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Epidemiology of sports injuries referring to Kashan University of Medical Sciences Trauma Research Center from 2005 to 2011
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作者 Mohammad Reza Sharif Ali Akbarnejad Alireza Moravveji Rasool Hamayattalab Mansour Sayyah 《Chinese Journal of Traumatology》 CAS CSCD 2014年第6期323-326,共4页
Objective: Among the injury types, sports ones constitute a considerable proportion of patients who refer to the medical centers. This research was conducted to examine the frequency of sports- related injuries refer... Objective: Among the injury types, sports ones constitute a considerable proportion of patients who refer to the medical centers. This research was conducted to examine the frequency of sports- related injuries referring to Kashan University of Medical Sciences Trauma Research Center from 2005 to 2011. Methods: This was a retrospective research in which existing data from the data bank of Kashan University of Medical Sciences Trauma Research Center were employed. The data were extracted from the main source by SPSS version 16.0. Variables such as age, education, occupation and gender were analyzed. Results: The highest proportion of injuries was observed in students (59.4%) followed by workers (11.8%). Upper and lower extremities were most commonly injured. The most frequent injury was strain (35.4%), followed by sprain (27.7%). Conclusion: The results of this research showed that the majority of the sports trauma occurrs in students; therefore, they need more attention in regard to sports injuries. Preventive measures such as informing the coaches and teachers as well as increasing the students' awareness about the injury risk can decrease the incidences of sports injuries. 展开更多
关键词 Athletic injuries EPIDEMIOLOGY Kashan
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急性胰腺炎的急诊救治新进展
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作者 陈三洋 朱长举 《中国急救医学》 CAS CSCD 2024年第10期903-907,共5页
急性胰腺炎(acute pancreatitis,AP)是引起急性腹痛的常见病因之一,其病情可分为轻症急性胰腺炎(MAP)、中重度急性胰腺炎(MSAP)及重症急性胰腺炎(SAP),其中SAP的发病率约为25%。SAP病情危重,常合并多脏器功能障碍,病死率高达30%。AP的... 急性胰腺炎(acute pancreatitis,AP)是引起急性腹痛的常见病因之一,其病情可分为轻症急性胰腺炎(MAP)、中重度急性胰腺炎(MSAP)及重症急性胰腺炎(SAP),其中SAP的发病率约为25%。SAP病情危重,常合并多脏器功能障碍,病死率高达30%。AP的病因在我国以胆源性、高脂性和酒精性居多,其发病机制主要包括胰蛋白酶原的异常激活、病理性钙离子(Ca 2+)信号、线粒体功能紊乱等。AP的治疗目前以补液、抗炎等内科综合治疗和内镜、穿刺等微创治疗为主。与传统手术治疗相比,近年来出现的如升阶梯治疗策略等新的治疗方法具有显著优势。因此,充分了解AP患者的相关危险因素及发病机制,应用不同方法对患者严重程度进行评估,以采取有效方式进行治疗,能明显改善患者预后。 展开更多
关键词 急性胰腺炎 病因 发病机制 治疗 外泌体 升阶梯治疗
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Predictors of Chronic Physical and Mental Quality of Life Following Traumatic Brain Injury
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作者 Shahrokh Yousefzade-Chabok Sara Ramezani Kapourchali +3 位作者 Zoheir Reihanian Ehsan Kazemnezhad Leili Anoush Dehnadi Moghadam Zahra Mohtasham Amiri 《Health》 2014年第6期496-503,共8页
Objective and Background: This study aimed at determining the predictors of chronic physical and mental quality of life (QOL) in patients with traumatic brain injury (TBI) focusing on neuropsychological functions post... Objective and Background: This study aimed at determining the predictors of chronic physical and mental quality of life (QOL) in patients with traumatic brain injury (TBI) focusing on neuropsychological functions post trauma. Materials and Methods: This is a longitudinal study in which 257 patients having inclusion