欧李组培快繁培养基的筛选

以4个欧李优系的嫩枝顶芽及带叶腋的茎段作外植体进行组培快繁,研究不同培养阶段的适宜培养基。结果表明,优系TSO-4的初代培养基为MS+0.5 mg/L 6-BA+0.1 mg/L NAA,继代培养基MS+0.5 mg/L 6-BA+0.1 mg/L NAA,生根培养基1/2MS+0.1 mg/L NAA+0.4 g/L活性炭;优系TSO-5的初代培养基为MS+0.5 mg/L 6-BA+0.1 mg/L NAA,继代培养基MS+0.5 mg/L 6-BA+0.1 mg/L NAA,生根培养基1/2MS+0.05 mg/L NAA;优系TSO-7的初代培养基为MS+0.5 mg/L 6-BA+0.1 mg/L NAA,继代培养基MS+0.3 mg/L 6-BA+0.1 mg/L NAA,生根培养基1/8MS+0.05 mg/L IAA;优系TSO-11的初代培养基为MS+0.5 mg/L 6-BA+0.1 mg/L NAA,继代培养基MS+0.5 mg/L 6-BA+0.1 mg/L NAA,生根培养基1/2MS+0.1 mg/L NAA;4个欧李优系的炼苗基质均为草炭土∶珍珠岩=1∶2。