目的:观察浅针疗法对原发性失眠患者自主神经功能的影响。方法:将60例原发性失眠患者按照随机数字表法分为浅针组、艾灸组和经颅磁组,每组各20例。浅针组给予浅针镇静穴治疗,艾灸组给予艾灸镇静穴治疗,经颅磁组给予超低频重复经颅磁刺...目的:观察浅针疗法对原发性失眠患者自主神经功能的影响。方法:将60例原发性失眠患者按照随机数字表法分为浅针组、艾灸组和经颅磁组,每组各20例。浅针组给予浅针镇静穴治疗,艾灸组给予艾灸镇静穴治疗,经颅磁组给予超低频重复经颅磁刺激治疗,各组治疗均持续15min,并在治疗前、治疗中、治疗后15min分别对使用穿戴式心电贴对患者心率变异性进行记录,包括心率(beats per minutes, BPM)、心率变异性的时域指标[全部正常RR间期的标准差(Standard deviation of NN intervals for period of interest, SDNN)、相邻RR间期差值的均方根(Root mean square of successive differences of NN intervals for period of interest, rMSSD)]及心率变异性的频域指标[低频功率(low frequency, LF)、高频功率(high frequency power, HF)、低频功率与高频功率之比(LF/HF)]。结果:与本组治疗前比较,浅针组、艾灸组、经颅磁组治疗中、治疗后BPM均降低,差异具有统计学意义(P<0.05);3组患者治疗中、治疗后BPM组间比较,差异无统计学意义(P>0.05)。与本组治疗前比较,浅针组、艾灸组、经颅磁组治疗中、治疗后SDNN、rMSSD均升高,差异具有统计学意义(P<0.05);浅针组治疗后SDNN、rMSSD高于治疗中,经颅磁组治疗后SDNN高于治疗中,治疗后浅针组SDNN、rMSSD高于艾灸组及经颅磁组,差异均有统计学意义(P<0.05)。与治疗前比较,浅针组、经颅磁组治疗中、治疗后LF、LF/HF均降低,HF均升高,差异具有统计学意义(P<0.05);治疗后艾灸组LF低于治疗中,差异具有统计学意义(P<0.05);治疗后浅针组HF高于经颅磁组,LF/HF低于艾灸组、经颅磁组,且艾灸组治疗后LF/HF经颅磁组,差异具有统计学意义(P<0.05)。结论:浅针疗法可改善原发性失眠患者交感神经系统和副交感神经系统之间的整体平衡,从而改善自主神经功能。展开更多
目的观察针刺肩痛穴联合伤药软膏外敷、功法锻炼治疗肩关节周围炎(简称肩周炎)的临床疗效。方法将60例肩周炎患者按照随机数字表法分为试验组和对照组,各30例。对照组进行伤药软膏外敷、功法锻练;试验组在对照组的治疗基础上加针刺肩痛...目的观察针刺肩痛穴联合伤药软膏外敷、功法锻炼治疗肩关节周围炎(简称肩周炎)的临床疗效。方法将60例肩周炎患者按照随机数字表法分为试验组和对照组,各30例。对照组进行伤药软膏外敷、功法锻练;试验组在对照组的治疗基础上加针刺肩痛穴。治疗2周后,比较2组视觉疼痛模拟量表(Visual analogue scale,VAS)评分、关节活动度(Range of motion,ROM)及总体疗效评定。结果2组治疗后的VAS评分较治疗前低,且试验组VAS评分较对照组低,差异均有统计学意义(P<0.05);2组治疗后的ROM较治疗前改善,差异有统计学意义(P<0.05);2组治疗总有效率比较,差异无统计学意义(P>0.05)。结论伤药软膏外敷、功法锻练与伤药软膏外敷、功法锻练基础上加针刺肩痛穴的疗效相当,均能改善ROM,但伤药软膏外敷、功法锻练基础上加针刺肩痛穴能减轻患者的疼痛,值得临床应用推广。展开更多
Objective： To evaluate the effect of Zhuanggu Jianxi Decoction （壮骨健膝方, ZGJXD） on interleukin- 1 β- （IL-1 β- ）-induced degeneration of chondrocytes （CDs） as well as the activation of caveolin-p38 mitogen-...Objective： To evaluate the effect of Zhuanggu Jianxi Decoction （壮骨健膝方, ZGJXD） on interleukin- 1 β- （IL-1 β- ）-induced degeneration of chondrocytes （CDs） as well as the activation of caveolin-p38 mitogen- activated protein kinase （MAPK） signal pathway, investigating the possible molecular mechanism that ZGJXD treats osteoarthritis. Methods： Serum pharmacology was applied in the present study, where ZGJXD was orally administrated to New Zealand rabbits and then ZGJXD containing serum （ZGJXD-S） was collected for following in vitro experiments. CDs were isolated aseptically from New Zealand rabbits and then cultured in vitro. Upon IL- l β-stimulation, the degeneration of CDs was verified by inverted microscope, toluidine blue stain and type H collagen immunocytochemistry. After IL-1 β-stimulated CDs were intervened with blank control serum, ZGJXD-S, together with or without SB203580 （a specific inhibitor of p38 MAPK） for 48 h, caveoUn-1 protein expression and the phosphorylation level of p38 were determined by Western blotting, and the mRNA expression of IL- 1β-, tumor necrosis factor ~ （TNF-oL）, matrix metalloproteinase 3 （MMP-3） and MMP-β were examined by real-time polymerase chain reaction. Results： IL-1 β- stimulation induced degeneration of CDs, increased caveolin-1 expression and p38 phosphorylation, up-regulated the mRNA level of IL-1 β, TNF-β, MMP-3 and MMP-β. However, the IL-1 β-induced activation of caveolin-p38 signaling and alteration in the expression of p38 downstream target genes were suppressed by ZGJXD-S and/or SB203580 in CDs. Conclusion： ZGJXD can prevent CDs degeneration via inhibition of caveolin-p38 MAPK signal pathway, which might be one of the mechanisms that ZGJXD treats osteoarthritis.展开更多
