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Differential expression of miRNAs and mRNAs in the livers of largemouth bass Micropterus salmoides under heat stress
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作者 Xuqian ZHAO Zijie LIN +4 位作者 Caijuan LI Hao ZHU Lingling LI Wenjia MAO Qufei LING 《Journal of Oceanology and Limnology》 SCIE CAS CSCD 2024年第2期594-608,共15页
Global warming threatens freshwater ecosystems and compromises fish survival.To elucidate the role of miRNAs in the livers of heat stressed largemouth bass,juvenile fish was subject to heat stress under 37°C.Both... Global warming threatens freshwater ecosystems and compromises fish survival.To elucidate the role of miRNAs in the livers of heat stressed largemouth bass,juvenile fish was subject to heat stress under 37°C.Both mRNA-seq and miRNA-seq were conducted on the liver tissues under control and heat stress conditions.Differential gene expression analysis and enrichment analysis were performed on mRNA and miRNA expression profiles.A total of 406 differentially expressed genes(DEGs)were discovered,of which 212 were up-regulated and 194 were down-regulated.Most of the DEGs were significantly implicated in the regulation of“protein processing in endoplasmic reticulum”,“proteasome”,“steroid biosynthesis”,and“ornithine decarboxylase inhibitor activity”pathways.In addition,47 differentially expressed miRNAs(DEMs)were identified in largemouth bass livers under heat stress,including 21 up-regulated and 25 down-regulated miRNAs.A negatively regulated miRNA-mRNA network including 12 miRNAs and 19 mRNAs was constructed with DEMs involved in“protein degradation”,“calcium ion regulation”,“cell apoptosis”,and“lipid metabolism”.Moreover,this study indicated novel-miR-144 activated the IRE1 signaling pathway by targeting txndc5 to induce liver apoptosis in largemouth bass under heat stress.This study revealed the involvement of miRNA regulation in largemouth bass in response to heat stress. 展开更多
关键词 heat stress largemouth bass miRNA-mRNA interaction
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Biocatalytic enhancement of laccase immobilized on ZnFe_(2)O_(4) nanoparticles and its application for degradation of textile dyes
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作者 Yuhang Wei Qingpeng Zhu +3 位作者 Weiwei Xie Xinyue Wang Song Li Zhiming Chen 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2024年第4期216-223,共8页
Efficient and convenient treatment of industrial dyeing wastewater is of great significance to guarantee human and animal health.This work presented the enhanced catalytic activity at pH 3.0 of laccase immobilized on ... Efficient and convenient treatment of industrial dyeing wastewater is of great significance to guarantee human and animal health.This work presented the enhanced catalytic activity at pH 3.0 of laccase immobilized on amino-functionalized ZnFe_(2)O_(4) nanoparticles(ZnFe_(2)O_(4)-laccase)and its application for the degradation of textile dyes.Due to the existence of a large number of oxygen vacancies on the surface of the ZnFe_(2)O_(4) nanoparticles,negative ions accumulated on the magnetic carriers,which resulted in a harsh optimal pH value of the ZnFe_(2)O_(4)-laccase.Laccase activity assays revealed that the ZnFe_(2)O_(4)-laccase possessed superior pH and thermal stabilities,excellent reusability,and noticeable organic solvent tolerance.Meanwhile,the ZnFe_(2)O_(4) laccase presented efficient and sustainable degradation of high concentrations of textile dyes.The initial decoloration efficiencies of malachite green(MG),brilliant green(BG),azophloxine,crystal violet(CV),reactive blue 19(RB19),and procion red MX-5B were approximately 99.1%,95.0%,93.3%,87.4%,86.1%,and 85.3%,respectively.After 10 consecutive reuses,the degradation rates of the textile dyes still maintained about 98.2%,92.5%,83.2%,81.5%,79.8%and 65.9%,respectively.The excellent dye degradation properties indicate that the ZnFe_(2)O_(4)-laccase has a technical application in high concentrations of dyestuff treatment. 展开更多
关键词 ZnFe_(2)O_(4)-laccase Catalytic activity Stability and reusability Degradation of textile dye
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Enhanced axonal regeneration and functional recovery of the injured sciatic nerve in a rat model by lithium-loaded electrospun nanofibrous scaffolds
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作者 Banafsheh Dolatyar Bahman Zeynali +2 位作者 Iman Shabani Azita Parvaneh Tafreshi Reza Karimi-Soflou 《Bio-Design and Manufacturing》 SCIE EI CAS CSCD 2024年第5期701-720,共20页
