Genome-Wide Identification of Tomato (Solanum lycopersicum L.) CKX Gene Family and Expression Analysis in the Callus Tissue under Zeatin Treatment

The cytokinin oxidase/dehydrogenase(CKX)enzyme is essential for controlling thefluctuating levels of endogen-ous cytokinin(CK)and has a significant impact on different aspects of plant growth and development.Nonethe-less,there is limited knowledge about CKX genes in tomato(Solanum lycopersicum L.).Here we performed genome-wide identification and analysis of nine SlCKX family members in tomatoes using bioinformatics tools.The results revealed that nine SlCKX genes were unevenly distributed onfive chromosomes(Chr.1,Chr.4,Chr.8,Chr.10,and Chr.12).The amino acid length,isoelectric points,and molecular weight of the nine SlCKX proteins ranged from 453 to 553,5.77 to 8.59,and 51.661 to 62.494 kD,respectively.Subcellular localization analysis indi-cated that SlCKX2 proteins were located in both the vacuole and cytoplasmic matrix;SlCKX3 and SlCKX5 pro-teins were located in the vacuole;and SlCKX1,4,6,7,8,and 9 proteins were located in the cytoplasmic matrix.Furthermore,we observed differences in the gene structures and phylogenetic relationships of SlCKX proteins among different members.SlCKX1-9 were positioned on two out of the three branches of the CKX phylogenetic tree in the multispecies phylogenetic tree construction,revealing their strong conservation within phylogenetic subgroups.Unique patterns of expression of CKX genes were noticed in callus cultures exposed to varying con-centrations of exogenous ZT,suggesting their roles in specific developmental and physiological functions in the regeneration system.These results may facilitate subsequent functional analysis of SlCKX genes and provide valu-able insights for establishing an efficient regeneration system for tomatoes.

Identification of key taste components in Baccaurea ramiflora Lour.fruit using non-targeted metabolomics

Among the numerous fruit species of Baccaurea ramiflora Lour.,‘LR’(white flesh)and‘BR’(pink flesh)are two kinds of local strains with high edibility.In order to study the metabolic causes of taste differences,we performed the non-targeted metabonomics analysis of‘LR’and‘BR’using LC-MS/MS.541 metabolites were totally identified,and 45 kinds of metabolites(carbohydrates,fatty acids,flavonoids and terpenoids,etc.)were different between the two strains.The results indicate L-sorbose,D-(+)-glucose,citric acid,L-phenylalanine and oleamide,α-eleostearic acid were the main primary metabolites.The significant difference existed in pathways of unsaturated fatty acids between the studied two strains by pathway enrichment analysis.The results demonstrate that the different in composition,as well as the abundance of primary and secondary metabolites may be the potential causes of taste differences,which provides a new insight into the possible metabolic factors setting off the changing taste of B.ramiflora.

Comprehensive Evaluation of New Self-cultivated Sugarcane Lines by Grey Relational Analysis

The grey relational analysis( GRA) was used to evaluate the 10 sugarcane lines by 13 main agronomic characters. The result showed that the grey correlation degree of self-cultivated lines GNY08- 320(0. 7502),GNY08- 336(0. 7409),GNY08- 186(0. 7369),and GNY08- 225(0. 7277) were higher than ROC22(CK),so the 4 lines can be further tested,and the others were worse than CK that should be further observed or eliminated.

Chromosomal-level genome of macadamia(Macadamia integrifolia)

Macadamia from the family Proteaceae is a plant native to Australia and has long been favoured by people for its crispy and high nutritional and medicinal value.Here,the genome of GUIRE 1(GR1),a highly heterozygous superior cultivar of macadamia nut,was sequenced and assembled using nanopore sequencing,and a 807-Mb genome(contig N50,1.9 Mb;scaffold N50,54.70 Mb)and 14 chromosomes were obtained.A total of 453 Mb(about 55.95%)repetitive sequences and 37,657 protein-coding genes were obtained by gene annotation and homologous protein comparison.Proteaceae diverged from Nelumbonaceae nearly 115.37 million years ago and from Rubiaceae about 140 million years ago.A genome-wide duplication(WGD)event occurred in macadamia 41 million years ago based on the WGD analysis.The functional enrichment analysis of M.integrifolia-specific gene families revealed their roles in signal transduction,protein phosphorylation,protein binding,and defense response.Here,a highly heterozygous genome of M.integrifolia was unlocked to provide a database for breeding and molecular mechanism research.

