Tao and Golden Ratio: A Scientific View of Contemporary Acupunctural Principles through Geometry

It is universally accepted that the philosophy of the Tao is the essence that animates the Chinese Cosmogony. Therefore, in last decades I have tried to consolidate its scientific background, looking for solid explanations through Exact Sciences (“Between Heaven and Earth” Scientific Basis of the Action of Shao Yin: Lightning’s Physical-Mathematical Analysis”;“Is Traditional Chinese Medicine Definitely an Exact Science?”;“Euclidean Geometry and Traditional Chinese Medicine: Diving into the Real Origin of the Five Elements”;“Solitons: A Cutting-Edge Scientific Proposal Explaining the Mechanisms of Acupuntural Action”) Currently, research on Chinese medicine leads us—with Dr. Erica Arakaki, collaborator and assistant—to verify through a profound bibliographic review the application of Fibonacci’s Golden Ratio in the constitution of T’ai Ji Tu, adding yet more substance to the hypothesis of Acupuncture and demonstrating how said Chinese Ancient Science is not only an empirical knowledge but a wisdom derived from the most ancient exact science: Geometry.

Two missense STK11 gene variations impaired LKB1/adenosine monophosphate-activated protein kinase signaling in Peutz-Jeghers syndrome

BACKGROUND Peutz-Jeghers syndrome(PJS)is a rare hereditary neoplastic disorder mainly associated with serine/threonine kinase 11(STK11/LKB1)gene mutations.Preimplantation genetic testing can protect a patient’s offspring from mutated genes;however,some variations in this gene have been interpreted as variants of uncertain significance(VUS),which complicate reproductive decision-making in genetic counseling.AIM To identify the pathogenicity of two missense variants and provide clinical guidance.METHODS Whole exome gene sequencing and Sanger sequencing were performed on the peripheral blood of patients with PJS treated at the Reproductive and Genetic Hospital of Citic-Xiangya.Software was employed to predict the protein structure,conservation,and pathogenicity of the two missense variation sites in patients with PJS.Additionally,plasmids were constructed and transfected into HeLa cells to observe cell growth.The differences in signal pathway expression between the variant group and the wild-type group were compared using western blot and immunohistochemistry.Statistical analysis was performed using one-way analysis of variance.P<0.05 was considered statistically significant.RESULTS We identified two missense STK11 gene VUS[c.889A>G(p.Arg297Gly)and c.733C>T(p.Leu245Phe)]in 9 unrelated PJS families who were seeking reproductive assistance.The two missense VUS were located in the catalytic domain of serine/threonine kinase,which is a key structure of the liver kinase B1(LKB1)protein.In vitro experiments showed that the phosphorylation levels of adenosine monophosphate-activated protein kinase(AMPK)at Thr172 and LKB1 at Ser428 were significantly higher in transfected variation-type cells than in wild-type cells.In addition,the two missense STK11 variants promoted the proliferation of HeLa cells.Subsequent immunohistochemical analysis showed that phosphorylated-AMPK(Thr172)expression was significantly lower in gastric,colonic,and uterine polyps from PJS patients with missense variations than in non-PJS patients.Our findings indicate that these two missense STK11 variants are likely pathogenic and inactivate the STK11 gene,causing it to lose its function of regulating downstream phosphorylated-AMPK(Thr172),which may lead to the development of PJS.The identification of the pathogenic mutations in these two clinically characterized PJS patients has been helpful in guiding them toward the most appropriate mode of pregnancy assistance.CONCLUSION These two missense variants can be interpreted as likely pathogenic variants that mediated the onset of PJS in the two patients.These findings not only offer insights for clinical decision-making,but also serve as a foundation for further research and reanalysis of missense VUS in rare diseases.