Increasing evidence indicates that engineered nerve grafts have great potential for the regeneration of peripheral nerve injuries(PNIs).While most studies have focused only on the topographical features of the grafts,... Increasing evidence indicates that engineered nerve grafts have great potential for the regeneration of peripheral nerve injuries(PNIs).While most studies have focused only on the topographical features of the grafts,we have considered both the biophysical and biochemical manipulations in our applied nanoscaffold.To achieve this,we fabricated an electrospun nanofibrous scaffold(ENS)containing polylactide nanofibers loaded with lithium(Li)ions,a Wnt/β-catenin signaling activator.In addition,we seeded human adipose-derived mesenchymal stem cells(hADMSCs)onto this engineered scaffold to examine if their differentiation toward Schwann-like cells was induced.We further examined the efficacy of the scaffolds for nerve regeneration in vivo via grafting in a PNI rat model.Our results showed that Li-loaded ENSs gradually released Li within 11 d,at concentrations ranging from 0.02 to(3.64±0.10)mmol/L,and upregulated the expression of Wnt/β-catenin target genes(cyclinD1 and c-Myc)as well as those of Schwann cell markers(growth-associated protein 43(GAP43),S100 calcium binding protein B(S100B),glial fibrillary acidic protein(GFAP),and SRY-box transcription factor 10(SOX10))in differentiated hADMSCs.In the PNI rat model,implantation of Li-loaded ENSs with/without cells improved behavioral features such as sensory and motor functions as well as the electrophysiological characteristics of the injured nerve.This improved function was further validated by histological analysis of sciatic nerves grafted with Li-loaded ENSs,which showed no fibrous connective tissue but enhanced organized myelinated axons.The potential of Li-loaded ENSs in promoting Schwann cell differentiation of hADMSCs and axonal regeneration of injured sciatic nerves suggests their potential for application in peripheral nerve tissue engineering. 展开更多
关键词 Stem cell Schwann cell differentiation Electrospun nanofibrous scaffold Lithium ion Nerve regeneration
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Characterization of the infectivity of an Indonesian Zika virus strain in mammalian cell lines
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作者 Justus Thomas Obiajulu Sievers Anom Bowolaksono R.Tedjo Sasmono 《Asian Pacific Journal of Tropical Biomedicine》 SCIE CAS 2024年第5期215-224,共10页
Objective:To characterize the infection patterns and growth characteristics of the Zika virus(ZIKV)strain JMB-185 from Indonesia in various mammalian cell lines.Methods:ZIKV was grown in human(A549,HEK293,HepG2,Huh7,J... Objective:To characterize the infection patterns and growth characteristics of the Zika virus(ZIKV)strain JMB-185 from Indonesia in various mammalian cell lines.Methods:ZIKV was grown in human(A549,HEK293,HepG2,Huh7,Jurkat,and THP-1)and non-human mammalian(RAW264.7,Vero,and Vero76)cell lines.Viral replication kinetics were measured using plaque assay,while intra-and extracellular viral RNA concentrations were assessed using RT-PCR.Flow cytometry was used to quantify the infected cells and cell viability was measured using an MTT assay.The ability of ZIKV to infect cell lines was visualized using a fluorescence immunostaining assay.Results:This ZIKV strain preferentially infected the lung,kidney,and liver cell lines A549,HEK293,Huh7,Vero,and Vero76,but not the immune cells Jurkat,RAW264.7,and THP-1.By contrast,the ZIKV showed no sign of infection in HepG2 cells,while maintaining viral titer over 3 days post-infection,with no infection recorded in immunostaining,no increase in viral RNA,and no indication of cell deterioration.Conclusions:The Indonesian ZIKV strain has a similar infection profile as other strains,except for its poor infectivity on HepG2 cells.Information on the growth characteristics of Indonesia ZIKV will help expand our understanding of the biology of ZIKV which will be useful for various applications including antiviral discovery. 展开更多
关键词 Zika virus Infectivity REPLICATION Cell lines In vitro
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Identification of Key Blood Biomarkers Linking Di(2-ethylhexyl)Phthalate and Autoimmune Diseases in Adolescents Mice
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作者 XU Xiao Mei LI Xiao Lu +8 位作者 HU Die CAI Xiao Yue LI Qi Meng ZHENG Yuan Zhuo CAI Yang QI Yin Yin XU Tao ZHAO Ling Li NING Xia 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 2024年第5期521-525,共5页
Di(2-ethylhexyl)phthalate(DEHP)is a widely used plasticizer known for its reproductive developmental and immune system toxicity,mainly through esophagal,dermal,and respiratory exposure^([1-3]).Maternal exposure to DEH... Di(2-ethylhexyl)phthalate(DEHP)is a widely used plasticizer known for its reproductive developmental and immune system toxicity,mainly through esophagal,dermal,and respiratory exposure^([1-3]).Maternal exposure to DEHP during pregnancy can lead to adverse birth outcomes in offspring,including impacts on the thyroid system of adolescent offspring^([2-4]). 展开更多
关键词 BLOOD RESPIRATORY PREGNANCY
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Physiological Mechanism of Exogenous Selenium in Alleviating Mercury Stress on Pakchoi(Brassica campestris L.)
