目的研究胚胎期氟暴露对斑马鱼骨骼发育的影响。方法将受精3d（3days post fertilization，3dpf）的斑马鱼胚胎暴露于常规养鱼水（对照组，氟离子含量〈0．2mg／L）和含25、50、100mg／L氟化钠（NaF）的养鱼水中，28cc培养5d（8dpf）至...目的研究胚胎期氟暴露对斑马鱼骨骼发育的影响。方法将受精3d（3days post fertilization，3dpf）的斑马鱼胚胎暴露于常规养鱼水（对照组，氟离子含量〈0．2mg／L）和含25、50、100mg／L氟化钠（NaF）的养鱼水中，28cc培养5d（8dpf）至斑马鱼头部骨骼形成，用氟离子选择电极法检测斑马鱼胚胎整体氟含量。重新制作氟暴露模型，分别为对照（0．0mg／L）组，低剂量暴露（0．5、1．0、4．0mg／L）组和高剂量暴露（50．0、100．0mg／L）组，计算各组斑马鱼胚胎存活率；在40倍胚胎镜下观察斑马鱼胚胎大体形态；采用茜素红染色法对斑马鱼骨骼进行染色，显微镜下观察、数码成像后定量检测骨骼染色区域面积及累积光密度（IOD），用于分析骨质硬化和骨质疏松情况。结果对照组，25、50、100mg／L染氟组斑马鱼胚胎整体氟含量分别为（0．32±0．01）、（0．63±0．01）、（0．86±0．02）、（1．21±0．01）μg／150条。与对照组比较，各染氟组斑马鱼胚胎整体氟含量明显增加（P均〈0．05），且有一定的剂量反应关系。重新制作氟暴露模型后，对照组和各染氟组的斑马鱼胚胎存活率分别为96．67％、96．67％、96．67％、98．33％、98．33％和98-33％，组间比较差异无统计学意义（x2=7．309，P〉0．05）；胚胎镜下各染氟组斑马鱼胚胎脊柱均未出现明显畸形；斑马鱼颅骨茜素红染色面积对照组为84380．51±11711．41．低剂量暴露（0．5、1．0、4．0mg／L）组为92592．16±7143．81、92164．85±10136．18和95112．26±13721．91，均高于对照组（P均〈0．05），高剂量暴露（50．0、100．0mg／L）组为67778．92±8597．11和64272．93±9302．57，均低于对照组（P均〈0．05）；斑马鱼颅骨茜素红染色IOD对照组为25094．13±6571．86，低剂量暴露（0．5、1．0、4．0mg／L）组为29754．95±3836．45、28747．36±4677．86和30776．49±5589．63，高于对照组（P均〈0．05），高剂量暴露（50．0、100．0mg／L）组为19263．10±4754．72和18202．58±4897．15，低于对照组（P均〈0．05）。结论低剂量氟暴露使斑马鱼胚胎骨质硬化，高剂量氟暴露使斑马鱼胚胎骨质疏松。展开更多
Objective To observe the effect of arsenic exposure to drinking water on the level of histone 3 lysine 4 trimethylation(H3K4me3)and histone 3 lysine 79 trimethylation(H3K79me3)in peripheral blood leukocytes of human,a...Objective To observe the effect of arsenic exposure to drinking water on the level of histone 3 lysine 4 trimethylation(H3K4me3)and histone 3 lysine 79 trimethylation(H3K79me3)in peripheral blood leukocytes of human,and to analyze the relationship between arsenic exposure and H3K4me3,H3K79me3 modification levels.Methods A cluster sampling survey was carried out in typical endemic arsenicosis areas of Shanxi and Jilin provinces.Two hundred eighty-one local residents with a drinking water age of≥10 years were selected as the survey subjects.According to the arsenic content of drinking water,the tested population was divided into control group(water arsenic content≤0.01 mg/L,60 cases),low water arsenic exposure group(>0.01-0.05 mg/L,61 cases),medium water arsenic exposure group(>0.05-0.10 mg/L,50 cases),and 110 cases of high water arsenic exposure group(>0.10 mg/L).Drinking water samples,immediate urine samples and peripheral blood samples were collected from the subjects.Arsenic content in drinking water and urinary arsenic content were determined via the atomic fluorescence method;histone H3K4me3 and H3K79me3 in peripheral blood leukocytes were determined by dot blot hybridization(Dot Blotting).Results There were no statistically significant differences in age(61.50,60.00,59.50,59.50 years old),different gender(male:20,27,17,40 cases,female:40,34,33,70 cases),body mass index(BMI),smoking and drinking status between the control group,low,medium and high water arsenic exposure groups.Water arsenic content in the control group,low,medium and high water arsenic exposure groups(median:0.005,0.024,0.076,0.150 mg/L),urinary arsenic content(0.011,0.018,0.061,0.134 mg/L),and water arsenic cumulative exposure levels(0.342,1.641,5.273,7.716 mg)were compared between the groups,the differences were statistically significant(H=256.041,88.615,218.610,P<0.01).In the control group,low,medium and high water arsenic exposure groups,the modification levels of H3K4me3(0.100,0.059,0.083,0.083)and H3K79me3(0.049,0.036,0.055,0.052)in peripheral blood leukocytes were not significantly different(H=1.488,2.097,P>0.05).The levels of H3K4me3 and H3K79me3 in peripheral blood leukocytes were positively correlated with water arsenic content,urinary arsenic content,water arsenic cumulative exposure levels(r=0.245,0.221;0.299,0.318;0.149,0.149;P<0.01 or<0.05);there was a positive correlation between H3K4me3 and H3K79me3 modification levels(r=0.811,P<0.01).Conclusion There is a positive correlation between arsenic exposure through drinking water and the levels of H3K4me3 and H3K79me3 in the peripheral blood leukocytes of the population,but it is necessary to expand the sample size for further study.展开更多
