This study investigated the role of glucose in the biogenesis of high-density lipoprotein cholesterol(HDL-C).Mouse primary peritoneal macrophages were harvested and maintained in Dulbecco’s modified Eagle’s medium(D...This study investigated the role of glucose in the biogenesis of high-density lipoprotein cholesterol(HDL-C).Mouse primary peritoneal macrophages were harvested and maintained in Dulbecco’s modified Eagle’s medium(DMEM) containing glucose of various concentrations.The cells were divided into 3 groups in terms of different glucose concentrations in the cultures:Control group(5.6 mmol/L glucose),high glucose concentration groups(16.7 mmol/L and 30 mmol/L glucose).ATP-binding cassette transporter A1(ABCA1) mRNA expression in the macrophages was detected by semi-quantitative RT-PCR 24,48 and 72 h after glucose treatment.The results showed that ABCA1 mRNA expression in the 16.7 mmol/L glucose group was not significantly different from that in the control group at all testing time points(P>0.05 for each).In the 30 mmol/L glucose group,macrophage ABCA1 mRNA expression was not changed significantly at 24 h(P=0.14),but was substantially decreased by 40.4% at 48 h(P=0.009) and by 48.1% at 72 h(P=0.015) as compared with that in the control group.It was concluded that ABCA1 is of vital importance for HDL-C biogenesis.High glucose may hamper HDL-C biogenesis by decreasing ABCA1 expression,which contributes to low HDL-C level in diabetes.展开更多
2023年12月11日,美国糖尿病协会(American Diabetes Association,ADA)正式颁布了《2024年糖尿病诊疗标准(Standards of Care in Diabetes—2024)》。长期以来,ADA糖尿病诊疗标准一直被视为国际糖尿病领域的风向标,对于各国糖尿病诊疗有...2023年12月11日,美国糖尿病协会(American Diabetes Association,ADA)正式颁布了《2024年糖尿病诊疗标准(Standards of Care in Diabetes—2024)》。长期以来,ADA糖尿病诊疗标准一直被视为国际糖尿病领域的风向标,对于各国糖尿病诊疗有很大的参考价值。《2024年糖尿病诊疗标准》继续强调了“以降糖为中心”向“以患者为中心”诊疗模式的转变,突出强调了“合理控糖、管理体重、改善心肾预后”作为糖尿病患者综合管理的重要性,同时继续肯定了胰高血糖素样肽-1受体激动剂等新型降糖药物以及减重代谢手术在2型糖尿病综合管理方面的优势地位。展开更多
基金supported by a grant from the Scientific Research Foundation for the Returned Overseas Chinese Scholars by the State Education Ministry of China (No.2005383-6144)
文摘This study investigated the role of glucose in the biogenesis of high-density lipoprotein cholesterol(HDL-C).Mouse primary peritoneal macrophages were harvested and maintained in Dulbecco’s modified Eagle’s medium(DMEM) containing glucose of various concentrations.The cells were divided into 3 groups in terms of different glucose concentrations in the cultures:Control group(5.6 mmol/L glucose),high glucose concentration groups(16.7 mmol/L and 30 mmol/L glucose).ATP-binding cassette transporter A1(ABCA1) mRNA expression in the macrophages was detected by semi-quantitative RT-PCR 24,48 and 72 h after glucose treatment.The results showed that ABCA1 mRNA expression in the 16.7 mmol/L glucose group was not significantly different from that in the control group at all testing time points(P>0.05 for each).In the 30 mmol/L glucose group,macrophage ABCA1 mRNA expression was not changed significantly at 24 h(P=0.14),but was substantially decreased by 40.4% at 48 h(P=0.009) and by 48.1% at 72 h(P=0.015) as compared with that in the control group.It was concluded that ABCA1 is of vital importance for HDL-C biogenesis.High glucose may hamper HDL-C biogenesis by decreasing ABCA1 expression,which contributes to low HDL-C level in diabetes.
文摘2023年12月11日,美国糖尿病协会(American Diabetes Association,ADA)正式颁布了《2024年糖尿病诊疗标准(Standards of Care in Diabetes—2024)》。长期以来,ADA糖尿病诊疗标准一直被视为国际糖尿病领域的风向标,对于各国糖尿病诊疗有很大的参考价值。《2024年糖尿病诊疗标准》继续强调了“以降糖为中心”向“以患者为中心”诊疗模式的转变,突出强调了“合理控糖、管理体重、改善心肾预后”作为糖尿病患者综合管理的重要性,同时继续肯定了胰高血糖素样肽-1受体激动剂等新型降糖药物以及减重代谢手术在2型糖尿病综合管理方面的优势地位。