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作者 Chengxu Qian Qiangwen Chen +4 位作者 Leiyu Jiang Xiaoyan Yang Shen Rao Weiwei Zhang Feng Xu 《Phyton-International Journal of Experimental Botany》 SCIE 2024年第5期951-962,共12页
The objective of this study was to explain the physiological mechanisms through which Na_(2)SeO_(3) mitigates the growth and developmental inhibition of pakchoi under HgCl_(2)stress.The results showed that treatment w... The objective of this study was to explain the physiological mechanisms through which Na_(2)SeO_(3) mitigates the growth and developmental inhibition of pakchoi under HgCl_(2)stress.The results showed that treatment with HgCl_(2)(40 mg L^(−1))led to reduced biomass,dwarfing,root shortening,and root tip necrosis in pakchoi.Compared to control(CK),the activities of superoxide dismutase(SOD)and peroxidase(POD)in Hg treatment increased,and the content of malondialdehyde(MDA)also dramatically increased,which negatively impacted the growth of pakchoi.Low concentrations of Na_(2)SeO_(3)(0.2 mg L^(−1))significantly increased the content of soluble sugars compared with control,while chlorophyll,soluble proteins,free amino acids,and vitamin C had no significant changes.The results of the mixed treatments with HgCl_(2)and Na_(2)SeO_(3) suggested that selenium may be able to reduce the toxicity of mercury in pakchoi.The biomass,plant height,root length,chlorophyll content,soluble protein,other physiological indicators,and proline showed significant increases compared with the HgCl_(2)treatment.Additionally,the MDA content and mercury accumulation in pakchoi decreased.Our results revealed the antagonistic effects of selenium and mercury in pakchoi.Thus,a theoretical basis for studying pakchoi’s mercuryexcreted and selenium-rich cultivation technology was provided. 展开更多
关键词 SELENIUM mercury stress PAKCHOI physiological properties antioxidant enzymes
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Effects of vitamin family members on insulin resistance and diabetes complications
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作者 Hong-Jin Chen Min Wang +2 位作者 Ding-Min Zou Gui-You Liang Si-Yuan Yang 《World Journal of Diabetes》 SCIE 2024年第3期568-571,共4页
The following letter to the editor highlights the article“Effects of vitamin D supplementation on glucose and lipid metabolism in patients with type 2 diabetes mellitus and risk factors for insulin resistance”in Wor... The following letter to the editor highlights the article“Effects of vitamin D supplementation on glucose and lipid metabolism in patients with type 2 diabetes mellitus and risk factors for insulin resistance”in World J Diabetes 2023 Oct 15;14(10):1514-1523.It is necessary to explore the role of vitamin family members in insulin resistance and diabetes complications. 展开更多
关键词 VITAMIN Insulin resistance Diabetes complications LETTER
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Foodborne toxin Aflatoxin B_(1)induced glomerular podocyte inflammation through proteolysis of RelA,downregulation of miR-9 and CXCR4/TXNIP/NLRP3 pathway
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作者 Jie Zhang Shuang Yang +7 位作者 Baocai Xu Zihui Qin Xinyi Guo Ben Wei Qinghua Wu Kamil Kuca Tushuai Li Wenda Wu 《Food Science and Human Wellness》 SCIE CAS CSCD 2024年第4期2289-2309,共21页
Aflatoxin B_(1)(AFB_(1))is a naturally-occurring mycotoxin and recognized as the most toxic foodborne toxin,particularly causing damages to kidney.Glomerular podocytes are terminally differentiated epithelial cells.AF... Aflatoxin B_(1)(AFB_(1))is a naturally-occurring mycotoxin and recognized as the most toxic foodborne toxin,particularly causing damages to kidney.Glomerular podocytes are terminally differentiated epithelial cells.AFB_(1)induces podocyte inflammation,proteinuria and renal dysfunction.Studying the mechanism of AFB_(1)-induced podocyte inflammation and murine kidney dysfunction,we detected that AFB_(1)increased ubiquitindependent degradation of the transcription factor RelA through enhanced interaction of RelA with E3 ubiquitin ligase tripartite motif containing 7(TRIM7)in mouse podocyte clone-5(MPC-5)and mouse glomeruli.Reduction of RelA resulted in decreasing microRNA-9(miR-9)and activating the chemokine receptor 4(CXCR4),thioredoxin interacting protein(TXNIP),and NOD-like receptor pyrin domain-containing 3(NLRP3)signaling axis(CXCR4/TXNIP/NLRP3 pathway),leading to podocyte inflammation.We also determined that downregulation of miR-9 led to CXCR4 expression and the downstream TXNIP/NLRP3 pathway activation.Overexpression of miR-9 or deletion of CXCR4 suppressed AFB_(1)-induced CXCR4/TXNIP/NLRP3 pathway,resulting in alleviating podocyte inflammation and kidney dysfunction.Our findings indicated that ubiquitin-dependent proteolysis of RelA,downregulation of miR-9,and activation of CXCR4/TXNIP/NLRP3 pathway played an essential role in AFB_(1)-induced glomerular podocyte inflammation.Our study revealed a novel mechanism,via RelA,for the control of AFB_(1)’s nephrotoxicity,leading to an effective protection of food safety and public health. 展开更多
关键词 Aflatoxin B_(1) Podocyte inflammation miRNA-9 Chemokine(C-X-C motif)receptor 4 RelA ubiquitin-dependent degradation
