Various mouse models to study dengue have been described by different authors, some of them using immunodeficient or some using humanized mice. Our group reported previously a deadly murine model, which used the intra...Various mouse models to study dengue have been described by different authors, some of them using immunodeficient or some using humanized mice. Our group reported previously a deadly murine model, which used the intracranial inoculum of highly virulent Dengue virus (DENV) in immune competent mouse. Here we present a model of immune competent mouse (C57BL/6), infected subcutaneously by the same highly virulent DENV (DENV3 genotype I). In this immunocompetent systemic mice model, the cytokine levels and hematological parameters such as total and differential leukocyte and platelets counts, together with weight loss, were considered important monitoring parameters, allowing a better understanding of the systemic human disease. Mice were inoculated subcutaneously and evaluated by the percentage weight variation as well as the clinical signs. Hematological parameters and cytokines levels were measured and viral titration in brain tissue or serum neutralization was performed to confirm mice infection. The subcutaneously DENV inoculated mice showed weight loss after infection, but they did not show any other clinical signs. The leukocytes and platelets decreased after subcutaneous inoculation. The cytokines TNF alpha and IFN gamma increased after infection in mice. The subcutaneous model provided scope for improved understanding of the dengue pathogenesis, as well as possible mechanism for protection to subsequent mouse infected by intracranial route in mice. This model could be used to study the vertebrate immune response and evaluation of drugs or vaccine against dengue virus.展开更多
The study PV/diesel system is a stand-alone microgrid powered by the PV/diesel combination without production storage. The study focused on optimising PV/diesel production by monitoring data. It also referred to a com...The study PV/diesel system is a stand-alone microgrid powered by the PV/diesel combination without production storage. The study focused on optimising PV/diesel production by monitoring data. It also referred to a comparison of sensitive factors in PV/diesel production. This study implemented structural and non-structural factors of the said system. A literature search was conducted to determine the factors involved. So, factors such as system autonomy, energy quality, system stability and data monitoring were considered for the study. Thus, after a detailed presentation of the data monitoring, a comparison based on the method, Analysis of Failure Modes, their Effects and Criticalities (FMEA) was carried out. At the end of the comparison, a hierarchy of parameters in the exploitation of the energy production of autonomous microgrids was made. From its results, it emerges a good consideration of the factor “data monitoring” in the management of the system studied. The results obtained confirm the importance of data monitoring for a better optimization of energy production. A monitoring program or procedure has been developed according to the originality that the present study has identified. The study also made it possible to evaluate the performance of data monitoring for the energy production of photovoltaic systems in general and hybrid PV/diesel systems in particular.展开更多
文摘Various mouse models to study dengue have been described by different authors, some of them using immunodeficient or some using humanized mice. Our group reported previously a deadly murine model, which used the intracranial inoculum of highly virulent Dengue virus (DENV) in immune competent mouse. Here we present a model of immune competent mouse (C57BL/6), infected subcutaneously by the same highly virulent DENV (DENV3 genotype I). In this immunocompetent systemic mice model, the cytokine levels and hematological parameters such as total and differential leukocyte and platelets counts, together with weight loss, were considered important monitoring parameters, allowing a better understanding of the systemic human disease. Mice were inoculated subcutaneously and evaluated by the percentage weight variation as well as the clinical signs. Hematological parameters and cytokines levels were measured and viral titration in brain tissue or serum neutralization was performed to confirm mice infection. The subcutaneously DENV inoculated mice showed weight loss after infection, but they did not show any other clinical signs. The leukocytes and platelets decreased after subcutaneous inoculation. The cytokines TNF alpha and IFN gamma increased after infection in mice. The subcutaneous model provided scope for improved understanding of the dengue pathogenesis, as well as possible mechanism for protection to subsequent mouse infected by intracranial route in mice. This model could be used to study the vertebrate immune response and evaluation of drugs or vaccine against dengue virus.
文摘The study PV/diesel system is a stand-alone microgrid powered by the PV/diesel combination without production storage. The study focused on optimising PV/diesel production by monitoring data. It also referred to a comparison of sensitive factors in PV/diesel production. This study implemented structural and non-structural factors of the said system. A literature search was conducted to determine the factors involved. So, factors such as system autonomy, energy quality, system stability and data monitoring were considered for the study. Thus, after a detailed presentation of the data monitoring, a comparison based on the method, Analysis of Failure Modes, their Effects and Criticalities (FMEA) was carried out. At the end of the comparison, a hierarchy of parameters in the exploitation of the energy production of autonomous microgrids was made. From its results, it emerges a good consideration of the factor “data monitoring” in the management of the system studied. The results obtained confirm the importance of data monitoring for a better optimization of energy production. A monitoring program or procedure has been developed according to the originality that the present study has identified. The study also made it possible to evaluate the performance of data monitoring for the energy production of photovoltaic systems in general and hybrid PV/diesel systems in particular.
