Background Hyperuricemia （HUA） and hypertension are associated with the increasing risk and mortality of cardiovascular disease （CVD）. However, the relationship between body mass index （BMI） and HUA in hypertens...Background Hyperuricemia （HUA） and hypertension are associated with the increasing risk and mortality of cardiovascular disease （CVD）. However, the relationship between body mass index （BMI） and HUA in hypertensive adults over 80 years remains uncertain. Methods Observational trial was designed and 308 patients who were newly diagnosed as essential hypertension without anti-hypertensive therapy were enrolled into our study. According to the cut-off value of serum UA, participants were stratified into normal （ 〈420μmol/L for men and 〈360 μmol/L for women） and increased UA groups （≥420 μmol/L for men and ≥360 μmol/L for women）. Serum UA level, blood pressure and other baseline characteristics were compared, logistic regression analysis and receiver operating characteristic curve （ROC） were performed. Results The mean（SD） serum uric level was 382.2 （108.7） μmol/L and the prevalence of hyperuricemia was 45% among men and 50% among women. BMI was significantly higher （22.6 vs. 24.0 kg/m^2, P=0.003） and FBG was lower （5.13 vs. 4.98 mmol/L, P=0.025） in increased UA group among aged women, and BMI and FBG were found the independent determinants for UA increase in female subjects according to logistic regression analysis, and the odd ratio were 1.154（95% interval confidence 1.058- 1.259, P=0.001） and 0.646 （95% interval confidence 0.44-0.949, P=0.026） , respectively. Moreover, evaluation of receiver operating characteristic curve （ROC）showed that area under the curve for BMI to predict UA increase was 0.627±0.039, P=0.001 in women. However, the results mentioned above were only found in elderly women, not in men. Conclusions Our study indicates that aged women have higher prevalence of HUA than men, and that BMI is independently associated with serum uric acid level for hypertensive women but not for men over 80 years old. Therefore, BMI is a useful predictor of serum uric acid level in el- derly women with hypertension.展开更多
Background Atherosclerosis is a major health problem worldwide. Previous studies have shown that miR- NAs are related to atherosclerosis. However, the relationship between miR-21 and atherosclerosis remains un- known....Background Atherosclerosis is a major health problem worldwide. Previous studies have shown that miR- NAs are related to atherosclerosis. However, the relationship between miR-21 and atherosclerosis remains un- known. Therefore, this study aimed to explore the relationship between plasma miR-21 expression level and subclinical atherosclerosis in patients with hypertension. Methods We assessed the plasma level of miR-21 in 50 hypertension patients and in 30 age- and sexmatched healthy individuals. All patients underwent brachial-ankle pulse wave velocity （baPWV）, office and ambulatory blood pressure monitoring. Quantitative reverse transcrip- tase polymerase chain reaction （qRT-PCR） was used to measure miR-21. The correlations between miR-21 level and blood pressure parameters and baPWV were assessed using the Pearson correlation coefficient. Receiver op- erating characteristic （ROC） curve was constructed for distinguishing from atherosclerosis and healthy. Results The baseline data of all the participants such as fasting blood glucose, body mass index, serum lipid, hepatic and renal function were not significantly different between hypertension and control groups, as well as atherosclerosis and non-atherosclerosis groups （P〉0.05）. The hypertensive patients showed higher plasma miR-21 （32.99±3.01 vs. 26.14±1.03; P〈0.001） level as compared to controls, and in atherosclerosis group （33.53±2.86 vs. 28.56±3.66; P=0.002） than that in non-atherosclerosis group. MiR-21 level was significant positively correlated with 24h mean SBP （r=0.718, P〈0.001）, 24 h mean DBP （r=0.247 P=0.027）, office SBP （r=0.644, P〈0.001）, office DBP （r=0.316, P=0.004） and baPWV （r=0.714, P〈0.001）, respectively. The miR-21 for distinguishing atherosclerosis from healthy yielded an area under the ROC curve of 0.844 （95%CI： 0.758, 0.930; P〈0.001）. Conclusion Our data provide significant evidence that circulating miR-21 may represent potential non-invasive marker of athero-sclerosis in essential hypertensive patients.展开更多