criteria were enrolled. Neuropsychological tasks including logical memory, verbal paired associates, visual memory, verbal expression, auditory comprehension, semantic judgment and semantic categories were implemented. The appearance of psychiatric disorder, Agnosia, Apraxia, Dysarthria and pragmatic linguistic disorder post trauma were evaluated at discharge. QOL was studied 6 months after injury by filling SF-36 questionnaire via phone interview with patients. Results: Appearance of some post-traumatic disorders including agnosia, pragmatic linguistic disorder and psychiatric disorder were significantly correlated to poor QOL. The final step of logistic regression model showed that TBI severity, verbal memory, auditory comprehension and semantic acceptability scores were predictors of unfavorable mental QOL as well as TBI severity, injury severity scale (ISS) score and multifocal lesions for unfavorable physical QOL. Discussion: Thus, it is recommended that clinicians choose medical therapeutic priorities to improve the verbal neuropsychological sequela and provide preliminaries for a chronic favorable mental QOL. Furthermore, to prevent of chronic unfavorable physical QOL, early care of organic injuries should be considered especially in patients with severe and multifocal TBI. 展开更多
关键词 TRAUMATIC Brain Injury Physical and Mental Quality of LIFE Short Form-36 QUESTIONNAIRE NEUROPSYCHOLOGICAL Functions
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Incidence and pattern of traumatic spine injury in a single level I trauma center of southern Iran
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作者 Mahnaz Yadollahi Mehrdad Karajizadeh +1 位作者 Najmeh Bordbar Zahra Ghahramani 《Chinese Journal of Traumatology》 CAS CSCD 2023年第4期199-203,共5页
Purpose:Spine injury is one of the leading causes of death and mortality worldwide.The objective of this study was to determine the incidence,pattern and outcome of trauma patients with spine injury referred to the la... Purpose:Spine injury is one of the leading causes of death and mortality worldwide.The objective of this study was to determine the incidence,pattern and outcome of trauma patients with spine injury referred to the largest trauma center in southern Iran during the last 3 years.Methods:This is a cross-sectional study conducted between March 2018 and June 2021 in the largest trauma center in the southern Iran.The data collection form included the age,sex,injury location(cervical,thoracic,and lumbar),cause of injury(traffic accidents,falls,and assaults),length of hospital stay,injured segment of spine injury,severity of injury,and outcome.Statistical analyzes were performed using SPSS software version 24.Results:Totally 776 cases of spine injury were identified.The spine injury rate was 17.0%,and the mortality rate was 15.5%.Cervical spine injury(20.4%)more often occulted in motorcycle accident,and thoracic spine injury(20.1%)occulted in falls.The highest and lowest rates of spine injurys were related to lumbar spine injury(30.2%)and cervical spine injury(21.5%),respectively.There was a statistically significant relationship between the mechanism of injury and the location of spine injury(p<0.001).And patients with lumbar spine injury had the highest mortality rate(16.7%).Injury severity score(OR=1.041,p<0.001)and length of stay(OR=1.018,p<0.001)were strong predictors of mortality in trauma patients with spine injury.Conclusion:The results of the study showed that the incidence of traumatic spine injury rate was approximately 17.0%in southern of Iran.Road traffic injury and falls are the common mechanism of injury to spine.It is important to improve the safety of roads,and passengers,as well as work envi-ronment,and improve the quality of cars.Also,paying attention to the pattern of spine injury may assist to prevent the missing diagnosis of spine injury in multiple trauma patients. 展开更多
