The Influence of Workplace Environment on Mental Health:A Quantitative and Qualitative Investigation in China

Background:The demanding nature of nursing,characterized by long hours,high-stress environments,and substantial workloads,can significantly impact nurses’mental health.However,there are limited studies that assessed the influence of workplace environment on nursing mental health based on both quantitative and qualitative approaches.Methods:This study aims to comprehensively investigate the multidimensional relationship between the workplace environment and nurses’well-being.This cross-sectional study was based on a sample of 3256 nurses from various healthcare settings in Shandong province,China(2022),who participated in the quantitative phase.For the qualitative phase,a subsample of participants from the quantitative phase was selected(n=200)using purposive sampling to ensure diversity in terms of age,gender,years of experience,and healthcare settings.The mean age was 35.2 years,with 74%female participants.Results:Significant negative correlations were found between leadership support and mental distress,while workload showed negative associations with General Positive Affect and mental health functioning(p<0.05).Multiple linear regression demonstrated the predictive power of workplace dimensions on mental health,with leadership support,teamwork,and organizational culture positively associated with mental health functioning(p<0.05).Thematic analysis underscored the intricate interplay between workplace indicators and mental health dimensions,emphasizing the need for a holistic approach.Conclusion:This study reveals that key workplace factors,including leadership support,teamwork,and organizational culture,play a vital role in enhancing nurse mental health,which is essential for sustaining high-quality patient care and a resilient healthcare workforce.

Oncoprotein HBXIP promotes tumorigenesis through MAPK/ERK pathway activation in non-small cell lung cancer

Objective:The oncoprotein,hepatitis B X-interacting protein(HBXIP),has been reported to play an important role in human malignancies.However,its functions in non-small cell lung cancer(NSCLC)are poorly understood.The goal of the present study was to identify the role of HBXIP in the regulation of NSCLC development.Methods:The level of HBXIP expression in NSCLC tissue was assessed by immunohistochemical and Western blot analyses,and its relationships with clinicopathological features and outcomes were statistically evaluated.The effects of HBXIP on NSCLC cell progression were assessed through cell viability,colony formation,and flow cytometry analyses in vitro.The mechanism by which HBXIP regulated the MAPK pathway was studied by Western blot,immunofluorescence,and immunoprecipitation assays.In addition,in vivo experiments were performed to evaluate the progression of NSCLC and ERK signaling pathway activation after HBXIP knockdown.Results:HBXIP was overexpressed in human NSCLC and was correlated with the invasiveness of NSCLC.The high expression of HBXIP in NSCLC was significantly correlated with gender(P=0.033),N stage(P=0.002),and tumor-node-metastasis stage(P=0.008).In vitro experiments using an NSCLC cell line revealed that HBXIP knockdown resulted in the suppression of cell proliferation and colony formation,which was consistent with the enhanced cell cycle arrest in G1 phase.The results of a mechanistic investigation suggested that binding of HBXIP to MEK1 protein promoted MAPK/ERK signaling pathway activation in NSCLC by preventing the proteasome-mediated degradation of MEK1.In addition,the results obtained using in vivo subcutaneous tumor xenografts confirmed that HBXIP deficiency decreased MEK1 protein levels and NSCLC tumor growth.Conclusions:Taken together,our results showed that the HBXIP-MEK interaction promoted oncogenesis via the MAPK/ERK pathway,which may serve as a novel therapeutic target for cancers in which MAPK/ERK signaling is a dominant feature.

Mechanism of Olibanum and Myrrha for the Acute Soft Tissue Injury Based on Network Pharmacology

Objective The objective of this study was to screen the therapeutic target of olibanum and myrrha on acute soft tissue injury(ASTI)by network pharmacology and to clarify their mechanisms.Methods The main chemical constituents and the targets of olibanum and myrrha were obtained by using traditional Chinese medicine systems pharmacology database and analysis platform database.The disease targets of ASTI were searched by GeneCards.The intersection targets of herbs and diseases were selected for protein interaction analysis,protein–protein interaction network was constructed,and potential protein functional modules in the network were explored.A compound–target–disease network was constructed using Cytoscape3.8.2 software.The targets were analyzed by gene ontology analysis and Kyoto Encyclopedia of Genes and Genomes enrichment analysis based on the Metascape database.Results The core active components of olibanum and myrrha were quercetin,β-sitosterol,and stigmasterol.The core targets were PGR,NCOA2,PTGS2,PRKCA,and NR3C2.Pathways in cancer,AGE-RAGE signaling pathway in diabetic complications might play a potential role in olibanum and myrrha in the treatment of ASTI.Conclusion Olibanum and myrrha have the characteristics of multiple components,multiple targets,and overall regulation in the treatment of ASTI.

