Objective To develop a nationally acceptable blood pressure(BP)reference standards for the diagnosis and evaluation of hypertension in children and adolescents in China.Methods The current study was based on the data ...Objective To develop a nationally acceptable blood pressure(BP)reference standards for the diagnosis and evaluation of hypertension in children and adolescents in China.Methods The current study was based on the data from eleven large-scale cross-sectional BP surveys in China's Mainland since 2001,the survey sites were representative of national geographical distribution including four municipalities(Beijing,Shanghai,Tianjin,Chongqing)and seven provinces(Hunan,Liaoning,Hebei,Zhejiang,Guangxi,Xinqiang and Guangdong).The surveys which met the following selection criteria were involved to form the reference population sample and pooled database:1)Subjects were Han nationality and their ages were from 3 to 18 years old;2)BP levels were measured by auscultation using standard sphygmomanometer and recorded as Korotkoff phase 1(SBP),Korotkoff phase 4(DBP-K4)and/or Korotkoff phase 5(DBP-K5);3)All surveyors were trained before investigations and standard quality control was implemented throughout the BP measuring process;4)Basic variables,except BPs,were at least included but not limited to age,sex,nationality,height and weight.Totally 112 227 subjects(56 912 males accounting for 50.7%)were enrolled in the reference population sample in this study.SPSS 13.0 software was used to do the descriptive statistical analysis.Centile curves for SBP,DBP-K4 and DBP-K5 were drawn by sex using LMS method.Z scores of Height and BMI were calculated based on the data from the Chinese National Survey on Constitution and Health(CNSCH)in schoolchildren in 2005 to evaluate the nutrition status and development level of the reference population.Results The reference population had an optimal representation of Chinese Han children and adolescents.The resulting curves provided specific cut off points based on age and sex for the diagnosis of high normal BP,hypertension and severe hypertension,respectively.Given the best approach for blood pressure measurement in children and adolescents remained controversial,especially on the choice of K4 or K5 for diastolic blood pressure.So in present study we showed DBP reference standards with K4 and K5,respectively,that is,two sets of SBP/DBP-K4 and SBP/DBP-K5 were developed for the flexible usage in practice.The proposed reference standards of SBP/DBP-K4 were similar to the US fourth report on the diagnosis,evaluation,and treatment of high blood pressure in children and adolescents,with only exception of the age groups of preschool children and late adolescence.Cut off points for hypertension using DBP-K5 were significantly lower than the US standards.Conclusions The proposed reference standards,which were firstly developed based on the most recently pooled national data and in line with the growth and development characteristics of Chinese children and adolescents,should help for the early screening,diagnosis and evaluation of hypertension in children and adolescents,and were also recommended to be used in national comparisons of prevalence of hypertension.展开更多
目的探讨兰索拉唑对幽门螺杆菌(helicobacter pylori,Hp)感染的胃上皮细胞的体外抗炎效应。方法体外培养胃上皮细胞系AGS,加入兰索拉唑和培养的Hp感染不同时间。ELISA检测IL-1β和IL-8的产生,Western blot检测NF-κB p 65亚基的核转位...目的探讨兰索拉唑对幽门螺杆菌(helicobacter pylori,Hp)感染的胃上皮细胞的体外抗炎效应。方法体外培养胃上皮细胞系AGS,加入兰索拉唑和培养的Hp感染不同时间。ELISA检测IL-1β和IL-8的产生,Western blot检测NF-κB p 65亚基的核转位以及红素氧合酶1(Heme oxygenase-1,HO-1)蛋白表达水平。结果兰索拉唑孵育8 h后,能显著抑制Hp诱导的IL-1β和IL-8。同时,兰索拉唑能抑制NF-κB p 65亚基的核转位,并能上调HO-1蛋白的表达。结论兰索拉唑可能通过抑制NF-κB的激活,上调HO-1的表达而发挥对胃上皮细胞的抗炎作用。展开更多