关键词 TRAUMA Spinal cord INJURY INCIDENCE PATTERN
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An analysis of association of personality traits with job burnout among nurses working in hospital
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作者 Hossein AKBARI Masoud MOTALEBI KASHANI +2 位作者 Sedighe DEHGHANI BIDGOLI Masoumeh Koochaki NASRABADI Fahimeh KARAMALI 《Journal of Integrative Nursing》 2023年第1期43-49,共7页
Objective:The objective of the present study is to explore the effects of personality traits on job burnout among hospital nurses.Materials and Methods:This cross-sectional research was done during 2019-2020 at Kashan... Objective:The objective of the present study is to explore the effects of personality traits on job burnout among hospital nurses.Materials and Methods:This cross-sectional research was done during 2019-2020 at Kashan Shahid Beheshti Hospital.The data analysis procedures included descriptive statistics and the partial least squares-based structural equation modeling.The participants were 150 nursing professionals.A questionnaire indicating information on demographics,burnout(measured using the Maslach Burnout Inventory with three dimensions of depersonalization,emotional exhaustion,and personal accomplishment),and personality profile(measured employing the neuroticism extraversion openness five-factor inventory including extroversion,conscientiousness,agreeableness,neuroticism,and openness to experience dimensions)was used to gather the required data.Results:The results of the study showed that the validity and reliability of the measurement model were desirable(factor load higher than 0.5,the Cronbach’s alpha value and the composite reliability are>0.7).Structural model showed statistically drastic,negative relationship between the nurses’burnout levels and neuroticism(β=0.722)and openness to experience(β=0.437).However,the relationship was significantly positive between the nurses’burnout levels and conscientiousness(β=0.672),agreement(β=0.594),and extraversion(β=0.559)(P<0.03).Conclusions:The present study helped the recognition of burnout among nurses working in hospitals and approved the effects of personality features on the burnout experience. 展开更多
关键词 Hospital nursing job burnout nurses personality traits professional burnout
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Post-discharge follow-up of patients with spine trauma in the National Spinal Cord Injury Registry of Iran during the COVID-19 pandemic:Challenges and lessons learned
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作者 Zahra Azadmanjir Moein Khormali +14 位作者 Mohsen Sadeghi-Naini Vali Baigi Habibollah Pirnejad Mohammad Dashtkoohi Zahra Ghodsi Seyed Behnam Jazayeri Aidin Shakeri Mahdi Mohammadzadeh Laleh Bagheri Mohammad-Sajad Lotfi Salman Daliri Amir Azarhomayoun Homayoun Sadeghi-Bazargani Gerard O'reilly Vafa Rahimi-Movaghai 《Chinese Journal of Traumatology》 CAS CSCD 2024年第3期173-179,共7页
