Obstetric Hysterectomy in a Low Resources Country: An Eight-Year Retrospective Cohort Study from a Secondary Care Hospital in Niger

Introduction: Emergency obstetric hysterectomy (EOH) is a lifesaving procedure that is performed as a last resort in cases of severe postpartum haemorrhage. Objective: The objective of this study was to determine the incidence, socio-demographic profile of patients, indications, management and maternal-fetal outcomes of EOH in a maternity hospital with limited resources in Niger. Methodology: This was an eight-year retrospective cohort study involving the analysis of medical records from patients who underwent emergency obstetric hysterectomies between 1 January 2015 and 31 December 2022 at the Mother and Child Health Centre (MCHC) in Maradi, Niger. The epidemiological data, indications, and outcomes of EOH were collated and subjected to analysis using the statistical software package SPSS 21.0. Comparisons were made using the Chi-squared test. A p-value of less than 0.05 was considered statistically significant. Results: During the study period, 239 cases of emergency obstetric hysterectomy were recorded out of 269,710 deliveries, representing a frequency of 0.89%. The mean age of the patients was 32.41 years (range: 17 - 50 years). The patients were identified as married (239 cases, 100%), unemployed (228 cases, 95.4%), and not attending school (215 cases, 90%). The largest number of cases were observed in large multiparous women (i.e., those with more than five children), representing 58.6% of the total number of cases (140 patients). The average parity among this group was 6.15 children. The majority of patients (229 patients, 95.82%) had undergone in utero transfer, with 169 patients (70.71%), originating from peripheral maternity units in the Maradi region. Upon admission, 116 patients (48.53%) exhibited active genital haemorrhage, while 58 patients (24.26%) were in shock. The primary indications for hysterectomy were uterine rupture (153 patients, 64%), uterine atony (77 patients, 32.2%), and placental accreta (six patients, 2.5%). Hysterectomy was performed by an obstetric gynaecologist (230 patients, 96.2%), an obstetric gynaecology resident (six patients, 2.5%), or a general practitioner with district surgical expertise (three patients, 1.3%). In the majority of cases (180 patients, 75.3%), total hysterectomy was performed. The incidence of maternal mortality (26 patients, 10.9%), and perinatal mortality (223 newborns, 93.4%) was notably elevated in our series. Conclusion: Our findings are in close alignment with those previously documented in the literature. The practice of emergency obstetric hysterectomy is a common occurrence in our region. It is considered a last resort when conservative procedures have failed or are not an option. Improved obstetric management would result in a reduction in the number of cases of haemostatic hysterectomy. The availability of blood products is expected to improve maternal prognosis.

Educational Program for Pregnant Women Regarding Obstetrics Dangerous Signs in Rural Areas

Background: Complications linked to postpartum & pregnancy are one of the major causes of female death. In order to understand the causes of complications and encourage women to take sufficient action in order to receive emergency treatment, a critical step should therefore be taken to minimize complications related to pregnancy, in order to ensure the safety of both women and newborns. Aim of the work: Evaluate the effectiveness of the education programs for pregnant women on obstetric danger signs in rural areas and help minimize the incidence of pregnancy loss and comorbidities. Methods: A quasi-experimental design on 70 women from a population of through 372 women in six-month in antenatal clinics recruited from the previously mentioned settings with pre- and post-test was conducted at antenatal clinics (M.C.H centers) affiliated to the available geographical health zones in EL-fayoum rural area including: Al-sheikh hassan at sanorse. We collected the data of women characteristics by a self-administered interview questionnaire & a structured reported knowledge and practices checklist to evaluate pregnant women practices and knowledge. Follow up was done to the studied groups & histopathology assessments of the product of conception in cases of abortion secondary to the complications to compare between effect of antenatal care program on the woman who followed the program and those who did not. Results: There is an improvement in 63% of pregnant women knowledge and practices after educational program in all aspects. The most common danger signs that may occur during pregnancy were miscarriage and vaginal bleeding, intrauterine fetal death as reported by women & confirmed by the histopathology reports. Conclusion: The educational program had an efficient improving women knowledge and practice regarding danger signs for pregnant women in rural areas, with highly statically significant differences in all the tested items between pre/post program implementation (P < 0.001). Recommendations: Establishment of in-service training programs and continuous supervision in rural areas to a raise women knowledge and practice regarding educational pregnant women and developing antenatal classes for all pregnant women about obstetric danger signs.

