Objective: To investigate the possible role of GST-n in esophageal carcinogenesis. Methods: GST-nexpression at mRNA level was studied by in situhybridization (ISH) and at protein level byimmunohistochemistry (IHC). GS...Objective: To investigate the possible role of GST-n in esophageal carcinogenesis. Methods: GST-nexpression at mRNA level was studied by in situhybridization (ISH) and at protein level byimmunohistochemistry (IHC). GST-n expression innormal epithelial cells (NC) of the esophagus,hyperplastic cells (HC), dysplastic cells (DC) from gradeI to III, carcinoma in situ (CIS) and all the cells insquamous cell carcinomas (SCC) were examined in thesame csophageal cancer specimens (n=48) whichprovided a model reflecting the process of esophagealcarcinogenesis. Results: The positive rate of IHCstaining was 87. 5% for NC, 95.3% for HC, 55.9% forDC (grade I: 73.9%, grade II: 47.4%, grade III:41.2%),36.4% for CIS and 45.8% for SCC. The positive rate ofGST-x mRNA expression was 81.2% for NC, 94.4% forHC, 61.9% for DC (grade I: 76.5%, grade II: 61.5%,grade III: 41.7%), 44.4% for CIS and 83.3% for grade ISCC, 30.0% for grade II SCC and 0% for grade IIISCC. There was no statistically sigllificant difference inGST-x expression at the mRNA and the protein level.Conclusion: There is a decreasing tendency of GST-nexpression from dysplasia to CIS and SCC. Thedecrease in GST-n expression is an early event inesophageal carcinogencsis.展开更多
文摘Objective: To investigate the possible role of GST-n in esophageal carcinogenesis. Methods: GST-nexpression at mRNA level was studied by in situhybridization (ISH) and at protein level byimmunohistochemistry (IHC). GST-n expression innormal epithelial cells (NC) of the esophagus,hyperplastic cells (HC), dysplastic cells (DC) from gradeI to III, carcinoma in situ (CIS) and all the cells insquamous cell carcinomas (SCC) were examined in thesame csophageal cancer specimens (n=48) whichprovided a model reflecting the process of esophagealcarcinogenesis. Results: The positive rate of IHCstaining was 87. 5% for NC, 95.3% for HC, 55.9% forDC (grade I: 73.9%, grade II: 47.4%, grade III:41.2%),36.4% for CIS and 45.8% for SCC. The positive rate ofGST-x mRNA expression was 81.2% for NC, 94.4% forHC, 61.9% for DC (grade I: 76.5%, grade II: 61.5%,grade III: 41.7%), 44.4% for CIS and 83.3% for grade ISCC, 30.0% for grade II SCC and 0% for grade IIISCC. There was no statistically sigllificant difference inGST-x expression at the mRNA and the protein level.Conclusion: There is a decreasing tendency of GST-nexpression from dysplasia to CIS and SCC. Thedecrease in GST-n expression is an early event inesophageal carcinogencsis.