文摘目的研究胚胎期氟暴露对斑马鱼骨骼发育的影响。方法将受精3d（3days post fertilization，3dpf）的斑马鱼胚胎暴露于常规养鱼水（对照组，氟离子含量〈0．2mg／L）和含25、50、100mg／L氟化钠（NaF）的养鱼水中，28cc培养5d（8dpf）至斑马鱼头部骨骼形成，用氟离子选择电极法检测斑马鱼胚胎整体氟含量。重新制作氟暴露模型，分别为对照（0．0mg／L）组，低剂量暴露（0．5、1．0、4．0mg／L）组和高剂量暴露（50．0、100．0mg／L）组，计算各组斑马鱼胚胎存活率；在40倍胚胎镜下观察斑马鱼胚胎大体形态；采用茜素红染色法对斑马鱼骨骼进行染色，显微镜下观察、数码成像后定量检测骨骼染色区域面积及累积光密度（IOD），用于分析骨质硬化和骨质疏松情况。结果对照组，25、50、100mg／L染氟组斑马鱼胚胎整体氟含量分别为（0．32±0．01）、（0．63±0．01）、（0．86±0．02）、（1．21±0．01）μg／150条。与对照组比较，各染氟组斑马鱼胚胎整体氟含量明显增加（P均〈0．05），且有一定的剂量反应关系。重新制作氟暴露模型后，对照组和各染氟组的斑马鱼胚胎存活率分别为96．67％、96．67％、96．67％、98．33％、98．33％和98-33％，组间比较差异无统计学意义（x2=7．309，P〉0．05）；胚胎镜下各染氟组斑马鱼胚胎脊柱均未出现明显畸形；斑马鱼颅骨茜素红染色面积对照组为84380．51±11711．41．低剂量暴露（0．5、1．0、4．0mg／L）组为92592．16±7143．81、92164．85±10136．18和95112．26±13721．91，均高于对照组（P均〈0．05），高剂量暴露（50．0、100．0mg／L）组为67778．92±8597．11和64272．93±9302．57，均低于对照组（P均〈0．05）；斑马鱼颅骨茜素红染色IOD对照组为25094．13±6571．86，低剂量暴露（0．5、1．0、4．0mg／L）组为29754．95±3836．45、28747．36±4677．86和30776．49±5589．63，高于对照组（P均〈0．05），高剂量暴露（50．0、100．0mg／L）组为19263．10±4754．72和18202．58±4897．15，低于对照组（P均〈0．05）。结论低剂量氟暴露使斑马鱼胚胎骨质硬化，高剂量氟暴露使斑马鱼胚胎骨质疏松。
文摘Objective To observe the effect of arsenic exposure to drinking water on the level of histone 3 lysine 4 trimethylation(H3K4me3)and histone 3 lysine 79 trimethylation(H3K79me3)in peripheral blood leukocytes of human,and to analyze the relationship between arsenic exposure and H3K4me3,H3K79me3 modification levels.Methods A cluster sampling survey was carried out in typical endemic arsenicosis areas of Shanxi and Jilin provinces.Two hundred eighty-one local residents with a drinking water age of≥10 years were selected as the survey subjects.According to the arsenic content of drinking water,the tested population was divided into control group(water arsenic content≤0.01 mg/L,60 cases),low water arsenic exposure group(>0.01-0.05 mg/L,61 cases),medium water arsenic exposure group(>0.05-0.10 mg/L,50 cases),and 110 cases of high water arsenic exposure group(>0.10 mg/L).Drinking water samples,immediate urine samples and peripheral blood samples were collected from the subjects.Arsenic content in drinking water and urinary arsenic content were determined via the atomic fluorescence method;histone H3K4me3 and H3K79me3 in peripheral blood leukocytes were determined by dot blot hybridization(Dot Blotting).Results There were no statistically significant differences in age(61.50,60.00,59.50,59.50 years old),different gender(male:20,27,17,40 cases,female:40,34,33,70 cases),body mass index(BMI),smoking and drinking status between the control group,low,medium and high water arsenic exposure groups.Water arsenic content in the control group,low,medium and high water arsenic exposure groups(median:0.005,0.024,0.076,0.150 mg/L),urinary arsenic content(0.011,0.018,0.061,0.134 mg/L),and water arsenic cumulative exposure levels(0.342,1.641,5.273,7.716 mg)were compared between the groups,the differences were statistically significant(H=256.041,88.615,218.610,P<0.01).In the control group,low,medium and high water arsenic exposure groups,the modification levels of H3K4me3(0.100,0.059,0.083,0.083)and H3K79me3(0.049,0.036,0.055,0.052)in peripheral blood leukocytes were not significantly different(H=1.488,2.097,P>0.05).The levels of H3K4me3 and H3K79me3 in peripheral blood leukocytes were positively correlated with water arsenic content,urinary arsenic content,water arsenic cumulative exposure levels(r=0.245,0.221;0.299,0.318;0.149,0.149;P<0.01 or<0.05);there was a positive correlation between H3K4me3 and H3K79me3 modification levels(r=0.811,P<0.01).Conclusion There is a positive correlation between arsenic exposure through drinking water and the levels of H3K4me3 and H3K79me3 in the peripheral blood leukocytes of the population,but it is necessary to expand the sample size for further study.