Protective effect of dexmedetomidine on the propofol-induced cognitive dysfunction and neuron apoptosis in rats

Objective:To study the protective effect of dexmedetomidine on the propofol-induced cognitive dysfunction and neuron apoptosis in rats.Methods: SD rats were selected as the experimental animals and randomly divided into control group, propofol group and Dex group, propofol group and Dex group were established into propofol-induced cognitive dysfunction models by intraperitoneal injection of propofol, and Dex were given dexmedetomidine intervention on the basis of model establishment. The cognitive behavioral indicators were measured 1 d, 3 d and 5 d after model establishment;the contents of nerve marker molecules as well as inflammatory and oxidative stress response molecules in brain tissue of the hippocampus were measured at 5 d after model establishment.Results: 1 d, 3 d and 5 d after model establishment, the escape latency of propofol group were significantly longer than those of control group while the frequency of original platform crossing were significantly less than those of control group, and the escape latency of Dex group were significantly shorter than those of propofol group while the frequency of original platform crossing were significantly more than those of propofol group;5 d after model establishment, BDNF, SYN1, GPx and SOD contents in brain tissue of propofol group were significantly lower than those of control group while Nogo-A, Aβ, NF-kB p65, P2X7, TNFα, MCP1, ROS, p47PHOX and MDA contents were significantly higher than those of control group;BDNF, SYN1, GPx and SOD contents in brain tissue of Dex group were significantly higher than those of propofol group while Nogo-A, Aβ, NF-kB p65, P2X7, TNFα, MCP1, ROS, p47PHOX and MDA contents were significantly lower than those propofol group.Conclusion: Dexmedetomidine can reduce the cognitive dysfunction induced by propofol and inhibit the apoptosis induced by inflammatory response and oxidative stress response.

Effects of parecoxib sodium intervention before induction on inflammatory stress response and endocrine steady state after laparoscopic surgery

Objective:To study the effects of parecoxib sodium intervention before induction on the inflammatory stress response and endocrine steady state after laparoscopic surgery.Methods:120 cases of patients who accepted laparoscopic cholecystectomy in the Second Affiliated Hospital of Xi'an Medical University between March 2015 and December 2016 were selected and randomly divided into the parecoxib group who accepted parecoxib sodium combined with general anesthesia and the control group who accepted general anesthesia. Before anesthesia induction (T0), immediately after extubation of anesthesia (T1) and 6 hours after extubation (T2), serum levels of inflammatory cytokines and stress hormones as well as peripheral blood levels of immune cells were determined.Results:At T0, serum PGE2, TNF-α, IL-6, CRP, Cor, NE, Ins, C-P and AT-II levels as well as peripheral blood IFNγ+CD4+T cell and Perforin+CD8+T cell levels were not significantly different between two groups of patients;at T1 and T2, serum PGE2, TNF-α, IL-6, CRP, Cor, NE, Ins, C-P and AT-II levels of parecoxib group were significantly lower than those of control group while peripheral blood IFNγ+CD4+T cell and Perforin+CD8+T cell levels were significantly higher than those of control group.Conclusion: Parecoxib sodium intervention before induction can inhibit inflammatory stress response and improve endocrine steady state after laparoscopic surgery.

