BACKGROUND Diabetic nephropathy(DN),affecting half of diabetic patients and contributing significantly to end-stage kidney disease,poses a substantial medical challenge requiring dialysis or transplantation.The nuance...BACKGROUND Diabetic nephropathy(DN),affecting half of diabetic patients and contributing significantly to end-stage kidney disease,poses a substantial medical challenge requiring dialysis or transplantation.The nuanced onset and clinical progression of kidney disease in diabetes involve consistent renal function decline and persistent albuminuria.AIM To investigate Tiliroside's(Til)protective effect against diabetic nephropathy(DN)in rats under diabetic conditions.METHODS Five groups of six rats each were included in this study:Rats treated with DMSO by intraperitoneal injection as controls,those treated with STZ by intraperitoneal injection,those treated with STZ+Til(25 mg/kg body weight[bwt])or Til(50 mg/kg bwt),and those treated with anti-diabetic medication glibenclamide(600μg/kg bwt).Biochemical markers,fasting blood glucose,food intake,kidney weight,antioxidant enzymes,inflammatory and fibrotic markers,and renal injury were monitored in different groups.Molecular docking analysis was performed to identify the interactions between Til and its targeted biomarkers.RESULTS Til significantly reduced biochemical markers,fasting blood glucose,food intake,and kidney weight and elevated antioxidant enzymes in diabetic rats.It also mitigated inflammatory and fibrotic markers,lessened renal injury,and displayed inhibitory potential against crucial markers associated with DN as demonstrated by molecular docking analysis.CONCLUSION These findings suggest Til's potential as a therapeutic agent for DN treatment,highlighting its promise for future drug development.展开更多
Chemically extracted acellular nerve allografts loaded with brain-derived neurotrophic fac- tor-transfected or ciliary neurotrophic factor-transfected bone marrow mesenchymal stem cells have been shown to repair sciat...Chemically extracted acellular nerve allografts loaded with brain-derived neurotrophic fac- tor-transfected or ciliary neurotrophic factor-transfected bone marrow mesenchymal stem cells have been shown to repair sciatic nerve injury better than chemically extracted acellular nerve allografts alone, or chemically extracted acellular nerve allografts loaded with bone marrow mesenchymal stem cells. We hypothesized that these allografts compounded with both brain-derived neurotrophic factor- and ciliary neurotrophic factor-transfected bone marrow mesenchymal stem cells may demonstrate even better effects in the repair of peripheral nerve injury. We cultured bone marrow mesenchymal stem cells expressing brain-derived neuro- trophic factor and/or ciliary neurotrophic factor and used them to treat sciatic nerve injury in rats. We observed an increase in sciatic functional index, triceps wet weight recovery rate, myelin thickness, number of myelinated nerve fibers, amplitude of motor-evoked potentials and nerve conduction velocity, and a shortened latency of motor-evoked potentials when al- lografts loaded with both neurotrophic factors were used, compared with allografts loaded with just one factor. Thus, the combination of both brain-derived neurotrophic factor and cili- ary neurotrophic factor-transfected bone marrow mesenchymal stem cells can greatly improve nerve injury.展开更多
目的探讨鼻中隔后端犁骨切除后鼻孔成形术治疗先天性后鼻孔闭锁的疗效。方法回顾性分析深圳市儿童医院2017年3月至2019年6月收治的5例先天性后鼻孔闭锁患儿的资料,其中男2例,女3例;年龄33 d至2岁4个月;2例双侧先天性后鼻孔闭锁(CHARGE...目的探讨鼻中隔后端犁骨切除后鼻孔成形术治疗先天性后鼻孔闭锁的疗效。方法回顾性分析深圳市儿童医院2017年3月至2019年6月收治的5例先天性后鼻孔闭锁患儿的资料,其中男2例,女3例;年龄33 d至2岁4个月;2例双侧先天性后鼻孔闭锁(CHARGE综合征),1例单侧后鼻孔闭锁和单侧后鼻孔狭窄(Crouzon综合征);2例单侧先天性后鼻孔闭锁。所有病例在3.0 mm 0度耳内镜经鼻腔切除鼻中隔后端骨质、去除犁骨、闭锁板骨质并打磨患侧翼内板,连通双侧后鼻孔。术后不放置长期扩张支架。术后2周、1、3、6、12个月常规随访,最长随访超过2年。观察手术疗效。结果5例患儿均恢复良好的鼻腔通气功能,随访期间未见后鼻孔造孔出现再次狭窄和闭锁。无其他异常并发症出现。结论耳内镜下鼻中隔后端、犁骨、闭锁板切除术治疗后鼻孔闭锁可行,且术后可以不放置长期扩张支架,手术疗效确定,造孔无再次狭窄或闭锁。展开更多