Is There a Relationship between COVID-19 Infection and the Occurrence of Anencephaly? Observation of the Maternity of Mohammed the VI University Hospital of Marrakech

Introduction: Morocco has experienced, like the whole world, the COVID-19 pandemic. Until the writing of this article, a subjective observation of the increase in the number of anencephaly has been observed in our facility. And since the teratogenic potential of the SARS Cov 2 virus is not sufficiently documented, we wondered if there would be a relationship between the relatively high number of anencephalic fetuses and COVID-19 infection. Aim: the objective of the study is to look for a possible correlation between the period of conception of these anencephalies and the pandemic. Material and methods: This is a retrospective study (from June 1, 2020 to May 31, 2022 in the Obstetrics Gynecology department, Faculty of Medicine and Pharmacy, CADI AYYAD UNIVERSITY Marrakech Morocco) consisting of the analysis of the number of patients in the target embryonic period (namely the closure of the neural tube: D18 to D30 of embryonic life) compared to the reference curves of the evolution of the SARS Cov2 pandemic. Results: The analysis of the number of patients at the target embryonic phase shows that many of our anencephalons (63%) were conceived during periods of high diffusion of the SARS Cov 2 virus in Morocco. Conclusion: Our study suggests a relationship between the COVID-19 pandemic and the occurrence of anencephaly, we cannot, however, highlight the type of direct or indirect relationship that binds them. Therefore, further studies should be considered to better investigate this relationship.

Burden of Congenital Defects Diagnosed through Ultrasonography in Soba Fetomaternal Unit, Khartoum, Sudan

Background: Congenital anomalies are among the leading causes of fetal loss, despite it can be identified prior to birth through advanced technology in expert hands. Our research aimed at estimating the prevalence of congenital anomalies in Sudan. Methods: A facility-based retrospective cross-sectional study combined with a community-based survey through a telephone interview was implemented on a purposive convenient sample of 138 participants. The data were computerized in Epi Info 7. Google Earth Pro enabled to collect the geographical coordinates for the residence of the participants. Descriptive statistics were performed through SPSS 23 and ArcGIS 10.3 was used to generate the geographical distribution map of congenital defects to visualize the catchment areas of Soba Ultrasonography Unit. Results: Of the 138 participants, the estimated prevalence of congenital defects was 2.2/10,000 live births. The ultrasonography screening revealed that neural tube defects were the most prevalent anomalies with 13.0% (18/138), which represented 47.4% (18/38) of all defects. Concerning children, a mortality rate of 23.2% (32/138) was reported. Conclusions: The child mortality rate post ultrasound screening of 23.2%, and the neural tube defects being the most common anomalies appealed to Sudan health authorities for focusing on more preventive antenatal practices to strengthen and promote maternal and child health.

Effect of Health Education Program on Self-Efficacy and Functional Capacity of Patients with Coronary Artery Bypass Surgery in Khartoum State-Sudan, 2015-2019

Coronary heart disease is among the most prevalent and costly of all global health problems. Coronary artery bypass grafting (CABG) has been increasingly used since the 1960s. Coronary heart disease is a leading cause of death worldwide, including Sudan. Self-efficacy and functional capacity is a critical factor for quality of life in patients who has undergone CABG as well as for their caregivers from their families. Aim of the Study: To explore the effects of an educational program on self-efficacy and functional capacity of patients after CABG. 1) There will be no significant difference between the intervention group and control group concerning knowledge regarding Self-efficacy and functional capacity post CABG surgery. 2) The application of our educational program for the patients will enhance the patients with CABG surgery and improve knowledge about Self-efficacy and functional capacity post CABG surgery. 3) There are some socio demographic factors that affect the response to the educational program. Methods: This was a quasi-experimental, interventional and hospital-based study, including pre-test, a post-test and follow-up test, with a control group, conducted during the period from September 2014 to June 2019. Patients were recruited consecutively to the cases and control group using convenience sampling. The sample consisted of 76 patients;41 as cases and 35 as control group. The sample included those who had undergone CABG in Khartoum State. Relevant data were collected using a checklist to monitor the effect of a health education program on self-efficacy and functional capacity of the patients. Result: The results showed marked improvement in patients’ self-efficacy and functional capacity between pretest and posttest for the study group;p-value = (0.001) in the health status of cases in comparison to the control group. The indices were observed to be higher for patients who received a nursing educational program throughout the study period;p-value = (0.002).