Purpose:The purpose of the National Spinal Cord Injury Registry of Iran(NSCIR-IR)is to create an infrastructure to assess the quality of care for spine trauma and in this study,we aim to investigate whether the NSCIR-... Purpose:The purpose of the National Spinal Cord Injury Registry of Iran(NSCIR-IR)is to create an infrastructure to assess the quality of care for spine trauma and in this study,we aim to investigate whether the NSCIR-IR successfully provides necessary post-discharge follow-up data for these patients.Methods:An observational prospective study was conducted from April 11,2021 to April 22,2022 in 8 centers enrolled in NSCIR-IR,respectively Arak,Rasht,Urmia,Shahroud,Yazd,Kashan,Tabriz,and Tehran.Patients were classified into 3 groups based on their need for care resources,respectively:(1)non-spinal cord injury(SCI)patients without surgery(group 1),(2)non-SCI patients with surgery(group 2),and(3)SCI patients(group 3).The assessment tool was a self-designed questionnaire to evaluate the care quality in 3 phases:pre-hospital,in-hospital,and post-hospital.The data from the first 2 phases were collected through the registry.The post-hospital data were collected by conducting follow-up assessments.Telephone follow-ups were conducted for groups 1 and 2(non-SCI patients),while group 3(SCI patients)had a face-to-face visit.This study took place during the COVID-19 pandemic.Data on age and time interval from injury to follow-up were expressed as mean±standard deviation(SD)and response rate and follow-up loss as a percentage.Results:Altogether 1538 telephone follow-up records related to 1292 patients were registered in the NSCIR-IR.Of the total calls,918(71.05%)were related to successful follow-ups,but 38 cases died and thus were excluded from data analysis.In the end,post-hospital data from 880 patients alive were gathered.The success rate of follow-ups by telephone for groups 1 and 2 was 73.38%and 67.05%respectively,compared to 66.67%by face-to-face visits for group 3,which was very hard during the COVID-19 pandemic.The data completion rate after discharge ranged from 48%-100%,22%-100%and 29%-100%for groups 1-3.Conclusions:To improve patient accessibility,NSCIR-IR should take measures during data gathering to increase the accuracy of registered contact information.Regarding the loss to follow-ups of SCI patients,NSCIR-IR should find strategies for remote assessment or motivate them to participate in follow-ups through,for example,providing transportation facilities or financial support. 展开更多
关键词 Follow-up studies Spinal injuries Spinal cord injuries COVID-19 NSCIR-IR
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脓毒症免疫紊乱及其临床意义 被引量:81
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作者 吴田田 姚咏明 《解放军医学杂志》 CAS CSCD 北大核心 2017年第2期95-102,共8页
脓毒症主要由机体免疫功能紊乱导致,即由对感染、创伤等致病因素过度的炎症反应发展至免疫麻痹或抑制。细胞免疫应答在脓毒症发生、发展过程中发挥重要作用。在过度炎症期,诸多免疫细胞被病原相关分子模式(PAMPs)、损伤相关分子模式(DAM... 脓毒症主要由机体免疫功能紊乱导致,即由对感染、创伤等致病因素过度的炎症反应发展至免疫麻痹或抑制。细胞免疫应答在脓毒症发生、发展过程中发挥重要作用。在过度炎症期,诸多免疫细胞被病原相关分子模式(PAMPs)、损伤相关分子模式(DAMPs)激活并分泌大量促炎细胞因子;在免疫麻痹期,过度凋亡导致免疫细胞数量减少伴随功能下降,调节性免疫细胞亚群逐步占据优势并分泌抑制性细胞因子。监测脓毒症中机体免疫功能的变化及其转归是制定免疫调节治疗方案的基础及依据,但目前尚缺乏能应用于临床并综合评价整体免疫功能状态的指标体系。 展开更多
关键词 脓毒症 免疫紊乱 免疫 细胞 细胞凋亡 监测 免疫学
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烧伤创面脓毒症的防治对策 被引量:9
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作者 吴田田 童亚林 姚咏明 《创伤外科杂志》 2017年第5期396-400,共5页