Optimization Study of Active-Passive Heating System Parameters in Village Houses in the Southern Xinjiang Province

Aiming at the problems of large energy consumption and serious pollution of winter heating existing in the rural buildings in Southern Xinjiang,a combined active-passive heating system was proposed,and the simulation software was used to optimize the parameters of the system,according to the parameters obtained from the optimization,a test platform was built and winter heating test was carried out.The simulation results showed that the thickness of the air layer of 75 mm,the total area of the vent holes of 0.24 m^(2),and the thickness of the insulation layer of 120 mm were the optimal construction for the passive part;solar collector area of 28 m^(2),hot water storage tank volume of 1.4 m^(3),mass flow rate of 800 kg/h on the collector side,mass flow rate of 400 kg/h on the heat exchanger side,and output power of auxiliary heat source of 5∼9 kWwere the optimal constructions for active heating system.Test results showed that during the heating period,the system could provide sufficient heat to the room under different heating modes,and the indoor temperature reached over 18°C,which met the heating demand.The economic and environmental benefits of the system were analyzed,and the economic benefits of the systemwere better than coal-fired heating,and the CO_(2) emissionswere reduced by 3,292.25 kg compared with coalfiredheating.The results of the study showed that the combinedactive-passiveheating systemcouldeffectively solve the heating problems existing in rural buildings in Southern Xinjiang,and it also laid the theoretical foundation for the popularization of the combined heating systems.

Multi-omics Approach Reveals Influenza-A Virus Target Genes Associated Genomic,Clinical and Immunological Characteristics in Cancers

Objective To examine the precise function of influenza A virus target genes(IATGs)in malignancy.Methods Using multi-omics data from the TCGA and TCPA datasets,33 tumor types were evaluated for IATGs.IATG expression in cancer cells was analyzed using transcriptome analysis.Copy number variation(CNV)was assessed using GISTICS 2.0.Spearman’s analysis was used to correlate mRNA expression with methylation levels.GSEA was used for the enrichment analysis.Pearson’s correlation analysis was used to examine the association between IATG mRNA expression and IC50.The ImmuCellAI algorithm was used to calculate the infiltration scores of 24 immune cell types.Results In 13 solid tumors,IATG mRNA levels were atypically expressed.Except for UCS,UVM,KICH,PCPG,THCA,CHOL,LAMI,and MESO,most cancers contained somatic IATG mutations.The main types of CNVs in IATGs are heterozygous amplifications and deletions.In most tumors,IATG mRNA expression is adversely associated with methylation.RT-PCR demonstrated that EGFR,ANXA5,CACNA1C,CD209,UVRAG were upregulated and CLEC4M was downregulated in KIRC cell lines,consistent with the TCGA and GTEx data.Conclusion Genomic changes and clinical characteristics of IATGs were identified,which may offer fresh perspectives linking the influenza A virus to cancer.

Statistical Analysis and Research on the Authorized Patent of Donghua University

With retrieving the granted patents in 2002-2011 in Donghua University (DHU), the authorized invention patent distribution among colleges and subjects is introduced in this paper, based on the recorded patent database of the China State Intellectual Property Office. This paper can provide useful experiences in the discipline construction, benefiting the characteristics disciplines industry group and multidisciplinary infiltration and research cooperation. In addition, it encourages teachers and students to get more intellectual property rights for schools and improves the overall strength of the university.

Research on the mechanism of Chaihu Huangqin medicine based on integrated pharmacology to improve the clinical symptoms of COVID-19

Objective:Novel coronavirus(COVID-19)was used to improve the clinical symptoms of the new type coronavirus(LV),based on integrated pharmacology.Methods:TCMIP v2.0 was used to predict the key target and action pathway of Bupleurum baicalensis Georgi in improving the clinical symptoms of covid-19.The multi-dimensional network diagram of"drug pair active component key target action pathway"was drawn to explore its action mechanism from the molecular level.Results:Bupleurum and Scutellaria baicalensis could regulate IL signaling pathway and clec7a(a member of C-type lectin domain 7 family)/Inflammatory pathway,immune stress and other related signaling pathways can inhibit activated cytokines,alleviate excessive immune response,eliminate inflammation,and resist virus.In addition,the core targets of Bupleurum baicalensis baicalensis Georgi:nuclear factor kB(NFKB2,NFKB1),interleukin(IL-6,IL-1B),toll like receptor 4(TLR4),tumor necrosis factor(TNF)and so on are mostly induced by inflammation It is related to body damage.Bupleurum and scutellaria have the clinical effects of anti-inflammatory,antiviral,strengthening immunity,inhibiting pulmonary fibrosis and so on.Conclusion:Radix Bupleuri Scutellariae can be used to treat covid-19 by multi-channel,multi-target and multi ring.The results of this study provide a theoretical basis for Bupleurum Scutellaria to improve the use of COVID-19.