Objective: Acute lung injury(ALI) is a serious respiratory dysfunction caused by pathogen or physical invasion. The strong induced inflammation often causes death. Tanshinone ⅡA(Tan-ⅡA) is the major constituent of S...Objective: Acute lung injury(ALI) is a serious respiratory dysfunction caused by pathogen or physical invasion. The strong induced inflammation often causes death. Tanshinone ⅡA(Tan-ⅡA) is the major constituent of Salvia miltiorrhiza Bunge and has been shown to display anti-inflammatory effects. The aim of the current study was to investigate the effects of Tan-ⅡA on ALI.Methods: A murine model of lipopolysaccharide(LPS)-induced ALI was used. The lungs and serum samples of mice were extracted at 3 days after treatment. ALI-induced inflammatory damages were confirmed from cytokine detections and histomorphology observations. Effects of Tan-ⅡA were investigated using in vivo and in vitro ALI models. Tan-ⅡA mechanisms were investigated by performing Western blot and flow cytometry experiments. A wound-healing assay was performed to confirm the Tan-ⅡA function.Results: The cytokine storm induced by LPS treatment was detected at 3 days after LPS treatment, and alveolar epithelial damage and lymphocyte aggregation were observed. Tan-ⅡA treatment attenuated the LPS-induced inflammation and reduced the levels of inflammatory cytokines released not only by inhibiting neutrophils, but also by macrophage. Moreover, we found that macrophage activation and polarization after LPS treatment were abrogated after applying the Tan-ⅡA treatment. An in vitro assay also confirmed that including the Tan-ⅡA supplement increased the relative amount of the M2 subtype and decreased that of M1. Rebalanced macrophages and Tan-ⅡA inhibited activations of the nuclear factor-κB and hypoxia-inducible factor pathways. Including Tan-ⅡA and macrophages also improved alveolar epithelial repair by regulating macrophage polarization.Conclusion: This study found that while an LPS-induced cytokine storm exacerbated ALI, including Tan-ⅡA could prevent ALI-induced inflammation and improve the alveolar epithelial repair, and do so by regulating macrophage polarization.展开更多
Sparse Representation based Classification （SRC） has emerged as a new paradigm for solving recognition problems. This paper presents a constraint sampling feature extraction method that improves the SRC recognition ...Sparse Representation based Classification （SRC） has emerged as a new paradigm for solving recognition problems. This paper presents a constraint sampling feature extraction method that improves the SRC recognition rate. The method combines texture and shape features to significantly improve the recognition rate. Tests show that the combined constraint sampling and facial alignment achieves very high recognition accuracy on both the AR face database （99.52%） and the CAS-PEAL face database （99.54%）.展开更多
Bionic inspiration from human thumb and index finger was the drive to design a high-performance two-finger dexterous hand.The size of each phalanx and the motion range of each joint in the human thumb and index finger...Bionic inspiration from human thumb and index finger was the drive to design a high-performance two-finger dexterous hand.The size of each phalanx and the motion range of each joint in the human thumb and index finger were summarized,and the features of three grasping patterns were described in detail.Subsequently,a two-finger dexterous bionic hand with 6 Degrees of Freedom(DoFs)was developed.Both the mechanical thumb and index finger were made up of three rigid phalanx links and three mechanical rotation joints.Some grasp-release tests validated that the bionic hand can perform three grasping patterns:power grasp,precision pinch and lateral pinch.The grasping success rates were high under the following cases:(1)when power grasping was used to grasp a ring with external diameter 20 mm-140 mm,a cylinder with mass<500 g,or objects with cylinder,sphere or ellipsoid shape;(2)when the precision pinch was used to grasp thin or small objects;(3)when the lateral pinch was used to grasp low length-to-width ratio of objects.The work provided a method for developing two-fimger bionic hand with three grasping patterns,and further revealed the linkage between the difference in fimger structure and size and the hand manipulation dexterity.展开更多
