Relevance of proteolysis and proteasome activation in fatty liver graft preservation: An Institut Georges Lopez-1 vs University of Wisconsin appraisal

To compare liver proteolysis and proteasome activation in steatotic liver grafts conserved in University of Wisconsin (UW) and Institut Georges Lopez-1 (IGL-1) solutions.METHODSFatty liver grafts from male obese Zücker rats were conserved in UW and IGL-1 solutions for 24 h at 4 °Cand subjected to “ex vivo” normo-thermic perfusion (2 h; 37 °C). Liver proteolysis in tissue specimens and perfusate was measured by reverse-phase high performance liquid chromatography. Total free amino acid release was correlated with the activation of the ubiquitin proteasome system (UPS: measured as chymotryptic-like activity and 20S and 19S proteasome), the prevention of liver injury (transaminases), mitochondrial injury (confocal microscopy) and inflammation markers (TNF 1 alpha, high mobility group box-1 (HGMB-1) and PPAR gamma), and liver apoptosis (TUNEL assay, cytochrome c and caspase 3).RESULTSProfiles of free AA (alanine, proline, leucine, isoleucine, methionine, lysine, ornithine, and threonine, among others) were similar for tissue and reperfusion effluent. In all cases, the IGL-1 solution showed a significantly higher prevention of proteolysis than UW (P < 0.05) after cold ischemia reperfusion. Livers conserved in IGL-1 presented more effective prevention of ATP-breakdown and more inhibition of UPS activity (measured as chymotryptic-like activity). In addition, the prevention of liver proteolysis and UPS activation correlated with the prevention of liver injury (AST/ALT) and mitochondrial damage (revealed by confocal microscopy findings) as well as with the prevention of inflammatory markers (TNF1alpha and HMGB) after reperfusion. In addition, the liver grafts preserved in IGL-1 showed a significant decrease in liver apoptosis, as shown by TUNEL assay and the reduction of cytochrome c, caspase 3 and P62 levels.CONCLUSIONOur comparison of these two preservation solutions suggests that IGL-1 helps to prevent ATP breakdown more effectively than UW and subsequently achieves a higher UPS inhibition and reduced liver proteolysis.

Ergonomic Evaluation of the Perceived Mental Workload among Tunisian University Hospital Caregivers

Objectives: To evaluate the perceived mental workload and to identify its individual and professional determinants among Tunisian caregivers in university hospitals. Methods: A cross-sectional study was carried out in two university hospitals of the center of Tunisia, which employ 1179 paramedics providing care activities. The survey was conducted within a representative sample, of 301 caregivers assigned to 28 medical and surgical services, randomly drawn, after being paired according to age, gender and work schedule. It included structured inquiry (socio-professional characteristics, occupational perceived workload) and validated questionnaires (Job Content Questionnaire of Karasek, SF12, WAI). Results: Mental workload was perceived as “moderate” by 145 caregivers (48.49%) and as “heavy” by 119 caregivers (40.61% of them). Among the sample, 209 paramedics endured at least once the fixed night work schedules along their respective careers, with an average of 5.63 ± 7.54 years. According to the WAI index, 39.59% of subjects considered their capacity of work as low. Moreover, job strain was noted in 19.79%. Indeed, work latitude was unsatisfactory among 41% of caregiver, 60.1% of them mentioned the absence of colleagues’ support. A feeling of job insecurity was reported by 73.72% of subjects. Multi-varied analytic study concluded that heavier perceived mental work load was correlated to individual determinants particularly age >45 years (p -3), overweight or obesity (p = 0.02), more than three depending children (p -3), lower degree obtained (p -3), working grade as nurse assistant (P = 0.001), lower quality of physical life (0.010) and expressed willingness to retire early (p = 0.003). Conclusion: The heavy perception of mental workload associated to care activities, is one of the reasons of premature departure among nurses from professional activity. Preventive actions on professional determinants of perceived workload can reduce this phenomenon.

Methanolic extract of Ephedra alata inhibits breast cancer cells in vitro and in vivo

Objective:To determine the anticancer potential of the methanolic extract from Ephedra alata against breast cancer both in vitro and in vivo.Methods:The effects of the methanolic extract of Ephedra alata on the viability,migration as well as apoptosis of breast cancer 4T1 cells were measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay,Transwell assay,and annexin V-FITC staining assay,respectively.Histological examination was also carried out.Moreover,a murine breast cancer model was established to evaluate the inhibitory effect of the extract.Biochemical parameters including hepatic and non-hepatic enzymes,malondialdehyde,and glutathione were investigated.Results:The methanolic extract of Ephedra alata showed a strong anti-proliferative and anti-migratory activity against 4T1 cells in a dose-dependent manner.It also induced apoptosis in 4T1 cells.In an in vivo mouse model,the extract markedly inhibited tumor growth,reduced malondialdehyde,and hepatic and non-hepatic enzymes as well as increased glutathione level.Conclusions:The methanolic extract of Ephedra alata inhibits breast cancer in vitro and in vivo,which may be a promising anticancer agent.