Inactivation of ERK1/2-CREB Pathway Is Implicated in MK801-induced Cognitive Impairment

Objective Schizophrenia(SZ)is associated with cognitive impairment,and it is known that the activity of cAMP response element binding protein(CREB)decreases in the brain of SZ patients.The previous study conducted by the investigators revealed that the upregulation of CREB improves the MK801-related SZ cognitive deficit.The present study further investigates the mechanism on how CREB deficiency is associated with SZ-related cognitive impairment.Methods MK-801 was used to induce SZ in rats.Western blotting and immunofluorescence were performed to investigate CREB and the CREB-related pathway implicated in MK801 rats.The long-term potentiation and behavioral tests were performed to assess the synaptic plasticity and cognitive impairment,respectively.Results The phosphorylation of CREB at Ser133 decreased in the hippocampus of SZ rats.Interestingly,among the upstream kinases of CREB,merely ERK1/2 was downregulated,while CaMKII and PKA remained unchanged in the brain of MK801-related SZ rats.The inhibition of ERK1/2 by PD98059 reduced the phosphorylation of CREB-Ser133,and induced synaptic dysfunction in primary hippocampal neurons.Conversely,the activation of CREB attenuated the ERK1/2 inhibitor-induced synaptic and cognitive impairment.Conclusion These present findings partially suggest that the deficiency of the ERK1/2-CREB pathway is involved in MK801-related SZ cognitive impairment.The activation of the ERK1/2-CREB pathway may be therapeutically useful for treating SZ cognitive deficits.

Conflict management styles of nurse managers and influencing factors in children’s hospital, Vietnam

Objective:This study aimed to describe the conflict management styles and identify factors affecting the conflict management styles of nurse managers.Materials and Methods:A cross-sectional study was conducted by interviewing nurse managers with a questionnaire,including the Rahim Organizational Conflict Inventory-II Form B,used to assess an individual’s approach to conflict management across five styles:cooperating,competing,compromising,obliging,and avoiding.Results:A total of 30 nurse managers participated in this study.The mean scores of the cooperating style were the highest at 4.3±0.4,followed by a compromising style at 3.4±0.5,an avoiding style at 3.1±0.6,an accommodating style at 2.9±0.7,and a competing style at 2.8±0.8(χ^(2)=66.624,P<0.001).The mean scores for avoiding style among bachelor’s degree nurse managers(3.2±0.6)were higher than those of master’s degree nurse managers(2.7±0.6)(P=0.036).Nurse managers over 40 years old,holding a managerial position for≥10 years,and frequent conflict handling rated higher mean scores for the cooperating style than their counterparts(P<0.05).Conclusion:The cooperating style was the most commonly used conflict management style among nurse managers.Factors affecting the conflict management style were age,education level,and experience in management and conflict handling.These findings are useful for developing interventions that enhance the competencies of nurse managers in conflict management.

Multi-attribute decision making method for air target threat evaluation based on intuitionistic fuzzy sets

The function of the air target threat evaluation （TE） is the foundation for weapons allocation and senor resources management within the surface air defense. The multi-attribute evaluation methodology is utilized to address the issue of the TE in which the tactic features of the detected target are treated as evaluation attributes. Meanwhile, the intuitionistic fuzzy set （IFS） is employed to deal with information uncertainty in the TE process. Furthermore, on the basis of the entropy weight and inclusion-comparison probability, a hybrid TE method is developed. In order to accommodate the demands of naturalistic decision making, the proposed method allows air defense commanders to express their intuitive opinions besides incorporating into the threat features of the detected target. An illustrative example is provided to indicate the feasibility and advantage of the proposed method.