Background MicroRNA （miR）-92a has been demonstrated to be associated with atherosclerosis through different ways. However, there are limited date about the association of miR-92a with arteriosclerosis in hypertensiv...Background MicroRNA （miR）-92a has been demonstrated to be associated with atherosclerosis through different ways. However, there are limited date about the association of miR-92a with arteriosclerosis in hypertensive patients. Methods We assessed the plasma level of miR-92a in 110 patients with untreated essential hypertension and 65 healthy individuals. All patients underwent the measurement of brachial-ankle pulse wave velocity （baPWV） and ambulatory blood pressure. Quantitative reverse transcriptase polymerase chain reaction （qRT- PCR） was used to evaluate level of miR-92a. The correlations of miR-92a with blood pressure parameters and baPWV were assessed using the Spearman correlation coefficient. Receiver operating characteristic （ROC） curve was constructed and the area under the ROC curve （AUC） was generated to assess the diagnostic value of miR- 92a for arteriosclerosis. Results The plasma level of miR-92a was significantly higher in hypertensive patients than in control individuals（31.03±3.13 vs. 25.00±2.99; P 〈0.001）,and higher in arteriosclerosis group than in unarteriosclerosis group（33.74±2.43 vs. 27.51±3.28; P〈0.001）. There were significantly positive correlations between MiR-92a level with 24 h mean SBP（r=0.874, P〈0.001）, 24h mean DBP（r=0.680, P〈0.001） and baPWV（r=0.685, P〈0.001）. ROC curve analyses revealed that the AUC value for miR-92a was 0.929 （95%CI： 0.886, 0.973; P〈 0.001）. Conclusions The level of miR-92a is positively related to baPWV, and holds great potential to be a noninvasive screening marker for arteriosclerosis in essential hypertensive patients.展开更多
Background Studies have shown that mi RNA-155 played an important role in the process of development of hypertension.However,there is no date about mi RNA-155 and blood pressure monitoring parameters.Therefore,we exam...Background Studies have shown that mi RNA-155 played an important role in the process of development of hypertension.However,there is no date about mi RNA-155 and blood pressure monitoring parameters.Therefore,we examined whether in hypertensive patients the expression level of plasma mi RNA-155 related to 24-h ambulatory blood pressure monitoring（ABPM） parameters.Methods A cohort of adult patients scheduled to receive physical examination,office and ambulatory blood pressure monitoring.Quantitative reverse transcriptase polymerase chain reaction（q RT-PCR） was used to evaluate the expression of selected mi RNA-155.The mi RNA-155 expression level correlation between blood pressure parameters was assessed using the Spearman correlation coefficient.Results Fifty four essential hypertension patients（25 men;mean age,53.28 ± 9.52 years） and thirty healthy volunteers（15 men;mean age,53.03 ± 5.87 years） were included.We observed higher expression level of mi RNA-155（32.31 ± 2.85 vs 27.21 ± 1.59,P 〈 0.001） in hypertensive patients compared to healthy control individuals.MiRNA- 155 expression level showed significant positive correlation with 24 h Daytime SBP（r =0.681,P 〈 0.001）,24 h Daytime DBP（r = 0.473,P 〈 0.001）,24 h Daytime PP（r = 0.565,P 〈 0.001） and dipping（r = 0.257,P = 0.018）,respectively.Conclusions Our study showed that mi RNA-155 expression level was associated positively with daytime blood pressure monitoring parameters,as well as blood pressure variability,indicating a possible implication of miRNA- 155 in the pathogenesis of hypertension.展开更多