Optimization and Characterization of Combined Degumming Process of Typha angustata L. Stem Fibers

Plant derived natural fibers have been widely investigated as alternatives to synthetic fibers in reinforcing polymers.Researchers over the years have explored many plant fibers using different extraction processes to study their physical,chemical,and mechanical properties.In this context,the present study relates to the extraction,characterization,and optimization of Typha angustata L.stem fibers.For this purpose,desirability functions and response surface methodology were applied to simultaneously optimize the diameter(D),linear density(LD);yield(Y),lignin fraction(L),and tenacity(T)of Typha stem fibers.Typha stems have been subjected to both alkali(NaOH)and enzymatic(pectinex ultra-SPL)treatments.Three levels of process variables including enzyme concentration(10,15,and 20 ml/L)and treatment duration(10,15,and 20 days)were used to design the experiments according to the factorial design.Experimental results were examined by analysis of variance and fitted to second order polynomial model using multiple regression analysis.The Derringer’s desirability function released that the values of process variables generating optimized diameter,linear density,yield,lignin ratio and tenacity are 20 ml/L and 20 days for concentration of pectinex ultra-SPL enzyme and treatment duration,respectively.Confirmation was performed and high degree of correlation was found between the experimental and statistical values.Moreover,the morphological structure has been investigated by the scanning electron microscope,showing a crenelated structure of ultimate fiber bundles of cellulose composing the Typha fiber.Compared to Typha stem non-treated fibers(TSNTF),Typha stem combined treated fibers(TSCTF),brings to improve mechanical properties.This change in mechanical properties is affected by modifying the fiber structure showing alpha cellulose of(66.86%)and lignin ratio of(10.83%)with a crystallinity index of(58.47%).

Improving the Effectiveness of Image Classification Structural Methods by Compressing the Description According to the Information Content Criterion

The research aims to improve the performance of image recognition methods based on a description in the form of a set of keypoint descriptors.The main focus is on increasing the speed of establishing the relevance of object and etalon descriptions while maintaining the required level of classification efficiency.The class to be recognized is represented by an infinite set of images obtained from the etalon by applying arbitrary geometric transformations.It is proposed to reduce the descriptions for the etalon database by selecting the most significant descriptor components according to the information content criterion.The informativeness of an etalon descriptor is estimated by the difference of the closest distances to its own and other descriptions.The developed method determines the relevance of the full description of the recognized object with the reduced description of the etalons.Several practical models of the classifier with different options for establishing the correspondence between object descriptors and etalons are considered.The results of the experimental modeling of the proposed methods for a database including images of museum jewelry are presented.The test sample is formed as a set of images from the etalon database and out of the database with the application of geometric transformations of scale and rotation in the field of view.The practical problems of determining the threshold for the number of votes,based on which a classification decision is made,have been researched.Modeling has revealed the practical possibility of tenfold reducing descriptions with full preservation of classification accuracy.Reducing the descriptions by twenty times in the experiment leads to slightly decreased accuracy.The speed of the analysis increases in proportion to the degree of reduction.The use of reduction by the informativeness criterion confirmed the possibility of obtaining the most significant subset of features for classification,which guarantees a decent level of accuracy.

Genetic investigation of the ubiquitin-protein ligase E3A gene as putative target in Angelman syndrome

BACKGROUND Angelman syndrome(AS)is caused by maternal chromosomal deletions,imprinting defects,paternal uniparental disomy involving chromosome 15 and the ubiquitin-protein ligase UBE3A gene mutations.However the genetic basis remains unclear for several patients.AIM To investigate the involvement of UBE3A gene in AS and identifying new potential genes using exome sequencing.METHODS We established a cohort study in 50 patients referred to Farhat Hached University Hospital between 2006 and 2021,with a strong suspicion of AS and absence of chromosomal aberrations.The UBE3A gene was screened for mutation detection.Two unrelated patients issued from consanguineous families were subjected to exome analysis.RESULTS We describe seven UBE3A variants among them 3 none previously described including intronic variants c.2220+14T>C(intron14),c.2507+43T>A(Exon15)and insertion in Exon7:c.30-47_30-46.The exome sequencing revealed 22 potential genes that could be involved in AS-like syndromes that should be investigated further.CONCLUSION Screening for UBE3A mutations in AS patients has been proven to be useful to confirm the diagnosis.Our exome findings could rise to new potential alternative target genes for genetic counseling.

