数值模拟两相汽蚀流动的新模型和算法

提出了数值模拟两相汽蚀流动现象的新汽蚀模型和算法.提出的汽蚀模型和算法耦合了考虑紊流粘性效应的Reynolds-Averaged Navier-Stokes方程求解方法,可以自动模拟空泡起始点、空泡长度和汽蚀空泡形状.在流场计算和界面修正的迭代计算过程中,跟踪并得到液相/气相界面.数值模拟了圆锥形圆柱体和半球形圆柱体在不同汽蚀系数下的汽蚀流动现象,得到了与实验值完全吻合的数值模拟结果,并且与已发表的数值研究结果进行了比较.计算结果表明提出的汽蚀模型和算法能够有效地模拟汽蚀流动中的气泡界面和气泡长度,汽蚀模型和算法的正确性和实用性得到了相应的验证.

超高产小麦新种质HS Z6-06-56性状表现及分析

通过对普通小麦(T.aestivum)、四倍体硬粒小麦(T.durum)种间杂交超高产新种质HS Z6-06-56性状表现分析表明:该种质与受体亲本石4185相比,挑旗期、抽穗期及成熟期分别提早1,1及2 d,株高、穗长、小穗数三性状等同于受体。在产量构成三因素中,株穗数、穗粒数等同于受体,方差分析差异不显著;千粒重是构成该种质超高产的主要因素,本试验中单项指标超受体幅度达27.72%。利用种间杂种优势,是创造小麦超高产新种质的有效途径。

浙江晚稻稻种非致病细菌多样性初步研究

为了解特定生态环境中晚稻稻种的非致病细菌多样性以更有效地治理水稻主要病害 ,于 1996～ 2 0 0 1年间对采自浙江平原、丘陵及山区的 6 0 6份稻种样本进行了非致病细菌种类、频率及对水稻纹枯病和恶苗病的抑菌作用研究。从中分离出 96 0 5个菌株 ,经致病性测定、菌落形态及部分细菌学特征 (革兰氏染色、KMB培养基上的荧光色素和扩散性非荧光色素、YDC培养基上黄色菌落的产生、好气性、鞭毛及芽孢的染色镜检等 )测定后 ,选出代表菌株 6 2 2个 ,连同 80个对照菌株用Biolog及脂肪酸分析法 (FAME)进行测试。鉴定出Pseudomonas属 11个种或型及其他 14属的 2 3种非致病细菌 ,并发现Pseudomonas属中 5 5 %的种及其他属中 4 9%的种存在对水稻纹枯病或恶苗病的拮抗菌株 ,但不同种间的抑菌率存在较大差异。 3类稻区均以Bacillusspp .、Acinetobacterspp .和P .putida等3个种群为主导并同时存在许多其他相似种 ,其中

宽QRS波心动过速一例——机制分析

患者男性,55岁,既往无器质性心脏病病史,急诊行心电图示宽QRS波心动过速,通过仔细观察该心动过速自发终止时的波形,推测为室上性心动过速伴差异传导,进一步分析为左侧隐匿性旁道参与的心动过速,并经心内电生理证实。

Surrounding rock control theory and longwall mining technology innovation

To accommodate surrounding rock structure stability control problem in underground mining, we study the coupling effect principle between hydraulic support and surrounding rock, and develop a series of longwall mining technology and equipment, which solves four common technical problems that significantly undermine coal mining safety, efficiency, and high recovery and extraction rates. Based on the coupling characteristic between mining-induced stress field and supporting stress field of hydraulic support, we identify six controllable factors in the application of hydraulic support to surrounding rock, and further reveal the relationship between hydraulic support and surrounding rock in terms of the strength, the stiffness, and the stability coupling. Our findings provide a plausible solution to the longwall mining technical problem with 6-8 m mining height. By analyzing the dynamic disequilibrium characteristics between hydraulic support and surrounding rock, we propose the intelligent top coal caving control method and the high-coal-recovery-rate tech- nology for fully mechanized caving faces. With the invention of this technology, China is likely to lead the world in terms of the fully mechanized top coal caving mining technology. We are also the first to employ the intelligent coupling technology between hydraulic support and surrounding rock, and automated mining mode, and supporting system coop- erative control with automatic organization. We develop the comprehensive multi-index intelligence adjusting height decision-making mechanism and three-dimensional navigation automatic adjusting straightness technology based on shearer cutting height memory association, cutting power parameters, vibration, and video information, leading to the first set of intelligent longwall mining technology and equipment for thin seam. Our innovation makes a solid contribution to the revolution of intelligence mining technology. With the innovative use of three-dimensional coupling control principle for surrounding rock, we successfully resolve the technological difficulties of longwall mining equipment and surrounding rock control for steep dipping seam, making a breakthrough of longwall mining technology with steep dipping seam.