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The Influence of Aerial Exposure on Sea Anemones Aulactinia veratra Mucin Genes Expression Using the RNA Sequencing
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作者 Alaa Haridi 《Open Journal of Marine Science》 2024年第1期1-18,共18页
Mucin genes are the main component of mucus. The sea anemone species, Aulactinia veratra (Phylum Cnidaria) contains different types of mucin genes. In the intertidal zone, A. veratra is found to be exposed to air duri... Mucin genes are the main component of mucus. The sea anemone species, Aulactinia veratra (Phylum Cnidaria) contains different types of mucin genes. In the intertidal zone, A. veratra is found to be exposed to air during the low tide and produces large quantities of mucus as an external covering. The relation between low tide and mucus secretion is still unclear, and what is the role of mucin during arial exposure is not yet investigated. This study hypothesised that the mucin genes in A. veratra would have significantly high expression in response to aerial exposure. Therefore, the aim of current study was to examine and analyses the response of A. veratra mucins in response to an experiment involving three hours of aerial exposure. To achieve this, aim the RNA-sequencing and bioinformatics analyses were used to examine the expression profile of A. veratra mucin genes in response to aerial exposure. The generated results have shown that, Mucin4-like and mucin5B-like were up-regulated in response to the three hours of aerial exposure in A. veratra. This finding shows a significant role of mucin5B-like and mucin4-like genes in response to air stress at low tide. The data generated from this study could be used in conjunction with future mucin gene studies of sea anemones and other cnidarians to compare A. veratra mucin gene expression results across time, and to extend our understanding of mucin stress response in this phylum. 展开更多
关键词 Aulactinia veratra Mucin4-Like Mucin5B-Like CNIDARIA Mucin Gene Expression RNA-Sequencing Sea Anemones MUCUS Aerial Exposure
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Hydraulic properties and drought response of a tropical bamboo (Cephalostachyum pergracile)
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作者 Wanwalee Kongjarat Lu Han +10 位作者 Amy Ny Aina Aritsara Shu-Bin Zhang Gao-Juan Zhao Yong-Jiang Zhang Phisamai Maenpuen Ying-Mei Li Yi-Ke Zou Ming-Yi Li Xue-Nan Li Lian-Bin Tao Ya-Jun Chen 《Plant Diversity》 SCIE CAS CSCD 2024年第3期406-415,共10页
Bamboo plants are an essential component of tropical ecosystems,yet their vulnerability to climate extremes,such as drought,is poorly understood due to limited knowledge of their hydraulic properties.Cephalostachyum p... Bamboo plants are an essential component of tropical ecosystems,yet their vulnerability to climate extremes,such as drought,is poorly understood due to limited knowledge of their hydraulic properties.Cephalostachyum pergracile,a commonly used tropical bamboo species,exhibited a substantially higher mortality rate than other co-occurring bamboos during a severe drought event in 2019,but the underlying mechanisms remain unclear.This study investigated the leaf and stem hydraulic traits related to drought responses,including leaf-stem embolism resistance(P50leaf;P50stem) estimated using optical and X-ray microtomography methods,leaf pressure-volume and water-releasing curves.Additionally,we investigated the seasonal water potentials,native embolism level(PLC) and xylem water source using stable isotope.We found that C.pergracile exhibited strong resistance to embolism,showing low P50leaf,P50stem,and turgor loss point,despite its rapid leaf water loss.Interestingly,its leaves displayed greater resistance to embolism than its stem,suggesting a lack of effective hydraulic vulnerability segmentation(HVS) to protect the stem from excessive xylem tension.During the dry season,approximately 49% of the water was absorbed from the upper 20-cm-deep soil layer.Consequently,significant diurnal variation in leaf water potentials and an increase in midday PLC from 5.87±2.33% in the wet season to 12.87±4.09%in the dry season were observed.In summary,this study demonstrated that the rapid leaf water loss,high reliance on surface water,and a lack of effective HVS in C.pergracile accelerated water depletion and increased xylem embolism even in the typical dry season,which may explain its high mortality rate during extreme drought events in 2019. 展开更多
关键词 Climate change DROUGHT Hydraulic safety Hydraulic vulnerability segmentation Stable isotope Tree mortality
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Epidemiological observations of invasive group B Streptococcus infections in six major hospitals in Peninsular Malaysia
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作者 AbdulRahman Muthanna Mohd Nasir Mohd Desa +10 位作者 Nurul Asyikin Abdul Rahman Nurul Diana Dzaraly Nurul Hana Zainal Baharin Nur Afiza Aziz Chua Hui Shan Zalina Ismail Lailatul Akmar Mat Nor Marlindawati Mohd Ali Nur Hanani Ahmad Mohammad Noor Amal Azmai Syafinaz Amin-Nordin 《Asian Pacific Journal of Tropical Medicine》 SCIE CAS 2024年第9期384-391,共8页
