WT5BZ]There are various arguments on classification of the genus Actinidia Lindl., a genus with approximately 63 species, 59 of which have been found in China. The paper investigated the characteristics of foliar tric...WT5BZ]There are various arguments on classification of the genus Actinidia Lindl., a genus with approximately 63 species, 59 of which have been found in China. The paper investigated the characteristics of foliar trichomes of 35 taxa from China under optical microscope, including size, celluar structure, distribution and density. According to their micromorphological characteristics, foliar trichomes can be classified into the following six categories: 1) single cell hairs; 2) uniseriate hairs, including linear, bulbous, twisted, straight walled, and bent walled hairs; 3) multiseriate hairs, including twisted, straight walled and gradually sharpening, straight walled and suddenly sharpening, bent walled and gradually sharpening, and suddenly sharpening hairs; 4) multiseriate thick hairs, including pillar hairs, gradually sharpening thick hairs, and suddenly sharpening thick hairs; 5) stellate hairs, including parenchyma stellate and sclerenchyma stellate (normal state and special states such as rosulate, peltate stellate, and overlopping stellate) hairs ; and 6) dichotomous hairs. On the basis of the micromorphological characteristics of foliar trichomes in Actinidia, with Clematoclethra lasioclada as an outgroup, both the quantitative cladistic analysis and phenetic analysis were performed using Wagner method and UPGMA clustering method respectively to reconstruct the phylogeny of Actinidia in China. The phylogenetic tree generated by cladistic analysis suggests that the sect. Leiocarpae be a monophyletic group, but other three sections, i.e., sect. Maculatae, sect. Strigosae and sect. Stellatae, be non monophyletic groups. The results obtained from the phenetic analysis reflect relationships among the taxa of Actinidia in China, especially a close relationship between A. chinensis and A. deliciosa, and a relatively remote relationship between A. callosa var. henryi and A. callosa var. discolor. In conclusion, the micromorphological characters of foliar trichomes and the methods of quantitative taxonomic analysis are of key importance to studies on phylogenetic and phenetic relationships of Actinidia.展开更多
A research of interphase nuclei and chromosome number of Isoetes sinensis Palmer was made in the article. The chromosome number of Isoetes sinensis Palmer was 2n=4x=44. The interphase nuclei were categorized to be sim...A research of interphase nuclei and chromosome number of Isoetes sinensis Palmer was made in the article. The chromosome number of Isoetes sinensis Palmer was 2n=4x=44. The interphase nuclei were categorized to be simple chromocenter type. The metaphase chromosomes were about 5 μm in length.In addition,the mitotic process was observed. These results could supply the cytological evidence for the exploration of the biosystematics and endangerment mechanism of this species.展开更多
取一雌株美味猕猴桃(Actinidia deliciosa line No.26)当年生枝条的茎段再生苗的叶片、幼嫩茎段及叶柄分别在培养基上诱导愈伤组织。上述3种不同愈伤组织来源的原生质体经培养后,叶片及茎段愈伤组织来源的原生质体再生大量小植株。在198...取一雌株美味猕猴桃(Actinidia deliciosa line No.26)当年生枝条的茎段再生苗的叶片、幼嫩茎段及叶柄分别在培养基上诱导愈伤组织。上述3种不同愈伤组织来源的原生质体经培养后,叶片及茎段愈伤组织来源的原生质体再生大量小植株。在1987—1989年间,再生小植株通过移栽或嫁接,有76株叶片愈伤组织及21株茎段愈伤组织来源的原生质体再生植株定植成活。叶片愈伤组织来源的原生质体再生植株中的3株,在1991年5月开花。其中2株是雌株,1株是雄株。经人工授粉后,雌株已结不少果实。从而证实,美味猕猴桃的体细胞再生植株存在性别分化。再生植株的叶片、节间及花的形态上出现大量的体细胞无性系变异。鉴定了16株再生植株染色体,其染色体数每株不一样,变动在116—180之间。展开更多
文摘WT5BZ]There are various arguments on classification of the genus Actinidia Lindl., a genus with approximately 63 species, 59 of which have been found in China. The paper investigated the characteristics of foliar trichomes of 35 taxa from China under optical microscope, including size, celluar structure, distribution and density. According to their micromorphological characteristics, foliar trichomes can be classified into the following six categories: 1) single cell hairs; 2) uniseriate hairs, including linear, bulbous, twisted, straight walled, and bent walled hairs; 3) multiseriate hairs, including twisted, straight walled and gradually sharpening, straight walled and suddenly sharpening, bent walled and gradually sharpening, and suddenly sharpening hairs; 4) multiseriate thick hairs, including pillar hairs, gradually sharpening thick hairs, and suddenly sharpening thick hairs; 5) stellate hairs, including parenchyma stellate and sclerenchyma stellate (normal state and special states such as rosulate, peltate stellate, and overlopping stellate) hairs ; and 6) dichotomous hairs. On the basis of the micromorphological characteristics of foliar trichomes in Actinidia, with Clematoclethra lasioclada as an outgroup, both the quantitative cladistic analysis and phenetic analysis were performed using Wagner method and UPGMA clustering method respectively to reconstruct the phylogeny of Actinidia in China. The phylogenetic tree generated by cladistic analysis suggests that the sect. Leiocarpae be a monophyletic group, but other three sections, i.e., sect. Maculatae, sect. Strigosae and sect. Stellatae, be non monophyletic groups. The results obtained from the phenetic analysis reflect relationships among the taxa of Actinidia in China, especially a close relationship between A. chinensis and A. deliciosa, and a relatively remote relationship between A. callosa var. henryi and A. callosa var. discolor. In conclusion, the micromorphological characters of foliar trichomes and the methods of quantitative taxonomic analysis are of key importance to studies on phylogenetic and phenetic relationships of Actinidia.
文摘A research of interphase nuclei and chromosome number of Isoetes sinensis Palmer was made in the article. The chromosome number of Isoetes sinensis Palmer was 2n=4x=44. The interphase nuclei were categorized to be simple chromocenter type. The metaphase chromosomes were about 5 μm in length.In addition,the mitotic process was observed. These results could supply the cytological evidence for the exploration of the biosystematics and endangerment mechanism of this species.
文摘取一雌株美味猕猴桃(Actinidia deliciosa line No.26)当年生枝条的茎段再生苗的叶片、幼嫩茎段及叶柄分别在培养基上诱导愈伤组织。上述3种不同愈伤组织来源的原生质体经培养后,叶片及茎段愈伤组织来源的原生质体再生大量小植株。在1987—1989年间,再生小植株通过移栽或嫁接,有76株叶片愈伤组织及21株茎段愈伤组织来源的原生质体再生植株定植成活。叶片愈伤组织来源的原生质体再生植株中的3株,在1991年5月开花。其中2株是雌株,1株是雄株。经人工授粉后,雌株已结不少果实。从而证实,美味猕猴桃的体细胞再生植株存在性别分化。再生植株的叶片、节间及花的形态上出现大量的体细胞无性系变异。鉴定了16株再生植株染色体,其染色体数每株不一样,变动在116—180之间。
