We examined the effects of calcitonin gene-related pcptide CGRP on the bone resorption stimulated by 1L la after neonatal mouse calvaria were culatured in vitro and bone resorption was determined by measuring the rele...We examined the effects of calcitonin gene-related pcptide CGRP on the bone resorption stimulated by 1L la after neonatal mouse calvaria were culatured in vitro and bone resorption was determined by measuring the release of calcium into the medium after 2 48-hour incubation. CGRP (109-10~8M) blocked completely the ILlx-mediated increase in bone resorption and inhibited the basal bone resorption. In accordence with the effects of calcitonin on osteoclasts, CGRP caused cessation of lamellipodial activity and gradual retraction of lamellipodia when added into the culture medium. Quantitative analysis showed that CGRP (10 6 M) caused a gradual reduction of osteoclastic surface area and convex perium. These data show that CGRP inhibits ILla mediated bone resorption because of its direct regulation of osteoclast activity.展开更多
文摘We examined the effects of calcitonin gene-related pcptide CGRP on the bone resorption stimulated by 1L la after neonatal mouse calvaria were culatured in vitro and bone resorption was determined by measuring the release of calcium into the medium after 2 48-hour incubation. CGRP (109-10~8M) blocked completely the ILlx-mediated increase in bone resorption and inhibited the basal bone resorption. In accordence with the effects of calcitonin on osteoclasts, CGRP caused cessation of lamellipodial activity and gradual retraction of lamellipodia when added into the culture medium. Quantitative analysis showed that CGRP (10 6 M) caused a gradual reduction of osteoclastic surface area and convex perium. These data show that CGRP inhibits ILla mediated bone resorption because of its direct regulation of osteoclast activity.