Betaine was accumulated as a nontoxic and protective osmolyte in water-dificit and salt-stressed plants.Be-taine aldehyde dehydrogenase for glycine betaine synthesis is an important enzyme.The BADH gene of Maizewas cl...Betaine was accumulated as a nontoxic and protective osmolyte in water-dificit and salt-stressed plants.Be-taine aldehyde dehydrogenase for glycine betaine synthesis is an important enzyme.The BADH gene of Maizewas cloned by RT-PCR and RACE.The gene is 1 762 bp in length,including an open reading frame of 1 515 bp.Its nucleotide sequence shares 95% with the the partial cDNA of BADH1 from Sorghum bicolor.Its deducedamino acid sequence contains the conserved domain sequence 'VTLELGGKSP' of ALDH,and a tripeptide SKLat its C-terminal,a signal targetting to the microbodies.The phylogenetic tree of 20 BADHs was constructed,which corresponds to the classical botanical division of plant,the BADH of maize is closest to the one of Oryza Sativa.GenBank accession No.AY587278.展开更多
进行了甘蔗属近缘野生种——斑茅抗逆性基因克隆的研究。以斑茅为材料,用RACE技术克隆了斑茅的20S蛋白酶体α亚基5基因,其编码区为711 bp,编码237个氨基酸,并于genbank分析其核苷酸和推导的氨基酸序列的同源性,用Clustalx version 1.8...进行了甘蔗属近缘野生种——斑茅抗逆性基因克隆的研究。以斑茅为材料,用RACE技术克隆了斑茅的20S蛋白酶体α亚基5基因,其编码区为711 bp,编码237个氨基酸,并于genbank分析其核苷酸和推导的氨基酸序列的同源性,用Clustalx version 1.8软件分析其系统进化关系。结果表明,物种间蛋白酶体进化保守,其核苷酸序列与水稻、大豆、剑叶藓属植物的相应基因同源性分别为91％、81％、80％;其氨基酸序列与水稻、大豆、剑叶藓属植物的同源性分别达97％、95％、86％,与人的同源性也高达69％。展开更多
基金supported by the National Foundation Natural Science of China(No.30170590).
文摘Betaine was accumulated as a nontoxic and protective osmolyte in water-dificit and salt-stressed plants.Be-taine aldehyde dehydrogenase for glycine betaine synthesis is an important enzyme.The BADH gene of Maizewas cloned by RT-PCR and RACE.The gene is 1 762 bp in length,including an open reading frame of 1 515 bp.Its nucleotide sequence shares 95% with the the partial cDNA of BADH1 from Sorghum bicolor.Its deducedamino acid sequence contains the conserved domain sequence 'VTLELGGKSP' of ALDH,and a tripeptide SKLat its C-terminal,a signal targetting to the microbodies.The phylogenetic tree of 20 BADHs was constructed,which corresponds to the classical botanical division of plant,the BADH of maize is closest to the one of Oryza Sativa.GenBank accession No.AY587278.
文摘进行了甘蔗属近缘野生种——斑茅抗逆性基因克隆的研究。以斑茅为材料,用RACE技术克隆了斑茅的20S蛋白酶体α亚基5基因,其编码区为711 bp,编码237个氨基酸,并于genbank分析其核苷酸和推导的氨基酸序列的同源性,用Clustalx version 1.8软件分析其系统进化关系。结果表明,物种间蛋白酶体进化保守,其核苷酸序列与水稻、大豆、剑叶藓属植物的相应基因同源性分别为91％、81％、80％;其氨基酸序列与水稻、大豆、剑叶藓属植物的同源性分别达97％、95％、86％,与人的同源性也高达69％。
文摘大多数信号转导过程都伴随着细胞内钙离子浓度变化,CBL/CIPK信号系统是近几年在高等植物中发现的一类依赖于钙离子的信号系统,包括感知钙离子浓度变化的CBL蛋白(calcineurin B-like proteins)和与之互作的CIPK蛋白(CBL-interacting protein kinase)。研究表明CBL/CIPK信号系统在植物对逆境应答过程中起重要作用。在介绍CBL和CIPK的结构和特征基础上对不同植物CBL/CIPK信号系统在逆境应答中的研究现状况进行了综述,以期为基因工程改良作物抗逆育种提供新的思路和种质资源。
