The BL08U1 A beamline is established as a sophisticated platform at Shanghai Synchrotron Radiation Facility(SSRF), taking advantage of its high spatial resolution(< 30 nm) and high energy resolving power(>10 000...The BL08U1 A beamline is established as a sophisticated platform at Shanghai Synchrotron Radiation Facility(SSRF), taking advantage of its high spatial resolution(< 30 nm) and high energy resolving power(>10 000),for studying properties of solid, liquid, gas, film and other forms of materials at sub-micron scale. In this paper,we present a review on newly implemented techniques, such as total electron yield(TEY), dual energy contrast imaging, nano-CT, soft X-ray excited optical luminance(SXEOL), and coherent diffraction imaging(CDI)under development. Several research cases in nanomaterials, environmental science and biology are presented to demonstrate capabilities of the beamline.展开更多
Spectral bleedthrough （SBT） ratio is dependent on the level of fluorescence intensity in confocal imaging. Precision Frster resonance energy transfer （FRET） algorithm corrects SBT ratio according to fluorescence i...Spectral bleedthrough （SBT） ratio is dependent on the level of fluorescence intensity in confocal imaging. Precision Frster resonance energy transfer （FRET） algorithm corrects SBT ratio according to fluorescence intensity and avoids over-or under-estimation of SBT ratio. In this letter, we propose a new method to accurately measure the FRET efficiency of FRET plasmid in single living cells by combining the calculation of SBT in precision FRET algorithm with E-FRET formulae. We also use this method to measure the FRET efficiency of FRET-Bid, and find that in healthy A549 cells it is about 15%, which is verified by FRET acceptor photobleaching method.展开更多
基金Supported by National Natural Science Foundation of China(Nos.11079050,11290165,11305252 and 11205232)Programs of the Chinese Academy of Sciences(Nos.KJCX2-EW-W09 and KJZD-EW-M03)+2 种基金the Open Research Project of the Large Scientific Facility of the Chinese Academy of Sciences:Study on Self-assembly Technology and Nanometer Array with Ultra-high Densitythe National Natural Science Foundation for Outstanding Young Scientists(No.11225527)the Shanghai Academic Leadership Program(No.13XD1404400)
文摘The BL08U1 A beamline is established as a sophisticated platform at Shanghai Synchrotron Radiation Facility(SSRF), taking advantage of its high spatial resolution(< 30 nm) and high energy resolving power(>10 000),for studying properties of solid, liquid, gas, film and other forms of materials at sub-micron scale. In this paper,we present a review on newly implemented techniques, such as total electron yield(TEY), dual energy contrast imaging, nano-CT, soft X-ray excited optical luminance(SXEOL), and coherent diffraction imaging(CDI)under development. Several research cases in nanomaterials, environmental science and biology are presented to demonstrate capabilities of the beamline.
基金supported by the National Natural Science Foundation of China (Nos. 31071218 and 60627003)the Natural Science Foundation of Guangdong Province (Nos. 8151063101000031 and 9251063101000009)
文摘Spectral bleedthrough （SBT） ratio is dependent on the level of fluorescence intensity in confocal imaging. Precision Frster resonance energy transfer （FRET） algorithm corrects SBT ratio according to fluorescence intensity and avoids over-or under-estimation of SBT ratio. In this letter, we propose a new method to accurately measure the FRET efficiency of FRET plasmid in single living cells by combining the calculation of SBT in precision FRET algorithm with E-FRET formulae. We also use this method to measure the FRET efficiency of FRET-Bid, and find that in healthy A549 cells it is about 15%, which is verified by FRET acceptor photobleaching method.