Spiral Steel Wire Based Fiber-Shaped Stretchable and Tailorable Triboelectric Nanogenerator for Wearable Power Source and Active Gesture Sensor

Continuous deforming always leads to the performance degradation of a flexible triboelectric nanogenerator due to the Young’s modulus mismatch of different functional layers.In this work,we fabricated a fiber-shaped stretchable and tailorable triboelectric nanogenerator(FST-TENG)based on the geometric construction of a steel wire as electrode and ingenious selection of silicone rubber as triboelectric layer.Owing to the great robustness and continuous conductivity,the FST-TENGs demonstrate high stability,stretchability,and even tailorability.For a single device with ~6 cm in length and ~3 mm in diameter,the open-circuit voltage of ~59.7 V,transferred charge of ~23.7 nC,short-circuit current of ~2.67 μA and average power of ~2.13 μW can be obtained at 2.5 Hz.By knitting several FST-TENGs to be a fabric or a bracelet,it enables to harvest human motion energy and then to drive a wearable electronic device.Finally,it can also be woven on dorsum of glove to monitor the movements of gesture,which can recognize every single finger,different bending angle,and numbers of bent finger by analyzing voltage signals.

Soil water and salt distribution under furrow irrigation of saline water with plastic mulch on ridge

Furrow irrigation when combined with plastic mulch on ridge is one of the current uppermost wa- ter-saving irrigation technologies for arid regions. The present paper studies the dynamics of soil water-salt trans- portation and its spatial distribution characteristics under irrigation with saline water in a maize field experiment. The mathematical relationships for soil salinity, irrigation amount and water salinity are also established to evaluate the contribution of the irrigation amount and the salinity of saline water to soil salt accumulation. The result showed that irrigation with water of high salinity could effectively increase soil water content, but the increment is limited com- paring with the influence from irrigation amount. The soil water content in furrows was higher than that in ridges at the same soil layers, with increments of 12.87% and 13.70% for MMF9 （the treatment with the highest water salinity and the largest amount of irrigation water） and MMF1 （the treatment with the lowest water salinity and the least amount of irrigation water） on 27 June, respectively. The increment for MMF9 was gradually reduced while that for MMF1 increased along with growth stages, the values for 17 August being 2.40% and 19.92%, respectively. Soil water content in the ridge for MMF9 reduced gradually from the surface layer to deeper layers while the surface soil water content for MMF1 was smaller than the contents below 20 cm at the early growing stage. Soil salinities for the treatments with the same amount of irrigation water but different water salinity increased with the water salinity. When water salinity was 6.04 dS/m, the less water resulted in more salt accumulation in topsoil and less in deep layers. When water salinity was 2.89 dS/m, however, the less water resulted in less salt accumulation in topsoil and salinity remained basically stable in deep layers. The salt accumulation in the ridge surface was much smaller than that in the furrow bottom under this technology, which was quite different from traditional furrow irrigation. The soil salinities for MMF7, MMF8 and MMF9 in the ridge surface were 0.191, 0.355 and 0.427 dS/m, respectively, whereas those in the furrow bottom were 0.316, 0.521 and 0.631 dS/m, respectively. The result of correlation analysis indicated that compared with irrigation amount, the irrigation water salinity was still the main factor influ- encing soil salinity in furrow irrigation with plastic mulch on ridge.