Cerebral ischemia(CI)is the world’s second-largest lethal disease,with a high recurrence and teratogenic rate.Traditional Chinese medicine(TCM)YQHX(a pharmaceutical preparation from herbs)has a certain effect in the ...Cerebral ischemia(CI)is the world’s second-largest lethal disease,with a high recurrence and teratogenic rate.Traditional Chinese medicine(TCM)YQHX(a pharmaceutical preparation from herbs)has a certain effect in the clinical treatment of CI,while its underlying mechanism remains largely undetermined.To explore the potential mechanism,we used network pharmacology and molecular docking in the present study.TCMSP and CNKI databases were used to explore the active ingredients of YQHX;the Pharmmapper database was used to get the ingredient targets;the OMIM,GeneCards,and DisGeNET databases were used to obtain the disease targets;the Venn diagram was used to obtain the intersection targets,the Cytoscape was used to visualize results and plug-in MCODE to obtain core targets;the Metascape database was used to perform GO and KEGG pathway enrichment analyses on core targets.The top 20 KEGG pathway enrichment pathways were used to construct the“ingredient-target-pathway”network by Cytoscape;the top 10 ingredients and the top five protein targets were used for molecular docking with AutoDock Vina software,and PyMoL and Ligplus software were used to visualize the results.A total of 83 active ingredients were screened from YQHX.Moreover,432 corresponding targets,2005 disease-related targets,and 140 drug-disease intersection targets were obtained.GO biological function and KEGG pathway enrichment analyses yielded 507 biological function entries and 141 signaling pathways.KEGG pathway enrichment was mainly involved in cell proliferation,adhesion,migration,and other processes.Molecular docking results showed that the key ingredients and core targets screened had a strong binding activity,including EGFR,MAP2K1,and KDR.The combination of miltionone I and miltiodiol was relatively stable.The main biological mechanism of YQHX in the treatment of CI might play a role through the signaling pathway related to the tyrosine kinase receptor,which was also the improvement of the theory of“benefiting qi and activating blood circulation for promoting the production of blood and blood vessels”.展开更多
To the Editor：Asthma poses a serious health problem globally.People of all ages can be affected by this chronic airway disorder that,when uncontrolled,may place severe limits on daily life and is sometimes fatal.The ...To the Editor：Asthma poses a serious health problem globally.People of all ages can be affected by this chronic airway disorder that,when uncontrolled,may place severe limits on daily life and is sometimes fatal.The prevalence of asthma is increasing in most countries throughout the world,especially among children.[1]During the past decades,many scientific advances have been made to improve our understanding of asthma and our ability to manage and control it effectively.These advances are mainly reflected by related scientific articles,particularly by those most influential papers.展开更多
基金the Ethical Committee of Shanxi Bethune Hospital(Approval No.YXLL-2023-222).
文摘BACKGROUND Diabetic nephropathy(DN),affecting half of diabetic patients and contributing significantly to end-stage kidney disease,poses a substantial medical challenge requiring dialysis or transplantation.The nuanced onset and clinical progression of kidney disease in diabetes involve consistent renal function decline and persistent albuminuria.AIM To investigate Tiliroside's(Til)protective effect against diabetic nephropathy(DN)in rats under diabetic conditions.METHODS Five groups of six rats each were included in this study:Rats treated with DMSO by intraperitoneal injection as controls,those treated with STZ by intraperitoneal injection,those treated with STZ+Til(25 mg/kg body weight[bwt])or Til(50 mg/kg bwt),and those treated with anti-diabetic medication glibenclamide(600μg/kg bwt).Biochemical markers,fasting blood glucose,food intake,kidney weight,antioxidant enzymes,inflammatory and fibrotic markers,and renal injury were monitored in different groups.Molecular docking analysis was performed to identify the interactions between Til and its targeted biomarkers.RESULTS Til significantly reduced biochemical markers,fasting blood glucose,food intake,and kidney weight and elevated antioxidant enzymes in diabetic rats.It also mitigated inflammatory and fibrotic markers,lessened renal injury,and displayed inhibitory potential against crucial markers associated with DN as demonstrated by molecular docking analysis.CONCLUSION These findings suggest Til's potential as a therapeutic agent for DN treatment,highlighting its promise for future drug development.