妊娠合并糖尿病诱发胚胎先天性神经管缺陷动物模型的MAP激酶信号传导机制

为了揭示妊娠合并糖尿病诱发胚胎先天性神经管缺陷的分子机制,并探讨其有效的防治方法。实验选用6个实验组的Sprague Dawley大鼠:第1组为常规饲养的正常对照组;第2组尾静脉注射65mg/kgStreptozotocin(STZ)构建妊娠合并糖尿病、且诱发先天性神经管缺陷的实验组大鼠;第3组为STZ构建的糖尿病、但胚胎不伴有先天性神经管缺陷的大鼠模型;第4、5、6组为STZ构建的糖尿病治疗组大鼠,每日分别给予80μg/mL花生四烯酸(arachidonicacid,AA)、400mg维生素E、抗氧化剂(维生素E)和不饱和脂肪酸(safloweroil)混合物cocktail治疗。于妊娠第12天取出各组胚胎,解剖显微镜下进行形态学分析;提取卵黄囊细胞蛋白质,应用特异性抗磷酸化抗体进行免疫共沉淀及Western印迹,对MAP激酶信号途径上各蛋白激酶ERK1/2、JNK1/2、RAF 1活性进行分析。与正常对照组相比,妊娠合并糖尿病诱发的先天性神经管缺陷胚胎中(第2组),ERK1/2蛋白激酶活性显著下降,其上游RAF 1活性相应降低;与此相反,JNK1/2活性明显升高。在给予花生四烯酸、维生素E补充物治疗后,通过调节MAP激酶信号通路蛋白激酶活性,逆转了胚胎神经管缺陷的发生。妊娠合并糖尿病诱发的胚胎先天性神经管缺陷的发生,与MAP激酶信号传导机制异常密切相关。

妊娠合并糖尿病诱发胚胎先天性神经管缺陷的MAP激酶及细胞凋亡信号传导机制

目的 :探讨妊娠合并糖尿病诱发胚胎先天性神经管缺陷的分子机制。方法 :将雌性SD大鼠分为 3组 :第 1组为正常对照组 ;第 2组为streptozotocin (STZ)构建的妊娠合并糖尿病诱发先天性神经管缺陷的实验组 ;第 3组为STZ构建的糖尿病大鼠模型 ,胚胎不伴有先天性神经管缺陷。于妊娠第 1 2天取出各组胚胎 ,解剖显微镜下进行形态学分析 ;对卵黄囊细胞进行DNA片段分析 ;提取卵黄囊细胞蛋白质 ,应用特异性抗磷酸化抗体进行免疫共沉淀及Westernblot对MAPkinase(MAPK)信号途径上各蛋白激酶活性进行分析。结果 :第 2组中 ,卵黄囊细胞出现细胞凋亡特征性的DNAladder,与正常对照组相比ERK1 /2蛋白激酶活性显著下降 ;与此相反 ,JNK1 /2活性明显升高 ,凋亡相关基因caspase 3、Bax高表达 ,凋亡抑制基因AKT活性明显受抑。结论 :糖尿病诱发的胚胎先天性神经管缺陷的发生 。