Resistance Selection Against Abamectin in Tetranychus cinnabarinus(Boisduval) and Changes in Its Detoxification Enzyme Activity

The susceptible strain of Tetranychus cinnabarinus (Boisduval) was collected from the cassava germplasm resource garden of Guangxi South Subtropical Agricultural Science Research Institute and raised for 15 generations,during which resistance selection against avermectin was performed through the indoor spraying of avermectin,thereby obtaining an avermectin-resistance strain (Ab-R) with a resistance ratio of 3.25.After the indoor resistance selection,the determination of detoxification enzyme activity in the Ab-R and SS strains showed that the specific activity of carboxylesterases (CarE),glutathione-s-transferases (GSTs) and multi-functional oxidase (MFO) in Ab-R15 was 1.27,1.69 and 1.92 times of that in SS,respectively.In addition,the specific activity of MFO in Ab-R5,Ab-R10 and Ab-R15 was significantly different from that in SS;there was no significant difference in the specific activity of CarE compared with SS when screened to the F10 generation;and when screened to the F5 generation,there was no significant difference in the specific activity of GSTs compared with SS.The results showed that the significant increase in MFO activity was an important cause of resistance to avermectin in T.cinnabarinus,and CarE and GSTs were also involved in the formation of avermectin-resistant strains.

Toxicity and Field Efficacy Test of Several Insecticides against Carsidara marginalis Walker

[Objective]The paper was to study the prevention and control effect of different chemical insecticides on Carsidara marginalis Walker.[Method]The indoor toxicity and field efficacy of six insecticides against C.marginalis were determined by residual film method and field efficacy test.The toxicity and field efficacy were comparatively analyzed.[Result]The LC50 of 5%abamectin EC,24%spirotetramat EC,10%lambdacyhalothrin EC,10%nitenpyram SL,25%pymetrozine WP and 20%imidacloprid EC against C.marginalis were 3.585,12.083,15.012,18.754,23.373 and 61.161 mg/L,respectively.The field efficacies against C.marginalis at 1 d post administration were 78.04%,80.05%,69.92%,82.27%,71.72%and 76.72%,respectively.The field efficacies against C.marginalis at 10 d post administration were 92.49%,82.41%,83.80%,88.24%,94.10%and 97.01%,respectively.[Conclusion]The agents with strong quick effect and insecticides with strong systematic conductivity should be mixed together,in order to achieve quick and continuous insecticidal effects.

Species and Occurrence Regularity of Diseases and Insect Pests Harming Baccaurea ramiflora Lour. in Guangxi

[Objectives]The paper was to understand the species and harm of diseases and insect pests of Baccaurea ramiflora Lour.in Guangxi,and to provide basis for the prevention and control of diseases and insect pests.[Methods]From 2018 to 2021,a systematic investigation on diseases and insect pests of B.ramiflora was conducted in Dongxing City,Fangchenggang City,Longzhou County,Pingxiang City,Jingxi City and Napo County in Guangxi.[Results]There were 22 species of diseases and insect pests that harmed B.ramiflora in Guangxi,7 of which were diseases and 15 were insect pests.Leaf blight(Fuasrium spp.)was the main disease at seedling stage,causing severe damage,and anthracnose(Colletotrichum gloeosporioides Penz.)caused moderate damage.Cyclosia papilionaris Drury and Chrysochus chinensis Baly had high frequency of occurrence,wide damage area and severe damage.Cyclosia panthono Stoll and Lcerya aegyptiaca Douglas caused moderate damage,while other insect pests caused light damage.[Conclusions]The study lays a foundation for the scientific prevention and control of B.ramiflora diseases and insect pests,and promotes the sustainable and rapid development of B.ramiflora industry in Guangxi.

Effects of Raising Chickens Under Moringa oleifera Forests on Soil Nutrients and Selenium Absorption by M. oleifera

[Objectives]This study was conducted to investigate the effects of raising chickens under Moringa oleifera forests on soil nutrient changes and selenium absorption by M.oleifera.[Methods]The nutrient contents of the soil and the selenium contents of different parts of M.oleifera were determined and analyzed.[Results]Raising chickens under the investigated M.oleifera forest improved the soil.Compared with the control,the soil organic matter,hydrolyzable nitrogen,available phosphorus,and available potassium of the plots for underforest chicken raising were significantly improved;the changes were the most significant within the range of 2 m from the chicken houses,respectively,and the values were 1.39,1.38,1.52 and 1.31 times of the control,respectively;soil bulk density decreased,total porosity increased,soil structure and permeability improved,and soil pH values at 2 and 5 m away from the chicken houses were significantly higher than the control by 0.34 and 0.28 unit,respectively;and raising chickens under the forest promoted the absorption and utilization of selenium in M.oleifera,and the selenium contents in stems and leaves of M.oleifera were 1.64 times and 1.51 times of the control,respectively.[Conclusions]This study provides a theoretical basis for the high-value selenium-enriching compound economic model of raising chickens under M.oleifera forests.