烧创伤破坏皮肤连续性及完整性,影响机体免疫反应,甚至诱导免疫抑制。感染是重度烧伤患者常见、严重甚至致死的并发症。创面脓毒症是创面严重感染导致的全身性炎症反应,是烧伤患者主要死因之一。创面脓毒症的治疗重在预防。对创面及时... 烧创伤破坏皮肤连续性及完整性,影响机体免疫反应,甚至诱导免疫抑制。感染是重度烧伤患者常见、严重甚至致死的并发症。创面脓毒症是创面严重感染导致的全身性炎症反应,是烧伤患者主要死因之一。创面脓毒症的治疗重在预防。对创面及时、有效的处置是防治创面脓毒症的基础及关键。在传统抗感染治疗策略基础上,探索抗感染治疗的新方向、新制剂对于缓解抗菌药物耐药、改善创面脓毒症患者预后具有重要意义。针对创面脓毒症病原菌的免疫治疗亦具有广阔的临床应用前景。 展开更多
关键词 烧伤 创伤 创面脓毒症 预防 治疗
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自噬与炎症性疾病的相互作用与关系 被引量:2
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作者 王雪松 寿松涛 姚咏明 《解放军医学杂志》 CAS CSCD 北大核心 2019年第9期778-781,共4页
自噬是生物体维持细胞内环境稳态、进化上高度保守的重要的生理生化过程。自噬广泛参与多种疾病的发生发展过程,其与炎症性疾病的相互作用是近年研究的热点。自噬相关基因(ATGs)参与自噬的多个环节,对自噬发挥着重要的调节作用,也影响... 自噬是生物体维持细胞内环境稳态、进化上高度保守的重要的生理生化过程。自噬广泛参与多种疾病的发生发展过程,其与炎症性疾病的相互作用是近年研究的热点。自噬相关基因(ATGs)参与自噬的多个环节,对自噬发挥着重要的调节作用,也影响着炎症性疾病的进程和转归。该文综述自噬及其重要ATGs在炎症性疾病中的调节作用和机制,为研究炎症性疾病的发病机制和调控途径提供新的思路。 展开更多
关键词 自噬 炎症性疾病 自噬相关基因
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外科脓毒症免疫调控障碍机制 被引量:9
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作者 姚咏明 栾樱 《医学研究生学报》 CAS 北大核心 2019年第1期26-30,共5页
创伤、烧伤和出血引起的脓毒症是外科危重症患者死亡的主要原因,可能导致宿主天然免疫和获得性免疫反应的广泛激活和功能障碍。脓毒症时大量天然免疫细胞和获得性免疫细胞亚群,如中性粒细胞、巨噬细胞、树突状细胞、T淋巴细胞、调节性T... 创伤、烧伤和出血引起的脓毒症是外科危重症患者死亡的主要原因,可能导致宿主天然免疫和获得性免疫反应的广泛激活和功能障碍。脓毒症时大量天然免疫细胞和获得性免疫细胞亚群,如中性粒细胞、巨噬细胞、树突状细胞、T淋巴细胞、调节性T细胞和自然杀伤细胞,主要通过调节细胞表面受体表达或细胞因子分泌进而影响脓毒症的发展和结局。因此,深入了解脓毒症的免疫调控障碍机制,对判断脓毒症患者的预后,指导其并发症的治疗具有重要意义。 展开更多
关键词 脓毒症 免疫功能 调控机制
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线粒体自噬中受体蛋白FUNDC1研究进展 被引量:10
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作者 罗以楠(综述) 邱俏檬 +1 位作者 卢中秋 姚咏明(审校) 《医学研究生学报》 CAS 北大核心 2020年第5期537-542,共6页
线粒体是调节细胞能量平衡的关键细胞器,其在细胞代谢、应激反应和细胞死亡中具有重要意义。而在内外环境的影响下,受损或衰老的线粒体对细胞生存造成严重威胁。线粒体自噬是指细胞选择性消除功能失调的线粒体,从而维持细胞内环境稳定。... 线粒体是调节细胞能量平衡的关键细胞器,其在细胞代谢、应激反应和细胞死亡中具有重要意义。而在内外环境的影响下,受损或衰老的线粒体对细胞生存造成严重威胁。线粒体自噬是指细胞选择性消除功能失调的线粒体,从而维持细胞内环境稳定。FUNDC1是新近发现的一种线粒体自噬受体蛋白,在介导线粒体自噬方面发挥重要调节作用。文章主要就近年来线粒体自噬中FUNDC1调控机制及其病理生理意义的研究进展进行综述。 展开更多
关键词 线粒体 线粒体自噬 受体蛋白 FUNDC1 调控机制
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毒胡萝卜素诱导内质网应激对小鼠调节性T细胞免疫功能的影响 被引量:3
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作者 冯珍珍 刘萤 +3 位作者 董宁 于燕 马涛 姚咏明 《解放军医学杂志》 CAS CSCD 北大核心 2018年第6期459-464,共6页
目的探讨内质网应激(ERS)特异性诱导剂毒胡萝卜素(TG)体外刺激对小鼠调节性T细胞(Treg)免疫功能及ERS相关信号通路的影响。方法采用免疫磁珠分离法分选BALB/c小鼠脾脏Treg,流式细胞术检测其分选纯度。采用流式细胞术检测Treg叉头翼状螺... 目的探讨内质网应激(ERS)特异性诱导剂毒胡萝卜素(TG)体外刺激对小鼠调节性T细胞(Treg)免疫功能及ERS相关信号通路的影响。方法采用免疫磁珠分离法分选BALB/c小鼠脾脏Treg,流式细胞术检测其分选纯度。采用流式细胞术检测Treg叉头翼状螺旋转录因子(Foxp3)及细胞毒性T细胞相关抗原4(CTLA-4)表达的时间及剂量效应关系。Western blotting检测细胞ERS信号通路相关分子葡萄糖调节蛋白78(GRP78)、真核细胞转录起始因子2α(e IF2α)、磷酸化e IF2α(p-e IF2α)、转录激活因子4(ATF4)表达/活化水平。将Treg单独或与效应T细胞(Teff)共培养,采用流式细胞术检测Teff的增殖活性,ELISA法检测Treg白细胞介素(IL)-10和转化生长因子(TGF)-β生成以及共培养上清中IL-2、IL-4、干扰素(IFN)-γ水平。结果磁珠分选Treg的纯度为91.0%。依据Treg Foxp3及CTLA-4表达的时效-量效关系,选定TG 0.1μmol/L和12h为实验刺激浓度和时间。0.1μmol/L TG刺激12h后,Treg中ERS信号通路相关分子GRP78、磷酸化e IF2α、ATF4等表达/活化水平明显上调,差异均有统计学意义(P<0.05)。与对照组相比,TG刺激组Treg IL-10和TGF-β分泌量明显增加,其对Teff增殖活性抑制能力明显增强,与Teff共培养上清中IL-4/IFN-γ比值明显升高,IL-2水平明显下降,差异均有统计学意义(P<0.05)。结论 TG能够有效地活化Treg中ERS相关信号通路,进而显著增强Treg介导的免疫抑制反应。 展开更多
关键词 调节性T细胞 免疫功能 内质网应激 毒胡萝卜素
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皮肤驻留调节性T细胞在烧伤创面愈合中的作用 被引量:2
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作者 信跃文 董宁 +2 位作者 吴瑶 柴艳芬 姚咏明 《解放军医学杂志》 CAS CSCD 北大核心 2020年第6期582-586,共5页