Profiles of the Headspace Volatile Organic and Essential Oil Compounds from the Tunisian Cardaria draba (L.) Desv. and Its Leaf and Stem Epidermal Micromorphology

In this work, we investigated aroma volatiles emanated by dry roots, stems, leaves, flowers, and fruits of Cardariadraba (L.) Desv. growing wild in Tunisia and its aerial part essential oils (EOs) composition. A total of 37 volatileorganic compounds (96.7%–98.9%) were identified;4 esters, 4 alcohols, 7 hydrocarbons, 12 aldehydes, 5 ketones,1 lactone, 1 organosulfur compound, 2 organonitrogen compounds, and 1 acid. The hydrocarbons form the maingroup, representing 49.5%–84.6% of the total detected volatiles. The main constituent was 2,2,4,6,6-pentamethylheptane(44.5%–76.2%) reaching the highest relative percentages. Forty-two compounds were determined in thetwo fractions of EOs, representing 98.8% and 97.2% of the total oil composition, respectively. The principal componentswere hexadecanoic acid (34.6%), 6-methyl-5-hepten-2-one (18.3%), decanal (15.0%), 6,10,14-trimethyl-2-pentadecanone (13.2%), and n-pentacosane (13%). Micromorphological details of the leaf and stem epidermisusing light microscopy revealed polygonal cells with sinuous walls in the adaxial and abaxial leaf surfaces andnearly rectangular and long ones with linear and thick walls for the stem epidermis. The stomata complexes wereanisocytic in the leaf epidermis and mainly anisocytic and rarely paracytic in the stem epidermis. Non-glandulartrichomes were unbranched and long with an acute apex or short with a convex apex. The glandular ones wereidentified for the first time in this species. They were short-stalked with a large secretory head. The highest stomatalindex (17.02%) was recorded in the abaxial leaf surface. The identification of headspace volatiles and essentialoil compounds can be used to characterize this species, and the various epidermis micromorphologicalfeatures are very useful for biosystematics taxonomic studies within Brassicaceae.

Numerical Investigation of Heat and Mass Transfer in Nanofluid-Filled Porous Medium

In this work, we numerically study the laminar mixed convection of fluid flow in a vertical channel filled with porous media during the drying process. The porous medium, modeled as a vertical wall, consists of solid and nanofluid phase (Water-Al2O3 or Water-Cu), as well as a gas phase. The established model is developed based on Whitaker’s theory and resolved by our numerical code using Fortran. Results principally show the influence of various physical parameters, such as nanoparticle volume fraction, ambient temperature, and saturation on heat and mass transfer on the drying process. This study brings the effect of the presence of nanofluids in porous media. It contributes not only to our fundamental understanding of drying processes but also provides practical insights that can guide the development of more efficient and sustainable drying technologies. .

A Glassy Carbon Electrode Modified with Cellulose Nanofibrils from Ammophila arenaria for the Sensitive Detection of L-Trytophan

L-tryptophan is an essential amino acid for human health. Nanofibrillated cellulose (NFC) from marram grass (Ammophila arenaria) extracted from plants harvested in the center of Tunisia was used for the first time for the modification of a glassy carbon electrode (GCE), for the sensitive detection of L-tryptophan (Trp). After spectroscopic and morphological characterization of the extracted NFC, the GC electrode modification was monitored through cyclic voltammetry. The NFC-modified electrode exhibited good analytical performance in detecting Trp with a wide linear range between 7.5 × 10−4 mM and 10−2 mM, a detection limit of 0.2 µM, and a high sensitivity of 140.0 µA∙mM−1. Additionally, the NFC/GCE showed a good reproducibility, good selectivity versus other amino acids, uric acid, ascorbic acid, and good applicability to the detection of Trp in urine samples.

