体外培养的胎肝细胞半同系移植效果的研究

以Dexter体系成功地建立了小鼠胎肝细胞体外长期培养技术。培养的胎肝造血干细胞(CFU-S)有旺盛的增殖力。半同系移植研究证实,5×10~6个培养细胞(含780～1200CFU-S)可使9.5Gy γ-线照射的受体小鼠30d和60d存活率达80％和74％。1个月造血功能恢复。无移植物抗宿主病(GVHD)征象,并在受体内形成了稳定的嵌合体。说明体外培养的胎肝细胞能有效地重建造血。观察小鼠移植后14.5个月的远期疗效,其造血机能健全,体内CFU-S的移植效力与正常相似。然而CFU-S的再生速率和自我更新力均较正常同龄鼠低,揭示出长期增殖状态的造血干细胞功能上的变化。

小鼠CFU-GEMM性能的研究

本文对小鼠多能造血祖细胞(CFU-GEMM)进行了集落细胞组成、克隆线性关系、细胞周期活动及再植能力的观察;并在胎肝体外长期培养中观察了其数量的动态变化。发现:CFU-GEMM具有一定的增殖和自我更新能力,可多向分化,其中的细胞多数处于相对静止状态,是一群性能非常接近于CFU-S的多能祖细胞。胎肝体外长期培养中,CFU-GEMM与CFU-S数量的动态变化趋势也是一致的。这一结果表明:在人类及其它大动物目前还不能测定CFU-S的情况下,CFU-GEMM是一类可以反映造血干细胞池性能及动态变化的细胞。

STUDIES ON THE PROPERTY AND TRANSPLANTATION OF HAEMOPOIETIC STEM CELLS FROM PERIPHERAL BLOOD

Comparative studies of the properties of murine haemopoietic stem cells from differentsources revealed that the peripheral haemopoietic stem cell is relatively weaker than the haemo-poietic stem cell from bone marrow in promoting the recovery of hemopoiesis in the irradiatedanimals. This is due to the heterogenity of stem cell population in which some aged cellsub-populations aro co-existing. The modified potential in proliferation and differentiation ofhaemopoietic stem cells in the peripheral blood seems to be irreversible under normal physio-logical conditons.In a preliminary experiment, the use of an anti-thymocyte immunoglob-ulin to eliminate immunocompetent cells proved effective in reducing the severity and incidenceof secondary diseases and in increasing the number of survivors of lethally irradiated semi-isologous mice after transplantation of parental peripheral mononuclear cells.