目的探讨皮肤驻留调节性T细胞(Tregs)在烧伤小鼠创面愈合中的作用。方法选取叉头翼状螺旋转录因子p3(Foxp3)DTR型小鼠及野生型C57BL/6小鼠,分别设为Foxp3DTR型-白喉毒素注射组(F-DT组)和野生型-白喉毒素注射组(C-DT组),组内依据随机数... 目的探讨皮肤驻留调节性T细胞(Tregs)在烧伤小鼠创面愈合中的作用。方法选取叉头翼状螺旋转录因子p3(Foxp3)DTR型小鼠及野生型C57BL/6小鼠,分别设为Foxp3DTR型-白喉毒素注射组(F-DT组)和野生型-白喉毒素注射组(C-DT组),组内依据随机数字表法分为早期组(n=9)与晚期组(n=9)。早期组小鼠于烧伤早期(烧伤前1天、伤后第0天、伤后第3天)腹腔注射10 ng/g DT,晚期组于烧伤晚期(伤后第5、7、9天)注射。于不同时间点对小鼠创面拍照,并测量伤口面积以评估伤口愈合率;HE染色光镜下观察创面再上皮化情况;流式细胞术分析野生型小鼠伤后不同时间点皮肤Tregs局部浸润及功能表型的变化。结果早期耗竭Tregs后,F-DT组小鼠烧伤创面愈合率较C-DT组显著降低(P<0.05);晚期耗竭Tregs后,两组烧伤创面愈合率差异无统计学意义(P>0.05)。HE染色显示,与C-DT组相比,F-DT早期组伤口关闭缓慢,瘢痕生成及结痂进程较慢,肉芽组织增生明显,血管生成及再上皮化进程显著减慢。流式细胞术分析皮肤Tregs浸润情况发现,CD4+Foxp3+T细胞浸润烧伤后显著增加,5 d后达峰值,然后逐渐恢复至基础水平(P<0.05),CD25+Tregs烧伤后3 d达峰值,然后逐渐下降至基础水平(P<0.05)。进一步分析可见,皮肤Tregs的CTLA-4表达水平在烧伤后显著增高,第3天达峰值,然后逐渐降至基础水平(P<0.05);ICOS表达水平于伤后第5天达峰值,第11天降至基础水平(P<0.05)。结论烧伤早期皮肤Tregs浸润于创面局部,有助于促进烧伤创面的愈合。 展开更多
关键词 皮肤驻留调节性T细胞 烧伤创面 愈合过程 炎症反应 免疫调节
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组织工程支架材料对固有免疫反应的影响及调控效应 被引量:2
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作者 陈坤威 童亚林 姚咏明 《解放军医学杂志》 CAS CSCD 北大核心 2018年第8期698-703,共6页
组织工程生物材料支架作为促进机体组织损伤修复与再生的工具,在临床上已经使用多年。生物材料植入机体后不可避免地诱发宿主反应,过度的宿主反应会影响材料植入的成功率,直接影响损伤修复效果。如何调控宿主反应是组织修复过程中的关... 组织工程生物材料支架作为促进机体组织损伤修复与再生的工具,在临床上已经使用多年。生物材料植入机体后不可避免地诱发宿主反应,过度的宿主反应会影响材料植入的成功率,直接影响损伤修复效果。如何调控宿主反应是组织修复过程中的关键问题。材料诱发的宿主反应主要由机体免疫系统参与调控,特别是固有免疫系统,同时固有免疫反应也是组织修复与再生过程中不可或缺的重要因素。近年来,生物材料对免疫功能的影响日益受到关注,许多资料表明组织工程材料参与固有免疫调控过程,材料成分,表面化学、理化特性,以及降解产物等均不同程度地影响固有免疫细胞的功能。本文对固有免疫在组织修复中的作用以及组织工程支架材料特性对固有免疫反应的影响作一综述。 展开更多
关键词 组织工程 生物材料支架 生物材料特性 固有免疫 组织修复与再生
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高乳酸血症对脓毒症小鼠树突状细胞功能的影响及作用机制 被引量:3
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作者 刘奇 赵洪强 +2 位作者 姚人骐 姚咏明 李为民 《解放军医学院学报》 CAS 北大核心 2021年第4期430-437,共8页
背景树突状细胞(dendritic cell,DC)功能障碍是脓毒症发生发展的重要原因之一。乳酸作为无氧糖酵解重要中间代谢产物,对多种免疫细胞包括DC具有明显的抑制效应。目的探讨脓毒症小鼠高乳酸血症对DC功能的影响及作用机制。方法C57BL/6小... 背景树突状细胞(dendritic cell,DC)功能障碍是脓毒症发生发展的重要原因之一。乳酸作为无氧糖酵解重要中间代谢产物,对多种免疫细胞包括DC具有明显的抑制效应。目的探讨脓毒症小鼠高乳酸血症对DC功能的影响及作用机制。方法C57BL/6小鼠随机分为假伤组及脓毒症组。采用盲肠结扎穿孔术构建小鼠脓毒症模型,通过血气分析仪检测小鼠外周血乳酸浓度。抗小鼠CD11c+免疫磁珠分离脾树突状细胞,流式细胞仪检测DC表面分子CD80、CD86及主要组织相容性复合体-Ⅱ(major histocompatibility complex-Ⅱ,MHC-Ⅱ)表达,酶联免疫吸附试验(ELISA)检测白细胞介素-2(interleukin-2,IL-2)、IL-4、IL-12及γ干扰素(interferon-γ,IFN-γ)细胞因子表达;激光共聚焦显微镜观察自噬体的形成及数量。结果脓毒症组小鼠外周血乳酸浓度较假伤组明显升高,且在术后24 h及48 h较为显著[24 h:(2.61±0.54)mmol/L vs(1.43±0.58)mmol/L,P<0.01;48 h:(2.12±0.51)mmol/L vs(1.32±0.53)mmol/L,P<0.01]。脓毒症组小鼠DC表面分子CD80及MHC-Ⅱ表达在24 h时较假伤组明显升高(CD80:61.40%±9.20%vs 7.94%±1.01%,P<0.05;MHC-Ⅱ:79.91%±8.32%vs 30.34%±2.47%,P<0.05),并能有效刺激T细胞IFN-γ分泌,诱导T细胞向Th1极化,而脓毒症组小鼠48 h时DC功能出现明显抑制,并诱导T细胞向Th2极化(IFN-γ/IL-4:0.41±0.06 vs 1.00±0.00,P<0.01)。乳酸体外刺激可显著抑制DC功能,导致其表面分子表达及刺激T细胞分泌能力显著下调[脂多糖(lipopolysaccharide,LPS)组vs乳酸组。CD80:5.11%±0.52%vs 0.09%±0.02%,P<0.01;MHC-Ⅱ:96.89%±0.16%vs 95.96%±0.20%,P<0.05;CD86:67.26%±1.23%vs 32.45%±2.95%,P<0.01;IL-2:(3.12±1.25)pg/mL vs(0.44±0.14)pg/mL,P<0.05],LPS联合乳酸刺激DC呈现出相同的趋势,且与乳酸组差异无统计学意义(P>0.05)。脓毒症暴露和乳酸刺激均可诱导DC自噬过度活化,分别予以3-MA及Bafilomycin干预自噬均可有效逆转乳酸对DC功能的抑制效应。结论脓毒症小鼠高乳酸血症可能是DC功能紊乱的重要原因,乳酸可通过诱导过度细胞自噬的方式介导DC应答障碍。 展开更多
关键词 脓毒症 乳酸 免疫功能 树突状细胞 自噬
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生物材料对树突状细胞免疫功能影响及其调控途径 被引量:1
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作者 朱富军 童亚林 +1 位作者 盛志勇 姚咏明 《生理科学进展》 CAS CSCD 北大核心 2018年第6期411-417,共7页
树突状细胞(DC)是目前所知体内最强的专职抗原提呈细胞,系连接固有免疫与适应性免疫的主要桥梁。新近的研究提示,生物材料对DC免疫功能的效应是决定生物材料发挥作用的重要因素。本文重点介绍生物材料物理化学特性对DC免疫功能的影响及... 树突状细胞(DC)是目前所知体内最强的专职抗原提呈细胞,系连接固有免疫与适应性免疫的主要桥梁。新近的研究提示,生物材料对DC免疫功能的效应是决定生物材料发挥作用的重要因素。本文重点介绍生物材料物理化学特性对DC免疫功能的影响及其受体途径、信号通路,为深入理解生物材料与机体免疫系统的相互作用提供理论依据,并为生物材料的设计及选择开辟新途径。 展开更多
关键词 生物材料 树突状细胞 免疫功能 受体 信号通路
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肿瘤坏死因子α诱导蛋白8的生物学效应及其临床价值 被引量:1
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作者 张雷 栾樱译 姚咏明 《生理科学进展》 CAS CSCD 北大核心 2017年第6期417-423,共7页
