为分析不同饲料喂养对黄牛胃中微生物群落结构的影响,分别用芒草单一喂养和农家混合饲料喂养两年的黄牛为实验组和对照组。以瘤胃、蜂巢胃、重瓣胃和皱胃四个胃中的微生物为研究对象,采用原位包埋裂解法和脉冲场电泳（pulsed field gel ...为分析不同饲料喂养对黄牛胃中微生物群落结构的影响,分别用芒草单一喂养和农家混合饲料喂养两年的黄牛为实验组和对照组。以瘤胃、蜂巢胃、重瓣胃和皱胃四个胃中的微生物为研究对象,采用原位包埋裂解法和脉冲场电泳（pulsed field gel electrophoresis,PFGE）提取微生物总DNA,脉冲场电泳（pulsed field gel electrophoresis,PFGE）。使用细菌16S rRNA引物341F/534R及真菌18S rD NA引物NS1/GCFungi进行降落PCR扩增。变性梯度凝胶电泳（denaturing gradient gel electrophoresis,DGGE）对扩增产物进行区分,使用硝酸银染色。电泳扫描结果通过Quantity one软件进行分析,SPSS软件进行数据统计。针对实验组和对照组瘤胃样品共性条带和特性条带测序并比对。结果表明：实验组与对照组细菌群落结构变化较大,UPGAM聚类图上被分为两支,相似性只有0.35。且香农多样性指数及条带丰度都明显少于对照组。真菌DGGE图谱条带差别不大,聚类图上显示实验组四个样品与对照组四个样品相似性在0.43-0.68之间。多样性及条带丰度在实验组与对照组之间差异0.0027-0.5999。测序结果,细菌与数据库中未培养细菌种类较为接近,而真菌中部分与已知菌种接近。芒草单一饲养对牛胃中的微生物群落结构具有极大的影响,对细菌的影响尤为明显。展开更多
Hydrogenated amorphous silicon oxide(a-SiOx:H) is an attractive passivation material to suppress epitaxial growth and reduce the parasitic absorption loss in silicon heterojunction(SHJ) solar cells. In this paper, a-S...Hydrogenated amorphous silicon oxide(a-SiOx:H) is an attractive passivation material to suppress epitaxial growth and reduce the parasitic absorption loss in silicon heterojunction(SHJ) solar cells. In this paper, a-SiOx:H layers on different orientated c-Si substrates are fabricated. An optimal effective lifetime(τ(eff)) of 4743 μs and corresponding implied opencircuit voltage(iV(oc)) of 724 mV are obtained on〈100〉-orientated c-Si wafers. While τ(eff) of 2429 μs and iV_(oc) of 699 mV are achieved on 111-orientated substrate. The FTIR and XPS results indicate that the a-SiOx:H network consists of SiOx(Si-rich), Si–OH, Si–O–SiHx, SiO2 ≡ Si–Si, and O3 ≡ Si–Si. A passivation evolution mechanism is proposed to explain the different passivation results on different c-Si wafers. By modulating the a-SiOx:H layer, the planar silicon heterojunction solar cell can achieve an efficiency of 18.15%.展开更多
基金Supported by National Natural Science Foundation of China(No.31072141)Hunan Provincial Natural Science Foundation of China(No.13JJ3054)The Construct Program of the Key Discipline of Basic Medicine in Hunan Province
文摘为分析不同饲料喂养对黄牛胃中微生物群落结构的影响,分别用芒草单一喂养和农家混合饲料喂养两年的黄牛为实验组和对照组。以瘤胃、蜂巢胃、重瓣胃和皱胃四个胃中的微生物为研究对象,采用原位包埋裂解法和脉冲场电泳（pulsed field gel electrophoresis,PFGE）提取微生物总DNA,脉冲场电泳（pulsed field gel electrophoresis,PFGE）。使用细菌16S rRNA引物341F/534R及真菌18S rD NA引物NS1/GCFungi进行降落PCR扩增。变性梯度凝胶电泳（denaturing gradient gel electrophoresis,DGGE）对扩增产物进行区分,使用硝酸银染色。电泳扫描结果通过Quantity one软件进行分析,SPSS软件进行数据统计。针对实验组和对照组瘤胃样品共性条带和特性条带测序并比对。结果表明：实验组与对照组细菌群落结构变化较大,UPGAM聚类图上被分为两支,相似性只有0.35。且香农多样性指数及条带丰度都明显少于对照组。真菌DGGE图谱条带差别不大,聚类图上显示实验组四个样品与对照组四个样品相似性在0.43-0.68之间。多样性及条带丰度在实验组与对照组之间差异0.0027-0.5999。测序结果,细菌与数据库中未培养细菌种类较为接近,而真菌中部分与已知菌种接近。芒草单一饲养对牛胃中的微生物群落结构具有极大的影响,对细菌的影响尤为明显。
基金Project supported by the National Key Research and Deveopment Program of China(Grant No.2018YFB1500402)the National Natural Science Foundation of China(Grant Nos.61674084 and 61874167)+5 种基金the Fundamental Research Funds for Central Universities,Chinathe Natural Science Foundation of Tianjin City,China(Grant No.17JCYBJC41400)the Open Fund of the Key Laboratory of Optical Information Science&Technology of Ministry of Education of China(Grant No.2017KFKT014)the 111 Project,China(Grant No.B16027)the International Cooperation Base,China(Grant No.2016D01025)Tianjin International Joint Research and Development Center,China。
文摘Hydrogenated amorphous silicon oxide(a-SiOx:H) is an attractive passivation material to suppress epitaxial growth and reduce the parasitic absorption loss in silicon heterojunction(SHJ) solar cells. In this paper, a-SiOx:H layers on different orientated c-Si substrates are fabricated. An optimal effective lifetime(τ(eff)) of 4743 μs and corresponding implied opencircuit voltage(iV(oc)) of 724 mV are obtained on〈100〉-orientated c-Si wafers. While τ(eff) of 2429 μs and iV_(oc) of 699 mV are achieved on 111-orientated substrate. The FTIR and XPS results indicate that the a-SiOx:H network consists of SiOx(Si-rich), Si–OH, Si–O–SiHx, SiO2 ≡ Si–Si, and O3 ≡ Si–Si. A passivation evolution mechanism is proposed to explain the different passivation results on different c-Si wafers. By modulating the a-SiOx:H layer, the planar silicon heterojunction solar cell can achieve an efficiency of 18.15%.
