Phenotypic and Molecular Characterization of Staphylococcaceae and Enterobacteriaceae Species Isolated from Smoked, Dried, and Braised Fish Marketed in Ouagadougou

Background: To preserve its nutritional properties, fish must have good sanitary quality. The objective of this study was to investigate some pathogens contaminating smoked, dried, and braised fish marketed in Ouagadougou. Methodology: Potential pathogens of Enterobacteriaceae and Staphylococcus were screened in eight (8) species of processed fish. The investigation of the germs was carried out following the normative methods of microbiology. The identities of the strains were determined by API 20 E (BioMerieux S.A., France) and API STAPH (BioMerieux S.A., France) kits for Enterobacteriaceae and Staphylococcus species respectively. The uidA gene profile in Escherichia coli isolates was determined by simplex PCR. The identity of Staphylococcus aureus was confirmed by amplification of specific 23S rDNA regions and nuc gene profile with PCR. Results: A total of 235 fish samples were analyzed. A diversity of Enterobacteriaceae and Staphylococcus was detected. Twenty species of Enterobacteriaceae were identified among which, the most frequent were Escherichia coli, Salmonella sp, Raoultella ornithinolytica and Serratia odorifera, respectively in 22.6%, 4.3%, 28.9%, 17.4% of the samples analyzed. However, eleven species of Staphylococcus were identified among which, Staphylococcus xylosus, Staphylococcus aureus, Staphylococcus lugdunensis, and Staphylococcus sciuri were the most frequent with respective percentages of 47.7%, 23.4%, 12.8% and 10.6% of samples. For all the samples, the species frequently isolated were: Raoultella ornithinolytica, Escherichia coli, Serratia odorifera, Staphylococcus aureus, Staphylococcus xylosus, and Staphylococcus lugdunensis. The uidA gene specific to Escherichia coli was detected in 82.85% of strains (29/35). Amplification of the specific 23S rDNA region using staur primers was observed in 98% (49/50) of the isolated Staphylococcus aureus strains and the nuc gene was detected in 86% of Staphylococcus aureus strains. Conclusion: The isolated bacteria are potential pathogens involved in foodborne illnesses and intoxications. Effective sanitary safety systems must be implemented to guarantee the sanitary quality of fish supplied to consumers.

Experimental Study and Modeling of a Convective Dryer for Fruits and Vegetables

The present work deals with the study of the thermal performances of a convective dryer for fruits and vegetables. This dryer, operating with energy generated from the combustion of biomass in a boiler connected to a water/air heat exchanger could be a solution to the problematic of energy related to drying. An experimental and theoretical study is carried out on the temperature profile inside the dryer. For this purpose, 10.3 kg of tomatoes were dried on the experimental setup. The operation lasted about 16 hours and reduced the moisture content from 93.8% to 12% in wet basis. The overall thermal efficiency of the convective dryer during the trial is 10.76%. For the theoretical study, the dryer components (boiler, water/air exchanger and drying chamber) are first modeled individually;the different sub-programs are then coupled to form the convective dryer program. The method of global heat balances combined with the one called “ε-NUT” is used. The set of equations is discretized using the implicit method of finite differences, then solved with the Gauss algorithm in Fortran 90. The theoretical results obtained are in good agreement with those measured.

Numerical Models and Methods of Atmospheric Parameters Originating in the Formation of the Earth’s Climatic Cycle

Atmospheric models are physical equations based on the ideal gas law. Applied to the atmosphere, this law yields equations for water, vapor (gas), ice, air, humidity, dryness, fire, and heat, thus defining the model of key atmospheric parameters. The distribution of these parameters across the entire planet Earth is the origin of the formation of the climatic cycle, which is a normal climatic variation. To do this, the Earth is divided into eight (8) parts according to the number of key parameters to be defined in a physical representation of the model. Following this distribution, numerical models calculate the constants for the formation of water, vapor, ice, dryness, thermal energy (fire), heat, air, and humidity. These models vary in complexity depending on the indirect trigonometric direction and simplicity in the sum of neighboring models. Note that the constants obtained from the equations yield 275.156˚K (2.006˚C) for water, 273.1596˚K (0.00963˚C) for vapor, 273.1633˚K (0.0133˚C) for ice, 0.00365 in/s for atmospheric dryness, 1.996 in2/s for humidity, 2.993 in2/s for air, 1 J for thermal energy of fire, and 0.9963 J for heat. In summary, this study aims to define the main parameters and natural phenomena contributing to the modification of planetary climate. .