Objective:To address the lack of research on invasive group B Streptococcus(GBS)infections in Malaysia and Southeast Asia through a comprehensive analysis of GBS isolates obtained from hospitals.Methods:Medical record... Objective:To address the lack of research on invasive group B Streptococcus(GBS)infections in Malaysia and Southeast Asia through a comprehensive analysis of GBS isolates obtained from hospitals.Methods:Medical records from patients with GBS infection isolated from the sterile site,such as blood and cerebrospinal fluid from 14 July 2019 to 15 December 2020,were reviewed from six major hospitals in Peninsular Malaysia.Inclusion criteria were invasive GBS,sterile sites and non-repeated GBS isolated from the same patients in the same admission.Viable isolates were re-identified for GBS and serotyped.Results:A total of 118 patients were eligible,with a majority of non-pregnant adults(76.3%).Over half of the patients(62.7%)had underlying medical conditions,with diabetes as the most common disease,followed by respiratory disease,renal disease,cardiovascular disease and skin and soft tissue disease.The most common manifestations were sepsis,followed by soft tissue abscess,diabetic foot ulcer,wet gangrene and cellulitis.The overall mortality was 7.6%.The most common serotype was serotype桋.Conclusions:Invasive GBS infection among non-pregnant adults showed a rising trend,particularly among diabetic individuals.The study underscores the importance of reducing risk factors and highlights the necessity of developing GBS vaccination as a preventive strategy for both infants and adults. 展开更多
关键词 Streptococcus agalactiae Group B Streptococcus Invasive GBS infections MALAYSIA
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Molecular Phylogeny, Species Delimitation and Taxonomic Revision of the Australian Spider Wasp Genus Heterodontonyx Haupt, 1935 (Hymenoptera: Pompilidae)
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作者 Mahin Sadat Chavoshi Jolfaei Juanita Rodriguez 《Advances in Entomology》 2024年第2期105-128,共24页
Heterodontonyx (Pompilidae: Pepsinae) is an understudied genus with Australasiandistribution and most species are endemic to Australia. There have recently been sometaxonomic changes involving the genera Heterodontony... Heterodontonyx (Pompilidae: Pepsinae) is an understudied genus with Australasiandistribution and most species are endemic to Australia. There have recently been sometaxonomic changes involving the genera Heterodontonyx and Cryptocheilus based on molecular and morphological evidence, however, phylogenetic relationships within Heterodontonyx, species delimitation and formal revision have not been conducted. Here, we construct a Maximum Likelihood phylogeny estimate of Heterodontonyx based on ITS1, 5.8S, ITS2 region of 30 samples representing seven Heterodontonyx morphospecies. bPTP species delimitation approach was employed and ultimately the taxonomic revision of the genus was carried out. Phylogenetic analyses strongly support the monophyly of Heterodontonyx and the species delimitation method recognizes eight species among which one new species is identified. 展开更多
关键词 Pompilidae Taxonomy Australian Pomplids PHYLOGENETICS
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Caloric restriction-mimetics for the reduction of heart failure risk in aging heart: with consideration of gender-related differences 被引量:3
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作者 Lei Pang Xi Jiang +6 位作者 Xin Lian Jie Chen Er-Fei Song Lei-Gang Jin Zheng-Yuan Xia Hai-Chun Ma Yin Cai 《Military Medical Research》 SCIE CAS CSCD 2023年第1期113-133,共21页
The literature is full of claims regarding the consumption of polyphenol or polyamine-rich foods that offer some protection from developing cardiovascular disease(CVD). This is achieved by preventing cardiac hypertrop... The literature is full of claims regarding the consumption of polyphenol or polyamine-rich foods that offer some protection from developing cardiovascular disease(CVD). This is achieved by preventing cardiac hypertrophy and protecting blood vessels through improving the function of endothelium. However, do these interventions work in the aged human hearts? Cardiac aging is accompanied by an increase in left ventricular hypertrophy, along with diastolic and systolic dysfunction. It also confers significant cardiovascular risks for both sexes. The incidence and prevalence of CVD increase sharply at an earlier age in men than women. Furthermore, the patterns of heart failure differ between sexes, as do the lifetime risk factors. Do caloric restriction(CR)-mimetics, rich in polyphenol or polyamine, delay or reverse cardiac aging equally in both men and women? This review will discuss three areas:(1) mechanisms underlying age-related cardiac remodeling;(2) gender-related differences and potential mechanisms underlying diminished cardiac response in older men and women;(3) we select a few polyphenol or polyamine rich compounds as the CRmimetics, such as resveratrol, quercetin, curcumin, epigallocatechin gallate and spermidine, due to their capability to extend health-span and induce autophagy. We outline their abilities and issues on retarding aging in animal hearts and preventing CVD in humans. We discuss the confounding factors that should be considered for developing therapeutic strategies against cardiac aging in humans. 展开更多