目的分析并探索剪切波弹性成像技术在冻结肩疾病中诊断价值。方法采用回顾性病例对照研究方法,选择2019年1月至2020年1月在宝鸡高新医院就诊的临床诊断为冻结肩患者80例(病例组),以及同期无肩关节疼痛的并行肩关节超声检查、排除冻结肩...目的分析并探索剪切波弹性成像技术在冻结肩疾病中诊断价值。方法采用回顾性病例对照研究方法,选择2019年1月至2020年1月在宝鸡高新医院就诊的临床诊断为冻结肩患者80例(病例组),以及同期无肩关节疼痛的并行肩关节超声检查、排除冻结肩的患者356例,采用倾向性得分匹配方法得到80例患者(对照组)。病例组中男性23例,女性57例;年龄40~70岁,平均年龄54.89岁;体质量指数19.68~27.78 kg/m^(2),平均体质量指数24.20 kg/m^(2);有慢性病(包括糖尿病、高血压、冠心病在内的一种或几种)34例。对照组中其中男性23例,女性57例;年龄37~73岁,平均年龄53.17岁;体质量指数17.24~28.33kg/m^(2),平均体质量指数22.80kg/m^(2);有慢性病(包括糖尿病、高血压、冠心病在内的一种或几种)33例。采用单因素及多因素分析影响冻结肩发病的危险因素,并选择其中有统计学意义的因素进行受试者操作特性(ROC)曲线分析。结果病例组和对照组患者的临床资料具有可比性(P=0.727、0.724、1.000、0.873)。病例组肱二头肌长头肌腱、冈上肌腱、冈下肌腱、肩胛下肌腱、喙肱韧带和盂肱关节囊弹性模量均显著高于对照组(102.17kPa vs 86.20 kPa、124.96 kPa vs 84.98 kPa、168.28 kPa vs 100.29 kPa、91.67 kPa vs 72.07 kPa、118.76 kPa vs 102.58 kPa、66.64 kPa vs 34.66 kPa)(P<0.05)。病例组冈上肌腱(χ^(2)_(单因素)=16.450,P<0.001;χ^(2)_(多因素)=13.438,P<0.001)、冈下肌腱(χ^(2)_(单因素)=8.829,P=0.003;χ^(2)_(多因素)=6.829,P=0.013)和盂肱关节囊(χ^(2)_(单因素)=21.249,P<0.001;χ^(2)_(多因素)=18.381,P<0.001)的弹性模量值在单因素和多因素分析中均明显高于其对照组数值,而肱二头肌长头肌腱、肩胛下肌腱和喙肱韧带的弹性模量值在两组间未见明显差异。对冈上肌腱诊断、冈下肌腱诊断和盂肱关节囊诊断进行ROC曲线分析结果显示,冈上肌腱诊断[曲线下面积(AUC)_(网上肌健诊断)=0.689]、冈下肌腱诊断(AUC_(冈下肌健诊断)=0.621)、盂肱关节囊诊断(AUC_(盂陆关节囊诊断)=0.721)和联合诊断(AUC_(联合诊断)=0.800)均具有良好的诊断价值,但联合诊断的诊断效能更加优于各单项指标。结论超声剪切波弹性成像技术是诊断冻结肩的有力辅助手段,其中冈上肌腱、冈下肌腱及盂肱关节囊弹性模量数值均具有良好的诊断效能,三者联合诊断则具有更好的临床应用价值。展开更多
文摘Objective To develop a nationally acceptable blood pressure(BP)reference standards for the diagnosis and evaluation of hypertension in children and adolescents in China.Methods The current study was based on the data from eleven large-scale cross-sectional BP surveys in China's Mainland since 2001,the survey sites were representative of national geographical distribution including four municipalities(Beijing,Shanghai,Tianjin,Chongqing)and seven provinces(Hunan,Liaoning,Hebei,Zhejiang,Guangxi,Xinqiang and Guangdong).The surveys which met the following selection criteria were involved to form the reference population sample and pooled database:1)Subjects were Han nationality and their ages were from 3 to 18 years old;2)BP levels were measured by auscultation using standard sphygmomanometer and recorded as Korotkoff phase 1(SBP),Korotkoff phase 4(DBP-K4)and/or Korotkoff phase 5(DBP-K5);3)All surveyors were trained before investigations and standard quality control was implemented throughout the BP measuring process;4)Basic variables,except BPs,were at least included but not limited to age,sex,nationality,height and weight.Totally 112 227 subjects(56 912 males accounting for 50.7%)were enrolled in the reference population sample in this study.SPSS 13.0 software was used to do the descriptive statistical analysis.Centile curves for SBP,DBP-K4 and DBP-K5 were drawn by sex using LMS method.Z scores of Height and BMI were calculated based on the data from the Chinese National Survey on Constitution and Health(CNSCH)in schoolchildren in 2005 to evaluate the nutrition status and development level of the reference population.Results The reference population had an optimal representation of Chinese Han children and adolescents.The resulting curves provided specific cut off points based on age and sex for the diagnosis of high normal BP,hypertension and severe hypertension,respectively.Given the best approach for blood pressure measurement in children and adolescents remained controversial,especially on the choice of K4 or K5 for diastolic blood pressure.So in present study we showed DBP reference standards with K4 and K5,respectively,that is,two sets of SBP/DBP-K4 and SBP/DBP-K5 were developed for the flexible usage in practice.The proposed reference standards of SBP/DBP-K4 were similar to the US fourth report on the diagnosis,evaluation,and treatment of high blood pressure in children and adolescents,with only exception of the age groups of preschool children and late adolescence.Cut off points for hypertension using DBP-K5 were significantly lower than the US standards.Conclusions The proposed reference standards,which were firstly developed based on the most recently pooled national data and in line with the growth and development characteristics of Chinese children and adolescents,should help for the early screening,diagnosis and evaluation of hypertension in children and adolescents,and were also recommended to be used in national comparisons of prevalence of hypertension.