Analysis of Orange Peel Defect in St14 Steel Sheet by Electron Backscattered Diffraction (EBSD)

In this paper, the orange peel defect in the surface range of the st14 steel sheet has been investigated using the electron backscattered diffraction (EBSD) technique. It has been found that the orange peel defect in the st14 steel sheet was resulted from the local coarse grains which were produced during hot-rolling due to the critical deformation in dual-phase zone. During deep drawing, the coarse grains with {100}<001> microtexture can slip on the {112}<111> slip system to form bulging and yields orange peel defects, while the coarse grains with {112}<110> orientation do not form the defect as the Schmid factor of {112}<111> slip system in it equals zero.

Identification of squamous cell carcinoma associated proteins by proteomics and loss of beta tropomyosin expression in esophageal cancer

AIM： To assess the proteome of normal versus tumor tissue in squamous cell carcinoma of the esophagus （SCCE） in Iranian patients and compare our results with former reports by using proteomics. METHODS： Protein was extracted from normal and tumor tissues. Two dimensional electrophoresis was carried out and spots with differential expression were identified with mass spectrometry. RNA extraction and RT-PCR along with immunodetection were performed. RESULTS： Fourteen proteins were found whose expression levels differed in tumor compared to normal tissues. Mass spectrometric analysis resulted in the identification of β-tropomyosin （TMβ）, myosin light chain 2 （and its isoform）, myosin regulatory light chain 2, peroxyredoxin 2, annexin I and an unknown polypeptide as the down regulated polypeptides in tumor tissue. Heat shock protein 70 （HSP70）, TPM4-ALK fusion oncoprotein 2, myosin light polypeptide 6, keratin I, GH16431p and calreticulin were the up-regulated polypeptides found in tumor tissue. Several of these proteins, such as TMβ, HSP70, annexin Ⅰ, calreticulin, TPM4-ALK and isoforms of myosins, have been well recognized in tumorigenesis of esophageal or other types of cancers. CONCLUSION： Our study not only supports the involvement of some of the formerly reported proteins in SCCE but also introduces additional proteins found to be lost in SCCE, including TMβ.

严重感染患者血清白蛋白动力学研究(英文)

目的了解感染状态下血清白蛋白的分解和分布动力学的变化,以进一步明确感染患者低白蛋白血症的发生机制。方法对照组10例,全部为男性健康志愿者;感染组10例,均为严重感染患者,APACHEⅡ评分8～22(平均13)。采用氯胺T法,用125I 标记人体白蛋白。所有受试者一次性从上臂静脉注射20μCi的125I白蛋白,分别在第0、1、2、4、8、12、24h,第2、3、4、5、6、7、9、11、13、15、18、22、25天抽血,测定γ射线量(dpm),拟合浓度时间曲线,计算标记白蛋白的半衰期(t1/2),分布容积(Vd),中央池向周边池的转运速率(K12)。结果感染组的125I标记白蛋白t1/2(d)明显短于对照组(8.2±1.4vs.12.5±1.7,P<0.01);感染组标记白蛋白从中央池向周边池的转运速率(K12)显著高于对照组[(4.4±1.9)×10-2/hvs.(2.4±0.6)×10-2/h,P<0.05];两组分布容积(Vd)则没有显著的差异(P>0.05)。结论在感染条件下,血清白蛋白从血管内到血管外的分布速率明显增加,白蛋白的分解速率也显著增加。

Cloning,characterization of two female-specific AFLP markers and development of PCR-based sex identification method for the half-smooth tongue sole Cynoglossus semilaevis

Two female-specific AFLP(amplified fragment length polymorphism)markers(named CseF464 and CseF136)were isolated by using one selective primer combination(E-AGC/M-CTG)from the genomic DNA of 20 females and 20 males of the half-smooth tongue sole Cynoglossus semilaevis.Both the markers were re-amplified,recovered from the agarose gels,cloned and sequenced.Bioinformatics analysis indicated that the length of the two markers were 468 bp and 134 bp,respectively,and the sequences showed no similarity to each other,as well as to the known sequences deposited in the GenBank database using BLASTn.Two pairs of SCAR(sequence characterized amplified regions)primers were designed based on the sequences of the two female-specific markers.Furthermore,PCR-based genetic sex identification method was developed in Cynoglossus semilaevis.A specific fragment was amplified in all females but not in any males by using these SCAR primers on the initial 20 female and 20 male individuals of Cynoglossus semilaevis.The feasibility of the two SCAR primer pairs was confirmed in additional 100 individuals(50 females and 50 males).This allowed for reliable,rapid molecular identification of genetic sex of the species,genetic mapping on the sex chromosomes and better understanding of the sex determination and sex differentiation in the half-smooth tongue sole.