Background Micro RNAs have recently been considered as biomarkers in several different cardiovascular diseases,however,so far there are no circulating miRNAs data about hypertension.Therefore,the aim of the present pi...Background Micro RNAs have recently been considered as biomarkers in several different cardiovascular diseases,however,so far there are no circulating miRNAs data about hypertension.Therefore,the aim of the present pilot study was to identify circulating miRNAs for hypertension biomarkers.Methods Using an Agilent microarray,plasma miRNAs were profiled from plasma samples of 10 patients with untreated essential hypertension and 10 healthy controls.Candidate biomarkers identified in the profiles were subjected to validation by using quantitative PCR in an independent sample set of 20 patients with untreated essential hypertension and 20 healthy controls.Then,we assessed the selected miRNAs for the detection and diagnosis of hypertension from plasma samples of 70 patients with untreated essential hypertension and 20 healthy controls.The Spearman correlation coefficient was used to assessed the selected miRNAs correlations with blood pressure.The area under the receiver operating characteristic curve（AUC） was used to evaluate diagnostic accuracy.Results The expressions of selected 8 miRNAs were investigated independently in plasma samples from 10 hypertension patients and 10 healthy subjects.The levels of circulating miR-30c-5p,miR-133 b,miR-29b-3p,miR-29a-3p,miR-29c-3p,miR-30a-3p,miR-let7b-3p expression were significantly down regulated in hypertension group compared with healthy group and the level of hsa-miR-92b-3p was significantly unregulated between the groups.We used q RT-PCR assay to confirm the expression of 8 candidate miRNAs,miR-30c-5p（P 〈 0.001）,miR-29b-3p（P 〈 0.001）,miR-29a-3p（P = 0.027）,miR-29c-3p（P 〈 0.001）,miR-92b-3p（P = 0.003）,miR-30a-3p（P = 0.704）,miR-133b（P =0.346）,andmiR-let7b（P = 0.161）.The diagnostic accuracy of miR-30c-5p,miR-29b-3p,miR-29a-3p,miR-29c-3p and miR-92b-3p,as measured by AUC,were 0.897,0.90,0.829,0.825 and 0.832,respectively,with all P 〈0.001.Conclusions The plasma levels of miR-30c-5p,miR-29b-3p,miR-29a-3p,miR-29c-3p and miR-92b-3p associated with hypertension which provide an impetus for future evaluations of hypertension development,progression,therapeutic efficacy and prognosis.展开更多
基金supported by the grants from Guangdong Natural Science Foundation(No.2015A030313660)the Technology Project Foundation of Guangzhou(No.2014y2-00140,No.1563000381)the Technology Project Foundation of Guangdong Province(No.2014B020212008)
文摘Background Hyperuricemia （HUA） and hypertension are associated with the increasing risk and mortality of cardiovascular disease （CVD）. However, the relationship between body mass index （BMI） and HUA in hypertensive adults over 80 years remains uncertain. Methods Observational trial was designed and 308 patients who were newly diagnosed as essential hypertension without anti-hypertensive therapy were enrolled into our study. According to the cut-off value of serum UA, participants were stratified into normal （ 〈420μmol/L for men and 〈360 μmol/L for women） and increased UA groups （≥420 μmol/L for men and ≥360 μmol/L for women）. Serum UA level, blood pressure and other baseline characteristics were compared, logistic regression analysis and receiver operating characteristic curve （ROC） were performed. Results The mean（SD） serum uric level was 382.2 （108.7） μmol/L and the prevalence of hyperuricemia was 45% among men and 50% among women. BMI was significantly higher （22.6 vs. 24.0 kg/m^2, P=0.003） and FBG was lower （5.13 vs. 4.98 mmol/L, P=0.025） in increased UA group among aged women, and BMI and FBG were found the independent determinants for UA increase in female subjects according to logistic regression analysis, and the odd ratio were 1.154（95% interval confidence 1.058- 1.259, P=0.001） and 0.646 （95% interval confidence 0.44-0.949, P=0.026） , respectively. Moreover, evaluation of receiver operating characteristic curve （ROC）showed that area under the curve for BMI to predict UA increase was 0.627±0.039, P=0.001 in women. However, the results mentioned above were only found in elderly women, not in men. Conclusions Our study indicates that aged women have higher prevalence of HUA than men, and that BMI is independently associated with serum uric acid level for hypertensive women but not for men over 80 years old. Therefore, BMI is a useful predictor of serum uric acid level in el- derly women with hypertension.