Modeling the Ionosphere during Quiet Time Variation at Ouagadougou in West Africa

Ionosphere parameters determination is used to characterize its composition in particles. These results have been compared to data curried from Ouagadougou station. The present study deals with Total Electron Contents (TEC) results determined by Thermosphere-Ionosphere-Electrodynamics General Circulation Model (TIEGCM) version 1.94 and International Reference Ionosphere (IRI) version 2012 during solar cycle 22. The minimum and maximum phases of solar cycle 22 are considered in this study for TEC determination. The station is located at Ouagadougou, in western Africa, characterized by its latitude (12.4°N) and longitude (358.5°E). The present study completes the two previous articles on hmF2 and foF2 parameters determination on the same station by comparison between TEC results carried out from TIEGCM and IRI models. So that, quiet time condition is determined by Aa (≤20 nT) for the five quietest days in each characteristic month of seasons. Rz values characterize minimum and maximum solar cycle phases.

Effects of Cereal Malts Used as Improver on Physico-Chemical, Nutritional and Sensory Characteristics of Wheat and Millet Composites Breads

The aim of this study was to optimize composite breads of wheat and whole millet flour by the use of natural improvers. Three types of local malted cereals were used as natural improvers. The millet flour was fermented with EPSs producing LAB strain prior to use. The technological characteristics of the composite flours were determined using an alveograph. The physico-chemical and nutritional characteristics of the composite breads were determined using standard methods and their sensory profiles were evaluated by a panel of 35 consumers. The alveograph results showed an increase in dough resistance, deformation and a decrease in extensibility and elasticity with the level of incorporation of millet flour. From the results of physico-chemical analyses of composite breads, no significant difference (p > 0.05) was observed in the use of the three types of local cereal malts except for the incorporation of 50% of the millet flour. The control sample presented the lowest acidity and dry matter value, the highest water content and pH value. No significant difference (p > 0.05) was observed in the use of the three types of cereal malts for the macronutrient contents of the composite bread samples. However, differences were observed according to the levels of incorporation. Macronutrients results showed an increase in protein content (11.17% ± 0.28% - 14.01% ± 0.10%/DM);crude fat content (1.86% ± 0.05% - 2.48% ± 0.20%/DM) and a decrease in carbohydrates content (85.36% ± 0.54% - 81.06% ± 0.36%/ DM). Regarding the content of mineral elements, significant differences (p < 0.05) were observed in the use of the three types of cereal malts for the incorporation of 15% (Mg and Fe content), 30% (Fe, Zn and K) and 50% (content of Zn, K, Na and Mg) of millet flour. The free amino acid profile revealed three essential amino acids such as valine, isoleucine and lysine. Breads incorporated with 30% of whole millet flour were the most appreciated by consumers.

Experimental Study of a Metallic Pressure Cooker Insulated with Kapok Wool

This work is devoted to an experimental study of metallic pressure cooker insulated with kapok wool, a vegetal biodegradable fiber. Experiments conducted on the cooling of hot water in the equipment revealed very low heat losses and a time constant of 60 hours on average. As a result, the equipment makes it possible to finish cooking meals only thanks to the heat stored at the beginning of cooking and keeps cooked dishes warm for long hours. The thermal phase shift of the pressure cooker is around 7?h. Cooking tests conducted on some local dishes revealed about 70% butane gas savings for cooking cowpea and white rice, 38% for cooking fatty rice, 75% for pasta and couscous, and 30% for cooking potato stew. These results show that this technology can contribute to minimizing?energy consumption in the restaurant sector.