文摘目的:观察浅针疗法对原发性失眠患者自主神经功能的影响。方法:将60例原发性失眠患者按照随机数字表法分为浅针组、艾灸组和经颅磁组,每组各20例。浅针组给予浅针镇静穴治疗,艾灸组给予艾灸镇静穴治疗,经颅磁组给予超低频重复经颅磁刺激治疗,各组治疗均持续15min,并在治疗前、治疗中、治疗后15min分别对使用穿戴式心电贴对患者心率变异性进行记录,包括心率(beats per minutes, BPM)、心率变异性的时域指标[全部正常RR间期的标准差(Standard deviation of NN intervals for period of interest, SDNN)、相邻RR间期差值的均方根(Root mean square of successive differences of NN intervals for period of interest, rMSSD)]及心率变异性的频域指标[低频功率(low frequency, LF)、高频功率(high frequency power, HF)、低频功率与高频功率之比(LF/HF)]。结果:与本组治疗前比较,浅针组、艾灸组、经颅磁组治疗中、治疗后BPM均降低,差异具有统计学意义(P<0.05);3组患者治疗中、治疗后BPM组间比较,差异无统计学意义(P>0.05)。与本组治疗前比较,浅针组、艾灸组、经颅磁组治疗中、治疗后SDNN、rMSSD均升高,差异具有统计学意义(P<0.05);浅针组治疗后SDNN、rMSSD高于治疗中,经颅磁组治疗后SDNN高于治疗中,治疗后浅针组SDNN、rMSSD高于艾灸组及经颅磁组,差异均有统计学意义(P<0.05)。与治疗前比较,浅针组、经颅磁组治疗中、治疗后LF、LF/HF均降低,HF均升高,差异具有统计学意义(P<0.05);治疗后艾灸组LF低于治疗中,差异具有统计学意义(P<0.05);治疗后浅针组HF高于经颅磁组,LF/HF低于艾灸组、经颅磁组,且艾灸组治疗后LF/HF经颅磁组,差异具有统计学意义(P<0.05)。结论:浅针疗法可改善原发性失眠患者交感神经系统和副交感神经系统之间的整体平衡,从而改善自主神经功能。
文摘目的观察针刺肩痛穴联合伤药软膏外敷、功法锻炼治疗肩关节周围炎(简称肩周炎)的临床疗效。方法将60例肩周炎患者按照随机数字表法分为试验组和对照组,各30例。对照组进行伤药软膏外敷、功法锻练;试验组在对照组的治疗基础上加针刺肩痛穴。治疗2周后,比较2组视觉疼痛模拟量表(Visual analogue scale,VAS)评分、关节活动度(Range of motion,ROM)及总体疗效评定。结果2组治疗后的VAS评分较治疗前低,且试验组VAS评分较对照组低,差异均有统计学意义(P<0.05);2组治疗后的ROM较治疗前改善,差异有统计学意义(P<0.05);2组治疗总有效率比较,差异无统计学意义(P>0.05)。结论伤药软膏外敷、功法锻练与伤药软膏外敷、功法锻练基础上加针刺肩痛穴的疗效相当,均能改善ROM,但伤药软膏外敷、功法锻练基础上加针刺肩痛穴能减轻患者的疼痛,值得临床应用推广。
基金Supported by the National Natural Science Foundation of China(No.81072828)
文摘Objective： To evaluate the effect of Zhuanggu Jianxi Decoction （壮骨健膝方, ZGJXD） on interleukin- 1 β- （IL-1 β- ）-induced degeneration of chondrocytes （CDs） as well as the activation of caveolin-p38 mitogen- activated protein kinase （MAPK） signal pathway, investigating the possible molecular mechanism that ZGJXD treats osteoarthritis. Methods： Serum pharmacology was applied in the present study, where ZGJXD was orally administrated to New Zealand rabbits and then ZGJXD containing serum （ZGJXD-S） was collected for following in vitro experiments. CDs were isolated aseptically from New Zealand rabbits and then cultured in vitro. Upon IL- l β-stimulation, the degeneration of CDs was verified by inverted microscope, toluidine blue stain and type H collagen immunocytochemistry. After IL-1 β-stimulated CDs were intervened with blank control serum, ZGJXD-S, together with or without SB203580 （a specific inhibitor of p38 MAPK） for 48 h, caveoUn-1 protein expression and the phosphorylation level of p38 were determined by Western blotting, and the mRNA expression of IL- 1β-, tumor necrosis factor ~ （TNF-oL）, matrix metalloproteinase 3 （MMP-3） and MMP-β were examined by real-time polymerase chain reaction. Results： IL-1 β- stimulation induced degeneration of CDs, increased caveolin-1 expression and p38 phosphorylation, up-regulated the mRNA level of IL-1 β, TNF-β, MMP-3 and MMP-β. However, the IL-1 β-induced activation of caveolin-p38 signaling and alteration in the expression of p38 downstream target genes were suppressed by ZGJXD-S and/or SB203580 in CDs. Conclusion： ZGJXD can prevent CDs degeneration via inhibition of caveolin-p38 MAPK signal pathway, which might be one of the mechanisms that ZGJXD treats osteoarthritis.