基金supported by the grants from Guangdong Natural Science Foundation(No.S2013010016575/No.2015A030313660)the Technology Project Foundation of Guangzhou(No.2014y2-00140/No.1563000381)+1 种基金the Technology Project Foundation of Guangdong Province(No.2014B020212008)National Natural Science Foundation of China(No.81300230)
文摘Background Atherosclerosis is a major health problem worldwide. Previous studies have shown that miR- NAs are related to atherosclerosis. However, the relationship between miR-21 and atherosclerosis remains un- known. Therefore, this study aimed to explore the relationship between plasma miR-21 expression level and subclinical atherosclerosis in patients with hypertension. Methods We assessed the plasma level of miR-21 in 50 hypertension patients and in 30 age- and sexmatched healthy individuals. All patients underwent brachial-ankle pulse wave velocity （baPWV）, office and ambulatory blood pressure monitoring. Quantitative reverse transcrip- tase polymerase chain reaction （qRT-PCR） was used to measure miR-21. The correlations between miR-21 level and blood pressure parameters and baPWV were assessed using the Pearson correlation coefficient. Receiver op- erating characteristic （ROC） curve was constructed for distinguishing from atherosclerosis and healthy. Results The baseline data of all the participants such as fasting blood glucose, body mass index, serum lipid, hepatic and renal function were not significantly different between hypertension and control groups, as well as atherosclerosis and non-atherosclerosis groups （P〉0.05）. The hypertensive patients showed higher plasma miR-21 （32.99±3.01 vs. 26.14±1.03; P〈0.001） level as compared to controls, and in atherosclerosis group （33.53±2.86 vs. 28.56±3.66; P=0.002） than that in non-atherosclerosis group. MiR-21 level was significant positively correlated with 24h mean SBP （r=0.718, P〈0.001）, 24 h mean DBP （r=0.247 P=0.027）, office SBP （r=0.644, P〈0.001）, office DBP （r=0.316, P=0.004） and baPWV （r=0.714, P〈0.001）, respectively. The miR-21 for distinguishing atherosclerosis from healthy yielded an area under the ROC curve of 0.844 （95%CI： 0.758, 0.930; P〈0.001）. Conclusion Our data provide significant evidence that circulating miR-21 may represent potential non-invasive marker of athero-sclerosis in essential hypertensive patients.
基金supported by the grants from Guangdong Natural Science Foundation(No.2015A030313660)the Technology Project Foundation of Guangzhou(No.2014y2-00140/No.1563000381)the Technology Project Foundation of Guangdong Province(No.2014B020212008/No.2007B031500010)
文摘Background MicroRNA （miR）-92a has been demonstrated to be associated with atherosclerosis through different ways. However, there are limited date about the association of miR-92a with arteriosclerosis in hypertensive patients. Methods We assessed the plasma level of miR-92a in 110 patients with untreated essential hypertension and 65 healthy individuals. All patients underwent the measurement of brachial-ankle pulse wave velocity （baPWV） and ambulatory blood pressure. Quantitative reverse transcriptase polymerase chain reaction （qRT- PCR） was used to evaluate level of miR-92a. The correlations of miR-92a with blood pressure parameters and baPWV were assessed using the Spearman correlation coefficient. Receiver operating characteristic （ROC） curve was constructed and the area under the ROC curve （AUC） was generated to assess the diagnostic value of miR- 92a for arteriosclerosis. Results The plasma level of miR-92a was significantly higher in hypertensive patients than in control individuals（31.03±3.13 vs. 25.00±2.99; P 〈0.001）,and higher in arteriosclerosis group than in unarteriosclerosis group（33.74±2.43 vs. 27.51±3.28; P〈0.001）. There were significantly positive correlations between MiR-92a level with 24 h mean SBP（r=0.874, P〈0.001）, 24h mean DBP（r=0.680, P〈0.001） and baPWV（r=0.685, P〈0.001）. ROC curve analyses revealed that the AUC value for miR-92a was 0.929 （95%CI： 0.886, 0.973; P〈 0.001）. Conclusions The level of miR-92a is positively related to baPWV, and holds great potential to be a noninvasive screening marker for arteriosclerosis in essential hypertensive patients.