文摘目的探讨兰索拉唑对幽门螺杆菌(helicobacter pylori,Hp)感染的胃上皮细胞的体外抗炎效应。方法体外培养胃上皮细胞系AGS,加入兰索拉唑和培养的Hp感染不同时间。ELISA检测IL-1β和IL-8的产生,Western blot检测NF-κB p 65亚基的核转位以及红素氧合酶1(Heme oxygenase-1,HO-1)蛋白表达水平。结果兰索拉唑孵育8 h后,能显著抑制Hp诱导的IL-1β和IL-8。同时,兰索拉唑能抑制NF-κB p 65亚基的核转位,并能上调HO-1蛋白的表达。结论兰索拉唑可能通过抑制NF-κB的激活,上调HO-1的表达而发挥对胃上皮细胞的抗炎作用。
文摘目的分析并探索剪切波弹性成像技术在冻结肩疾病中诊断价值。方法采用回顾性病例对照研究方法,选择2019年1月至2020年1月在宝鸡高新医院就诊的临床诊断为冻结肩患者80例(病例组),以及同期无肩关节疼痛的并行肩关节超声检查、排除冻结肩的患者356例,采用倾向性得分匹配方法得到80例患者(对照组)。病例组中男性23例,女性57例;年龄40~70岁,平均年龄54.89岁;体质量指数19.68~27.78 kg/m^(2),平均体质量指数24.20 kg/m^(2);有慢性病(包括糖尿病、高血压、冠心病在内的一种或几种)34例。对照组中其中男性23例,女性57例;年龄37~73岁,平均年龄53.17岁;体质量指数17.24~28.33kg/m^(2),平均体质量指数22.80kg/m^(2);有慢性病(包括糖尿病、高血压、冠心病在内的一种或几种)33例。采用单因素及多因素分析影响冻结肩发病的危险因素,并选择其中有统计学意义的因素进行受试者操作特性(ROC)曲线分析。结果病例组和对照组患者的临床资料具有可比性(P=0.727、0.724、1.000、0.873)。病例组肱二头肌长头肌腱、冈上肌腱、冈下肌腱、肩胛下肌腱、喙肱韧带和盂肱关节囊弹性模量均显著高于对照组(102.17kPa vs 86.20 kPa、124.96 kPa vs 84.98 kPa、168.28 kPa vs 100.29 kPa、91.67 kPa vs 72.07 kPa、118.76 kPa vs 102.58 kPa、66.64 kPa vs 34.66 kPa)(P<0.05)。病例组冈上肌腱(χ^(2)_(单因素)=16.450,P<0.001;χ^(2)_(多因素)=13.438,P<0.001)、冈下肌腱(χ^(2)_(单因素)=8.829,P=0.003;χ^(2)_(多因素)=6.829,P=0.013)和盂肱关节囊(χ^(2)_(单因素)=21.249,P<0.001;χ^(2)_(多因素)=18.381,P<0.001)的弹性模量值在单因素和多因素分析中均明显高于其对照组数值,而肱二头肌长头肌腱、肩胛下肌腱和喙肱韧带的弹性模量值在两组间未见明显差异。对冈上肌腱诊断、冈下肌腱诊断和盂肱关节囊诊断进行ROC曲线分析结果显示,冈上肌腱诊断[曲线下面积(AUC)_(网上肌健诊断)=0.689]、冈下肌腱诊断(AUC_(冈下肌健诊断)=0.621)、盂肱关节囊诊断(AUC_(盂陆关节囊诊断)=0.721)和联合诊断(AUC_(联合诊断)=0.800)均具有良好的诊断价值,但联合诊断的诊断效能更加优于各单项指标。结论超声剪切波弹性成像技术是诊断冻结肩的有力辅助手段,其中冈上肌腱、冈下肌腱及盂肱关节囊弹性模量数值均具有良好的诊断效能,三者联合诊断则具有更好的临床应用价值。