MECHANISM ON PROGRESSIVE INTRUSION OF PRESSURE WATER UNDER COAL SEAMS INTO PROTECTIVE AQUICLUDE AND ITS APPLICATION IN PREDICTION OF WATER INRUSH

This article proposes a water inrush mechanism of progressive intrusion of pressure water up into the coal floor aquiclude according to injection tests and observations. A numerical model and a criterion of water inrush are established based on the mechanism. The theory is succesSfully used in predictions of water inrush.

Tissue array for Tp53,C-myc,CCND1 gene over-expression in different tumors

AIM:To rapidly detect molecular alterations in different malignancies and investigate the possible role of Tp53,C-myc,and CCND1 genes in development of tumors in human organs and their adjacent normal tissues,as well as the possible relation between well-and poorly-differentiated tumors.METHODS:A tissue array consisting of seven different tumors was generated.The tissue array included 120 points of esophagus,120 points of stomach,80 points of rectum,60 points of thyroid gland,100 points of mammary gland,80 points of liver,and 80 points of colon.Expressions of Tp53,C-myc,and CCND1 were determined by RNA in situ hybridization.3' terminal digoxin-labeled anti-sense single stranded oligonucleotide and locked nucleic acid modifying probe were used.RESULTS:The expression level of Tp53 gene was higher in six different carcinoma tissue samples than in paracancerous tissue samples with the exception in colon carcinoma tissue samples(P < 0.05).The expression level of CCND1 gene was significantly different in different carcinoma tissue samples with the exception in esophagus and colon carcinoma tissue samples.The expression level of C-myc gene was different in esophagus carcinoma tissue samples(c2 = 18.495,P = 0.000),stomach carcinoma tissue samples(c2 = 23.750,P = 0.000),and thyroid gland tissue samples(c2 = 10.999,P = 0.004).The intensity of signals was also different in different carcinoma tissue samples and paracancerous tissue samples.CONCLUSION:Over-expression of the Tp53,CCND1,and C-myc genes appears to play a role in development of human cancer by regulating the expression of mRNA.Tp53,CCND1 and C-myc genes are significantly correlated with the development of different carcinomas.

HIGH-ACCURACY SYNCHRONIZA-TION CONTROL WITH HYBRID NEURAL NETWORKS

A novel nonlinear control algorithm based on hybrid neural networks ispresented to cope with the high-accuracy synchronization control problem for a dual-actuatorelectrohydraulic drive system which plays an important role for the development of elastomericlaunchers. A new objective function for better synchronization performance is introduced and alearning algorithm to adjust the weights of the neural network, based on the gradient descentalgorithm, is also derived. The hybrid neural network control algorithm guarantees high-accuracysynchronization performance of two motion cylinders and fast dynamic response as well as goodstability of the control system. Prototype test results on the dual-actuator electrohydraulic drivesystem verifys the effectiveness of the proposed approach.

Adiponectin deficiency enhances colorectal carcinogenesis and liver tumor formation induced by azoxymethane in mice