Physicochemical and Biochemical Composition of Balanites aegyptiaca Seed and Seed Oil from Burkina Faso

In Burkina Faso, the seeds of Balanites aegyptiaca have been considered to be potentially high lipid, protein and mineral sources but seem to be neglected and sometimes unknown by communities. This study aims to complete data on seed oil and detail on amino acids and minerals composition of the seed of B. aegyptiaca for better use. Physicochemical parameters such as moisture and ash content, saponification index, iodine, acid value, peroxide value and the melting point were determined. Seed oil triglycerides, fatty acids and amino acids have been estimated throughout this study. The moisture and ash content were 3.70% ± 0.1% and 2.90% ± 0.2%, respectively. The saponification and iodine values were respectively 181.96 ± 0.4 mg KOH/g of oil and 104.86 ± 0.6 g of iodine/100 g of oil. Polyunsaturated fatty acid content (50.94%) was the most important. Triglyceride (LLO, 22.4%) was the major triglyceride. 9 essential amino acids and 9 non-essential amino acids were identified. Phenylalanine (11697.82 ± 0.00 mg/kg) was the most important essential amino acid. The content of 21 minerals was determined and the most important was potassium (9323.13 ± 0.01 mg/kg). Ca/P, Ca/Mg, Ca/Mg and Na/K ratios were 0.34;1.18;0.04 and 0.19 respectively. B. aegyptiaca seed oil is a source of multiple nutritional values and can be used by the population for multipurpose.

Peak of Electron Density in F2-Layer Parameters Variability at Quiet Days on Solar Minimum

This study deals with Peak of electron density in F2-layer sensibility scale during quiet time on solar minimum. Peaks of electron density in F2-layer (NmF2) values at the quietest days are compared to those carried out from the two nearest days (previous and following of quietest day). The study uses International Reference Ionosphere (IRI) for ionosphere modeling. The located station is Ouagadougou, in West Africa. Solar minimum of phase 22 is considered in this study. Using three core principles of ionosphere modeling under IRI running conditions, the study enables to carry out Peak of electron density in F2-layer values during the quietest days of the characteristic months for the four different seasons. These parameters are compared to those of the previous and the following of the quietest days (the day before and following each quietest selected day) at the same hour. The knowledge of NmF2 values at the quietest days and at the two nearest days enables to calculate the relative error that can be made on this parameter. This calculation highlights insignificant relative errors. This means that NmF2 values at the two nearest days of each quietest day on solar minimum can be used for simulating the quietest days’ behavior. NmF2 values obtained by running IRI model have good correlation with those carried out by Thermosphere-Ionosphere-Electrodynamics-General Circulation Model (TIEGCM).

Soil Macrofauna Behaviour in the Presence of Pesticides and Organic Amendments

Insects attack vegetable crop, leading to an overuse of pesticides. Organic amendments are recommended to increase soil fertility. The impact of two insecticides, on soil macrofauna under rainfed cultivation of tomato (Lycopersicon esculentum L.) with or without organic amendments was evaluated. The insecticides were Lambda Super (lambda-cyhalothrin) and Dursban (chlorpyrifos ethyl) and organic amendments were compost, pig manure and cattle manure. An experimental device in split-plot with three replicate was installed. Pesticides were applied periodically each week during the experiment. The results have showed that with organic amendments, Lambda super increased the earthworm population compared to the control. Indeed, we found 42 individuals/m2 with compost;1 individuals/m2 with pig manure and 27 individuals/m2 with cattle manure but the control was 0 individuals/m2 in the presence of Lambda super without organic amendment. Similarly, Dursban increased the population of earthworms in the presence of organic amendment with 11, 37 and 91 individuals/m2 respectively for compost, pig manure and cattle manure. Lambda super with compost has led to a total elimination of termites. In the presence of Dursban, a decrease in the number of termites was observed when adding cattle manure (11 individuals/ m2) compared to the control containing only Dursban (21 individuals/m2). Better distribution of macrofauna was obtained with treatment with Dursban and compost (E = 0.99 for earthworms and E = 0.96 for termites). All organic amendments used in our study stimulated earthworm population, but more in the presence of Lambda super or Dursban.

