Two different immunoassay methods, competitive indirect enzyme-linked immuno-sorbent assay (CI-ELISA) and amplificative competitive indirect ELISA (ACI-ELISA) using biotin-avidin complex system were studied to detect ...Two different immunoassay methods, competitive indirect enzyme-linked immuno-sorbent assay (CI-ELISA) and amplificative competitive indirect ELISA (ACI-ELISA) using biotin-avidin complex system were studied to detect rhEPO. The linear ranges were 50-20000 ng/mL and 10-50000 ng/mL for CI-ELISA and ACI-ELISA, respectively. The low detection limits of CI-ELISA and ACI-ELISA were 62.8 ng/mL and 8.5 ng/mL, respectively.展开更多
Polymer capable of specific binding to Cu-dipyridyl complex was prepared by molecular imprinting technology. The binding specificity of the polymer to the template (Cu-dipyridyl complex) was investigated by cyclic vo...Polymer capable of specific binding to Cu-dipyridyl complex was prepared by molecular imprinting technology. The binding specificity of the polymer to the template (Cu-dipyridyl complex) was investigated by cyclic voltametric scanning using the carbon paste electrode modified by polymer particles in phosphate buffer solution. Factors that influence rebinding of the imprinted polymer were explored. The result demonstrated that the cyclic voltammetry was an efficient approach to explore interactions between template and imprinted polymers.展开更多
s: From hops of Humulus lupulus, a novel prenylchalcone was isolated, which showed inhibition against BGC-823 cells. The structure was elucidated as 5'-(2''-hydroxyisopropyl-)- dihydrofurano-[2', 3'...s: From hops of Humulus lupulus, a novel prenylchalcone was isolated, which showed inhibition against BGC-823 cells. The structure was elucidated as 5'-(2''-hydroxyisopropyl-)- dihydrofurano-[2', 3'-B]-4, 4'-dihydroxy-6'-methoxychalcone, by HRFABMS and NMR spectra.展开更多
Thioester method was improved by using Pac (phenacyl group) ester as protecting group of 3-mercaptopropionic acid. Two cyclopentapeptides c(Ala-Tyr-Leu-Ala-Gly) and c(Pro-Tyr-Leu- Ala-Gly) were synthesized successful...Thioester method was improved by using Pac (phenacyl group) ester as protecting group of 3-mercaptopropionic acid. Two cyclopentapeptides c(Ala-Tyr-Leu-Ala-Gly) and c(Pro-Tyr-Leu- Ala-Gly) were synthesized successfully by this method.展开更多
A novel conjugate of dibenzoyldiazomethane and polyamide containing N-methylimida-zoles was synthesized by a chloroform reaction and a DCC/HOBT coupling reaction.
文摘Two different immunoassay methods, competitive indirect enzyme-linked immuno-sorbent assay (CI-ELISA) and amplificative competitive indirect ELISA (ACI-ELISA) using biotin-avidin complex system were studied to detect rhEPO. The linear ranges were 50-20000 ng/mL and 10-50000 ng/mL for CI-ELISA and ACI-ELISA, respectively. The low detection limits of CI-ELISA and ACI-ELISA were 62.8 ng/mL and 8.5 ng/mL, respectively.
基金supported by the National Nalural Science Foundation of China(29975001)
文摘Polymer capable of specific binding to Cu-dipyridyl complex was prepared by molecular imprinting technology. The binding specificity of the polymer to the template (Cu-dipyridyl complex) was investigated by cyclic voltametric scanning using the carbon paste electrode modified by polymer particles in phosphate buffer solution. Factors that influence rebinding of the imprinted polymer were explored. The result demonstrated that the cyclic voltammetry was an efficient approach to explore interactions between template and imprinted polymers.
文摘s: From hops of Humulus lupulus, a novel prenylchalcone was isolated, which showed inhibition against BGC-823 cells. The structure was elucidated as 5'-(2''-hydroxyisopropyl-)- dihydrofurano-[2', 3'-B]-4, 4'-dihydroxy-6'-methoxychalcone, by HRFABMS and NMR spectra.
文摘Thioester method was improved by using Pac (phenacyl group) ester as protecting group of 3-mercaptopropionic acid. Two cyclopentapeptides c(Ala-Tyr-Leu-Ala-Gly) and c(Pro-Tyr-Leu- Ala-Gly) were synthesized successfully by this method.
文摘A novel conjugate of dibenzoyldiazomethane and polyamide containing N-methylimida-zoles was synthesized by a chloroform reaction and a DCC/HOBT coupling reaction.