基金supported by the grants from the Natural Science Foundation of Guangdong Province(No.S2013010016575/No.2015A030313660)the Technology Project Foundation of Guangzhou(No.2014y2-00140/No.1563000381)+1 种基金the Technology Project Foundation of Guangdong Province(No.2014B020212008)the Natural Science Foundation of Guangdong Province(No.S2013010016575)
文摘Background Studies have shown that mi RNA-155 played an important role in the process of development of hypertension.However,there is no date about mi RNA-155 and blood pressure monitoring parameters.Therefore,we examined whether in hypertensive patients the expression level of plasma mi RNA-155 related to 24-h ambulatory blood pressure monitoring（ABPM） parameters.Methods A cohort of adult patients scheduled to receive physical examination,office and ambulatory blood pressure monitoring.Quantitative reverse transcriptase polymerase chain reaction（q RT-PCR） was used to evaluate the expression of selected mi RNA-155.The mi RNA-155 expression level correlation between blood pressure parameters was assessed using the Spearman correlation coefficient.Results Fifty four essential hypertension patients（25 men;mean age,53.28 ± 9.52 years） and thirty healthy volunteers（15 men;mean age,53.03 ± 5.87 years） were included.We observed higher expression level of mi RNA-155（32.31 ± 2.85 vs 27.21 ± 1.59,P 〈 0.001） in hypertensive patients compared to healthy control individuals.MiRNA- 155 expression level showed significant positive correlation with 24 h Daytime SBP（r =0.681,P 〈 0.001）,24 h Daytime DBP（r = 0.473,P 〈 0.001）,24 h Daytime PP（r = 0.565,P 〈 0.001） and dipping（r = 0.257,P = 0.018）,respectively.Conclusions Our study showed that mi RNA-155 expression level was associated positively with daytime blood pressure monitoring parameters,as well as blood pressure variability,indicating a possible implication of miRNA- 155 in the pathogenesis of hypertension.
基金supported by the grants from Guangdong Natural Science Foundation(No.S2013010016575/No.2015A030313660)the Technology Project Foundation of Guangzhou(No.2014y2-00140/No.1563000381)the Technology Project Foundation of Guangdong Province(No.2014B020212008)
文摘Background Micro RNAs have recently been considered as biomarkers in several different cardiovascular diseases,however,so far there are no circulating miRNAs data about hypertension.Therefore,the aim of the present pilot study was to identify circulating miRNAs for hypertension biomarkers.Methods Using an Agilent microarray,plasma miRNAs were profiled from plasma samples of 10 patients with untreated essential hypertension and 10 healthy controls.Candidate biomarkers identified in the profiles were subjected to validation by using quantitative PCR in an independent sample set of 20 patients with untreated essential hypertension and 20 healthy controls.Then,we assessed the selected miRNAs for the detection and diagnosis of hypertension from plasma samples of 70 patients with untreated essential hypertension and 20 healthy controls.The Spearman correlation coefficient was used to assessed the selected miRNAs correlations with blood pressure.The area under the receiver operating characteristic curve（AUC） was used to evaluate diagnostic accuracy.Results The expressions of selected 8 miRNAs were investigated independently in plasma samples from 10 hypertension patients and 10 healthy subjects.The levels of circulating miR-30c-5p,miR-133 b,miR-29b-3p,miR-29a-3p,miR-29c-3p,miR-30a-3p,miR-let7b-3p expression were significantly down regulated in hypertension group compared with healthy group and the level of hsa-miR-92b-3p was significantly unregulated between the groups.We used q RT-PCR assay to confirm the expression of 8 candidate miRNAs,miR-30c-5p（P 〈 0.001）,miR-29b-3p（P 〈 0.001）,miR-29a-3p（P = 0.027）,miR-29c-3p（P 〈 0.001）,miR-92b-3p（P = 0.003）,miR-30a-3p（P = 0.704）,miR-133b（P =0.346）,andmiR-let7b（P = 0.161）.The diagnostic accuracy of miR-30c-5p,miR-29b-3p,miR-29a-3p,miR-29c-3p and miR-92b-3p,as measured by AUC,were 0.897,0.90,0.829,0.825 and 0.832,respectively,with all P 〈0.001.Conclusions The plasma levels of miR-30c-5p,miR-29b-3p,miR-29a-3p,miR-29c-3p and miR-92b-3p associated with hypertension which provide an impetus for future evaluations of hypertension development,progression,therapeutic efficacy and prognosis.