关键词 Cardiovascular disease Cardiac aging Caloric restriction Gender difference Caloric restriction-mimetics Dietary compounds Clinical application
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Reproductive Biology and Herkogamy of <i>Psychotria elata</i>(Rubiaceae), a Distylous Species of the Tropical Rain Forests of Costa Rica
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作者 Celice Alexandre Silva Jorge Arturo Lobo Segura 《American Journal of Plant Sciences》 2015年第3期433-444,共12页
The floral morphology, breeding system and pollinators of four natural populations of Psychotria elata (Rubiaceae), found in tropical rainforests of Costa Rica, were examined. Anisoplethy and distylous morphology were... The floral morphology, breeding system and pollinators of four natural populations of Psychotria elata (Rubiaceae), found in tropical rainforests of Costa Rica, were examined. Anisoplethy and distylous morphology were observed in all studied populations. The number of flowers per inflorescence was significantly higher in the most abundant morph (p 0.05). Reciprocal herkogamy was detected in only one of the morphs in one population. Breakdown of the heteromorphic incompatibility system was observed in the populations Tirimbina and Rara Avis. Pollen production was significantly lower in thrum morphs of Tirimbina. Seedless fruits were formed in two of the four populations. One-seeded fruits were formed in all populations and were predominant in thrum morphs of Tirimbina (9.15%) and Zurqui (75%). According to our visitation records, hummingbirds, butterflies and moths are the main pollinators of P. elata flowers. 展开更多
关键词 Disassortative Mating Floral Morphology Geographic Variation Morph Ratio POLLINATORS
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Per1/Per2 double knockout transcriptome analysis reveals circadian regulation of hepatic lipid metabolism
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作者 Yiran Bu Si Chen +6 位作者 Mengcheng Ruan Libang Wu Hualin Wang Na Li Xiuju Zhao Xiaoli Yu Zhiguo Liu 《Food Science and Human Wellness》 SCIE CSCD 2023年第5期1716-1729,共14页
Scope: Circadian disorder and high-fat diet(HFD)can disturb lipid metabolism homeostasis and may promote the development of various metabolic diseases. The relationship between them is of great concern. This study aim... Scope: Circadian disorder and high-fat diet(HFD)can disturb lipid metabolism homeostasis and may promote the development of various metabolic diseases. The relationship between them is of great concern. This study aimed to explore the effects of Per1/Per2 double knockout(DKO)on hepatic lipid metabolism in mice under HFD and HFD with docosahexaenoic acid(DHA)substitution. Methods and results: Both wild type(WT)and DKO male C57BL/6 mice were fed with normal chow diet(CON), HFD, or HFD with DHA substitution(AO)for 15 weeks. At the end of the experiment, mice were sacrificed at zeitgeber time(ZT)0(7:00 am)or ZT12(7:00 pm). Pathological indicators were determined using histological and biochemical methods. Hepatic transcriptome sequencing analysis showed that DKO mice exhibited multiple dysfunctions in diurnal rhythm, drug metabolism, cell cycle, cancer pathways, and lipid metabolism. HFD had greater effects on fatty acid oxidation and cholesterol synthesis and metabolism in Per1-/-Per2-/-mice, which was improved by DHA substitution. Conclusions: Per1/Per2 played an important role in the circadian regulation of hepatic lipid metabolism, and DKO mice were more sensitive to HFD. DHA can improve circadian-related lipid metabolism disruption induced by HFD in mice. 展开更多
关键词 DHA High-fat diet Lipid metabolism Per1/Per2 TRANSCRIPTOMICS
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Cardiopulmonary prognosis of prophylactic endotracheal intubation in patients with upper gastrointestinal bleeding undergoing endoscopy
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作者 Yufang Lin Fei’er Song +9 位作者 Weiyue Zeng Yichi Han Xiujuan Chen Xuanhui Chen Yu Ouyang Xueke Zhou Guoxiang Zou Ruirui Wang Huixian Li Xin Li 《World Journal of Emergency Medicine》 SCIE CAS CSCD 2023年第5期372-379,共8页
BACKGROUND:It is controversial whether prophylactic endotracheal intubation(PEI)protects the airway before endoscopy in critically ill patients with upper gastrointestinal bleeding(UGIB).The study aimed to explore the... BACKGROUND:It is controversial whether prophylactic endotracheal intubation(PEI)protects the airway before endoscopy in critically ill patients with upper gastrointestinal bleeding(UGIB).The study aimed to explore the predictive value of PEI for cardiopulmonary outcomes and identify high-risk patients with UGIB undergoing endoscopy.METHODS:Patients undergoing endoscopy for UGIB were retrospectively enrolled in the eICU Collaborative Research Database(eICU-CRD).The composite cardiopulmonary outcomes included aspiration,pneumonia,pulmonary edema,shock or hypotension,cardiac arrest,myocardial infarction,and arrhythmia.The incidence of cardiopulmonary outcomes within 48 h after endoscopy was compared between the PEI and non-PEI groups.Logistic regression analyses and propensity score matching analyses were performed to estimate effects of PEI on cardiopulmonary outcomes.Moreover,restricted cubic spline plots were used to assess for any threshold effects in the association