肿瘤坏死因子α诱导蛋白8(tumor necrosis factor alpha induced protein-8,TNFAIP8)作为TNFAIP8家族的成员之一,被认为是一种具有抗凋亡和促肿瘤效应的蛋白分子,在细胞的存活和死亡中发挥重要作用。本文重点介绍TNFAIP8的发现过程、结... 肿瘤坏死因子α诱导蛋白8(tumor necrosis factor alpha induced protein-8,TNFAIP8)作为TNFAIP8家族的成员之一,被认为是一种具有抗凋亡和促肿瘤效应的蛋白分子,在细胞的存活和死亡中发挥重要作用。本文重点介绍TNFAIP8的发现过程、结构功能、生物学效应及其临床意义,并对其可能的作用机制进行概述。 展开更多
关键词 肿瘤坏死因子α诱导蛋白8 炎症 肿瘤 凋亡 自噬 免疫应答
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海藻酸钠/乙二醇壳聚糖双网络水凝胶对小鼠调节性T细胞免疫功能的影响 被引量:1
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作者 陈坤威 姚咏明 《解放军医学杂志》 CAS CSCD 北大核心 2019年第2期91-97,共7页
目的探讨海藻酸钠(Alg)/乙二醇壳聚糖(GC)双网络凝胶材料体外刺激对小鼠调节性T细胞(Treg)免疫功能的影响。方法制备Alg、GC单网络水凝胶材料及双网络(DN)水凝胶材料(Alg/GC),根据不同水凝胶材料将细胞分为Alg组、GC组、DN组及对照组。... 目的探讨海藻酸钠(Alg)/乙二醇壳聚糖(GC)双网络凝胶材料体外刺激对小鼠调节性T细胞(Treg)免疫功能的影响。方法制备Alg、GC单网络水凝胶材料及双网络(DN)水凝胶材料(Alg/GC),根据不同水凝胶材料将细胞分为Alg组、GC组、DN组及对照组。采用免疫磁珠分离法分选BALB/c小鼠脾脏CD4+CD25+Treg,应用流式细胞术分析Treg叉头翼状螺旋转录因子(Foxp3)及细胞毒性T细胞相关抗原(CTLA-4)在不同时效(刺激24h或48h)、不同培养环境[有无多黏菌素B(PMB)]下的表达水平,以确定后续细胞培养时间以及是否选用含PMB的培养基;观察Treg与效应T细胞共培养后T细胞增殖活性改变;采用酶联免疫吸附分析(ELISA)技术检测Treg分泌白细胞介素(IL)-10、转化生长因子(TGF)-β及效应T细胞分泌IL-2、IL-4与干扰素(IFN)-γ的水平。结果选定实验条件为刺激24h,并使用含PMB的培养基。Alg/GC双网络水凝胶及GC单网络水凝胶作用24h后,与对照组[CTLA-4 (47.73±5.35)%,TGF-β(558.75±48.58)pg/ml]比较,CD4+CD25+Treg中CTL A-4的表达水平[DN组(92.21±2.97)%,GC组(95.99±2.79)%]明显升高,TGF-β分泌量[DN组(905.03±73.04)pg/ml,GC组(957.06±138.70)pg/ml]明显增加,差异均有统计学意义(P<0.05),但各刺激组Foxp3表达水平差异无统计学意义(P>0.05)。Alg/GC双网络水凝胶及GC单网络水凝胶刺激后,与对照组[(42.04±1.35)%]相比,Treg对效应T细胞增殖活性的抑制作用[DN组(37.22±1.39)%,GC组(35.20±2.03)%]均明显增强,共培养后效应T细胞分泌IL-4/IFN-γ比值[DN组(2.30±0.22),GC组(2.57±0.23)]明显升高,与对照组(1.45±0.27)比较差异有统计学意义(P<0.05)。结论 Alg/GC双网络材料可显著增强Treg介导的免疫抑制功能,其成分中单网络材料GC水凝胶可能发挥了主要作用。 展开更多
关键词 调节性T细胞 海藻酸钠 乙二醇壳聚糖 双网络水凝胶 免疫调控 组织工程 生物材料
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What factors affect length of hospital stay among trauma patients? A single-center study, Southwestern Iran 被引量:6
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作者 Ali Kashkooe Mahnaz Yadollahi Forough Pazhuheian 《Chinese Journal of Traumatology》 CAS CSCD 2020年第3期176-180,共5页
Purpose: Trauma is a major health concern. Length of hospital stay (LOS) has been targeted as an important metric to assess trauma care. This study aims to evaluate the risk factors that affect LOS among trauma patien... Purpose: Trauma is a major health concern. Length of hospital stay (LOS) has been targeted as an important metric to assess trauma care. This study aims to evaluate the risk factors that affect LOS among trauma patients in a trauma center in Southwestern Iran.Methods: This cross-sectional study was conducted on patients admitted to Rajaee Trauma Center, Shiraz, Iran between January 1, 2018 and December 30, 2018. The inclusion criteria were age above 15 years and having traffic accident injuries, including car, motorcycle and pedestrian injury mechanisms. The exclusion criteria were existing diseases including cardiovascular, cerebral, renal, and pulmonary diseases prior to this study, dead upon arrival or within 48 h after admission, and stay at the hospital for less than 6 h. The risk variables analyzed for prolonged LOS were age, gender, mechanism of traffic accident injury, infection during hospital stay, type of injury, injury severity score, surgery during hospitalization, and survival. Poisson regression was performed to evaluate the partial effects of each co- variate on trauma hospitalization (≥3 days as longer stay).Results: This study