Microbiological Quality of Fresh and Grilled Mutton Sold in Ouagadougou, Burkina Faso

In Ouagadougou, grilled meats in the form of pieces and brochettes are very popular and well-known to consumers. The aim of this study was to assess the microbial quality of mutton meat sold in Ouagadougou, Burkina Faso. A total of 120 samples were collected from 20 meat grillers 60 samples of fresh meat and 60 samples of grilled meat. The sampling was done between the month of August 2018 and the month of February 2019. The samples were analyzed according to standard methods. The test performed were counts of Aerobic Mesophilic Bacteria (AMB), yeasts and molds, enterobacteria, Campylobacter spp, Staphylococcus aureus, Bacillus cereus, Brucella spp, Pseudomonas aeruginosa, the search for salmonella, and the detection of antibiotics residues. Results showed a high count of AMB (8.77 and 6.78 log UFC/g);enterobacteria (6.58 and 3.05 log UFC/g), Staphylococcus aureus (6.45 and 4.35 log UFC/g), Bacillus cereus (6.98 and 4.52 log UFC/g), Campylobacter (6.03 and 3.86 log UFC/g), yeasts and molds (4.80 and 3.26 log UFC/g) and Pseudomonas aeruginosa (0.45 and 0.15 log UFC/g), respectively in fresh meat and grilled meat. Presumptive Salmonella was found in 95% of fresh meat samples and in 75% of grilled meat samples. In the tested samples, no Brucella spp were detected. However, residues of antibiotics were found in 5% of fresh meat samples and 5% of grilled meat samples. Means of moisture and pH were respectively 74.91% and 6.05% for fresh meat and 53.21% and 6.06% for grilled meat. The average microbial counts recorded in fresh and grilled meat are significantly high and indicate poor hygiene in the raw material and ready-to-eat meat. Good practices of hygiene and processing guides should be developed for the meat grilling value chain actors to reduce contamination risks.

Performance Assessment of a Box Type Solar Cooker Using Jatropha Oil as a Heat Storage Material

Solar cookers are a good option in developing countries with high solar potential for environmentally friendly cooking and reduced pressure on forests. However, they are still affected by the intermittency of the sun. In order to overcome this problem, in this work, a box type solar cooker integrated Jatropha oil as a heat storage material is fabricated and experimented with. The design was examined with a maximum stagnation temperature of 157.7&deg;C. The recorded cooking power vanished between 78.4 and 103.6 W, while thermal efficiency varied from 41.26% to 58.78%. The energy transfer cycle test, including charge and discharge revealed that 91.18% of the heat lost through the cooker could be recovered by the heat storage unit and a large amount is restored to the system during cloudiness or a temperature perturbation.

Modeling the Impact of Desert Aerosols on the Solar Radiation of a Mini Solar Central Photovoltaic (PV)

This work focuses on modeling the impact of desert aerosols on a mini central solar photovoltaic (PV). Our studied physical model is comparable to a multilayer. We have described and discretized the mathematical equations which govern the physical model. Also, we analyzed the influence of the parameters τa and X on the solar radiation received at the surface of solar PV modules. The results of the study taken from Figures 6(a)-(d) representing the variations of the global solar radiation on the solstices and equinoxes as well as the 21 of the months of the year days understood show that: if τa = 0 and X = 0, IC = 67.87%;if τa = 0.5 and X = 0.5, IC = 21%;if τa = 0.8 and X = 0.8, IC = 12% and if τa = 1.5 and X = 1.5 then IC = 4%. These results show that desert aerosols significantly influence the global solar radiation received. Unfortunately, this influence lowers the productivity of the central solar PV in general.

Comparative Study of foF2 during Quiet Geomagnetic Activity with URSI and CCIR Predictions during the Phase Minimum of Solar Cycle 22

This paper investigates the performance of the latest International Reference Ionosphere model to predict the critical frequency at low latitudes in the African region. The variability of the critical frequency of the F2 layer of the ionosphere (foF2) is studied for the different seasons of the phase minimum of solar cycle 22 during quiet geomagnetic activity at the Ouagadougou station. The data used are those provided by the ionosonde and the predictions of the two subprograms: International Radio Consultative Committee (CCIR) and International Radio-Scientific Union (URSI) of the 2016 version of the International Reference Ionosphere model. This study shows that, in general, URSI and CCIR of the IRI-2016 model are able to reproduce fairly well the variability of the critical frequency of the F2 layer of the ionosphere at low latitudes during the phase minimum at the Ouagadougou station. However, the model shows an almost homogeneous overestimation of the foF2 during the four seasons studied. The good response is observed between 0700 TL and 1900 TL for the available data. The agreement between the subroutine responses and the observed results is between reasonable and poor. The best match state response is obtained in winter with the CCIR subroutine. These results show that there is a need to improve both CCIR and URSI subroutines of the IRI-2016 model in low latitudes in the African region.