Effects of Exercise on Behavior and Peripheral Blood Lymphocyte Apoptosis in a Rat Model of Chronic Fatigue Syndrome

This study examined the effects of exercise on behavior and peripheral blood leukocyte apoptosis in a rat model of chronic fatigue syndrome （CFS）. Thirty-six healthy male Sprague-Dawley rats were equally randomized into 3 groups： the control group, CFS model group and the exercise group in terms of body weight. A total of 25 rats entered the final statistical analysis due to 11 deaths during the study. CFS model was established by subjecting the rats in CFS model group and exercise group to electric shock, chronic restraint stress and cold water swim. Besides, rats in the exercise group took rtmning wheel exercise. After a week of conditioning feeding, model construction and running wheel exercise were performed simultaneously, and lasted for 23 consecutive days. The behavior experiments, including running wheel exercise, open-field test, tail suspension test and Morris water maze test, were conducted, either before or after the model establishment. Rats were sacrificed and peripheral blood was obtained for the assessment of lymphocyte apoptosis index by flow cytometry （FCM）. It was found that as compared with those in the control group, the weight of the rats was decreased obviously （P〈0.01）, the mobility time in the open-field and the tail suspension tests was shortened significantly （P〈0.01）, the time to locate the platform was enhanced （P〈0.01） and the cell apoptosis index was increased substantially （P〈0.01） in the CSF model group. Meanwhile, in comparison to the model group, the behavior in the open-field and the tail suspension tests was improved significantly （P〈0.05）, and the apoptosis index decreased remarkably （P〈0.01） in the exercise group. It is concluded that sport intervention can prevent lymphocyte apoptosis and improve animal behavior rather than the memory.

Analysis of the TCM theory of traditional Chinese health exercise

The traditional Chinese medicine(TCM)theory of traditional Chinese health exercise(TCHE)is analyzed.The effect of meridians and acupoints contained in TCHE is summarized,the treatment methods of tonifying deficiencies and reducing excesses applied in TCHE are discussed,the regulation of Zang-fu organs are emphasized,and the leading role of Qi is introduced.The exercise prescription and proper timing of TCHE are described and reported.TCHEs,such as Baduanjin,Liuzijue,and Tai Chi,possess strong TCM characteristics.

Baicalin inhibits colistin sulfate-induced apoptosis of PC12 cells

Baicalin, a type of flavonoid extracted from the dried root of Scutellaria baicalensis georgi, has been shown to effectively inhibit cell apoptosis. Therefore, we assumed that baicalin would suppress colistin sulfate-induced neuronal apoptosis. PC12 cells exposed to colistin sulfate （62.5-500 μg/mL） for 24 hours resulted in PCl2 cell apoptosis. In addition, caspase-3 activity, lactate dehydrogenase level and free radical content increased in a dose-dependent manner. Subsequently, PC12 cells were pretreated with baicalin （25, 50 and 100 pg/mL）, and exposed to 125 pg/mL colistin sulfate. Cell morphology markedly changed, and cell viability increased. Moreover, caspase-3 activity, lac- tate dehydrogenase level and free radical content decreased. Results indicated that baicalin inhib- ited colistin sulfate-induced PC12 cell apoptosis by suppressing free radical injury, and reducing caspase-3 activity and lactate dehydrogenase activity.

An optimizing algorithm of static task scheduling problem based on hybrid genetic algorithm

To reduce resources consumption of parallel computation system, a static task scheduling opti- mization method based on hybrid genetic algorithm is proposed and validated, which can shorten the scheduling length of parallel tasks with precedence constraints. Firstly, the global optimal model and constraints are created to demonstrate the static task scheduling problem in heterogeneous distributed computing systems（HeDCSs）. Secondly, the genetic population is coded with matrix and used to search the total available time span of the processors, and then the simulated annealing algorithm is introduced to improve the convergence speed and overcome the problem of easily falling into local minimum point, which exists in the traditional genetic algorithm. Finally, compared to other existed scheduling algorithms such as dynamic level scheduling （ DLS）, heterogeneous earliest finish time （HEFr）, and longest dynamic critical path（ LDCP）, the proposed approach does not merely de- crease tasks schedule length, but also achieves the maximal resource utilization of parallel computa- tion system by extensive experiments.