胎儿宫内窘迫与新生儿缺血缺氧性脑病的关系

胎儿窘迫是产科严重并发症，以胎儿缺氧、酸中毒为主要特征的症候群，可造成新生儿窒息及出生后永久性的神经系统损伤后遗症。对缺氧缺血患儿发生神经系统后遗症的早期预测及基于蛋白质组学的相关生物标记物发现，不但对急性期的诊断有益，而且对高危患儿的管理及早期干预有更深远的意义。本文就胎儿宫内窘迫的诊断、新生儿缺血缺氧性脑病（HIE）预测方法包括胎儿娩出后即取脐动脉血及脐静脉血血气分析了解酸中毒程度，脐静脉血检测促红细胞生成素（EPO），

重组小鼠Parkin共同调节基因在昆虫细胞系统中的表达

目的:构建小鼠Parkin co-regulated gene(Pacrg)/GFP-p Fast Bac1重组杆状病毒载体,并在Sf9昆虫细胞中表达。方法:PCR扩增小鼠全长Pacrg编码c DNA序列,通过TA克隆、连接等方法将该基因插入到携带e GFP的供体质粒p Fast Bac1中,获得重组载体rp FBac-Pacrg-GFP,然后转化到DH10Bac宿主菌中。筛选获得的重组杆状病毒载体r Bacmid-Pacrg-e GFP经脂质体转染至Sf9昆虫细胞中,荧光显微镜及Western印迹检测分析重组蛋白。结果:经测序及酶切鉴定显示构建的Pacrg/GFP-p Fast Bac1重组杆状病毒载体正确,Western印迹结果显示PACRG/e GFP融合蛋白在Sf9昆虫细胞中表达且携带绿色荧光。结论:成功构建了Pacrg/GFP-p Fast Bac1重组杆状病毒载体,且该重组蛋白在Sf9昆虫细胞中大量表达,为进一步研究PACRG蛋白的结构及其在精子发生中的调控作用奠定了基础。

Inhibition of PI3K/Akt/m TOR signaling pathway enhances the sensitivity of the SKOV3/DDP ovarian cancer cell line to cisplatin in vitro

The activation of the PI3K/AKT/m TOR pathway plays a key role in ovarian cancer tumorigenesis, progression and chemotherapy resistance. This study aimed to explore the possible mechanism that PI-103, a dual inhibitor of phosphatidylinositide 3-kinase and m TOR, enhances the sensitivity of SKOV3/DDP ovarian cancer cell line to cisplatin chemotherapy. The results showed that PI-103 could significantly increase the sensitivity of SKVO3/DDP cells to cisplatin through inhibiting the activation of PI3K/Akt/m TOR signaling pathway and inducing cell cycle arrest and apoptosis.

SUBSTITUTION OF ACUPUNCTURE FOR HCG IN OVULATION INDUCTION

By using human menopausal gonadotropin (HMG) and human chorionic gonadotropin (HCG), fairly good clinical therapeutic efficacy has been obtained in the treatment of infertility. However, difficulties are brought about due to the ovarian hyperstimulation syndrome (OHSS) easily induced by these two drugs. Therefore,

Differentiation of neuron-like cells from mouse parthenogenetic embryonic stem cells

Parthenogenetic embryonic stem cells have pluripotent differentiation potentials, akin to fertilized embryo-derived embryonic stem cells. The aim of this study was to compare the neuronal differentiation potential of parthenogenetic and fertilized embryo-derived embryonic stem cells. Before differentiation, karyotype analysis was performed, with normal karyotypes detected in both parthenogenetic and fertilized embryo-derived embryonic stem cells. Sex chromosomes were identified as XX. Immunocytochemistry and quantitative real-time PCR detected high expression of the pluripotent gene, Oct4, at both the mRNA and protein levels, indicating pluripotent differentiation potential of the two embryonic stem cell subtypes. Embryonic stern cells were induced with retinoic acid to form embryoid bodies, and then dispersed into single cells. Single cells were differentiated in N2 differentiation medium for 9 days. Immunocytochemistry showed parthenogenetic and fertilized embryo-derived embryonic stem cells both express the neuronal cell markers nestin, ~lll-tubulin and myelin basic protein. Quantitative real-time PCR found expression of neuregenesis related genes （Sox-1, Nestin, GABA, Pax6, Zic5 and Pitxl） in both types of embryonic stem cells, and Oct4 expression was significantly decreased. Nestin and Pax6 expression in parthenogenetic embryonic stem cells was significantly higher than that in fertilized embryo-derived embryonic stem cells. Thus, our experimental findings indicate that parthenogenetic embryonic stem cells have stronger neuronal differentiation potential than fertilized embryo-derived embryonic stem cells.