FlowBreakdown of Hybrid Nanofluid on a Rigid Surface with Power Law Fluid as Lubricated Layers

Thiswork investigates an oblique stagnation point flowof hybrid nanofluid over a rigid surface with power lawfluidas lubricated layers. Copper (Cu) and Silver (Ag) solid particles are used as hybrid particles acting in water H2O asa base fluid. The mathematical formulation of flow configuration is presented in terms of differential systemthat isnonlinear in nature. The thermal aspects of the flow field are also investigated by assuming the surface is a heatedsurface with a constant temperature T. Numerical solutions to the governing mathematical model are calculatedby the RK45 algorithm. The results based on the numerical solution against various flow and thermal controllingparameters are presented in terms of line graphs. The specific results depict that the heat flux increases over thelubricated-indexed parameter.

Effects of Institut Georges Lopez-1 and Celsior preservation solutions on liver graft injury

AIM: To compare Institut Georges Lopez(IGL-1) and Celsior preservation solutions for hepatic endothelium relaxation and liver cold ischemia reperfusion injury(IRI).METHODS: Two experimental models were used.In the first one, acetylcholine-induced endotheliumdependent relaxation(EDR) was measured in isolated ring preparations of rat hepatic arteries preserved or not in IGL-1 or Celsior solutions(24 h at 4 ℃).To determine nitric oxide(NO) and cyclooxygenase EDR, hepatic arteries were incubated with L-NG-nitroarginine methyl ester(L-NAME), an inhibitor of endothelium nitric oxide synthase(e NOS), or with L-NAME plus indomethacin, an inhibitor of cyclooxygenase.In the second experiment, rat livers were cold-stored in IGL-1 or Celsior solutions for 24 h at 4 ℃ and then perfused "ex vivo " for 2 h at 37 ℃.Liver injury was assessed by transaminase measurements, liver function by bile production and bromosulfophthalein clearance, oxidative stress by malondialdehyde levels and catalase activity and alterations in cell signaling pathways by pA kt, pA MPK, eN OS and MAPKs proteins level.RESULTS: After cold storage for 24 h with either Celsior or IGL-1, EDR was only slightly altered.Infreshly isolated arteries, EDR was exclusively mediated by NO.However, cold-stored arteries showed NOand COX-dependent relaxation.The decrease in NO-dependent relaxation after cold storage was significantly more marked with Celsior.The second study indicated that IGL-1 solution obtained better liver preservation and protection against IRI than Celsior.Liver injury was reduced, function was improved and there was less oxidative stress.IGL-1 solution activated Akt and AMPK, which was concomitant with increased eN OS expression and nitrite/nitrate levels.Furthermore, MAPKs kinases were regulated in livers preserved with IGL-1 solution since reductions in p-p38, p-ERK and p-JNK protein levels were observed.CONCLUSION: IGL-1 solution preserved NO-dependent relaxation better than Celsior storage solution and enhanced liver graft preservation.

Hypoxia inducible factor-1αaccumulation in steatotic liver preservation:Role of nitric oxide

AIM:To examine the relevance of hypoxia inducible factor(HIF-1)and nitric oxide(NO)on the preservation of fatty liver against cold ischemia-reperfusion injury(IRI). METHODS:We used an isolated perfused rat liver model and we evaluated HIF-1αin steatotic and non-steatotic livers preserved for 24 h at 4℃in University of Wisconsin and IGL-1 solutions,and then subjected to 2 h of normothermic reperfusion.After normoxic reperfusion,liver enzymes,bile production,bromosulfophthalein clearance,as well as HIF-1αand NO[endothelial NO synthase(eNOS)activity and nitrites/nitrates]were also measured.Other factors associated with the higher susceptibility of steatotic livers to IRI,such as mitochondrial damage and vascular resistance were evaluated. RESULTS:A significant increase in HIF-1αwas found in steatotic and non-steatotic livers preserved in IGL-1 after cold storage.Livers preserved in IGL-1 showed a significant attenuation of liver injury and improvement in liver function parameters.These benefits were enhanced by the addition of trimetazidine(an antiischemic drug),which induces NO and eNOS activation, to IGL-1 solution.In normoxic reperfusion,the presence of NO favors HIF-1αaccumulation,promoting also the activation of other cytoprotective genes,such as hemeoxygenase-1. CONCLUSION:We found evidence for the role of the HIF-1α/NO system in fatty liver preservation,especially when IGL-1 solution is used.

