作为一种广谱表达的细胞粘附分子,Ⅰ型跨膜糖蛋白CD44(cluster of differentiation 44)参与细胞增殖、分化、迁移,血管生成等生物学过程,对于介导细胞信号转导,调节组织稳态等功能具有关键作用.特别地,CD44-选择素、CD44-透明质酸相互...作为一种广谱表达的细胞粘附分子,Ⅰ型跨膜糖蛋白CD44(cluster of differentiation 44)参与细胞增殖、分化、迁移,血管生成等生物学过程,对于介导细胞信号转导,调节组织稳态等功能具有关键作用.特别地,CD44-选择素、CD44-透明质酸相互作用介导的细胞粘附动力学在经典炎症反应、肿瘤转移或组织特异的肝脏免疫中具有重要作用.该综述分别从细胞层次粘附动力学、二维与三维条件下的分子层次反应动力学、原子层次微观结构以及胞内信号转导通路等方面综述了CD44-选择素、CD44-透明质酸相互作用的研究进展及尚待回答的生物力学问题.力学、物理因素对生命活动的不可或缺性逐渐被研究者们接受,力学医学、力学免疫学、力学组学等新概念相继提出.生理、病理条件下,CD44-配体相互作用介导的细胞粘附必将受到血流剪切、基底硬度等力学、物理微环境的调控,但是其调控机制还远不清楚.基于此,本文就CD44-配体相互作用相关的未来研究方向做出展望,主要包括:力学、物理因素如何调控CD44-配体相互作用介导的细胞粘附动力学及其内在机制;CD44-配体相互作用反应动力学的力学调控规律及结构基础是什么;以及力学作用下CD44-配体相互作用原子层次的微观结构如何发生动态演化.本文可为深入理解CD44-配体相互作用的生物学功能及其结构功能关系提供线索.展开更多
Molecular dynamics simulation(MDS)is a powerful technology for investigating evolution dynamics of target proteins,and it is used widely in various fields from materials to biology.This mini-review introduced the prin...Molecular dynamics simulation(MDS)is a powerful technology for investigating evolution dynamics of target proteins,and it is used widely in various fields from materials to biology.This mini-review introduced the principles,main preforming procedures,and advances of MDS,as well as its applications on the studies of conformational and allosteric dynamics of proteins especially on that of the mechanosensitive integrins.Future perspectives were also proposed.This review could provide clues in understanding the potentiality of MD simulations in structure–function relationship investigation of biological proteins.展开更多
文摘作为一种广谱表达的细胞粘附分子,Ⅰ型跨膜糖蛋白CD44(cluster of differentiation 44)参与细胞增殖、分化、迁移,血管生成等生物学过程,对于介导细胞信号转导,调节组织稳态等功能具有关键作用.特别地,CD44-选择素、CD44-透明质酸相互作用介导的细胞粘附动力学在经典炎症反应、肿瘤转移或组织特异的肝脏免疫中具有重要作用.该综述分别从细胞层次粘附动力学、二维与三维条件下的分子层次反应动力学、原子层次微观结构以及胞内信号转导通路等方面综述了CD44-选择素、CD44-透明质酸相互作用的研究进展及尚待回答的生物力学问题.力学、物理因素对生命活动的不可或缺性逐渐被研究者们接受,力学医学、力学免疫学、力学组学等新概念相继提出.生理、病理条件下,CD44-配体相互作用介导的细胞粘附必将受到血流剪切、基底硬度等力学、物理微环境的调控,但是其调控机制还远不清楚.基于此,本文就CD44-配体相互作用相关的未来研究方向做出展望,主要包括:力学、物理因素如何调控CD44-配体相互作用介导的细胞粘附动力学及其内在机制;CD44-配体相互作用反应动力学的力学调控规律及结构基础是什么;以及力学作用下CD44-配体相互作用原子层次的微观结构如何发生动态演化.本文可为深入理解CD44-配体相互作用的生物学功能及其结构功能关系提供线索.
基金Project supported by the National Key Research and Development Program of China(Grant No.2016YFA0501601)the National Natural Science Foundation of China(Grant Nos.91642203,31627804,and 11972042)+2 种基金the Frontier Science Key Project of the Chinese Academy of Sciences(Grant No.QYZDJ-SSWJSC018)the Scientific Instrument Developing Project of the Chinese Academy of Sciences(Grant No.GJJSTU20190005)the Strategic Priority Research Program of the Chinese Academy of Sciences(Grant No.XDB22040101)。
文摘Molecular dynamics simulation(MDS)is a powerful technology for investigating evolution dynamics of target proteins,and it is used widely in various fields from materials to biology.This mini-review introduced the principles,main preforming procedures,and advances of MDS,as well as its applications on the studies of conformational and allosteric dynamics of proteins especially on that of the mechanosensitive integrins.Future perspectives were also proposed.This review could provide clues in understanding the potentiality of MD simulations in structure–function relationship investigation of biological proteins.
