It has long been found that insulin in acid solution is unstable. The main reason is that the A-chain C-terminal residue, asparagine, is very susceptible to acid hydrolysis, thereby a covalent-dimer formation takes pl...It has long been found that insulin in acid solution is unstable. The main reason is that the A-chain C-terminal residue, asparagine, is very susceptible to acid hydrolysis, thereby a covalent-dimer formation takes place readily due to the deamidation of this residue. As a number of pharmaceutical preparations of insulin are acidic solutions, this kind of instability has brought about a serious problem on purity and storage of this preparation in展开更多
Ⅰ. INTRODUCTIONImmune reaction remains a challenging problem in the clinical application of various insulin preparations. It is known that about 70％ of patients will produce anti-insulin antibodies after some time e...Ⅰ. INTRODUCTIONImmune reaction remains a challenging problem in the clinical application of various insulin preparations. It is known that about 70％ of patients will produce anti-insulin antibodies after some time even if highly pure insulin is used to treat diabetes. Therefore,it is both theoretically and practically significant to understand the molecular basis of insulin immune reaction and to find new insulin preparations with low antigenicity and full bioactivity.展开更多
1 Introduction The natural insulin in acidic solution is a short-acting preparation of which the diabetics need several injections per day to keep the necessary effect. Therefore, it has been a primary request to sear...1 Introduction The natural insulin in acidic solution is a short-acting preparation of which the diabetics need several injections per day to keep the necessary effect. Therefore, it has been a primary request to search for a long-acting insulin preparation from the beginning of medial me in practice. After the explorations for a long time, a series of prolonged-acting insulin preparations have been used in the treatment of diabetes. But most of them展开更多
基金Project supported by UNIDO(91/048)and High-Tech Program of China(103-19-01).
文摘It has long been found that insulin in acid solution is unstable. The main reason is that the A-chain C-terminal residue, asparagine, is very susceptible to acid hydrolysis, thereby a covalent-dimer formation takes place readily due to the deamidation of this residue. As a number of pharmaceutical preparations of insulin are acidic solutions, this kind of instability has brought about a serious problem on purity and storage of this preparation in
基金Supported by a grant of UNIDO (91/048/CW)and "863" high technology program
文摘Ⅰ. INTRODUCTIONImmune reaction remains a challenging problem in the clinical application of various insulin preparations. It is known that about 70％ of patients will produce anti-insulin antibodies after some time even if highly pure insulin is used to treat diabetes. Therefore,it is both theoretically and practically significant to understand the molecular basis of insulin immune reaction and to find new insulin preparations with low antigenicity and full bioactivity.
基金Project supported by grants of UNIDO (91/048) and "863" High-Fech Program.
文摘1 Introduction The natural insulin in acidic solution is a short-acting preparation of which the diabetics need several injections per day to keep the necessary effect. Therefore, it has been a primary request to search for a long-acting insulin preparation from the beginning of medial me in practice. After the explorations for a long time, a series of prolonged-acting insulin preparations have been used in the treatment of diabetes. But most of them
