目的观察蛋白酪氨酸磷酸酶1B(PTP1B)及Sam68(Src-associated substrated during mitosis of 68KD)在结直肠癌的发生发展中的表达及其相关性,探究其与结直肠癌临床病理特征的关系。方法采用免疫组织化学(SABC)法检测53例结直癌组织及20...目的观察蛋白酪氨酸磷酸酶1B(PTP1B)及Sam68(Src-associated substrated during mitosis of 68KD)在结直肠癌的发生发展中的表达及其相关性,探究其与结直肠癌临床病理特征的关系。方法采用免疫组织化学(SABC)法检测53例结直癌组织及20例癌旁正常组织中PTP1B、Sam68的表达情况,并结合临床病理学资料进行统计分析;采用Western blotting方法检测12例结直肠癌组织及对应癌旁正常组织中的PTP1B及Sam68蛋白表达情况。结果免疫组化结果显示PTP1B、Sam68在结直肠癌组织的阳性表达率明显高于正常大肠组织,差异均有统计学意义(均P<0.001)。PTP1B、Sam68的表达均与分化程度、TNM分期及淋巴结转移相关(P<0.05);相关性分析显示,PTP1B与Sam68表达呈正相关(r=0.463,P<0.001)。Western blotting检测结果显示结直肠癌组织中PTP1B和Sam68的蛋白表达量明显高于癌旁正常组织(P<0.05)。结论 PTP1B、Sam68可能与大肠癌的发生、浸润及转移有关,两者共同促进大肠癌的发生、发展、侵袭及转移。展开更多
This study aimed to investigate the expression of β-catenin in hepatocellular carcinoma（HCC） tissues and its relationship with α-fetoprotein（AFP） in HCC.Immunohistochemistry was used to determine the expression ...This study aimed to investigate the expression of β-catenin in hepatocellular carcinoma（HCC） tissues and its relationship with α-fetoprotein（AFP） in HCC.Immunohistochemistry was used to determine the expression of β-catenin in normal liver tissues（n=10）,liver cirrhosis tissues（n=20）,and primary HCC tissues（n=60）.The relationship between β-catenin expression and clinical parameters of HCC was investigated.Real-time PCR and Western blotting were used to detect the m RNA and protein expression levels of β-catenin in the liver cancer cell line SMMC-7721 transfected with a plasmid encoding AFP,and also the m RNA and protein expression levels of β-catenin were measured in the liver cancer cell line Huh7 before and after the transfection with AFP sh RNA plasmids.The results showed that β-catenin was only expressed on the cell membrane in normal liver tissues.Its localization to the cytoplasm and nucleus of cells was observed in a small proportion of cirrhotic tissues or adjacent HCC tissues,and such ectopic expression of β-catenin was predominant in HCC tissues.The abnormal expression of β-catenin was correlated with serum AFP levels,cancer cell differentiation and vascular invasion（P〈0.05）.Additionally,the increased expression of AFP resulted in the upregulation of β-catenin m RNA and protein levels,while knockdown of AFP with AFP sh RNA led to significantly decreased β-catenin m RNA and protein levels（P〈0.05）.It was suggested that the abnormal expression of β-catenin is implicated in hepatic carcinogenesis and development.AFP can lead to increased expression of β-catenin,which may account for the poor prognosis of AFP-associated HCC patients.展开更多
文摘目的观察蛋白酪氨酸磷酸酶1B(PTP1B)及Sam68(Src-associated substrated during mitosis of 68KD)在结直肠癌的发生发展中的表达及其相关性,探究其与结直肠癌临床病理特征的关系。方法采用免疫组织化学(SABC)法检测53例结直癌组织及20例癌旁正常组织中PTP1B、Sam68的表达情况,并结合临床病理学资料进行统计分析;采用Western blotting方法检测12例结直肠癌组织及对应癌旁正常组织中的PTP1B及Sam68蛋白表达情况。结果免疫组化结果显示PTP1B、Sam68在结直肠癌组织的阳性表达率明显高于正常大肠组织,差异均有统计学意义(均P<0.001)。PTP1B、Sam68的表达均与分化程度、TNM分期及淋巴结转移相关(P<0.05);相关性分析显示,PTP1B与Sam68表达呈正相关(r=0.463,P<0.001)。Western blotting检测结果显示结直肠癌组织中PTP1B和Sam68的蛋白表达量明显高于癌旁正常组织(P<0.05)。结论 PTP1B、Sam68可能与大肠癌的发生、浸润及转移有关,两者共同促进大肠癌的发生、发展、侵袭及转移。
文摘This study aimed to investigate the expression of β-catenin in hepatocellular carcinoma（HCC） tissues and its relationship with α-fetoprotein（AFP） in HCC.Immunohistochemistry was used to determine the expression of β-catenin in normal liver tissues（n=10）,liver cirrhosis tissues（n=20）,and primary HCC tissues（n=60）.The relationship between β-catenin expression and clinical parameters of HCC was investigated.Real-time PCR and Western blotting were used to detect the m RNA and protein expression levels of β-catenin in the liver cancer cell line SMMC-7721 transfected with a plasmid encoding AFP,and also the m RNA and protein expression levels of β-catenin were measured in the liver cancer cell line Huh7 before and after the transfection with AFP sh RNA plasmids.The results showed that β-catenin was only expressed on the cell membrane in normal liver tissues.Its localization to the cytoplasm and nucleus of cells was observed in a small proportion of cirrhotic tissues or adjacent HCC tissues,and such ectopic expression of β-catenin was predominant in HCC tissues.The abnormal expression of β-catenin was correlated with serum AFP levels,cancer cell differentiation and vascular invasion（P〈0.05）.Additionally,the increased expression of AFP resulted in the upregulation of β-catenin m RNA and protein levels,while knockdown of AFP with AFP sh RNA led to significantly decreased β-catenin m RNA and protein levels（P〈0.05）.It was suggested that the abnormal expression of β-catenin is implicated in hepatic carcinogenesis and development.AFP can lead to increased expression of β-catenin,which may account for the poor prognosis of AFP-associated HCC patients.