Relationship between high sensitivity C-reactive protein and angiographic severity of coronary artery disease

Background Coronary artery disease(CAD)remains a leading cause of morbidity and mortality.Cytokines play a potential role in atherosclerosis pathogenesis and progression.We investigated the association between high sensitive C-reactive protein(hs CRP)and severity of CAD.Methods CAD patients were stratified according to hs CRP cut-off value into high levels hs CRP group(≥8.4 mg/L)and low levels hs CRP group(<8.4 mg/L).Severity of CAD was assessed according to artery stenosis degree and the number of vessel involved.Statistical analysis was performed using Statistical Package for the Social Sciences(SPSS,version 23.0).Results The mean age was 60.3±11.0 years.The level of hs CRP was increased and ranged from 0.2 to 1020.0 mg/L.Biochemical risk factors and severity of CAD didn’t show significant differences between the two groups.In multivariate linear analysis,cardiac troponin I(c Tn I)and serum amyloid A(SAA)were predictors of hs CRP.As shown in receiver operating characteristic(ROC)curve analysis performed in patients with ST-segment elevation myocardial infarction(STEMI)and compared to myonecrosis biomarkers,hs CRP(area under the curve(AUC):0.905;95%CI:0.844-0.966;P<0.001)could be a powerful predictor marker in evaluating the infarct size after myocardial infarction but not better than c Tn I.Conclusions Hs CRP levels were not associated with the severity of CAD but could be useful in the evaluation of myocardial necrosis in patients with STEMI.

Genetic Diversity and Structure of Tunisian Local Pear Germplasm as Revealed by SSR Markers

Growing pear has a long tradition in Tunisia, and numerous local cultivars possessing an excellent adaptability and resilience potential to climatic variation are present. This large adaptability is associated with an important genetic diversity, which is threatened to erosion.Appropriate measures have to be taken in order to properly evaluate and conserve this local material. Microsatellite markers were used to assess the level of genetic diversity among Tunisian pear germplasm, and compare it with some European varieties and wild pear species. 61 pear accessions representing eight groups(six groups from Tunisia, one from Northern Europe and another group composed of wild pear) have been genotyped using SSR markers derived from apple and pear. The pear accessions showed a significant polymorphism and 95 polymorphic alleles were found. The number of alleles per locus varied from 5 for CH04e03 locus to 14 for CH01d09 locus with an average of 9.4 alleles per locus.Moreover, the mean gene diversity(H_e) per locus ranged from 0.192 to 0.752. Genetic distance values and cluster analyses revealed high genetic similarities among the Tunisian groups. Factorial correspondence analysis(FCA) categorized the accessions into three independent groups where Tunisian local accessions agglomerated together distantly from European and wild pear accessions. Additionally, UPGMA dendrogram grouped accessions into two clusters, confirmed thereafter by the Bayesian model-based Structure analysis. The results showed 16 putative triploid accessions found in the local germplasm. This study provides valuable information to develop strategies of local pear conservation and use.

CFD investigation of effect of nanofluid filled Trombe wall on 3D convective heat transfer

A numerical investigation was carried out on the effect of carbon nanotube(CNT)-water-nanofluid-filled Trombe wall on heat transfer and fluid flow inside a 3 D typical room.Time depending governing equations are considered with applying hot temperature at the left surface(collector) of the Trombe wall.The left wall(glazing) of the room and a square part(window) at the right wall are considered at cold temperature.The effects of Rayleigh number and the nanofluid volume fractions and the Trombe wall height on the temperature field,flow structure and heat transfer rate,are studied.The results show that the addition of nanoparticles and the increase of the Trombe wall height,enhance the heat transfer considerably and affect the flow structure and the temperature field.

Application of Prunus amygdalus By-products in Eco-friendly Dyeing of Textile Fabrics

Natural dyes have become an interesting subject of study because of their better ecological properties in comparison to their synthetic counterparts.This article concerns the dyeing of wool,silk,and polyamide fabrics with natural dyes extracted from almond shells and stems.The developed method of dyeing by these extracts is interesting and very attractive for several reasons:firstly,the extracts used are the black liquor discharged from the industries of delignification,which is a chemical process for removing lignin from agricultural wastes to produce a cellulosic fiber;(ii)these natural dyes are renewable and available in large quantities;(iii)this method is economical;and(iv)lastly,the dyeing performances of the obtained dyed textiles are very promising.The color of each dyed material was investigated in terms of the CIELAB coordinates and their fastness properties measured by washing,rubbing,and light.

