目的:观察心理控制源对老年慢性心力衰竭(chronic heart failure,CHF)患者心理健康、生命质量的影响。方法:选取2018年5月至2019年8月,本院收治的老年CHF患者194例,采用多维度健康心理状况心理控制源量表(multidimentional health locus...目的:观察心理控制源对老年慢性心力衰竭(chronic heart failure,CHF)患者心理健康、生命质量的影响。方法:选取2018年5月至2019年8月,本院收治的老年CHF患者194例,采用多维度健康心理状况心理控制源量表(multidimentional health locus of control scale,MHLC)、症状自评量表(symptom Check-list 90,SCL-90)、明尼苏达心力衰竭生命质量问卷(Minnesota living with heart failure questionnaire,MLHFQ)评估患者的心理控制源倾向,心理健康,生命质量。结果:老年CHF患者内控性、机遇得分显著低于常模(P<0.05),权威人士得分显著高于常模(P<0.05);老年CHF患者躯体化、强迫、恐怖、人际敏感、焦虑、抑郁等心理健康因子高于常模评分(P<0.05);老年CHF患者躯体领域、情绪领域与其他领域的生命质量评分高于常模(P<0.05);内控性、权威人士评分与心理健康因子、生命质量因子呈负相关(P<0.05),机遇评分与心理健康因子、生命质量因子呈正相关(P<0.05);结论:老年CHF患者心理控制源倾向于权威人士,其心理健康与生命质量低于常模,心理控制源在一定程度上与患者的心理健康、生命质量密切相关。医护人员可根据CHF患者心理控制源类型给患者制定针对性计划,改善患者心理健康及生命质量。展开更多
To establish a TaqMan-based real-time RT-PCR method for detection of canine distemper virus,the pair of primers and the TaqMan-based probe were designed according to the M protein genes of canine distemper virus strai...To establish a TaqMan-based real-time RT-PCR method for detection of canine distemper virus,the pair of primers and the TaqMan-based probe were designed according to the M protein genes of canine distemper virus strains.The reactive conditions were optimized to improve the sensitivity and specificity of the method.The specificity and sensitivity of the method were high.Canine distemper virus in the thirty-eight samples had been tested by the TaqMan-based real-time RT-PCR,the common RT-PCR and electron microscopy.The sensitivity of the TaqMan-based real-time RT-PCR was higher than the common RT-PCR and electron microscopy.This suggests that the method may be used in clinical diagnosis,epizootic study and laboratory research.展开更多
为建立一套最适合叶用莴苣(Lactuca sativa L.)基因表达量研究的荧光定量PCR技术体系,以叶用莴苣S24为试材,采用SYBR Green Ⅰ法,利用叶用莴苣内参基因的2对引物组合18SR1和18SR2,分别在10μL和20μL反应体系条件下进行扩增反应。结果表...为建立一套最适合叶用莴苣(Lactuca sativa L.)基因表达量研究的荧光定量PCR技术体系,以叶用莴苣S24为试材,采用SYBR Green Ⅰ法,利用叶用莴苣内参基因的2对引物组合18SR1和18SR2,分别在10μL和20μL反应体系条件下进行扩增反应。结果表明,分别在10μL和20μL反应体系条件下内参基因引物组合18SR1的扩增曲线、标准曲线以及溶解曲线等指标都优于内参基因引物组合18SR2;2对内参基因引物组合18SR1和18SR2在10μL反应体系中的扩增曲线、标准曲线以及溶解曲线等指标也均优于20μL反应体系。因此叶用莴苣荧光定量PCR内参基因利用18SR1引物组合,反应体系采用10μL比较合理。展开更多
文摘目的:观察心理控制源对老年慢性心力衰竭(chronic heart failure,CHF)患者心理健康、生命质量的影响。方法:选取2018年5月至2019年8月,本院收治的老年CHF患者194例,采用多维度健康心理状况心理控制源量表(multidimentional health locus of control scale,MHLC)、症状自评量表(symptom Check-list 90,SCL-90)、明尼苏达心力衰竭生命质量问卷(Minnesota living with heart failure questionnaire,MLHFQ)评估患者的心理控制源倾向,心理健康,生命质量。结果:老年CHF患者内控性、机遇得分显著低于常模(P<0.05),权威人士得分显著高于常模(P<0.05);老年CHF患者躯体化、强迫、恐怖、人际敏感、焦虑、抑郁等心理健康因子高于常模评分(P<0.05);老年CHF患者躯体领域、情绪领域与其他领域的生命质量评分高于常模(P<0.05);内控性、权威人士评分与心理健康因子、生命质量因子呈负相关(P<0.05),机遇评分与心理健康因子、生命质量因子呈正相关(P<0.05);结论:老年CHF患者心理控制源倾向于权威人士,其心理健康与生命质量低于常模,心理控制源在一定程度上与患者的心理健康、生命质量密切相关。医护人员可根据CHF患者心理控制源类型给患者制定针对性计划,改善患者心理健康及生命质量。
文摘To establish a TaqMan-based real-time RT-PCR method for detection of canine distemper virus,the pair of primers and the TaqMan-based probe were designed according to the M protein genes of canine distemper virus strains.The reactive conditions were optimized to improve the sensitivity and specificity of the method.The specificity and sensitivity of the method were high.Canine distemper virus in the thirty-eight samples had been tested by the TaqMan-based real-time RT-PCR,the common RT-PCR and electron microscopy.The sensitivity of the TaqMan-based real-time RT-PCR was higher than the common RT-PCR and electron microscopy.This suggests that the method may be used in clinical diagnosis,epizootic study and laboratory research.
文摘为建立一套最适合叶用莴苣(Lactuca sativa L.)基因表达量研究的荧光定量PCR技术体系,以叶用莴苣S24为试材,采用SYBR Green Ⅰ法,利用叶用莴苣内参基因的2对引物组合18SR1和18SR2,分别在10μL和20μL反应体系条件下进行扩增反应。结果表明,分别在10μL和20μL反应体系条件下内参基因引物组合18SR1的扩增曲线、标准曲线以及溶解曲线等指标都优于内参基因引物组合18SR2;2对内参基因引物组合18SR1和18SR2在10μL反应体系中的扩增曲线、标准曲线以及溶解曲线等指标也均优于20μL反应体系。因此叶用莴苣荧光定量PCR内参基因利用18SR1引物组合,反应体系采用10μL比较合理。