Modeling Submicron Particles Collection in Laminar Forced Convection Gas Flow by a Rectangular Venturi Scrubber

Venturi scrubbers are usually used for large particles cleaning in turbulent gaseous flow. In this work, submicron particles scrubbing in laminar forced convection dusty air flow in a rectangular venturi scrubber have been numerically simulated. Hydrodynamics effects and scrubbing process are investigated in detail. Results are presented as flow velocity, axial pressure, streamlines pattern, particles and droplets mass fraction profile, and collect efficiency. They show that venturi scrubbers can be efficient for submicron particles scrubbing. In fact, a better collect efficiency is obtained at high particles-droplets residence time, high ratio droplets concentration/particles concentration, low venturi diameter ratioand low Reynolds numbers. There is a critical Reynolds number value for which the collect efficiency becomes very low and tends to be constant.

Evaporation and Combustion of a Drop of Liquid Fuel—A Review

The accelerated depletion of oil reserves and the often exorbitant cost of fossil fuels contribute to the development of fuels from renewable sources. The objective of this work is to analyze the influence of the properties of renewable fuels on their evaporation in natural convection, their combustion and their use in internal combustion engines. A summary of the various numerical and experimental works from the literature has been presented in this work. This work focuses on the numerical modelling of the natural convection evaporation of an isolated drop of a liquid fuel in natural convection. The transfers in the liquid and vapour phases are described by the conservation equations of mass and species, momentum and energy. The main feature of this work is the consideration of advection, azimuthal angle and thickness of the vapour phase of the drop during evaporation of the drop.

Electron Bulk Surface Density Effect on Critical Frequency in the F2-Layer

Ionosphere layer is the atmosphere region which reflects radio waves for telecommunication. The density in particles in this layer influences the quality of communication. This study deals with the effects of Total Electron Contents (TEC) on the critical frequency of radio waves in the F2-layer. Total Electron Contents parameter symbolizes electron bulk surface density in ionosphere layer. Above critical frequency value in F2 layer (foF2), radio waves pass through ionosphere. The knowledge of this value enables to calibrate transmission frequencies. In this study, we consider TEC effects on foF2 under quiet time conditions during the maximum and the minimum of solar cycle 22, at Ouagadougou station, in West Africa. The study also considers the effects of seasons and the hourly variability of TEC and foF2. This work shows winter anomaly on foF2 and TEC on minimum and maximum of solar cycle phase respectively. Running International Reference Ionosphere (IRI) model enables to carry out the effects of TEC on foF2 by use of their monthly average values. This leads to a new approach to calibrate radio transmitters.

Fermentation Effect on the Nutrient and Antinutrient Composition of <i>Senegalia macrostachya</i>and <i>Parkia biglobosa</i>Seeds: A Comparative Study

This study aimed to evaluate the effect of the fermentation on the nutrient and antinutrient composition of Senegalia macrostachya and Parkia biglobosa seeds. For condiments production, the raw seeds were cleaned, cooked, drained, washed, cooked, drained and fermented. Results show that the pH increased (from 6.09 to 7.76 and from 6.99 to 7.92) from the onset of the fermentation till 48 h during fermentation of Senegalia macrostachya and Parkia biglobosa seeds respectively. Biochemical analyses revealed a slight increase in water content, ashes, lipids, minerals and a decrease in total carbohydrate for the two products. The fermentation of Senegalia macrostachya and Parkia biglobosa seeds reduced the initial phytates content by 38.21% and 41.37% respectively. Fermented seeds of Senegalia macrostachya are rich in protein (59.59 ± 0.74 g/100g DM), potassium and magnesium (1076.74 ± 37.49 mg/100g DM and 111.63 ± 06 mg/100g DM respectively) with an appreciable iron content (26.27 ± 0.06 mg/100g DM). Given its nutritional composition, fermented seeds of Senegalia macrostachya could be used as an alternative to soumbala in areas where it is not available.