cDNA基因芯片技术检测妊娠合并糖尿病诱发胚胎先天性神经管缺陷表达差异基因(英文)

目的探讨妊娠合并糖尿病诱发胚胎先天性神经管缺陷的差异表达基因,揭示胚胎先天性神经管缺陷发生、发展的分子机制。方法选用2组的70～90天的SD大鼠:第1组(n=3)为接受常规饲养的正常对照组;第2组(n=3)为实验组:尾静脉注射65mg/kg链脲菌素(STZ),构建妊娠合并糖尿病诱发胚胎先天性神经管缺陷组大鼠动物模型。于妊娠第12天取出各组胚胎,解剖显微镜下进行神经管缺陷形态学判定。提取卵黄囊细胞的mRNA,以cDNA基因芯片技术对差异表达基因进行检测。结果对实验组及正常对照组卵黄囊细胞的1200个基因进行比较,共筛选出差异表达基因79条,其中42条表达上调基因(包括凋亡相关基因BAX、bcl 2、HSP70、glucose transporter3等),37条表达下调基因(包括phospholipaseA2、insulin likegrowthfactorIIreceptor等)。结论揭示了妊娠合并糖尿病诱发胚胎先天性神经管缺陷的差异表达基因,对先天性神经管缺陷有效的早期诊断和治疗提供了实验依据。

Subclinical Hypothyroidism and Isolated Hypothyroxinemia during Pregnancy and Their Association with Pregnancy Outcome: A 2-Year Study

INTRODUCTION: Overt hypothyroidism in pregnancy is associated with adverse outcomes, but the effects of subclinical hypothyroidism and isolated hypothyroxinemia on pregnancy outcomes are still controversial. Subclinical hypothyroidism might be associated with preterm delivery, low Apgar score, fetal death and miscarriages and isolated hypothyroxinemia with preterm labor, high birth weight and neurocognitive deficits in children. Other studies show no association with any complications at all. In the present study we tried to estimate whether maternal subclinical hypothyroidism and isolated hypothyroxinemia are associated with complications during pregnancy in our population which represents the pregnant population of Western Greece. METHODS: In a total of 469 pregnant women, third trimester serum was assayed for thyroid-stimulating hormone (TSH) and free thyroxine (free T4). Thyroid hypofunction was defined as 1) subclinical hypothyroidism with TSH levels above 3 mIU/L for the 3rd trimester and normal free T4 and 2) isolated hypothyroxinemia with TSH levels below 3 mIU/L for the 3rd trimester and free T4 below the 5th percentile. The results were associated with birth weight, week of labor, history of miscarriages and demographic characteristics. Patients with thyroid hypofunction were compared with euthyroid patients (TSH and free T4 between normal limits). The results were analyzed with one-way ANOVA and χ2 test. RESULTS: Subclinical hypothyroidism was documented in 10.9% and isolated hypothyroxinemia in 21.7% of pregnant women. Subclinical hypothyroidism was not associated with birth weight, week of labor, or history of miscarriages. On the contrary, hypothyroxinemia was associated with high birth weight (P < 0.05). CONCLUSION: In our study, we did not find a link between subclinical hypothyroidism and adverse pregnancy outcomes. Similarly, hypothyroxinemia was not associated with pregnancy complications. However, it was associated with higher birth weight. Our studies contribute to our understanding, whether or not subclinical hypothyroidism and isolated hypothyroxinemia are associated with pregnancy adverse outcomes and whether treatment and test for hypothyroidism should become routine during pregnancy.