文摘Chemically extracted acellular nerve allografts loaded with brain-derived neurotrophic fac- tor-transfected or ciliary neurotrophic factor-transfected bone marrow mesenchymal stem cells have been shown to repair sciatic nerve injury better than chemically extracted acellular nerve allografts alone, or chemically extracted acellular nerve allografts loaded with bone marrow mesenchymal stem cells. We hypothesized that these allografts compounded with both brain-derived neurotrophic factor- and ciliary neurotrophic factor-transfected bone marrow mesenchymal stem cells may demonstrate even better effects in the repair of peripheral nerve injury. We cultured bone marrow mesenchymal stem cells expressing brain-derived neuro- trophic factor and/or ciliary neurotrophic factor and used them to treat sciatic nerve injury in rats. We observed an increase in sciatic functional index, triceps wet weight recovery rate, myelin thickness, number of myelinated nerve fibers, amplitude of motor-evoked potentials and nerve conduction velocity, and a shortened latency of motor-evoked potentials when al- lografts loaded with both neurotrophic factors were used, compared with allografts loaded with just one factor. Thus, the combination of both brain-derived neurotrophic factor and cili- ary neurotrophic factor-transfected bone marrow mesenchymal stem cells can greatly improve nerve injury.
文摘目的探讨鼻中隔后端犁骨切除后鼻孔成形术治疗先天性后鼻孔闭锁的疗效。方法回顾性分析深圳市儿童医院2017年3月至2019年6月收治的5例先天性后鼻孔闭锁患儿的资料,其中男2例,女3例;年龄33 d至2岁4个月;2例双侧先天性后鼻孔闭锁(CHARGE综合征),1例单侧后鼻孔闭锁和单侧后鼻孔狭窄(Crouzon综合征);2例单侧先天性后鼻孔闭锁。所有病例在3.0 mm 0度耳内镜经鼻腔切除鼻中隔后端骨质、去除犁骨、闭锁板骨质并打磨患侧翼内板,连通双侧后鼻孔。术后不放置长期扩张支架。术后2周、1、3、6、12个月常规随访,最长随访超过2年。观察手术疗效。结果5例患儿均恢复良好的鼻腔通气功能,随访期间未见后鼻孔造孔出现再次狭窄和闭锁。无其他异常并发症出现。结论耳内镜下鼻中隔后端、犁骨、闭锁板切除术治疗后鼻孔闭锁可行,且术后可以不放置长期扩张支架,手术疗效确定,造孔无再次狭窄或闭锁。
基金supported by National Natural Science Foundation of China (No. 81570020 and 82170033)Natural Science Foundation of Shanghai (21ZR1479200)Shanghai Changhai Hospital Scientific Research Fund (2019SLZ002, 2019YXK018, CHJG2019029 and CHPY2021A05)。
文摘Objective: Acute lung injury(ALI) is a serious respiratory dysfunction caused by pathogen or physical invasion. The strong induced inflammation often causes death. Tanshinone ⅡA(Tan-ⅡA) is the major constituent of Salvia miltiorrhiza Bunge and has been shown to display anti-inflammatory effects. The aim of the current study was to investigate the effects of Tan-ⅡA on ALI.Methods: A murine model of lipopolysaccharide(LPS)-induced ALI was used. The lungs and serum samples of mice were extracted at 3 days after treatment. ALI-induced inflammatory damages were confirmed from cytokine detections and histomorphology observations. Effects of Tan-ⅡA were investigated using in vivo and in vitro ALI models. Tan-ⅡA mechanisms were investigated by performing Western blot and flow cytometry experiments. A wound-healing assay was performed to confirm the Tan-ⅡA function.Results: The cytokine storm induced by LPS treatment was detected at 3 days after LPS treatment, and alveolar epithelial damage and lymphocyte aggregation were observed. Tan-ⅡA treatment attenuated the LPS-induced inflammation and reduced the levels of inflammatory cytokines released not only by inhibiting neutrophils, but also by macrophage. Moreover, we found that macrophage activation and polarization after LPS treatment were abrogated after applying the Tan-ⅡA treatment. An in vitro assay also confirmed that including the Tan-ⅡA supplement increased the relative amount of the M2 subtype and decreased that of M1. Rebalanced macrophages and Tan-ⅡA inhibited activations of the nuclear factor-κB and hypoxia-inducible factor pathways. Including Tan-ⅡA and macrophages also improved alveolar epithelial repair by regulating macrophage polarization.Conclusion: This study found that while an LPS-induced cytokine storm exacerbated ALI, including Tan-ⅡA could prevent ALI-induced inflammation and improve the alveolar epithelial repair, and do so by regulating macrophage polarization.
