Preliminary studies were done on the factors affecting the callus inducement from the anther of Jartopa curcas L.,including the development stage of microspores,cold pretreatment,hormone and sucrose concentration.The ...Preliminary studies were done on the factors affecting the callus inducement from the anther of Jartopa curcas L.,including the development stage of microspores,cold pretreatment,hormone and sucrose concentration.The results showed: compared with other stage,microspores at uninucleate stage were more suitable for culture;the cold pretreatment at 4℃ for 3~5 days is essential to high ratio of inducement;the culture medium that MS+ NAA 2.0mg/L +KT 0.4mg/L +sucrose 9% is comparativelymore suitable for callus inducement.展开更多
文摘Preliminary studies were done on the factors affecting the callus inducement from the anther of Jartopa curcas L.,including the development stage of microspores,cold pretreatment,hormone and sucrose concentration.The results showed: compared with other stage,microspores at uninucleate stage were more suitable for culture;the cold pretreatment at 4℃ for 3~5 days is essential to high ratio of inducement;the culture medium that MS+ NAA 2.0mg/L +KT 0.4mg/L +sucrose 9% is comparativelymore suitable for callus inducement.
基金supported by the National Natural Science Foundation of China(31170186,31110103911)the Main Direction Program of Knowledge Innovation of the Chinese Academy of Sciences(KSCX2-EW-Z-1)
基金supported by the National Natural Science Foundation of China(31170186,31370232)the Main Direction Program of Knowledge Innovation of Chinese Academy of Sciences(KSCX2-EW-Z-1)
基金supported by the National Natural Science Foundation of China(31170186,31110103911)the Main Direction Program of Knowledge Innovation of the Chinese Academy of Sciences(KSCX2-EW-Z-1)
文摘通过文献和标本考证,发现《中国植物志》第77卷第2分册和(Flora of China))20~21卷中均误将黑穗橐吾(Ligularia melanothyrsa Hand.-Mazz.)鉴定为植夫橐吾(L.fangiana Hand.-Mazz.),而真正的植夫橐吾应处理为川鄂橐吾[L.wilsoniana(Hemsl.)Greenm.]的异名。
基金supported by the National Natural Science Foundation of China(31170186,31110103911)the Main Direction Program of Knowledge Innovation of the Chinese Academy of Sciences(KSCX2-EW-Z-1)