Three-dimensional visualization of the functional fascicular groups of a long-segment peripheral nerve

The three-dimensional（3D） visualization of the functional bundles in the peripheral nerve provides direct and detailed intraneural spatial information. It is useful for selecting suitable surgical methods to repair nerve defects and in optimizing the construction of tissue-engineered nerve grafts. However, there remain major technical hurdles in obtaining, registering and interpreting 2D images, as well as in establishing 3D models. Moreover, the 3D models are plagued by poor accuracy and lack of detail and cannot completely reflect the stereoscopic microstructure inside the nerve. To explore and help resolve these key technical problems of 3D reconstruction, in the present study, we designed a novel method based on re-imaging techniques and computer image layer processing technology. A 20-cm ulnar nerve segment from the upper arm of a fresh adult cadaver was used for acetylcholinesterase（ACh E） staining. Then, 2D panoramic images were obtained before and after ACh E staining under the stereomicroscope. Using layer processing techniques in Photoshop, a space transformation method was used to fulfill automatic registration. The contours were outlined, and the 3D rendering of functional fascicular groups in the long-segment ulnar nerve was performed with Amira 4.1 software. The re-imaging technique based on layer processing in Photoshop produced an image that was detailed and accurate. The merging of images was accurate, and the whole procedure was simple and fast. The least square support vector machine was accurate, with an error rate of only 8.25%. The 3D reconstruction directly revealed changes in the fusion of different nerve functional fascicular groups. In conclusion. The technique is fast with satisfactory visual reconstruction.

DFT Investigation on the Insertion Reaction of an Isocyanide into the(Silyl)(silylene)molybdenum Si-C Bond

The chemistry of silylene-transition-metal complexes LnM（=SiR2）SiR3 is one of the attractive synthetic targets in organometallic chemistry for such complexes are key intermediates in many transition metal-catalyzed systems.In the present work,the novel insertion reactions of an isocyanide into the Cp＊Mo（CO）2（=SiMe2）（SiMe3） Si-C bond were investigated extensively by using the DFT method.The effective core potentials（ECP） with a double-ζ valence basis set（LanL2DZ） were employed for Mo and Si and the standard 6-31G basis set for all other atoms.Polarization functions[22] were added for Si（ζd = 0.262）,C（ζd = 0.8） and N（ζd = 0.8）.The effect of solvent was evaluated with the standard PCM model.Our calculations show that the two-step channel is energetically favorable,and the solvation mode has minor influence on the relative energies.

Integration of naval distributed tactical training simulation system based on advanced message queuing protocol

Aiming at the problems of unreliable data transmission,poor steadiness,nonsupport of complex data types,direct couple between data transmission and exchange,a high-level method based on advanced message queuing protocol( AMQP) is proposed to integrate naval distributed tactical training simulation system after serious consideration with current information exchange features of military combat system. Transferring layer in traditional user datagram protocol is implemented by publishing and subscribing scheme of message middleware. By creating message model to standardize message structure,integration architecture is formulated to resolve potential information security risks from inconsistent data type and express data transmission. Meanwhile,a communication model is put forward based on AMQP,which is in the center position of the whole transmission framework and responsible for reliably transferring battlefield data among subsystems. Experiments show that the method can accurately post amounts of data to the subscriber without error and loss,and can get excellent real-time performance of data exchange.

The Difficulties and Countermeasures of Migrant Workers Returning Home to Start Business of Shaanxi

The paper discloses the current situation of migrant workers returning home to start business by making a survey of the total number of returning migrant workers. The paper also points out the main difficulties the migrant workers who returning home to start business facing. The first is the old fashioned and backward concepts of development in some local departments; the second is lacking of powerful supports of policies; the third is the still bad environment to start business; the fourth is the lower qualities of people who are going to start business. Based on these situations, the official policies are put forward to encourage and support migrant workers to return home to start business. Such as strategies activating migrant workers to return home to start business should be positively operated; the management services should be further standardized and the environment should be perfected for starting business; the programs and items to start business by migrant workers returning home should be guided and favored; beneficial tax policies should be improved further; financial services should be further perfected; the land using problems of migrant workers should be firstly solved; the demonstration project of migrant workers returning back to start business should be continuously carried on; the organizing and leading jobs for starting business of migrant workers should be further strengthened.