基金supported by the National Natural Science Foundation of China(Nos.60772047and61101152)the National Science & Technology Pillar Program during the Eleventh Five-year Plan Period(No.2006BAK08B07)the Chuanxin Foundation from Tsinghua University(No.110107001)
文摘Sparse Representation based Classification （SRC） has emerged as a new paradigm for solving recognition problems. This paper presents a constraint sampling feature extraction method that improves the SRC recognition rate. The method combines texture and shape features to significantly improve the recognition rate. Tests show that the combined constraint sampling and facial alignment achieves very high recognition accuracy on both the AR face database （99.52%） and the CAS-PEAL face database （99.54%）.
基金a European Marie Curie International Incoming Fellowship(326847 and 912847)a Special Foundation for Talents of Northwest A&F University(Z111021801)two Key Research and Development Plans of Shaanxi Province(2019NY-172 and 2018030).
文摘Bionic inspiration from human thumb and index finger was the drive to design a high-performance two-finger dexterous hand.The size of each phalanx and the motion range of each joint in the human thumb and index finger were summarized,and the features of three grasping patterns were described in detail.Subsequently,a two-finger dexterous bionic hand with 6 Degrees of Freedom(DoFs)was developed.Both the mechanical thumb and index finger were made up of three rigid phalanx links and three mechanical rotation joints.Some grasp-release tests validated that the bionic hand can perform three grasping patterns:power grasp,precision pinch and lateral pinch.The grasping success rates were high under the following cases:(1)when power grasping was used to grasp a ring with external diameter 20 mm-140 mm,a cylinder with mass<500 g,or objects with cylinder,sphere or ellipsoid shape;(2)when the precision pinch was used to grasp thin or small objects;(3)when the lateral pinch was used to grasp low length-to-width ratio of objects.The work provided a method for developing two-fimger bionic hand with three grasping patterns,and further revealed the linkage between the difference in fimger structure and size and the hand manipulation dexterity.
基金General program of National Natural Science Foundation of China(Grant No.81573941)Open fund project for first-class disciplines of traditional Chinese Medicine(Grant No.2021ZYX20)Graduate research innovation project of Hunan Province(Grant No.CX20200798)。
文摘Cerebral ischemia(CI)is the world’s second-largest lethal disease,with a high recurrence and teratogenic rate.Traditional Chinese medicine(TCM)YQHX(a pharmaceutical preparation from herbs)has a certain effect in the clinical treatment of CI,while its underlying mechanism remains largely undetermined.To explore the potential mechanism,we used network pharmacology and molecular docking in the present study.TCMSP and CNKI databases were used to explore the active ingredients of YQHX;the Pharmmapper database was used to get the ingredient targets;the OMIM,GeneCards,and DisGeNET databases were used to obtain the disease targets;the Venn diagram was used to obtain the intersection targets,the Cytoscape was used to visualize results and plug-in MCODE to obtain core targets;the Metascape database was used to perform GO and KEGG pathway enrichment analyses on core targets.The top 20 KEGG pathway enrichment pathways were used to construct the“ingredient-target-pathway”network by Cytoscape;the top 10 ingredients and the top five protein targets were used for molecular docking with AutoDock Vina software,and PyMoL and Ligplus software were used to visualize the results.A total of 83 active ingredients were screened from YQHX.Moreover,432 corresponding targets,2005 disease-related targets,and 140 drug-disease intersection targets were obtained.GO biological function and KEGG pathway enrichment analyses yielded 507 biological function entries and 141 signaling pathways.KEGG pathway enrichment was mainly involved in cell proliferation,adhesion,migration,and other processes.Molecular docking results showed that the key ingredients and core targets screened had a strong binding activity,including EGFR,MAP2K1,and KDR.The combination of miltionone I and miltiodiol was relatively stable.The main biological mechanism of YQHX in the treatment of CI might play a role through the signaling pathway related to the tyrosine kinase receptor,which was also the improvement of the theory of“benefiting qi and activating blood circulation for promoting the production of blood and blood vessels”.
基金This study was supported by grants from National Science Foundation of China （No.81570020）, Science and Technology Development Fund of Pudong New Area （No.PKJ2016-Y49）, Zhejiang Province Public Welfare TechnologyApplication Research Project Foundation （No.2016C33216）, Key Research Project of Educational Commission of Hunan Province （No.16A152）, and Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry.
文摘To the Editor：Asthma poses a serious health problem globally.People of all ages can be affected by this chronic airway disorder that,when uncontrolled,may place severe limits on daily life and is sometimes fatal.The prevalence of asthma is increasing in most countries throughout the world,especially among children.[1]During the past decades,many scientific advances have been made to improve our understanding of asthma and our ability to manage and control it effectively.These advances are mainly reflected by related scientific articles,particularly by those most influential papers.