between baseline variables and risk of cardiopulmonary outcomes(yes/no)in the PEI group.RESULTS:A total of 946 patients were divided into the PEI group(108/946,11.4%)and the non-PEI group(838/946,88.6%).After propensity score matching,the PEI group(n=50)had a higher incidence of cardiopulmonary outcomes(58.0%vs.30.3%,P=0.001).PEI was a risk factor for cardiopulmonary outcomes after adjusting for confounders(odds ratio[OR]3.176,95%confidence interval[95%CI]1.567-6.438,P=0.001).The subgroup analysis indicated the similar results.A shock index>0.77 was a predictor for cardiopulmonary outcomes in patients undergoing PEI(P=0.015).The probability of cardiopulmonary outcomes in the PEI group depended on the Charlson Comorbidity Index(OR 1.465,95%CI 1.079-1.989,P=0.014)and shock index>0.77(compared with shock index≤0.77[OR 2.981,95%CI 1.186-7.492,P=0.020,AUC=0.764]).CONCLUSION:PEI may be associated with cardiopulmonary outcomes in elderly and critically ill patients with UGIB undergoing endoscopy.Furthermore,a shock index greater than 0.77 could be used as a predictor of a worse prognosis in patients undergoing PEI. 展开更多
关键词 Prophylactic endotracheal intubation Upper gastrointestinal bleeding Cardiopulmonary outcomes eICU Collaborative Research Database
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Analysis on Antibacterial, Detumescent and Antioxidant Activity of Huoshan Large-leaf Yellow Tea
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作者 Jinwu CHEN Wen HU +4 位作者 Yimeng LI Jiaojiao WANG Yafei LIU Yi LI Lulu QI 《Medicinal Plant》 CAS 2023年第4期1-5,共5页
[Objectives] To provide experimental basis for the effective development and utilization of Huoshan large-leaf yellow tea resources and the screening of safe and effective active ingredients of large-leaf yellow tea. ... [Objectives] To provide experimental basis for the effective development and utilization of Huoshan large-leaf yellow tea resources and the screening of safe and effective active ingredients of large-leaf yellow tea. [Methods] The active substances of Huoshan large-leaf yellow tea were extracted by hot-water extraction, and the freeze-dried powder of Huoshan large-leaf yellow tea was obtained by freeze drying. The antibacterial activity of the extract was preliminarily confirmed using the Oxford cup method, and its antimicrobial spectrum was analyzed using 14 strains. A xylene-induced mouse auricle swelling test was carried out to detect the swelling inhibition rate of the extract and analyze its in-vitro detumescent activity. Then, the antioxidant activity of the extract was identified through a DPPH free radical scavenging capacity test and a ferric reducing antioxidant power assay. [Results] The extract had significant inhibitory effects on various bacteria. The extract could effectively inhibit the growth of Escherichia coli, Staphylococcus aureus, Enterococcus hirae, Acinetobacter baumannii, Klebsiella pneumonia, Pseudomonas aeruginosa, Bacillus subtilis , and other strains. The diameter of the inhibition zone increased with the increase of sample concentration. The extract had a significant inhibitory effect on auricle swelling induced by xylene in mice. When the concentration of the drug reached 1.0 mg/mL, its inhibition rate on mouse auricle swelling reached 55.2% ( P <0.01), slightly lower than the swelling inhibition rate of the aspirin group (66.52%, P <0.01). The results of the antioxidant test showed that large-leaf yellow tea extract also had strong activity. Within the concentration range of 0.1-1.0 mg/mL, its DPPH radical scavenging rate increased with the increase of sample concentration. Within the concentration range of 0.1-1.0 mg/mL, its DPPH radical scavenging rate increased with the increase of sample concentration. When the concentration reached 1.0 mg/mL, the scavenging rate reached 69.75%. The Fe 3+ -reduction capacity of the extract also increased with the increase of sample concentration within the concentration range of 0.1-2.5 mg/mL. When the concentration was 2.5 mg/mL, the reducing power of the extract reached 1.43±0.04. However, its DPPH free radical scavenging rate and reducing power were slightly lower than the capacity of V C at the same concentration. [Conclusions] The extract of Huoshan large-leaf yellow tea obtained by hot-water extraction had strong activity in many aspects, including inhibiting the growth of various microbes, subsiding swelling in vitro and resisting oxidation. These experimental results provide certain guiding significance for the basic research of Huoshan large-leaf yellow tea extract, as well as experimental data support for the subsequent development of functional foods and drugs of Huoshan large-leaf yellow tea. 展开更多
关键词 Huoshan large-leaf yellow tea Antibacterial activity DETUMESCENCE Antioxidant activity
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Expression and Purification of Human Coagulation Factor X in Mammalian CHO-DG44 Cells
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作者 Jinwu CHEN Yi LI +4 位作者 Mei LIU Sainan WANG Zilong XIAO Junjie XIA Lulu QI 《Agricultural Biotechnology》 CAS 2023年第3期50-54,共5页