Heat and mass transfer enhancement in triangular pyramid solar still using CNT-water nanofluid

This work represents a 3 D numerical study of the effects of carbon nanotube(CNT)-water nanofluids on the double diffusive convection inside the triangular pyramid solar still.This numerical investigation is performed for wide ranges of governing parameters such as buoyancy ratio(-10≤N≤0),volumetric fraction of nanoparticles(0≤Φ≤0.05) and Rayleigh number(10^(3)≤Ra≤10^(5)).The results are presented in terms of flow structure,temperature field,heat and mass transfer rates variations.It was found that the buoyancy ratio can be considered as an optimizing parameter for the heat and mass transfer,and the use of CNT has a positive effect on the solar still performances.

A Challenging Approach to Improve the Low Affinity of Acrylic Fibres to Be Successfully Dyed with a Bio-Colorant Extracted from Grape Marc

Acrylic fibres are highly crystalline and non-polar polymers,which makes their dyeing a very difficult step that poses real technical challenges.In order to overcome this concern,it is intended in this paper to modify acrylic fibers by different methods namely cationisation using the Crosscolor DRT then amidoximation using hydroxylamine hydrochloride and ammonium acetate.The resulted samples were dyed then with the bio-colorant extracted from grape marc.The effect of the pretreatment on fibers fine structure using X-ray diffraction and the scanning electron microscope(SEM)images and its correlation with the colour strength of the dyed fabrics was investigated.The dyeing parameters,such as dye bath pH and temperature on the performances of this dyeing process were studied.Good dyeing qualities and new shades varying from brown to grey and dark green have been obtained following process optimization,mordanting and modification of acrylic fibers by the technique of cationisation.

Patterns of genetic and morphometric diversity in the marbled crab(Pachygrapsus marmoratus, Brachyura, Grapsidae)populations across the Tunisian coast

The present study reports on population structure analysis of the marbled crab Pachygrapsus marmoratus （Fabricius, 1787） from the Tunisian coast, an appropriate location to study biogeographical processes because of the presence of a well-known discontinuous biogeographic area （the Siculo-Tunisian Strait）. Patterns of morphological and genetic variation of this highly dispersive and continuously distributed decapod species were assessed among its geographically close populations which cover almost the entire Tunisian coastline. A total of 386 specimens from nine sites were collected and examined for morphometric variability at 14 morphometric traits. The results of multivariate analyses of linear morphometric traits showed the existence of sexual dimorphism in this species by PERMANOVA （Permutational multivariate analysis of variance）. In addition, both CDA （Canonical discriminant analysis） and NPMANOVA （Non parametric MANOVA test） analyses revealed statistically significant differences among the studied locations for both sexes. Overall, the outcome of CDA analysis showed that over 87% of individuals could be assigned correctly to three regional groups in both sexes （North, Center and South）. Specifically, SIMPER （Similarity Percentages） analysis showed that carapace length, carapace width and merus length were major contributors to the morphometric separation between populations. The pattern of phenotypic variation suggested by morphometric analyses was found to be highly discordant with that suggested by the analysis of a mitochondrial marker （cytochrome oxidase I, COI）. Indeed, the results inferred from restriction fragment analysis of the COI in 180 crabs, suggested high genetic homogeneity. Very low levels of haplotype diversity （h） were found in almost all the studied populations, associated with non significant genetic distances for nearly all population comparisons. Explanations to these morphometric and mtDNA patterns as well as the discrepancy between them are discussed.

Phytochemical analysis and biological activities of Hertia cheirifolia L. roots extracts

Objective: To test the antioxidant, antimicrobial and a-glucosidase inhibitory activities of the roots extracts from Hertia cheirifolia(H. cheirifolia) L.Methods: Total phenolics and total flavonoids content of the different extracts were determined by colorimetric methods and reverse phase high-performance liquid chromatography(RP-HPLC) was performed to identify various chemical components.The different extracts were evaluated for antioxidant activities by 2,2-diphenyl-1-picrylhydrazyl(DPPH), 2,2-azino-bis-3-ethylenebenzothiozoline-6-sulfonic acid(ABTS·^+) and β-carotene bleaching tests and α-glucosidase inhibitory properties. The antimicrobial activity was carried out in vitro by the broth dilution method.Results: Trans-cinnamic acid, rutin hydrate, naringin and quercetin were the main compounds of the ethyl acetate extract from H. cheirifolia L. This extract has significant scavenging activity to decrease free radicals especially for DPPH and ABTS radicals. As well as, the ethyl acetate extract exhibited an antimicrobial property against bacterial strains. Bacillus licheniformis and Salmonella enterica were the most sensitive strains with minimum inhibitory concentration values of 0.156 mg/mL.Conclusion: The ethyl acetate extract was found to be selectively antioxidant and antimicrobial.