Effects of a Graded Brisk Walking Test with Different Intensity Levels on Elderly Patients with Essential Hypertension:A Prospective Study in Shanghai,China

Background:This study aimed to clarify the effects of a Graded Brisk Walking Test(GBWT)program on blood pressure(BP)control in elderly patients with essential hypertension and to determine its appropriate degree of intensity for elderly people.Methods:In total,421 out of 620 participants were randomly sampled from a community health center in Shanghai,China.Among them,200 and 221 patients were assigned to the intervened and control groups,respectively.They were all treated with the GBWT program for 8 weeks,16 weeks,and 24 weeks.Results:Based on ANOVA,there was no significant time main effect,condition main effect,or time by condition interaction both in heart rate and body mass index indicators(P>0.05).There were significant condition main effects,time by condition interaction,or time main effects in blood pressure(F=21.875,33.457,65.342,respectively;All P<0.05).After the intervention,significant differences in systolic blood pressure(SBP)and diastolic blood pressure(DBP)values were also observed in the two groups(P<0.05);the average values of the baseline and second phases of the intervention group were significantly lower than those of the control group(P<0.05).Significant differences in blood pressure values after the first two phases were also observed when compared with those before the intervention.Similarly,a significant difference in the first phase and second phase was also found between the BP group and intervention group.However,after the third phase(24 weeks,high-intensity exercise),no significant differences existed both in SBP and DBP groups compared with those before the intervention(P=0.07).Conclusion:GBWT is an effective exercise prescription to mitigate the essential hypertension in elderly Chinese patients,and the intensity,walking distance,and target number of steps can be adjusted according to the age of the patients.

Gene Expression Profile of Persistent Postoperative Hypertension Patients with Aldosterone-producing Adenomas

Background：Hypertension often persists after adrenalectomy for primary aldosteronism （PA）.Many studies have analyzed the outcomes of adrenalectomy for aldosterone-producing adenomas （APA） to identify predictive factors for persistent hypertension.However,differentially expressed genes in persistent postoperative hypertension remain unknown.Our aim was to describe gene expression profile of persistent postoperative hypertension patients with APA.Methods：In this study,we described and compared gene expression profiles in persistent postoperative hypertension and postoperative normotension in Chinese patients with APA using microarray analysis.Confirmation was performed with quantitative real time-polymerase chain reaction analysis.Bioinformatic analysis （gene ontology analysis,pathway analysis and network analysis） was used for further research.Results：Microarray analysis identified a total of 99 differentially expressed genes,including 18 up-regulated and 81 down-regulated genes.Among the dysregulated genes were fat atypical cadherin 1 as well as fatty acid binding protein 4 and other genes that have not been previously studied in persistent postoperative hypertension with APA.Bioinformatics analysis indicated that differentially expressed genes were associated with lipid metabolic process,metal ion binding,and cell differentiation.Pathway analysis determined that five pathways corresponded to the dysregulated transcripts.The mRNAs-ncRNAs co-expression network was composed of 49 network nodes and 72 connections between 18 coding genes and 31 noncoding genes.Conclusions：This study revealed differentially expressed genes in persistent postoperative hypertension with APA and provided a resource of candidate genes for exploration of possible drug targets and prognostic markers.

Observation on Therapeutic Effects of Acupuncture plus Psychological Intervention for Postpartum Depression

Objective： To observe the therapeutic effect of acupuncture plus psychological intervention for postpartum depression. Methods： By random number table, 85 patients with postpartum depression were divided into a treatment group and a control group. Forty-three cases in the treatment group were treated by acupuncture plus psychological intervention, once every day, five sessions per week, and rest at weekend. Forty-two cases in the control group were treated by oral administration of Fluoxetine Hydrochloride, 20 mg, once per day. The two groups were treated continuously for six weeks. The change of the score in Hamilton depression scale （HAMD） was observed and the therapeutic effect was summarized. Results： The total effective rate was 90.7% in the treatment group and 90.5% in the control group. The difference in the total effective rate between two groups was not statistically significant （P〉O.05）. In the intra-group comparisons of HAMD scores two, four and six weeks after treatment in both groups with those before treatment, the differences were statistically significant （all P〈0.05）. In comparison between the two groups at the same time point, the differences in HAMD scores were not statistically significant （all P〉0.05）. Regarding the adverse events, 5 cases had nausea, 3 cases had dizziness, and 6 cases had poor appetite in the control group; no obvious adverse events happened in the treatment group. Conclusion： Acupuncture plus psychological intervention for postpartum depression is as same as oral administration of Fluoxetine Hydrochloride in therapeutic effects, but it does not have adverse reaction.