was conducted on 14,054 patients with traffic accident injury and the mean age was (33.89 ± 15.78) years. Additionally, 74.35% of the patients were male, with male to female ratio of 2.90. The result of Poisson regression indicated that male patients, higher age, combination of thoracic injuries, onset of infected sites, and surgery patients were more susceptible to have a longer LOS. Considering the site of injury, patients with face injuries followed by those with thorax injuries had the highest means of LOS (3.74 days and 3.36 days, respectively). Simultaneous existence of surgical inter- vention and infection in a patient had the greatest impact on prolonged LOS.Conclusion: This study identified that age, gender, mechanism of injury, infection, type of injury, survival, and ISS could lead to prolongation of LOS, but the affect can be reduced by eliminating modifiable risk factors. 展开更多
关键词 Length of stay TRAUMA Accidents traffic Risk factors
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A study of mortal让y risk factors among trauma referrals to trauma center, Shiraz, Iran, 2017 被引量:2
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作者 Mahnaz Yadollahi 《Chinese Journal of Traumatology》 CAS CSCD 2019年第4期212-218,共7页
Purpose: Trauma is well known as one of the main causes of death and disability throughout the world. Identifying the risk factors for mortality in trauma patients can significantly improve the quality of care and pat... Purpose: Trauma is well known as one of the main causes of death and disability throughout the world. Identifying the risk factors for mortality in trauma patients can significantly improve the quality of care and patient outcomes, as well as reducing mortality rates. Methods:【n this retrospective cohort study, systematic randomization was used to select 849 patients referred to the main trauma center of south of Iran during a period of six months (February 2017-July 2017);the *patients case files were evaluated in terms of demographic information, pre- and post-accident conditions, clinical conditions at the time of admission and finally, accident outcomes. A logistic regression model was used to analyze the role of factors affecting mortality among subjects. Results: Among subjects, 60.4% were in the age-group of 15-39 years. There was a 10.4% mortality rate among patients and motor-vehicle accide nts were the most comm on mecha nism of injury (66.7%). Aging led to in creased risk of fatality in this study. For each urHt increase in Glasgow coma scale (GCS), risk of death decreased by about 40%(odds ratio (OR)= 0.63, 95% confidence interval (C/): 0.59—0.67). For each unit increase in injury severe score (ISS), risk of death increased by 10%(OR = 1.11%, 95% Cl: 1.08-1.14) and for each unit in crease in trauma revised injury severity score (TRISS), there was 18% decrease in the risk of fatality (OR = 0.82, 95% CI: 0.71-0.88). Conclusion: The most common cause of trauma and the most common cause of death from trauma was traffic accidents. It was also found that an increase in the ISS index increases the risk of death in trauma patients, but the increase in GCS, revised trauma score (RTS) and TRISS indices reduces the risk of death in trauma patients. The TRISS indicator is better predictor of traumatic death than other indicators. 展开更多
关键词 Risk factor FETAL MORTALITY WOUNDS and INJURIES
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