Differential diagnosis of ambiguous genitalia in Chinese patients

Objective: To study the etiology and differential diagnos is of ambiguous genitalia.Methods: One hundred and five cases of ambiguous genitalia with disorders of sexual differentiation were analyzed based on clinical characterist ics and basic research. Results: Ambiguous genitalia are closely related to the functio n of androgen. It could be classified into 3 groups: androgen excess, androgen d eficiency and failure of gonadal differentiation. Differential diagnosis depends on clinical manifestations, chromosome examination, serum hormone determination s, final gonadal pathology, receptor study etc.Conclusion: Ambiguous genitalia are related to abnormal androgen ic effect and early correct etiological diagnosis is the key to its management.

A Study on the Traditional Chinese Medicine Jinyebaidu for Prevention and Treatment of Intrauterine Infection with Guinea Pigs Cytomegalovirus

The purpose is to study the prophylactic and therapeutic effect of the traditional Chinese Medicine （TCM）-Jinyebaidu （JYBD） to guinea pig cytomegalovirus （GPCMV） intrauterine infection. The virus-free female and male guinea pigs were screened with nest-polymerase chain reaction （N-PCR）. After inbred, pregnant guinea pigs were selected and divided into 3 groups randomly： 5 guniea pigs of the blank control group were not given either GPCMV or JYBD. 31 guniea pigs of the positive control group were inoculated 1 mL （107 TCID50 ） suspension of GPCMV intraperitoneal. 10 gunlea pigs of the experimental group were inoculated GPCMV firstly and then perfused stomach with JYBD for 14 days （Dosage in accordance with the modulus of the weight ratio of human to guniea pig）. The effects of JYBD on the intrauterine infection of GPCMV were observed. The results showed that JYBD could decrease the maternal infection rate from 100 % （31/31） to 50 % （5/10） （P〈0. 001）, the intrauterine infection rate from 100% （72/72） to 75 % （21/28） （P〈 0. 001）, and the rate of abnormal outcome of pregnancy from 64.4 % （29/45） to 25.0 % （7/28） （P〈0. 001）, the infective symptoms being relieved. It can be concluded that traditional Chinese medicine- JYBD can prevent and treat GPCMV intrauterine infection, and can be expected a prophylactic drug for HCMV intrauterine infection.

Integrative medicine:the guide line in women's reproductive health and the treatment of gynecological disease

Integrative medicine follows the principles in TCM that nature and human beings being correspondent with its holistic and dialectic views and explores the integrating point with theories and scientific data in modern medicine. During study on 16 gynecological diseases, the deficiency. In patients with pubertal uterine bleeding, ovulation is induced by acupuncture via decrease in central β-endorphin (β-EP) levels, which leads to the cure of anorexia nervosa by acupuncture via decrease in serum cortisol levels in early 1980s. These results in TCM lead to the idea that neuro-endocrine system is directly related to metabolic system which is also proved to be true in the treatment of polycystic ovary syndrome (PCOS) with or without hyperinsulinemia by two formulas (Yu's Tonifying Ricipe and Tian Gui Recipe,TGR) respectively according to differential diagnosis in TCM. Ovulation resulted with decrease in obesity, acanthosis nigricans, serum androgen, insulin, leptin and hyperthalamic neuropeptide Y (NPY), proopiomalanocortin (POMC) levels based on scientific data from both patients and an animal modal. It suggests that metabolic network, and the incidence of obesity, hypertension, diabetes and cancers of the endometrium and breast may be reduced among patients with PCOS in their future life while these recipes being taken long period. For perimenopausal syndrome, formula Geng Nian Chun (GNC)without increase in serum estrogen levels. In aging rats, it was found that GNC increased the ER and ERmRNA expressions in the ovarian axis and the spleen accompanying with increase in central β-EP, serotonin (5-HT) and peripheral interlutin-2 (IL-2) levels in aging rats without changes of estrogen levels. These data may refer to the release of symptoms in women with postmenopausal syndome. It suggests that GNC up-regulates the fading of neuro-endocrine-immune network. Prevention, alleviation of osteoporosis, cardiovascular disease and Alzheimer disease may be expected in aging women with long period taking GNC.