Compound Chaijin Jieyu Tablets ameliorating insomnia complicated with depression by improving synaptic plasticity via regulating orexin A,melatonin,and acetylcholine contents

Objective To investigate the efficacy and mechanism of action of Compound Chaijin Jieyu Tablets(复方柴金解郁片,CCJJYT)in rats with insomnia complicated with depression.Methods Seventy-two Sprague-Dawley rats were randomly assigned into eight groups:the control,chronic unpredictable mild stress(CUMS),sleep deprivation(SD),CUMS+SD,positive drug(venlafaxine hydrochloride+diazepam),CCJJYT high-dose(CCJJYT˗2×),medium-dose(CCJJYT˗1×),and low-dose(CCJJYT˗0.5×)groups,with nine rats in each group.Depression-like behavior was evaluated by body weight,food intake,and behavioral tests such as the sucrose preference test(SPT),open field test(OFT),forced swimming test(FST),and pentobarbital-induced sleep test(PST).Hematoxylin-eosin(HE)staining and Golgi-Cox staining were used to observe changes in pathological tissue and synaptic morphology,respectively.Enzyme-linked immunosorbent assay(ELISA)was used to detect the contents of orexin-A and acetylcholine.The expression levels of orexin receptor 1(OXR1),melatonin receptor 1(MT1A),melatonin receptor 2(MT1B),acetylcholinesterase(AChE),and choline acetyltransferase(ChAT)were detected by immunohistochemistry and Western blot.Results In the present study,rats in the model group showed significant behavioral changes as well as a reduction in hippocampal dendritic branch length and synaptic number,along with increasing the content of orexin A and acetylcholine(P<0.05),and altered expression levels of OX1R,MT1A,MT1B,ChAT,and AChE in the hippocampus and prefrontal cortex after modeling(P<0.05).CCJJYT can improve depressive insomnia behavior and synaptic plasticity of rats(P<0.05),which is similar to that of the positive drug group.It can also decrease the content of orexin A and acetylcholine,and reduce the expression levels of OXR1 and ChAT in hippocampus and prefrontal cortex(P<0.05),and increase the expression levels of MT1A,MT1B,and AChE proteins(P<0.05).Conclusion CCJJYT has good antidepressant and insomnia effects,probably through the regulation of orexin-A,melatonin,and acetylcholine content in hippocampus and prefrontal cortex of rats,improving synaptic plasticity and thus exerting antidepressant and insomnia effects.

Synergetic effect of nitrogen‐doped carbon catalysts for high‐efficiency electrochemical CO_(2) reduction

The use of carbon‐based materials is an appealing strategy to solve the issue of excessive CO_(2) emis‐sions.In particular,metal‐free nitrogen‐doped carbon materials(mf‐NCs)have the advantages of convenient synthesis,cost‐effectiveness,and high conductivity and are ideal electrocatalysts for the CO_(2) reduction reaction(CO_(2)RR).However,the unclear identification of the active N sites and the low intrinsic activity of mf‐NCs hinder the further development of high‐performance CO_(2)RR electrocat‐alysts.Achieving precise control over the synthesis of mf‐NC catalysts with well‐defined active N‐species sites is still challenging.To this end,we adopted a facile synthesis method to construct a set of mf‐NCs as robust catalysts for CO_(2)RR.The resulting best‐performing catalyst obtained a Far‐adaic efficiency of CO of approximately 90%at−0.55 V(vs.reversible hydrogen electrode)and good stability.The electrocatalytic performance and in situ attenuated total reflectance surface‐enhanced infrared absorption spectroscopy measurements collectively revealed that graphitic and pyridinic N can synergistically adsorb CO_(2) and H_(2)O and thus promote CO_(2) activation and protonation.

Gender identification using acoustic analysis in birds without external sexual dimorphism

Zoo and wildlife management faces a problem with bird sexing,as many bird taxa have indiscernible gender differences in size and coloration.Problematic groups are geese,cranes,rails,raptors,owls,parrots,doves,auks,shearwaters and some passerines.Commonly accepted invasive sexing techniques based on genetics,laparoscopy,morphometric and on cloacal inspection,are all needed in bird capturing and handling.Capturing and subsequent manipulations may be inapplicable for free-ranging birds,whereas distant voice-based sexing is relevant for many species.This review evaluates the potential for noninvasive sexing by separate calls or duet calls,for adult birds of 69 species from 16 orders and for chicks of 11 species from 7 orders.For adult birds of 25 species,a single call per individual was sufficient for 100 % reliable sexing by ear or using spectrographic analysis.For chicks,the potential for voice-based sexing seems to be very limited.For birds calling rarely or unpredictably,we propose a simple way of provoking vocalization using playbacks of species-specific calls that are available from sound libraries.We conclude that sexing by voice may represent a feasible alternative to the classical sexing techniques,both in the wild and in captivity.