[Objectives]This study was conducted to obtain a Chinese hamster ovary cell line that stably expresses recombinant human coagulation factor X(rhFX),and to induce efficient expression of the target gene with different ... [Objectives]This study was conducted to obtain a Chinese hamster ovary cell line that stably expresses recombinant human coagulation factor X(rhFX),and to induce efficient expression of the target gene with different concentrations of methotrexate(MTX).[Methods]PCR was performed to obtain the rhFX gene,and a recombinant expression plasmid pOptiVEC-rhFX was constructed and subjected to double restriction endonuclease digestion and sequencing identification.CHO-DG44(DHFR-)cells were transfected by the liposome method,and the target protein was purified by affinity chromatography and detected by SDS-PAGE electrophoresis and Western blot.A cell line with efficient and stable expression of the target gene was obtained by increasing the concentration of MTX to select positive clones.[Results]PCR yielded a 1509 bp rhFX sequence,and the results of double digestion and sequencing showed that the constructed pOptiVEC-rhFX plasmid was correct.After transfection of cells,MTX significantly increased protein expression.When MTX reached 1.0μmol/L,the expression efficiency of the target protein was(9±0.27)μg/ml.The purity of the target protein purified by affinity chromatography was 93%,which could be used for subsequent experiments.The expression efficiency of rhFX in eukaryotic mammalian cells was improved by increasing MTX concentration,and an affinity chromatography purification process for the target protein was preliminarily established.[Conclusions]The results of this study provide data support for the expression and purification of rhFX,and will lay a solid foundation for the development of drugs related to rhFX. 展开更多
关键词 Recombinant human coagulation factor X(rhFX) Eukaryotic expression MTX Affinity chromatography
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Molecular epidemiology of piliated pneumococcal isolates at a major tertiary hospital in the Klang Valley,Malaysia
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作者 Nurul Diana Dzaraly Mohd Nasir Mohd Desa +11 位作者 AbdulRahman Muthanna Siti Norbaya Masri Niazlin Mohd Taib Nurul Hana Zainal Baharin Nurshahira Sulaiman Nurul Asyikin Abdul Rahman Navindra Kumari Palanisamy Zarizal Suhaili Semiat Opeyemi Yusuff Nor Iza A.Rahman Tuan Suhaila Tuan Soh Fatimah Haslina Abdullah 《Asian Pacific Journal of Tropical Medicine》 SCIE CAS 2023年第2期80-88,共9页
Objective:To characterise a collection of pili-carrying and none pili-carrying pneumococcal isolates of clinical origin for serotypes,antibiotic resistance and genotype.Methods:In total,42 clinical isolates were colle... Objective:To characterise a collection of pili-carrying and none pili-carrying pneumococcal isolates of clinical origin for serotypes,antibiotic resistance and genotype.Methods:In total,42 clinical isolates were collected between October 2017 and December 2019.Those isolates were analysed for antimicrobial susceptibility,serotype distribution,detection of pneumococcal virulence and pilus genes.Multilocus sequence typing was performed only for piliated isolates,followed by phylogenetic analysis.Results:The common isolation sites among the pneumococcal isolates were tracheal aspirate(28.6%),blood(26.2%),and sputum(23.8%).Fifty percent isolates were resistant to erythromycin,tetracycline(50.0%)and trimethoprim-sulfamethoxazole(43.0%).The most frequent were serotypes 19F(28.6%),6A/B(23.8%)and 19A(14.3%).Piliated isolates were detected in a small proportion(33.3%);64.3%were multidrug-resistant.ST320 was the prevalent sequence type among the piliated isolates and genetically related to the Pneumococcal Molecular Epidemiology Network clones Taiwan19F-14(CC271).In the phylogenetic analysis,some piliated isolates showed a close association having similar ST320,carrying serotype 19A and both pilus genes indicating their clonal spread.Conclusions:Pneumococcal lineages of piliated isolates have been globally disseminated and pili could have played a role in the spread of antibiotic resistant clones. 展开更多
关键词 Pneumococci PILI GENOTYPE MALAYSIA
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Recent development of LiNi_xCo_yMn_zO_2:Impact of micro/nano structures for imparting improvements in lithium batteries 被引量:8
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作者 潘成迟 Craig E.BANKS +3 位作者 宋维鑫 王驰伟 陈启元 纪效波 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2013年第1期108-119,共12页
The recent advancement in the design,synthesis,and fabrication of micro/nano structured LiNixCoyMnzO2 with one-,two-,and three-dimensional morphologies was reviewed.The major goal is to highlight LiNixCoyMnzO2 materia... The recent advancement in the design,synthesis,and fabrication of micro/nano structured LiNixCoyMnzO2 with one-,two-,and three-dimensional morphologies was reviewed.The major goal is to highlight LiNixCoyMnzO2 materials,which have been utilized in lithium ion batteries with enhanced energy and power density,high energy efficiency,superior rate capability and excellent cycling stability resulting from the doping,surface coating,nanocomposites and nano-architecturing. 展开更多
关键词 lithium-ion battery micro/nano structures LiNixCoyMnzO2 DOPING surface coating composite materials
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