Impact of Health and Nutrition Interventions in the Prevention and Recovery of Child Malnutrition in Sub-Saharan Africa from 2010 to 2020: Review Article

Malnutrition is still a widespread public health problem worldwide. The objective of this review is to identify the most effective nutrition programs used in the prevention and management of child malnutrition in children under five years. Also, it is to suggest courses of action to improve interventions in the fight against malnutrition. The promotion of exclusive breastfeeding and food diversification improves the mothers’ knowledge and contributes to prevention of malnutrition. Food supplementation increases the weight/height ratio of malnourished children. The two programs combined bring better results on mothers’ feeding practices and children’s anthropometric data. This review has highlighted the importance of food supplementation and nutritional education in the management and prevention of child malnutrition. In addition, the combination of several approaches to good dietary practices brings better results. Finally, primary prevention can protect children from the harmful consequences of malnutrition.

Identification of Bacteria Strains Isolated in Urinary Tract Infections and Their Antibiotic Susceptibility at the National Public Health Laboratory, Ouagadougou

Background: Urinary tract infections are still a real public health concern. The aim of this study was to identify the bacteria strains involved in urinary tract infections and to determine their antibiotic resistance profiles. Methods: Two kinds of studies were performed. The retrospective study was carried out for 2 years (from January 2018 to December 2019), and the prospective study was extended over a period of 6 months (from January to June 2020). Isolation and identification of bacteria strains were performed using conventional microbiology techniques. The strains’ resistance profiles were determined by the diffusion method on Mueller-Hinton according to the criteria of EUCAST- 2015. Fourteen (14) antibiotic discs were used depending on the isolated germ. Results: A total of 187 bacterial strains were isolated from 82 men and 105 women. Among the germs identified, 77.54% were Enterobacteriaceae and 13.36% were cocci strains. Non-fermentative gram-negative bacilli accounted for 9.08% of the isolated bacteria. The results showed that the majority of Enterobacteriaceae strains were resistant to beta-lactams: 100% to amoxicillin, 98.75% to amoxicillin + clavulanic acid, 41.76% to ceftriaxone, and 43.14% to ceftazidime. These findings were obtained with fluoroquinolones and aminoglycosides: 50.09% with ciprofloxacin, 54.04% with norfloxacin, and 22.58% with amikacin. 8.75% of the Enterobacteriaceae strains tested were resistant to imipenem. The same trends were observed with non-fer- mentative bacteria. As for the gram-positive bacteria isolated during our study, 13.33% were resistant to vancomycin, 21.05% to gentamicin, 94.12% to penicillin G, 88.89% to ampicillin, 77.78% to cefotaxime, 63.63% to kanamycin, and 52.63% to erythromycin. Conclusion: This study revealed, as in other studies, that Enterobacteriaceae strains remain the most incriminated bacteria strains in urinary tract infections, with a strong resistance to antibiotics. It is important that actions be taken to reduce the incidence of urinary tract infections and mitigate the spread of resistant bacteria.

Contribution of the International Reference Ionosphere-2016 Model in the Evidence of the Winter Anomaly

This study is a contribution to the estimation of the winter anomaly in the F2 layer of the ionosphere at low latitudes. The aim is to study the variability of the virtual height (hmF2) of the F2 region of the ionosphere through the predictions of the latest International Reference Ionosphere model (IRI-2016). The present work allows analyzing the temporal evolution of hmF2 according to the different phases of three (3) solar cycles during the quiet geomagnetic activity to estimate the seasonal anomaly at the Ouagadougou station. The analysis of the seasonal profiles shows that the variability of hmF2 is: 1) strongly linked to the solar cycle activity, 2) dependent on the season and 3) variable from one cycle to the next for the same phase. It appears that hmF2 increases during the ascending phase to reach its maximum value at the phase maximum. During the descending phase, it decreases until the phase minimum where it finds its minimum value. The difference between winter and summer on the hmF2 values for each phase of the cycle is obtained at the phase minimum and is estimated to be at least 16 km. In low latitudes, solar irradiation is greater in summer than in winter. From this study, hmF2 values are larger in winter compared to summer indicating an anomaly in the virtual height of the F2 layer of the ionosphere through the predictions of IRI-2016 at the Ouagadougou station.