AIM:To investigate the causal relationship between hypoadiponectinemia and colorectal carcinogenesis in in vivo experimental model,and to determine the con-tribution of adiponectin defi ciency to colorectal cancer development and proliferation.METHODS:We examined the influence of adiponectin defi ciency on colorectal carcinogenesis induced by the administration of azoxymethane(AOM)(7.5 mg/kg,in-traperitoneal injection once a week for 8 wk),by using adiponectin-knockout(KO) mice.RESULTS:At 53 wk after the fi rst AOM treatment,KOmice developed larger and histologically more progres-sive colorectal tumors with greater frequency com-pared with wild-type(WT) mice,although the tumor incidence was not different between WT and KO mice.KO mice showed increased cell proliferation of colorec-tal tumor cells,which correlated with the expression levels of cyclooxygenase-2(COX-2) in the colorectal tumors.In addition,KO mice showed higher incidence and frequency of liver tumors after AOM treatment.Thirteen percent of WT mice developed liver tumors,and these WT mice had only a single tumor.In contrast,50% of KO mice developed liver tumors,and 58% of these KO mice had multiple tumors.CONCLUSION:Adiponectin deficiency enhances colorectal carcinogenesis and liver tumor formation induced by AOM in mice.This study strongly suggests that hypoadiponectinemia could be involved in the pathogenesis for colorectal cancer and liver tumor in human subjects.

The impact of graphene on neural regenerative medicine

There is increasing interest in studying carbon-based nanomaterials（CBNs）for use in regenerative medicine.Some carbon crystalline structures,such as graphene,carbon nanotubes/nanofibers,boron nitride nanosheets/nanotubes and fullerenes,as well as disordered structures,such as diamond-like carbon,glasslike carbon,and amorphous carbon,are now being considered as promising scaffolds（Ferreira et al.,2015;Kabiri et al.,2015）,

Analysis of SSRs Derived from an EST Library of Half-Smooth Tongue Sole Cynoglossus semilaevis

A complementary DNA(cDNA) library was constructed from half-smooth tongue sole spleen. A long-read expressed sequence tag(EST) database was generated,containing 3100 cDNA clones,of which 220 clones were fully sequenced. A total of 1060 non-redundant simple sequence repeats(SSRs) were obtained from the cDNA library. An average of 5 kb sequence generates 1 SSR in the half-smooth tongue sole spleen cDNA library. The proportion of the SSR unit size was different in the cDNA library. The monomeric repeats(51.4%) are the most abundant class of SSR in the dataset. The dimeric,trimeric,tetrameric and hexameric re-peats are represented in decreasing proportions of 27.2%,16.0%,2.8% and 1.9%,respectively. The frequency of pentameric repeats was observed the least(only 0.7%) . Most of the monomeric and dimeric repeats are distributed in 3' and 5' un-translation region. If translation regions are considered merely,trimeric repeats are the highest,accounting for 57% of the total microsatellites.

黄山短尾猴的左右手倾向(英文)

就取食过程中惯用左手还是右手的问题,我们对安徽省黄山的一群短尾猴进行了研究。结果发现在调查的33只个体中,9只惯用右手取食,11只惯用左手取食,另外13只对左右手的使用没有偏好。而且短尾猴在左右手的偏好方面也没有性别或年龄的差异。以往的一些研究表明猕猴类动物习惯于用左手取食,但是研究群的短尾猴并没有表现出这种倾向。我们将短尾猴的研究结果与猕猴属其它种类进行了比较,并讨论了猕猴属在这一行为上的多样性。

Rapid Detection Co-infections of Classical Swine Fever Virus and Porcine Reproductive and Respiratory Syndrome Virus by One-step Multiplex RT-PCR

Classical swine fever virus （CSFV） and porcine reproductive and respiratory syndrome virus （PRRSV） have caused immense economic loss in the pig industry and are considered to be the two most important infectious diseases of pigs in the world A multiplex reverse transcription polymerase chain reaction （multiplex RT-PCR） was developed for CSFV and PRRSV co-infections or infections, respectively. A set of two pairs of primer was designed based on the sequence of nonstructural protein NS54B of CSFV and ORF7 gene of PRRSV. The diagnostic accuracy of multiplex RT-PCR assay was evaluated by using 56 field clinical samples by multiplex RT-PCR, single RT-PCR and sequence analysis; and the specificity of multiplex PCR was verified by using constructed plasmids containing the specific viral target fragments of PRRSV and CSFV, respectively. The results indicated that this assay could reliably differentiate PRRSV and CSFV in co-infection samples. The multiplex RT-PCR developed in this study might provide a new avenue to the rapid the detection of CSFV and PRRSV in one reaction.